SBD-RESP na busca ativa de casos de hanseníase

FUNDAMENTOS: A Sociedade Brasileira de Dermatologia Regional do Estado de São Paulo (SBD-RESP), apoiada pela Fundação Paulista Contra a Hanseníase, e em ação conjunta com os Serviços de Dermatologia do estado de São Paulo, credenciados pela Sociedade Brasileira de Dermatologia, lançou a campanha SBD-RESP na Busca Ativa de Casos de Hanseníase. OBJETIVOS: Auxiliar o Programa Nacional de Controle da Hanseníase no controle da doença. MÉTODO: Todos os Serviços de Dermatologia do estado de São Paulo, credenciados pela Sociedade Brasileira de Dermatologia, foram convidados e os 17 que participaram receberam uma planilha de dados e modelos de materiais informativos sobre a doença. A campanha foi realizada entre os meses de maio e julho de 2010. Ao término do período, cada serviço encaminhou a planilha de dados para análise estatística. RESULTADOS: Foram examinadas 1718 pessoas e diagnosticados, no total, 90 casos de hanseníase, a maioria do gênero masculino e da cor branca, com percentuais semelhantes de multibacilares e de paucibacilares. Doze por cento apresentavam história familiar de hanseníase. O maior número de casos detectados foi na capital, seguido, no interior, pela região de Presidente Prudente. O índice de detecção em menores de 15 anos foi 4%. CONCLUSÕES: Os resultados da campanha mostram a importância desta iniciativa da SBD-RESP. Sugere-se que ações semelhantes sejam repetidas e que se estendam a outras regionais da Sociedade Brasileira de Dermatologia

Isolamento de amebas de vida livre potencialmente patogênicas em poeira de hospitais

OBJETIVO: Observar a ocorrência de amebas de vida livre dos gêneros Acanthamoeba e Naegleria em amostras de poeira coletadas em hospitais. MÉTODOS: Foram coletadas 132 amostras de poeira em dois hospitais do município de Presidente Prudente, São Paulo. Os locais da coleta foram: Unidade de Terapia Intensiva, Centro Cirúrgico, Isolamento de Moléstias Infecciosas, Berçário, Emergência e Cozinha. As amostras foram semeadas em três meios de cultura: meio de ágar não nutriente com Escherichia coli, meio de ágar infusão de soja e microcultivo em meio de Pavlova modificado por Giazzi. As amebas isoladas foram identificadas segundo critérios morfológicos. RESULTADOS: O índice geral de positividade para amebas de vida livre, potencialmente patogênicas, dos gêneros Acanthamoeba e Naegleria, foi de 45,5%, sendo positivas 41,6% das amostras de poeira coletadas no hospital universitário e 50% no hospital estadual. Obtiveram-se 45,5% de positividade do gênero Acanthamoeba e 3,8% para amebas do gênero Naegleria. CONCLUSÕES: As amebas de vida livre, potencialmente patogênicas, estavam presentes em todos os ambientes estudados dos dois hospitais, sendo que as espécies do gênero Acanthamoeba foram as isoladas com maior freqüência.

AMERICAN VISCERAL LEISHMANIASIS: DISEASE CONTROL STRATEGIES IN DRACENA MICROREGION IN ALTA PAULISTA, SP, BRAZIL

Despite measures adopted to control American visceral leishmaniasis (AVL), the disease is spreading in a fast and worrying way throughout western São Paulo state. The aim of this work was to study the variables involved in the disease cycle as well as the effectiveness of controlling measures. The study was carried out in the microregion of Dracena, which is composed of twelve cities and belongs to Alta Paulista, a region of western São Paulo. The necessary data were provided by the Superintendence for Endemic Disease Control and Adolfo Lutz Institute, Regional Laboratory of Presidente Prudente. From August 2005 to January 2008, the following factors were observed: detection of phlebotomine sandflies in the cities and periods in which dogs or humans were diagnosed; number of human deaths; prevalence of suspected dogs tested by serology; percentage of euthanasia in suspected dogs; a possible correlation between positive dogs and cases of the disease in humans; and the disease prevalence among municipalities from the studied region. It was verified that, despite the strategies adopted in Dracena microregion to control AVL, the disease continues to rise. Thus, some procedures of the AVL Monitoring and Control

