Follicular Development in Ewes During Natural and Prostaglandins Induced Estrous Cycle

The follicular development was evaluated in ovine females during natural and prostaglandin-F(2 alpha) (PG) induced estrous cycle. Ewes were randomly divided in two treatments (n=7/treatment): T1 with natural cycle and T2 synchronized with two injections of PG. From one day before PG injection until next ovulation, daily transrectal ultrasonography was done. All follicles >= 2 mm were assessed. During the interovulatory intervals, follicular growth and regression occurred in a wave like pattern (2-3 waves). The maximum diameter of the largest follicle of the first wave was greater in T1 (5.83 +/- 0.31 mm) compared with T2 (5.0 +/- 0.1 mm; P<0.01), but there was no significant difference among the emergency day of largest follicle, during the growth phase of the follicular waves. The duration of the plateau phase in wave 2 differed between the two treatments (P<0.05) showing 0.83 +/- 0.31 and 1.83 +/- 0.17 d, for natural and synchronized treatment, respectively. Growth rate did not differ between treatments. Presence of new luteal tissue was detected on day 3 after ovulation. In conclusion, the follicular development was similar in female ovine during natural and PG induced estrous cycle.

Competition and phylogeny determine community structure in Mullerian co-mimics

Until recently, the study of negative and antagonistic interactions (for example, competition and predation) has dominated our understanding of community structure, maintenance and assembly(1). Nevertheless, a recent theoretical model suggests that positive interactions (for example, mutualisms) may counterbalance competition, facilitating long-term coexistence even among ecologically undifferentiated species(2). Mullerian mimics are mutualists that share the costs of predator education(3) and are therefore ideally suited for the investigation of positive and negative interactions in community dynamics. The sole empirical test of this model in a Mullerian mimetic community supports the prediction that positive interactions outweigh the negative effects of spatial overlap(4) (without quantifying resource acquisition). Understanding the role of trophic niche partitioning in facilitating the evolution and stability of Mullerian mimetic communities is now of critical importance, but has yet to be formally investigated. Here we show that resource partitioning and phylogeny determine community structure and outweigh the positive effects of Mullerian mimicry in a species-rich group of neotropical catfishes. From multiple, independent reproductively isolated allopatric communities displaying convergently evolved colour patterns, 92% consist of species that do not compete for resources. Significant differences in phylogenetically conserved traits (snout morphology and body size) were consistently linked to trait-specific resource acquisition. Thus, we report the first evidence, to our knowledge, that competition for trophic resources and phylogeny are pivotal factors in the stable evolution of Mullerian mimicry rings. More generally, our work demonstrates that competition for resources is likely to have a dominant role in the structuring of communities that are simultaneously subject to the effects of both positive and negative interactions.

Morphological features and occurrence of degenerative characteristics in the hypopharyngeal glands of the paper wasp Polistes versicolor (Olivier) (Hymenoptera : Vespidae)

Different histochemical techniques were applied to examine the morphological features of the secretory cells of hypopharyngeal glands in the wasp Polistes versicolor. The results showed that most analyzed individuals present active glands with secretion stored in the cytoplasm. In some glands, morphological analyses revealed the presence of degenerative characteristics. Analyses of cellular integrity, however, did not detect dead cells. The results showed that, in P. versicolor, the development and regression of the hypopharyngeal glands were not age related, unlike glands of social bees. (c) 2006 Elsevier Ltd. All rights reserved.

Breeding activity patterns, reproductive modes, and habitat use by anurans (Amphibia) in a seasonal environment in the Pantanal, Brazil

Annual patterns of breeding activity, reproductive modes, and habitat use are described for a frog community in a seasonal environment, in the southern Pantanal, Mato Grosso do Sul, Brazil. Data were collected monthly between January 1995 and December 1998. A total of 24 species from four families; Bufonidae (3 species), Hylidae (10 species), Leptodactylidae (9 species), and Microhylidae (2 species) were registered. Three reproductive activity patterns are recognized among these species: continuous, explosive, and prolonged; 50% of the species were explosive breeders. Seasonal pattern of reproduction was verified for three analyzed years (1995-1997) most species reproduced during the rainy season (Nov-Jan). The reproduction was aseasonal in 1998; unexpected rains in the dry season lead to an unusual breeding activity. Five reproductive modes were noted - 62.5% of the species have the generalized aquatic mode, and 33.3% deposit eggs embedded in foam nests. Many species used the same sites for reproduction, although temporal partitioning and calling site segregation was observed. The occurrence of many species that exhibit explosive breeding early in the rainy season is common in seasonal and open environments with variable and unpredictable rainfall, as is the case in the Pantanal.

