As relações no processo de montagem cinematográfica entre os filmes Um homem com uma câmera e Koyaanisqatsi

Pós-graduação em Comunicação - FAAC

A produção independente de webséries pela perspectiva multiplataforma da televisão digital e internet

Pós-graduação em Televisão Digital: Informação e Conhecimento - FAAC

Possibilidades e limites de experiência: imagem e formação de professoras de infância da rede pública de Rio Claro

Pós-graduação em Educação - IBRC

Comparação entre achados clínicos e videofluoroscópicos na identificação de penetração laríngea e aspiração laringotraqueal no acidente vascular encefálico

Pós-graduação em Bases Gerais da Cirurgia - FMB

Análise comparativa do uso da tabela fonética do português brasileiro cantado por cantores argentinos com e sem o uso de um recurso áudio visual

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Luzes e sombras: Paulo Emilio Salles Gomes e a cultura cinematográfica (1954-59)

Pós-graduação em História - FCLAS

Três trajetórias e uma mesma estrada

The process of migration of the rural northeast workers toward the cities of the south of Brazil in search of work and better living conditions reached millions of individuals and constituted the basis of our industrialization and modernization, especially since the 1930s. The Cinema has not ignored this vast phenomenon and, as much as the literature put the question in debate, particularly since 1960, when our seventh art was assumed as part of the national intelligence. In this article, we observed three narratives that thematize the escape from rural to urban from the northeastern hinterland: Vidas Secas (1963), by Nelson Pereira dos Santos, Trópicos (BR / ITA, 1967), by Gianni Amico, and O Caminho das Nuvens (2003), by Vicente Amorim. Participants of the politicized cinema, Vidas Secas and Trópicos draw the political impasses of the period in the form of movies and in the aesthetic choices, trying to understand the role of these migrants in Brazilian life. Already in O Caminho das Núvens, performed four decades later, the narrative is assumed as part of the cinema of entertainment, thinking the process of migration as a choice, as stated the form of this artwork of the called cinema of the resumption.

Nós falamos da vida

It is said that a film producer to receive a prestigious award in the United States thanked saying We Speak of Life. Maybe this isone of the reasons for the fascination produced by this art. Life is a movie. The film celebrates life. The book We Speak of Lifeshows who are professionals of the cinema exhibition, those who are in the small microcosm that is the projection room and usuallyare not remembered by the general public

O biodireito na interface entre ciência e ficção: um estudo do imaginário social a partir da análise do filme Mar adentro

Pós-graduação em Educação - IBRC

Pérola gera arte: (re)ações entre gêneros

This paper is part of a larger project. The purpose of this paper is to discuss the movie Girl With a Pearl Earring (2003) by Peter Webber using the theoretical perspective of dialogue from the Bakhtin Circle, Medved, Volochinov. The multidisciplinary and interartistic potential of cinema is considered in this paper when the linguistic organization and the translinguistic elements in the corpus are focused on and understood as an example of cinema genre as discussed in the Russian Circle. The study of the ways of incorporation of different genericities by cinema, illustrated by the dialogues between the pictorial genre, the novel and the movies contribute to the recognition of the cinema as a genre that is composed by the incorporation of other genres. The construction of the cinematographic work in analysis will be regarded in its architectonic (form, content and style) and in the activity field in which it circulates.

Anti-dementia medications: current prescriptions in clinical practice and new agents in progress

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Todos os filmes estrangeiros

O artigo toma as figuras da legenda e da dublagem como pontos de partida para a exploração das várias formas de estrangeirice no audiovisual. Tais figuras são apenas as marcas mais visíveis e impositivas da maneira como os filmes envolvem questões relacionadas com a diferença, a alteridade e a tradução. Em seguida, o artigo também discute o que pode ser um filme estrangeiro no contexto da globalização e da internacionalização da produção cinematográfica. Enfim, ele invoca o conceito de «cinema com sotaque», ou seja, o cinema criado ao mesmo tempo nos interstícios das formações sociais e das práticas cinematográficas, especialmente nas comunidades exílicas e em situações de diáspora.

