Imunofenotipagem de lesões obtidas em carcinogênese quimicamente induzida por DMBA em glândulas salivares submandibulares e ratos (Rattus norvegicus)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Ultrastructural evaluation of the radioprotective effect of sodium selenite on submandibular glands in rats

The aim of this study was to evaluate the radioprotector effect of sodium selenite on the ultrastructure of submandibular glands in rats. Fifty-seven male albino Wistar rats were randomized to 4 groups: control, irradiated, sodium selenite and irradiated/sodium selenite. The animals in the sodium selenite and irradiated/sodium selenite groups received intraperitoneal injections of sodium selenite (0.5 mg/kg body weight) 24 h before irradiation. The animals belonging to the irradiated and irradiated/sodium selenite groups were submitted to 15 Gy of gamma radiation in the head and neck region. The submandibular glands were removed at 4, 8, 12, 24, 48 and 72 h after irradiation. The ionizing radiation induced damage to the secretory cells, especially the serous cells, right from the first period. Vacuolization, lysis of cytoplasmic inclusions and nuclear alterations occurred. The sodium selenite group also presented cellular alterations in the study periods, but with less damage compared to that caused by radiation. There was greater similarity between the irradiated/sodium selenite group and the control group than with the other groups treated in all study periods. Despite the alterations observed in the sodium selenite group, sodium selenite presented a radioprotective action on the secretory cells of submandibular glands.

Latent heat loss and sweat gland histology of male goats in an equatorial semi-arid environment

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Indole-3-carbinol attenuates the deleterious gestational effects of bisphenol A exposure on the prostate gland of male F1 rats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Labral gland in soldiers of the neotropical termite Cornitermes cumulans (Isoptera: Termitidae: Syntermitinae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Minimally Invasive Selective Neck Dissection: A Prospective Study of Endoscopically Assisted Dissection via a Small Submandibular Approach in cT(1-2)N(0) Oral Squamous Cell Carcinoma

Selective neck dissection (SND) in clinical N-0 (cN(0)) cases of oral squamous cell carcinoma (SCC) has been performed by surgeons using a retroauricular or modified facelift approach with robotic or endoscopic assistance. However, these procedures provide cosmetic satisfaction at the cost of possible maximal invasiveness. In this prospective study, we introduced and evaluated the feasibility as well as surgical invasiveness and cosmetic outcome of endoscopically-assisted SND via a small submandibular approach.Forty-four patients with cT(1-2)N(0) oral SCC (OSCC) were randomly divided into two groups of endoscopically-assisted SND and conventional SND. Perioperative and postoperative outcomes of patients were evaluated, including the length of the incision, operating time for neck dissection, estimated blood loss during the operation, amount and duration of drainage, total hospitalization period, total number of lymph nodes retrieved, satisfaction scores based on the cosmetic results, perioperative local complications, shoulder syndrome, and follow-up information.The mean operation time in the endoscopically-assisted group (126.04 +/- A 12.67 min) was longer than that in the conventional group (75.67 +/- A 16.67 min). However, the mean length of the incision was 4.33 +/- A 0.76 cm in the endoscopically-assisted SND group, and the amount and duration of drainage, total hospital stay, postoperative shoulder pain score, and cosmetic outcomes were superior in the endoscopically-assisted SND group. Additionally, the retrieved lymph nodes and complications were comparable.Endoscopically-assisted SND via a small submandibular approach had a longer operation time than the conventional approach. However, endoscopically-assisted SND was feasible and reliable while providing minimal invasiveness and satisfactory appearance.

Use of surgery and carboplatin in feline malignant mammary gland neoplasms with advanced clinical staging

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Collaboration of ultrasonography in the diagnosis of thyroid gland carcinoma in a dog - case report

The sonographic evaluation of thyroid glands in veterinary medicine presents challenges due to the complexity of the anatomical region, professional experience and type of ultrasonography equipment. The technique is considered a versatile diagnostic method that is noninvasive and has a low cost indicated in different clinical situations. Thyroid carcinoma is a malignant tumor that is often invasive and frequently metastatic to regional lymph nodes and lungs. The prognostic indicators for survival after surgery include tumor size, histological type, mobility and presence or absence of metastasis. The objective of the present report is to demonstrate the importance of ultrasound as a complementary method in the evaluation of thyroid carcinoma in dogs. At the Dr. Halim Atique Veterinary Hospital, an eight-year-old male Pit Bull was examined due to a history of firm painless swelling, approximately six inches in diameter, in the ventral cervical region, for about two months. The sonogram showed a nodular area, with defined and regular margins, and heterogeneous hypoechoic parenchyma, with areas of cavitation and swelling of the thyroid. Histopathology of the nodule was consistent with carcinoma. After thyreoidectomy and hormone replacemet, the patient is in good clinical condition.

