Feasibility of in vivo 15N MRS detection of hyperpolarized 15N labeled choline in rats.

The increase of total choline in tumors has become an important biomarker in cancer diagnosis. Choline and choline metabolites can be measured in vivo and in vitro using multinuclear MRS. Recent in vivo(13)C MRS studies using labeled substrates enhanced via dynamic nuclear polarization demonstrated the tremendous potential of hyperpolarization for real-time metabolic studies. The present study demonstrates the feasibility of detecting hyperpolarized (15)N labeled choline in vivo in a rat head at 9.4 T. We furthermore report the in vitro (172 +/- 16 s) and in vivo (126 +/- 15 s) longitudinal relaxation times. We conclude that with appropriate infusion protocols it is feasible to detect hyperpolarized (15)N labeled choline in live animals.

Acid-sensing channels in human bladder: expression, function and alterations during bladder pain syndrome.

Purpose: To examine the possible role of H+-activated acid-sensing ion channels (ASICs) in pain perception we characterized their expression in bladder dome biopsies of Bladder Pain Syndrome (BPS) patients and controls, in cultured human urothelium and in urothelial TEU-2 cells.Materials and Methods: Cold cut biopsies from the bladder dome were obtained in 8 asymptomatic controls and 28 patients with symptoms of BPS. ASIC expression was analyzed by QPCR and immunofluorescence. The channel function was measured by electrophysiology.Results: ASIC1a, ASIC2a and ASIC3 mRNAs were detected in human bladder. Similar amounts of ASIC1a and -3 were detected in detrusor smooth muscle, whereas in urothelium ASIC3 levels were higher than -1a. ASIC2a mRNA levels were lower than either -1a or -3 in both layers. ASIC currents were measured in TEU-2 cells and in primary cultures of human urothelium, and ASIC expression was confirmed by QPCR. Differentiation of TEU-2 cells caused an up-regulation of ASIC2a and ASIC3, and a down-regulation of ASIC1a mRNAs. BPS patients showed an up-regulation of ASIC2a and -3 mRNA, whereas ASIC1a remained unchanged. In contrast, the mRNA levels of TRPV1 were down-regulated during BPS. All differences were statistically significant (p<0.05)Conclusions: Several different ASIC subunits are expressed in human bladder and TEU-2 cells, where their levels are regulated during urothelial differentiation. An up-regulation of ASIC2a and -3 in BPS suggests their involvement in increased pain and hyperalgesia. A down-regulation of TRPV1 mRNA levels might indicate a different regulatory mechanism, controlling its expression in human bladder.

Apocalypsis

[Bible. N.T.. Apocalypse (latin). circa 1462-1463. figures]

Joannis Joviani Pontani Opera omnia, soluta oratione composita Nomina librorum qui in hisse tribus partibus continentur, à tergo hujus paginae notatae reperiuntur

Comprend : Centum Ptolemaei Sententiae ad Syrum fratrem a Pontano è graeco in latinum tralatae, atque expositae

Joannis Joviani Pontani Opera omnia, soluta oratione composita Nomina librorum qui in hisse tribus partibus continentur, à tergo hujus paginae notatae reperiuntur

Comprend : Centum Ptolemaei Sententiae ad Syrum fratrem a Pontano è graeco in latinum tralatae, atque expositae

No t'emocionis...Escolta! L'ús de la música en l'educació emocional

Usos i propostes de la música en l'educació emocional

Educació en valors i educació musical

L'educació musical com a eina eficaç en l'educació en valors.

Reliure de : Les oeuvres de François Villon

Numérisation partielle de reliure

Neural regulation of pancreatic islet cell mass and function.

Intracellular glucose signalling pathways control the secretion of glucagon and insulin by pancreatic islet α- and β-cells, respectively. However, glucose also indirectly controls the secretion of these hormones through regulation of the autonomic nervous system that richly innervates this endocrine organ. Both parasympathetic and sympathetic nervous systems also impact endocrine pancreas postnatal development and plasticity in adult animals. Defects in these autonomic regulations impair β-cell mass expansion during the weaning period and β-cell mass adaptation in adult life. Both branches of the autonomic nervous system also regulate glucagon secretion. In type 2 diabetes, impaired glucose-dependent autonomic activity causes the loss of cephalic and first phases of insulin secretion, and impaired suppression of glucagon secretion in the postabsorptive phase; in diabetic patients treated with insulin, it causes a progressive failure of hypoglycaemia to trigger the secretion of glucagon and other counterregulatory hormones. Therefore, identification of the glucose-sensing cells that control the autonomic innervation of the endocrine pancreatic and insulin and glucagon secretion is an important goal of research. This is required for a better understanding of the physiological control of glucose homeostasis and its deregulation in diabetes. This review will discuss recent advances in this field of investigation.

[Illustrations du Grant testament.] / François Villon, aut. du texte

Comprend : [Fig. en reg. titre : l'évêque Thibault d'Aussigny]. [Cote : microfilm R 9751] ; [2ème fig. en reg. p. VIIII [IX] : François Villon]. [Cote : microfilm R 9751] ; [3ème fig. p. VIIII [IX] :] La vieille en regrettant le temps de sa jeunesse. [Cote : microfilm R 9751] ; [4ème fig. :] Villon. [identique à la 2ème fig. mais avec ornements différents]. [Cote : microfilm R 9751] ; [5ème fig. :] Beaulte damours. [Cote : microfilm R 9751] ; [6ème fig. :] Villon. [Cote : microfilm R 9751] ; [7ème fig. :] La grosse Margot. [identique à la 3ème fig. mais avec ornements différents]. [Cote : microfilm R 9751]

[Illustrations de Le Grant testament Villon et le petit] / [Non identifié] ; François Villon, aut. du texte

Comprend : [Fig. au titre : monogramme.] [Cote : Res Ye 245/Microfilm R 727] ; [Fig. en reg. folio a ii : François Villon.] [Cote : Res Ye 245/Microfilm R 727] ; [Fig. folio a ii : un évêque.] Leuesque. [Cote : Res Ye 245/Microfilm R 727] ; [Fig. en reg. folio b iiii : François Villon.] Côment Villon voit a son aduis la belle heaulmier soy côplaigant. [Cote : Res Ye 245/Microfilm R 727] ; [Fig. folio b iiii : une vieille femme.] La vieille en regrettant le temps de sa ieunesse. [Cote : Res Ye 245/Microfilm R 727] ; [Fig. : François Villon.] [Cote : Res Ye 245/Microfilm R 727] ; [Fig. : une jeune femme, Beauté d'Amour.] Beaulte damours. [Cote : Res Ye 245/Microfilm R 727] ; [Fig. : François Villon.] [Cote : Res Ye 245/Microfilm R 727] ; [Fig. : une femme, la grosse Margot.] La grosse margot. [Cote : Res Ye 245/Microfilm R 727] ; [Fig.en reg. folio g iii : François Villon.] Le rondeau que feist ledit Villon quant il fut iugie. [Cote : Res Ye 245/Microfilm R 727] ; [Fig. folio g iii : trois pendus.] Epitaphe dudit Villon. [Cote : Res Ye 245/Microfilm R 727]