Qualidade tecnológica da cana-de-açúcar sob adubação com torta de filtro enriquecida com fosfato solúvel

The study had the objective to evaluate the effect of the fertilization with filter cake enriched with soluble phosphate on the sugar yield. The experiment carried at Presidente Prudente-SP, used a randomized complete block design, in the factorial scheme 5 x 4, where the first factor consisted of doses of filter cake (0; 0.5; 1.0; 2.0 and 4.0 t ha(-1)) and the second, doses of phosphorus fertilizer (0, 50, 100, 200 kg ha(-1) of P2O5), with 4 replicates, totaling 80 plots. The results indicated that phosphorus applied in planting furrows improves the quality of sugarcane raw matter by increasing the levels of soluble solids, total reducing sugars and sucrose in the stalks. The phosphorus also increases the productivity of sugar. The filter cake applied in planting furrow has the potential to partially replace the chemical fertilization with phosphate aiming to improve the quality and the productivity of sugar. The best combination was filter cake at dose between 2.6 and 2.7 t ha(-1) combined with dose between 160 and 190 kg P2O5 ha(-1) for obtaining best response of soluble solids and productivity of sugar.

Infestación natural de Lonchaeidae (Diptera) en variedades de melocotón

Se estudió la ocurrencia de Lonchaeidae en variedades de melocotón, conducidos sobre los patrones ‘Okinawa’ y Umê: Tropical, Ouromel 3, Jóia 4, Régis, Talismã, Aurora 2, Aurora 1, Dourado 2 y Doçura 2. Se colectaron muestras de 30 frutos por planta en el Banco de Germoplasma en Presidente Prudente, Brasil. Se obtuvieron 633 especímenes de Lonchaeidae; 394 de ellos en frutos de variedades sobre ‘Okinawa’ y 239 sobre Umê. Se capturaron especies como Neosilba zadolicha, N. inesperata, N. pendula, N. certa y Neosilba spp. (hembras). N. zadolicha y N. inesperata, se observaron en 77,78% de las muestras de las variedades sobre ‘Okinawa’. Sobre el patrón Umê la mayor incidencia fue de N. zadolicha , N. inesperata y N. pendula (55,6%, 33,3% y 33,3% respectivamente). Las variedades Ouromel 3, Talismã, Doçura 2 y Aurora 2 presentaron mayores infestaciones por Lonchaeidae. N. certa tuvo una menor incidencia y sólo se observó en la variedad Doçura 2 sobre ‘Okinawa’. Plantas con mayor número de frutos presentaron mayor incidencia de moscas por fruto; sin embargo, no hubo correlación entre peso del fruto y número de moscas. No se observó diferencia para peso del fruto y número de moscas por fruto entre los dos patrones, ‘Okinawa’ y Umê. Melocotones crecidos en los patrones ‘Okinawa’ y Umê en Presidente Prudente están infestados por especies de Lonchaeidae que presentan preferencia independientemente del peso de los frutos. El aporte de este trabajo al conocimiento de la comunidad de especies de Lonchaeidae ofrece una base para su control.

Water quality monitoring by nanostructured films in a sensing unit system

Presented here is a study about the capability of a sensing unit to detect changes in river water quality. In order to determine its accuracy, water quality was monitored at 11 points along the Veado River in Presidente Prudente, Brazil. To have a basis for comparison, a water quality index (WQI) was developed following methods previously applied in different watersheds. Results showed an accurate relationship between WQI and electric impedance readings detected by the sensing unit. Principal components analysis (PCA) was used to derive results in a form that can be correlated with WQI calculated for each sample point, which showed the potential application of this device.

Estudo da influencia do relevo nos dados de reflectancia da superficie do terreno atraves de imagens Landsat.