Cytochemical study of Rhodnius neglectus and Rhodnius prolixus salivary gland cells (Hemiptera, Triatominae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Female paraurethral prostate gland in Bitches

Even though the presence of the female prostate has been reported in many species, including humans, bats and several rodents, it has many anatomical and histological variations. There is still plentiful discussion on the biological function of this organ. Many authors state that paraurethral ducts and glands are functional and homologous to the male prostate. The use of experimental models and a better knowledge of the female prostate gland in other species, can be useful to veterinary medicine as well as human medicine. Therefore the aim of this study is to check for the presence of this gland in female dogs of various breeds and age. For that purpose 25 urethras, from the bladder to the vulva, were collected, fixed in 10% neutral buffered formalin, routinely processed and sectioned into 4 slides of 4 µm, each with 40 µm gap between each set of 4 slides, using an automatic microtome and stained with Hematoxylin and Eosine (HE). The HE sections were evaluated for the presence of prostatic gland in the sample. Unstained tissue sections cut from paraffin blocks were marked with a polyclonal anti-PSA primary antibody. The prevalence of the gland was 32% (8/25). The structure of the paraurethral PSA-positive gland was acinar, organized in buds, with secretory epithelium varying from cubic to columnar; eccentric nuclei, with lose chromatin and a layer of basal cells, very similar to the male prostate were observed. In view of these characteristics, for the first time in the literature, was demonstrate that those glands. may be considered as female prostate in dogs, as they are in other vertebrates

Morphology of Richards' gland in the swarm-founding wasp (Hymenoptera, Vespidae)

Richards' gland is known for the majority of Epiponini wasps, and despite few experimental evidences, the taxonomic distribution in swarm-founder species and the function of this gland remain rather unclear. This work presents a morphological description of Richards' gland in Protonectarina sylveirae. The gland is formed by a cluster of class 3 cells underneath the anterior margin of the fifth metasomal sternite, and a reservoir formed by the intersegmental membrane between the fourth and fifth metasomal sternites where the secretion can be stored. The secretory cells contain a branched end apparatus that carries the secretory products towards the duct cell. Externally, the cuticle of the sternite, where the duct cells penetrate, is characterized by modifications as scales with very numerous pores. The presence of Richards' gland according to the model proposed by Samacá et al. 2013 in Protonectarina corroborates the single origin of this gland in Epiponini. The occurrence of a Golgi apparatus and smooth endoplasmic reticulum suggests pheromone production.

A simple high-performance liquid chromatography assay for on-line determination of catecholamines in adrenal gland by direct injection on an ISRP column

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Genistein reduces the noxious effects of in utero bisphenol A exposure on the rat prostate gland at weaning and in adulthood

Bisphenol A (BPA) is one hormonally active chemical with potential deleterious effects on reproductive organs, including breast and prostate. In contrast, genistein (GEN) is the major phytoestrogen of soy that presents potential protective effects against hormone-dependent cancers, including that of the prostate. Thus, pregnant Sprague-Dawley rats were treated with BPA at 25 or 250 μg/kg/day by gavage from gestational day (GD) 10-21 with or without dietary GEN at 250 mg/kg/chow (∼5.5 mg/kg/day). Then, male offspring from different litters were euthanized on post-natal day (PND) 21 and 180. At PND21, BPA 25 exposure induced early prostatic changes while dietary GEN attenuated some deleterious actions this xenoestrogen on epithelial cell proliferation levels, androgen receptor expression and prostatic architecture in male offspring. At PND180, a significant increase in incidence of prostatic multifocal inflammation/reactive hyperplasia and atypical hyperplasia were observed in male offspring from dams that received BPA 25. On the other hand, maternal GEN feeding attenuated some the adverse effects of BPA 25 on prostate disease at late-in-life. This way, the present findings point to preventive action of dietary GEN on deleterious effects of gestational BPA exposure in both early and late prostate development in offspring F1.

The long-term administration of calcineurin inhibitors decreases antioxidant enzyme activity in the rat parotid and submandibular salivary glands

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)