Molecular characterization and relatedness of Haematobia irritans (horn fly) populations, by RAPD-PCR

Haematobia irritans is a hematophagous parasite of cattle that causes significant economic losses in many parts of the world, including Brazil. In the present work, one American and four Brazilian populations of this species were studied by Random Amplified Polymorpht DNA (RAPD) to assess basically genetic variability within and between populations. Ten different decamer random primers were employed in the genomic DNA amplification, yielding 117 fragments in the five H.. irritans populations. In Drosophila prosaltans, used as an outgroup, 81 fragments were produced. Forty-three of these fragments were shared by both species. Among the H. irritans samples, that from Rio Branco (Acre State, Brazil) produced the smallest numbers of fragments and polymorphic bands. This high genetic homogenity may be ascribed to its geographic origin (in the Northwest of Brazil), which causes high isolation and low gene flow, unlike the other Brazilian populations, from the South Central region, in which cattle trade is very intensive. Marker fragments (exclusive bands) detected in every sample enabled the population origin to be characterized, but they are also potentially useful for further approaches such as the putative origin of Brazilian populations from North America. Similarity indices [Nei & Li, 1979, Proc. Natl. Acad. Sci. USA 76: 5269-5273] and phylogenetic trees, rooted by using the outgroup and produced by the Phylogenetic Analysis using Parsimony (PAUP 4.0-Swofford, 2001) program showed the closest relationships between flies from Sao Jose do Rio Preto and Turiuba (both from São Paulo State, Brazil) while flies from the geographically distant Rio Branco showed the greatest differentiation relative to the others.

Interceptação das chuvas em um fragmento de floresta da Mata Atlântica na Bacia do Prata, Recife, PE

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Chemical Composition and fermentative characteristics of Elephant grass (Pennisetum purpureum Schum.) silages added of increasing levels of mango (Mangifera indica L.) byproducts

Aiming at evaluating the use of those byproducts on ruminant feeding an experiment has been carried out in the Forage Research department (www.npf.ufc.br), in order to evaluate the effects of addition of increasing levels of mango processing byproducts (MB) in Elephant grass silages. Twenty experimental silos made of PVC pipe (100 x 340 mm), in a completely randomized design with 5 levels of addition (0; 5; 10; 15; and 20%) of MB and 4 replications. Upon 32 days of ensilage, samples were collected for determination of levels of dry Matter (DM), crude protein (CP), ethereal extract (EE), neutral detergent fiber (NDF), acid detergent fiber (ADF), hemicelluloses (HC), ammonia nitrogen (N-NH3), and pH values. Data were evaluated by analyses of variance and regression analyses using the F test at the 5% level. The levels of DM and CP increased of 0.50 and 0.03 percentage units, respectively, for every 1% of MB added. The levels of NDF and ADF decreased of 0.51 and 0.24 percentage units, respectively, for every 1% of MB added. The levels of N-NH3 decreased from 4 to 23 percentage units while pH values remained constant (p>0.05), with average values of 3.29. Addition of 20% of MB improved silages chemical composition by promoting an increase in the levels of MS and reduction in the levels of fiber.

