Twin trawling for shrimp with dummy doors

Twin trawling; a new concept in shrimp trawling is described in this paper. It essentially involves towing of two trawls of equal size connected in sequence at the inner head and foot rope legs and using a pair of outer otter boards and central neutral door or dummy door with triple bridles and single tow warp. A comparative study with conventional stern trawling using a standard 13.7 m four seam shrimp trawl and two 6 8 m standard four seam trawls as twin-rig has shown considerable reduction in the utilization of power (20 %) with better efficiency in shrimp fishing.

On the performance of otter trawls operated from a 15.4 m trawler off Veraval

Results obtained with three trawl nets namely, 13.7m four seam, 15.8m six seam and 18.3m four seam operated from a 15.4m wooden trawler, are presented. Among the three nets operated, 15.8m six seam trawl and 18.3 four seam trawl were found to be equally effective for the capture of shrimp and during the lean season 15.8m six seam trawl can be successfully operated to exploit demersal as well as semi-pelagic fish resources. The catch data were analysed using analysis of variance and Gulland's method.

A demersal trawling gear for inshore waters along the west coast

Results of comprehensive efficiency tests of three tested designs of 15m bulged belly, 15.8m six seam and 29.26m longwing type trawls in combination with 114x57cm both rectangular flat and horizontal curved wooden otter boards are reported. Of the possible six combinations, the bulged-belly trawl with flat rectangular otter boards has performed better in landing prawns. Further this study has indicated the selective action of the different designs.

Tin coating requirements in fish cans

Prawn, crab and clam meat were processed in experimental ca s having reduced internal tin coating of 5.6 GSM. Conventional cans having 11.2 GSM tin coating were used as control. Results showed that experimental cans behaved normally when used for canning prawns, provided the lacquer film was perfect with no exposure of metal. When there was a discontinuity in lacquer film exposing the metal blackening took place in such areas. Areas subjected to severe strains like the lock seam side and expansion rings on can ends were found to be more prone to blackening. Experimental cans were found unsuitable for canning crab meat or clam meat because in both cases the can wall as well as the contents underwent discoloration, in all cases.

Viability and performance of demountable composite connectors

Material production, and associated carbon emissions, could be reduced by reusing products instead of landfilling or recycling them. Steel beams are well suited to reuse, but are difficult to reuse when connected compositely to concrete slabs using welded studs. A demountable connection would allow composite performance but also permit reuse of both components at end-of-life. Three composite beams, of 2 m, 10 m and 5 m length, are constructed using M20 bolts as demountable shear connectors. The beams are tested in three-, six- and four-point bending, respectively. The former two are loaded to service, unloaded, demounted and reassembled; all three are tested to failure. The results show that all three have higher strengths than predicted using Eurocode 4. The longer specimens have performance similar to previously published comparable welded-connector composite beam results. This suggests that demountable composite beams can be safely used and practically reused, thus reducing carbon emissions. © 2013 Elsevier B.V. All rights reserved.

SEA的基因克隆及高效表达工程菌的构建

Staphylococcal enterotoxin A(SEA）是由金黄色葡萄球菌分泌至胞外的一种单亚基蛋白质。它能与抗原递呈细胞上的MHC II受体结合，激发大量T细胞活化，释放多种细胞因子。正是由于SEA是一种强的T细胞激活剂，近年来，它在肿瘤的免疫治疗中显示出巨大的应用潜力。本文总结了国内外近十几年来在SEA治疗肿瘤方面的研究成果，指出，SEA靶向药物及低毒性SEA分子改造是SEA类药物研究的方向。利用PCR方法从金葡菌基因组DNA中扩增出seap基因（即SEA前体基因，含信号肤编码区），采用重叠PCR技术突变了694佩714碱基之间的所有稀有密码子，得到seap，。基因表达结果证实，改造seap基因中稀有密码子对提高基因表达水平非常有效。对seap基因中关键稀有密码子的突变在国内外尚属首次。以seapm为模板采用PCR方法扩增出seam (SEA成熟蛋白基因）。自行设计，自行构建成功一个新型原核表达载体7ZTS。它具有克隆，测序和表达在同一载体上进行，并可依据蓝白反应挑选重组子的特征。7ZTS表达载体将多种功能集于一体的独特优点是目前已知表达载体所不具备的，其大大简化了分子生物学操作步骤，提高了工作效率。利用7ZTS表达载体，在大肠杆菌JM109（DE3)中表达了mseap和seam基因。前者以包含体形式表达出SEA前体蛋白，后者以包含体和可溶性两种形式存在。通过均匀设计方法，摸索出'0sea基因表达的最佳条件为：诱导前生物量ODEo。为0. 8，装液量为28%，诱导温度为37'C，诱导时间为8小时。在此条件下，SEA表达量占细胞总蛋白的37%，可溶性SEA约占70%o SEA如此高效的表达为国内一外首次报道。质粒稳定性实验表明，7ZTS一msea重组质粒具有极好的稳定性，连续培养72小时或IPTG诱导48小时后，质粒基本不丢失。以上实验结果为SEA的大规模制备和SEA导向药物的研究开发奠定了基础。