Impatto di rifaximina sul microbiota intestinale: selezione di bifidobatteri antibiotico resistenti

The ideal approach for the long term treatment of intestinal disorders, such as inflammatory bowel disease (IBD), is represented by a safe and well tolerated therapy able to reduce mucosal inflammation and maintain homeostasis of the intestinal microbiota. A combined therapy with antimicrobial agents, to reduce antigenic load, and immunomodulators, to ameliorate the dysregulated responses, followed by probiotic supplementation has been proposed. Because of the complementary mechanisms of action of antibiotics and probiotics, a combined therapeutic approach would give advantages in terms of enlargement of the antimicrobial spectrum, due to the barrier effect of probiotic bacteria, and limitation of some side effects of traditional chemiotherapy (i.e. indiscriminate decrease of aggressive and protective intestinal bacteria, altered absorption of nutrient elements, allergic and inflammatory reactions). Rifaximin (4-deoxy-4’-methylpyrido[1’,2’-1,2]imidazo[5,4-c]rifamycin SV) is a product of synthesis experiments designed to modify the parent compound, rifamycin, in order to achieve low gastrointestinal absorption while retaining good antibacterial activity. Both experimental and clinical pharmacology clearly show that this compound is a non systemic antibiotic with a broad spectrum of antibacterial action, covering Gram-positive and Gram-negative organisms, both aerobes and anaerobes. Being virtually non absorbed, its bioavailability within the gastrointestinal tract is rather high with intraluminal and faecal drug concentrations that largely exceed the MIC values observed in vitro against a wide range of pathogenic microorganisms. The gastrointestinal tract represents therefore the primary therapeutic target and gastrointestinal infections the main indication. The little value of rifaximin outside the enteric area minimizes both antimicrobial resistance and systemic adverse events. Fermented dairy products enriched with probiotic bacteria have developed into one of the most successful categories of functional foods. Probiotics are defined as “live microorganisms which, when administered in adequate amounts, confer a health benefit on the host” (FAO/WHO, 2002), and mainly include Lactobacillus and Bifidobacterium species. Probiotic bacteria exert a direct effect on the intestinal microbiota of the host and contribute to organoleptic, rheological and nutritional properties of food. Administration of pharmaceutical probiotic formula has been associated with therapeutic effects in treatment of diarrhoea, constipation, flatulence, enteropathogens colonization, gastroenteritis, hypercholesterolemia, IBD, such as ulcerative colitis (UC), Crohn’s disease, pouchitis and irritable bowel syndrome. Prerequisites for probiotics are to be effective and safe. The characteristics of an effective probiotic for gastrointestinal tract disorders are tolerance to upper gastrointestinal environment (resistance to digestion by enteric or pancreatic enzymes, gastric acid and bile), adhesion on intestinal surface to lengthen the retention time, ability to prevent the adherence, establishment and/or replication of pathogens, production of antimicrobial substances, degradation of toxic catabolites by bacterial detoxifying enzymatic activities, and modulation of the host immune responses. This study was carried out using a validated three-stage fermentative continuous system and it is aimed to investigate the effect of rifaximin on the colonic microbial flora of a healthy individual, in terms of bacterial composition and production of fermentative metabolic end products. Moreover, this is the first study that investigates in vitro the impact of the simultaneous administration of the antibiotic rifaximin and the probiotic B. lactis BI07 on the intestinal microbiota. Bacterial groups of interest were evaluated using culture-based methods and molecular culture-independent techniques (FISH, PCR-DGGE). Metabolic outputs in terms of SCFA profiles were determined by HPLC analysis. Collected data demonstrated that rifaximin as well as antibiotic and probiotic treatment did not change drastically the intestinal microflora, whereas bacteria belonging to Bifidobacterium and Lactobacillus significantly increase over the course of the treatment, suggesting a spontaneous upsurge of rifaximin resistance. These results are in agreement with a previous study, in which it has been demonstrated that rifaximin administration in patients with UC, affects the host with minor variations of the intestinal microflora, and that the microbiota is restored over a wash-out period. In particular, several Bifidobacterium rifaximin resistant mutants could be isolated during the antibiotic treatment, but they disappeared after the antibiotic suspension. Furthermore, bacteria belonging to Atopobium spp. and E. rectale/Clostridium cluster XIVa increased significantly after rifaximin and probiotic treatment. Atopobium genus and E. rectale/Clostridium cluster XIVa are saccharolytic, butyrate-producing bacteria, and for these characteristics they are widely considered health-promoting microorganisms. The absence of major variations in the intestinal microflora of a healthy individual and the significant increase in probiotic and health-promoting bacteria concentrations support the rationale of the administration of rifaximin as efficacious and non-dysbiosis promoting therapy and suggest the efficacy of an antibiotic/probiotic combined treatment in several gut pathologies, such as IBD. To assess the use of an antibiotic/probiotic combination for clinical management of intestinal disorders, genetic, proteomic and physiologic approaches were employed to elucidate molecular mechanisms determining rifaximin resistance in Bifidobacterium, and the expected interactions occurring in the gut between these bacteria and the drug. The ability of an antimicrobial agent to select resistance is a relevant factor that affects its usefulness and may diminish its useful life. Rifaximin resistance phenotype was easily acquired by all bifidobacteria analyzed [type strains of the most representative intestinal bifidobacterial species (B. infantis, B. breve, B. longum, B. adolescentis and B. bifidum) and three bifidobacteria included in a pharmaceutical probiotic preparation (B. lactis BI07, B. breve BBSF and B. longum BL04)] and persisted for more than 400 bacterial generations in the absence of selective pressure. Exclusion of any reversion phenomenon suggested two hypotheses: (i) stable and immobile genetic elements encode resistance; (ii) the drug moiety does not act as an inducer of the resistance phenotype, but enables selection of resistant mutants. Since point mutations in rpoB have been indicated as representing the principal factor determining rifampicin resistance in E. coli and M. tuberculosis, whether a similar mechanism also occurs in Bifidobacterium was verified. The analysis of a 129 bp rpoB core region of several wild-type and resistant bifidobacteria revealed five different types of miss-sense mutations in codons 513, 516, 522 and 529. Position 529 