An evaluation of the genetic diversity of Xylella fastidiosa isolated from diseased citrus and coffee in São Paulo, Brazil

Strains of Xylella fastidiosa, isolated from sweet orange trees (Citrus sinensis) and coffee trees (Coffea arabica) with symptoms of citrus variegated chlorosis and Requeima do Cafe, respectively, were indistinguish able based on repetitive extragenic palindromic polymerase chain reaction (PCR) and enterobacterial repetitive intergenic consensus PCR assays. These strains were also indistinguishable with a previously described PCR assay that distinguished the citrus strains from all other strains of Xylella fastidiosa. Because we were not able to document any genomic diversity in our collection of Xylella fastidiosa strains isolated from diseased citrus, the observed gradient of increasing disease severity from southern to northern regions of São Paulo State is unlikely due to the presence of significantly different strains of the pathogen in the different regions. When comparisons were made to reference strains of Xylella fastidiosa isolated from other hosts using these methods, four groups were consistently identified consistent with the hosts and regions from which the strains originated: citrus and coffee, grapevine and almond, mulberry, and elm, plum, and oak. Independent results from random amplified polymorphic DNA (RAPD) PCR assays were also consistent with these results; however, two of the primers tested in RAPD-PCR were able to distinguish the coffee and citrus strains. Sequence comparisons of a PCR product amplified from all strains of Xylella fastidiosa confirmed the presence of a CfoI polymorphism that can be used to distinguish the citrus strains from all others. The ability to distinguish Xylella fastidiosa strains from citrus and coffee with a PCR-based assay will be useful in epidemiological and etiological studies of this pathogen.

Validation of an immunoassay method for the determination of traces of carbaryl in vegetable and fruit extracts by liquid chromatography with photodiode array and mass spectrometric detection

A competitive enzyme-linked immunosorbent assay (ELISA) method for carbaryl quantitation in crop extracts was validated by liquid chromatography (LC) with diode array detection (DAD). For this purpose, six crops (banana, carrot, green bean, orange, peach and potato) were chosen for recovery and reproducibility studies. The general sample preparation included extraction with methanol followed by liquid-liquid partitioning and clean-up on Celite-charcoal adsorbent column of the vegetable extracts. ELISA samples consisted of a diluted LC extract in assay phosphate buffer (pH 7.5). The potential effect of methanol in these samples was evaluated. It was observed that a maximum content of 10% methanol present in the assay buffer could be tolerated without expressive losses in the ELISA performance. Under these conditions, a IC50 similar to 1.48 mu g l(-1) was obtained. A minimum matrix effect with a 1:50 dilution of the methanolic extracts in assay buffer was noticed, except for green bean samples that inhibited completely the assay. For the vegetable extracts, the ELISA sensitivities varied from 3.9 to 5.7 mu g l(-1), and good recoveries (82-96%) with R.S.D.s ranging from 5.7 to 12.1% were found. An excellent correlation between the LC-DAD and ELISA techniques was obtained. The confirmation of the carbaryl in less concentrated samples was achieved by LC-mass spectrometry interfaced with atmospheric pressure chemical ionisation. The [M + H](+)= 202 and [M + H-57](+)=145 ions, equivalent to the protonated molecular and l-naphthol ions, respectively, were used to carbaryl identification in these samples. (C) 1998 Elsevier B.V. B.V. All rights reserved.

Cloning, expression, purification and characterization of recombinant glutathione-S-transferase from Xylella fastidiosa

Xylella fastidiosa is an important pathogen bacterium transmitted by xylem-feedings leafhoppers that colonizes the xylem of plants and causes diseases on several important crops including citrus variegated chlorosis (CVC) in orange and lime trees. Glutathione-S-transferases (GST) form a group of multifunctional isoenzymes that catalyzes both glutathione (GSH)-dependent conjugation and reduction reactions involved in the cellular detoxification of xenobiotic and endobiotic compounds. GSTs are the major detoxification enzymes found in the intracellular space and mainly in the cytosol from prokaryotes to mammals, and may be involved in the regulation of stress-activated signals by suppressing apoptosis signal-regulating kinase 1. In this study, we describe the cloning of the glutathione-S-transferase from X. fastidiosa into pET-28a(+) vector, its expression in Escherichia coli, purification and initial structural characterization. The purification of recombinant xfGST (rxfGST) to near homogeneity was achieved using affinity chromatography and size-exclusion chromatography (SEC). SEC demonstrated that rxfGST is a homodimer in solution. The secondary and tertiary structures of recombinant protein were analyzed by circular dichroism and fluorescence spectroscopy, respectively. The enzyme was assayed for activity and the results taken together indicated that rxfGST is a stable molecule, correctly folded, and highly active. Several members of the GST family have been extensively studied. However, xfGST is part of a less-studied subfamily which yet has not been structurally and biochemically characterized. In addition, these studies should provide a useful basis for future studies and biotechnological approaches of rxfGST. (C) 2008 Elsevier B.V. All rights reserved.