IL-2基因的突变及其与肠毒素融合基因的克隆和表达

由金黄色葡萄球菌分泌到胞外的单亚基蛋白中有肠毒素A和B。近年来两种毒素在肿瘤治疗研究方面取得了很大进展。白介素-2作为靶向分子，在抗肿瘤的药物中很有应用前景。本文分别对肠毒素A、肠毒素B和白介素-2进行了克隆和表达，并将白介素-2（125Ala）分别与肠毒素A227AI。肠毒素B进行了融合表达。从筛选到的天然金黄色葡萄球菌STSw的基因组中，通过PCR方法扩增出seb基因，同时突变了该基因两端几个稀有密码子。并将其克隆到7ZTS载体上进行表达，表达量占细胞总蛋白的33.5％。以seam基因为模板，通过重叠PCR将其227位天冬氨酸突变为丙氨酸，以降低其毒性。该突变基因重组到7ZTS载体中，并在JM109（DE3）中表达，表达量占细胞总蛋白的51.5％。通过重叠PCR法，对人的IL-2基因进行定点突变。共突变60个碱基，涉及51个氨基酸，其中第125位的半肤氨酸被突变为丙氨酸。该基因在大肠杆菌中表达量占总蛋白的30％。分别对上述三个工程菌的表达条件进行了探索。先制备出融合基因，再对融合基因进行表达，得到两种融合蛋白。它们是以6个甘氨酸和1个苏氨酸为链，将IL一2（125Ala）与肠毒素A227（Ala）、B分别连接起来，即IL-2（125Ala）-SEA227（Ala）和IL-2（125Ala）-SEB二者在大肠杆菌中表达量分别占总蛋白的10％和12％。以上实验结果为将几种蛋白开发成抗肿瘤靶向药物奠定了坚实基础。

不锈钢激光搭接焊接头温度场数值模拟及分析

温度场是影响激光焊接焊缝成形质量的关键因素. 针对非熔透型激光搭接焊接头焊缝成"钉头"状的特点,通过分析焊接时材料吸收激光能量的分布情况,提出了高斯面热源加线性递增式柱热源的复合体热源模型. 模型考虑板间接触热阻的影响,并将计算结果和试验结果进行了对比,发现模拟出的焊缝形状和试验吻合较好;此外基于本模型对焊缝各处的热循环与焊缝组织形貌及显微硬度的关系进行了分析. 结果表明,焊缝组织形貌及显微硬度除与加热和冷却速率有关外,峰值温度对其也有重要影响;在热循环基本一致的情况下焊缝的性能相似. 该模型较准确地模拟了薄板激光深熔焊接熔池温度场,对研究激光深熔焊接温度场问题和激光工艺参数的优化选择具有参考价值