was a novel mutation site, not previously described, and position 522 appeared interesting for both the double point substitutions and the heterogeneous profile of nucleotide changes. The sequence heterogeneity of codon 522 in Bifidobacterium leads to hypothesize an indirect role of its encoded amino acid in the binding with the rifaximin moiety. These results demonstrated the chromosomal nature of rifaximin resistance in Bifidobacterium, minimizing risk factors for horizontal transmission of resistance elements between intestinal microbial species. Further proteomic and physiologic investigations were carried out using B. lactis BI07, component of a pharmaceutical probiotic preparation, as a model strain. The choice of this strain was determined based on the following elements: (i) B. lactis BI07 is able to survive and persist in the gut; (ii) a proteomic overview of this strain has been recently reported. The involvement of metabolic changes associated with rifaximin resistance was investigated by proteomic analysis performed with two-dimensional electrophoresis and mass spectrometry. Comparative proteomic mapping of BI07-wt and BI07-res revealed that most differences in protein expression patterns were genetically encoded rather than induced by antibiotic exposure. In particular, rifaximin resistance phenotype was characterized by increased expression levels of stress proteins. Overexpression of stress proteins was expected, as they represent a common non specific response by bacteria when stimulated by different shock conditions, including exposure to toxic agents like heavy metals, oxidants, acids, bile salts and antibiotics. Also, positive transcription regulators were found to be overexpressed in BI07-res, suggesting that bacteria could activate compensatory mechanisms to assist the transcription process in the presence of RNA polymerase inhibitors. Other differences in expression profiles were related to proteins involved in central metabolism; these modifications suggest metabolic disadvantages of resistant mutants in comparison with sensitive bifidobacteria in the gut environment, without selective pressure, explaining their disappearance from faeces of patients with UC after interruption of antibiotic treatment. The differences observed between BI07-wt e BI07-res proteomic patterns, as well as the high frequency of silent mutations reported for resistant mutants of Bifidobacterium could be the consequences of an increased mutation rate, mechanism which may lead to persistence of resistant bacteria in the population. However, the in vivo disappearance of resistant mutants in absence of selective pressure, allows excluding the upsurge of compensatory mutations without loss of resistance. Furthermore, the proteomic characterization of the resistant phenotype suggests that rifaximin resistance is associated with a reduced bacterial fitness in B. lactis BI07-res, supporting the hypothesis of a biological cost of antibiotic resistance in Bifidobacterium. The hypothesis of rifaximin inactivation by bacterial enzymatic activities was verified by using liquid chromatography coupled with tandem mass spectrometry. Neither chemical modifications nor degradation derivatives of the rifaximin moiety were detected. The exclusion of a biodegradation pattern for the drug was further supported by the quantitative recovery in BI07-res culture fractions of the total rifaximin amount (100 μg/ml) added to the culture medium. To confirm the main role of the mutation on the β chain of RNA polymerase in rifaximin resistance acquisition, transcription activity of crude enzymatic extracts of BI07-res cells was evaluated. Although the inhibition effects of rifaximin on in vitro transcription were definitely higher for BI07-wt than for BI07-res, a partial resistance of the mutated RNA polymerase at rifaximin concentrations > 10 μg/ml was supposed, on the basis of the calculated differences in inhibition percentages between BI07-wt and BI07-res. By considering the resistance of entire BI07-res cells to rifaximin concentrations > 100 μg/ml, supplementary resistance mechanisms may take place in vivo. A barrier for the rifaximin uptake in BI07-res cells was suggested in this study, on the basis of the major portion of the antibiotic found to be bound to the cellular pellet respect to the portion recovered in the cellular lysate. Related to this finding, a resistance mechanism involving changes of membrane permeability was supposed. A previous study supports this hypothesis, demonstrating the involvement of surface properties and permeability in natural resistance to rifampicin in mycobacteria, isolated from cases of human infection, which possessed a rifampicin-susceptible RNA polymerase. To understand the mechanism of membrane barrier, variations in percentage of saturated and unsaturated FAs and their methylation products in BI07-wt and BI07-res membranes were investigated. While saturated FAs confer rigidity to membrane and resistance to stress agents, such as antibiotics, a high level of lipid unsaturation is associated with high fluidity and susceptibility to stresses. Thus, the higher percentage of saturated FAs during the stationary phase of BI07-res could represent a defence mechanism of mutant cells to prevent the antibiotic uptake. Furthermore, the increase of CFAs such as dihydrosterculic acid during the stationary phase of BI07-res suggests that this CFA could be more suitable than its isomer lactobacillic acid to interact with and prevent the penetration of exogenous molecules including rifaximin. Finally, the impact of rifaximin on immune regulatory functions of the gut was evaluated. It has been suggested a potential anti-inflammatory effect of rifaximin, with reduced secretion of IFN-γ in a rodent model of colitis. Analogously, it has been reported a significant decrease in IL-8, MCP-1, MCP-3 e IL-10 levels in patients affected by pouchitis, treated with a combined therapy of rifaximin and ciprofloxacin. Since rifaximin enables in vivo and in vitro selection of Bifidobacterium resistant mutants with high frequency, the immunomodulation activities of rifaximin associated with a B. lactis resistant mutant were also taken into account. Data obtained from PBMC stimulation experiments suggest the following conclusions: (i) rifaximin does not exert any effect on production of IL-1β, IL-6 and IL-10, whereas it weakly stimulates production of TNF-α; (ii) B. lactis appears as a good inducer of IL-1β, IL-6 and TNF-α; (iii) combination of BI07-res and rifaximin exhibits a lower stimulation effect than BI07-res alone, especially for IL-6. These results confirm the potential anti-inflammatory effect of rifaximin, and are in agreement with several studies that report a transient pro-inflammatory response associated with probiotic administration. The understanding of the molecular factors determining rifaximin resistance in the genus Bifidobacterium assumes an applicative significance at pharmaceutical and medical level, as it represents the scientific basis to justify the simultaneous use of the antibiotic rifaximin and probiotic bifidobacteria in the clinical treatment of intestinal disorders.