HPLC determination of flumethrin, deltamethrin, cypermethrin, and cyhalothrin residues in the milk and blood of lactating dairy cows

A procedure to determine residue concentrations of synthetic pyrethroid insecticides (flumethrin, deltamethrin, cypermethrin and cyhalothrin) in the milk and blood of lactating dairy cows was developed. Extraction was performed with acetonitrile, n-hexane partitioning, and silica gel column cleanup with n-hexane and diethyl ether. Analysis was carried out by high- performance liquid chromatography and ultraviolet detection. Recovery of the four pyrethroids averaged 78 to 91% with a minimum detectable concentration of 0.001 mg/kg. The method was reproducible and sensitive.

The effect of trail length on task partitioning in three Acromyrmex species (Hymenoptera: Formicidae)

By comparing the behavior of three Acromyrmex (Hymenoptera, Formicidae) species during foraging on artificial trails of different lengths, we observed the occurrence of task partitioning and its relation to the food distance from the nest. Task partitioning was verified by leaf cache formation along the trail and leaf direct transferring among workers. There was significant difference between the number of leaf fragments carried directly to the fungus chamber and those transferred direct or indirectly, via cache, depending upon the trail length. Task partitioning could be a strategy used by leaf-cutting ants that allows the workers to use food sources far from their nests.

Evaluating genetic algorithms with different population structures on a lot sizing and scheduling problem

This paper studies the use of different population structures in a Genetic Algorithm (GA) applied to lot sizing and scheduling problems. The population approaches are divided into two types: single-population and multi-population. The first type has a non-structured single population. The multi-population type presents non-structured and structured populations organized in binary and ternary trees. Each population approach is tested on lot sizing and scheduling problems found in soft drink companies. These problems have two interdependent levels with decisions concerning raw material storage and soft drink bottling. The challenge is to simultaneously determine the lot sizing and scheduling of raw materials in tanks and products in lines. Computational results are reported allowing determining the better population structure for the set of problem instances evaluated. Copyright 2008 ACM.

The effects of soil pollution and its relation with morbidity in shoreline areas

Soil and subsoil pollution is not only significant in terms of environmental loss, but also a matter of environmental and public health. Solid, liquid and gaseous residues are the major soil contamination agents. They originate from urban conglomerates and industrial areas in which it is impossible to emphasize the chemical, petrochemical and textile industry; thermoelectric, mining, and ironmaster activities. The contamination process can thus be defined as a compound addition to soil, from what qualitative and or quantitative manners can modify soil's natural characteristics and use, producing baneful and deteriorative effects on human health. Studies have shown that human exposition to high concentration of some heavy metals found on soil can cause serious health problems, such as pulmonary or kidney complications, liver and nervous system harm, allergy, and the chronic exposition that leads to death. The present study searches for the correlation among soil contamination, done through a geochemical baseline survey of an industrial contamination area on the shoreline of Sao Paulo state. The study will be conducted by spatial analysis using Geographical Information Systems for mapping and regression analysis. The used data are 123 soil samples of percentage concentration of heavy metals. They were sampled and spatially distributed by geostatistics methods. To verify if there is a relation between heavy metals soil pollution and morbidity an executed correlation and regression analysis will be done using the pollution registers as the independent variables and morbidity as dependable variables. It is expected, by the end of the study, to identify the areas relation between heavy metals soil pollution and morbidity, moreover to be able to provide assistance in terms of new methodologies that could facilitate soil pollution control programs and public health planning. © 2010 WIT Press.

Economic growth in Latin America: the role of investment and other growth sources

Includes bibliography