Magnets for HIRFL-CSR rings

The magnet design, fabrication, and measurement of HIRFL-CSR (Heavy Ion Research Facility in Lanzhou Cooling Storage Ring) are presented. All magnets will be laminated And welded with an armor-coated surface between two big endplates made of sticking glue 0.5 mm-thick sheets. The dipole of CSRm was chosen an H type with an air circle on the pole to improve the field uniformity. The dipole of CSRe was chosen the C type with an air circle and two air slots on the pole to improve the field homogeneity. Its reproducibility of magnet to magnet was adjusted with inserting small laminating pieces before demountable pole ends to reach less than +/- 2 x 10(-4) at optimized field level. CSRm quadrupoles diameter is 170 mm and has two different lengths, and its endplates were made with punching pieces after coating with epoxy glue, there is chamfered directly on the pole ends to reduce 12th-order contribution of field and without the demountable pole ends. CSRe main quadrupoles diameter is 240 mm and has two different lengths, and its endplates were also made with punching pieces coated with epoxy glue, there is also chamfered directly on the pole ends to reduce 12th-order contribution of field like CSRm.

改造稀有密码子提高SEA蛋白表达量

利用重叠PCR技术突变了sea基因上一个稀有密码子簇 ,将此段中稀有密码子全部更换成E .coli最常用密码子 ,得到seam 。将sea和seam 分别克隆于 7ZTS表达载体上 ,并转化JM10 9(DE3)菌株。结果表明 ,sea基因的表达十分微弱 ,而seam 基因的表达量十分高 ,约占菌体总蛋白的 15 %。表达产物在体内具有一定的抗肿瘤活性。

塑料压力管道热板焊接接头瞬时应力有限元分析

基于热粘弹性积分型本构关系,考虑材料性能依赖于温度变化及相变潜热的影响,利用有限元软件ANSYS热-力耦合及载荷步功能模拟结晶型高密度聚乙烯塑料压力管道热板焊接过程。并对焊接接头的应力分布进行有限元分析,得到了环向、轴向及径向瞬态应力分布规律。采用盲孔法和锯切法测量焊后残余应力,实测结果与数值分析基本吻合。

高密度聚乙烯塑料压力管道热板焊接头应力分布有限元分析

基于热粘弹性积分型本构关系,考虑材料性能依赖于温度变化及相变潜热的影响,利用AN SYS热-力耦合及载荷步功能模拟结晶型高密度聚乙烯(HDPE)塑料压力管道热板焊接过程,并对焊接接头的应力分布进行有限元分析,得到了环向、轴向以及径向瞬态应力分布的基本规律。采用盲孔法和锯切法测量焊后残余应力,实测结果与数值分析基本符合

大型激光叠焊系统研究与开发

介绍一种新型激光叠焊系统,利用该系统可将尺寸范围为12.0m×1.5m厚度不等的两块不锈钢板重叠焊接在一起。针对大尺寸不锈钢板尺寸大、易变形等特点提出了主—辅式压紧机构,有效地控制了焊接过程中大尺寸板材的变形并消除了板材间隙;设计了ufxyz型四轴联动方案:u轴与x轴同向,u轴驱动钢板进行大范围运动,x轴驱动激光头小范围运动。u轴与x轴的复合运动提高系统精度的同时提高了系统焊接动态响应速度。对实际焊接板材进行试验验证,结果表明使用该系统进行焊接的钢板可承受压强最大能达到6.1MPa。

激光拼焊错边产生与预测建模方法

错边是评定激光拼焊质量的一个非常重要的指标,薄板构件错边的控制是激光拼焊中一个难题。针对国内首条全自动激光拼焊设备,对错边的产生以及控制方法进行了深入研究,经过大量试验确定了影响错边大小的几个主要因素,板材自身物理变形、压紧力大小与均匀性、压紧横梁变形、支撑底板平面度误差以及焊接变形的影响。通过分析以上因素对错边的影响以及各个因素之间相互关系,建立了错边预测的数学模型。试验验证了模型的正确性,从而为确定错边产生原因,提高焊接质量提供了一个有效的理论指导。

基于Zernike矩边缘检测的焊缝信息获取

针对目前焊缝坐标提取方法存在精度较低,难于实现视觉引导的机器人激光焊接高速度、高精度的要求,提出一种基于Zernike正交矩的曲线焊缝位置坐标信息获取算法,该算法首先采用Zernike边缘检测算法识别焊缝边缘,然后提取出焊缝的中心线,最后计算出该中心线的亚像素坐标。通过试验验证了该算法的可行性。