Nuovi suoni di balenottera comune (Balaenoptera physalus) nel Mediterraneo occidentale

Fin whale (Balaenoptera physalus) is the only misticeto commonly observed in the Mediterranean. The males emit sounds, classic pulse products in sequences called songs, at 20Hz for sexual purposes: sounds are produced during the spring for migration to the Tirreno-Ligurian-Provençal basin, the summer feeding area, and during the autumn, when there is a migration to the south to meet the winter breeding season. This area in the Mediterranean sea is unknown. The east coast of the Iberian Peninsula is a migration area. The study was conducted by analyzing through Adobe Audition 3.0 and XBAT softwares files audio of 30 minutes recorded in 2006, in 2011 and 2012 at the level of the Columbretes Islands, in the western Mediterranean sea, using two hearing aids: the MARU, used in 2006 and the EAR, used in 2011 and 2012. From the analysis have emerged that, in addition to songs with pulses of 20 Hz, there are new sounds of fin whale never previously recognized: the VFPs (Variable Frequency Pulses), higher-frequency pulses emitted, between 50 and 120Hz and the ramps, a set of 7-8 pulses, pertaining to a particular song, of increasing frequency. Further studies are needed to understand the importance of these new sounds.

Efectos sonoros en el canto IX de Ilíada

Al considerar los procedimientos poéticos que aparecen en los textos homéricos, es fundamental no perder de vista las posibilidades significativas que pueden presentar para la recepción auditiva propia de la performance épica. En la recreación de los acontecimientos míticos, los elementos del lenguaje específicamente poético cumplen una función instauradora esencial, pues al transformar los procedimientos poéticos en creencias perceptivas, realizan la presencia de los hechos narrados. Así es que procedimientos tales como la aliteración conllevan una carga temática que los convierte en un rasgo clave de la poética homérica. Los efectos sonoros en los textos homéricos fueron comentados desde la antigüedad, especialmente la capacidad de reproducir verbalmente sonidos de varios tipos de fenómenos naturales, seres y objetos. En los casos del canto IX de Ilíada, tales elementos cumplen una función relevante para la trama a través del leitmotiv tradicional que contribuyen a instaurar. Este tipo de análisis basado en las recurrencias de efectos sonoros permite recuperar para la lectura actual un rasgo importante de la poesía homérica: la 'creación verbal con motivación onomatopéyica'.