Presents a study of the spectral response of a specific vegetative cover under the same soil elevation angle, but in different classes of slope, through Landsat transparencies. The site located in the region of Presidente Prudente was studied through topo sheets to define the classes of slope. Densitometric readings were obtained of selected areas, representing the terrain reflectance in different relief conditions. The cluster analysis was used to classify the densitometric data according to the classes of slope. The map of classes of slope/reflectance of the terrain surface showed a high correlation, mainly for the classes A (0-10%) and B (10-20%). -from English summary

The Bauru Group: A continental Cretaceous unit in Brazil - Concepts based on micropaleontological, oxygen isotope and stratigraphical data

The integration of outcrop and subsurface information, including micropaleontological data, facies and sequence stratigraphic studies, and oxygen isotope analysis, allow us to present a new stratigraphic model for the Cretaceous continental deposits of the Bauru Group, Brazil. Thirty-eight fossil taxa were recovered from these deposits, including 29 species of ostracodes and 9 species of charophytes. Seven of these ostracode species and three subspecies are new and formally described here. The associations of Chara barbosai - Ilyocypris cf. riograndensis, found in the Adamantina Formation, and Amblyochara sp. - Neuquenocypris minor mineira nov. subsp., found in the Marília Formation. Ponte Alta Member, represent two distinct groups that are respectively Turonian-Santonian and Maastrichtian (probably Late Maastrichtian) in age. Therefore, a hiatus, encompassing more than 11 Ma, separates those two formations. From bottom to top, four depositional cycles were recognized in the Bauru Group in western São Paulo: cycles 1 and 2 belong to Caiuá Formation (fluvio-lacustrine and lacustrine deposits in the Presidente Prudente region), cycle 3 to the Santo Anastácio and lower Adamantina Formation (respectively fluvial and lacustrine deposits), and cycle 4 to the upper Adamantina Formation (fluvio-lacustrine facies). An erosional unconformity separates the Caiuá and Santo Anastácio Formations (between cycles 2 and 3). The Marília Formation is a distinct unit from the underlying succession; it does not occur in western São Paulo, but is found in restricted areas of São Paulo, Minas Gerais, Mato Grosso do Sul and Goiás States. During the deposition of the Bauru Group (Aptian? to Maastrichtian) the climate was hot and arid-semiarid. Shallow lakes underwent fluctuations in expansion (wet phases) and contraction (dry phases), as well as variations in salinity. During the deposition of the Adamantina Formation (Turonian-Santonian) there were long, dry periods that caused segmentation of large lakes (due to topographic irregularities in the basaltic substrate) and sometimes exposures of the lake floors; when flooded these lake floors were colonized by extensive meadows of single species of charophytes. Small ephemeral ponds, that were hydrochemically unstable and colonized by multiple species of charophytes, were the depositional sites for the marls and mudstones of Ponte Alta Member (Maastrichtian, Late Maastrichtian?). Our micropaleontological age control, combined with the Late Cretaceous ages of volcanic ashes found in the southeastern Brazil coastal basins, and the stratigraphic position of analcimites from the Jaboticabal-SP region, suggest a Late Coniacian-Santonian age for important magmatic events occurred in the interior of Brazil (north-central São Paulo State, Triângulo Mineiro, and southwestern Goiás State).

Aplicação do Índice Relaçáo Declividade-Extensão - RDE Na Bacia do Rio do Peixe (SP) para Detecção de Deformações Neotectô nicas

This paper presents the identification of tectonic deformation through the application of the stream-gradient index (slope x length) method in the Rio do Peixe hydrographic basin, located in western State of Sã o Paulo. The main principle used is that river valleys are the best tools for this type of analysis because of their fast adjustment to even the most gentle crustal deformations. This type of analysis for structural studies is extremely useful in the western region of the State of São Paulo, where deep chemical weathering results in a thick regolith and scarce outcrops. The stream length index is the ratio of the altimetric amplitude of each drainage and the natural logarithm of its length. The value obtained is plotted on maps at the median point of each drainage, allowing the drawings of lines with the same values (isodefs). Three anomalies (A, B and C) were identified along the Rio do Peixe valley representing uplift sectors indicated by the formation and distribution of Upper Quaternary deposits in terraces and modern fluvial plains and alluvial fans. In correlation with known tectonic data for this region it is possible to attribute the A and B anomalies to the Ribeirão Preto and Presidente Prudente crustal sutures, respectively. The smaller C anomaly needs further field investigation, but it could be associated with the Três Lagoas crustal suture. It must be emphasised that these sutures are inherited from the Precambrian Basement which had a strong influence on the Phanerozoic evolution in the whole sedimentary and volcanic stratigraphic pile of the Paraná Basin and on the dominant structural lineaments directions developed therein .The A, Band C anomalies are suggestive of renewed uplift as a result of neotectonic action in modern times.

Identificação de Enterobacter spp. através de técnicas convencionais e do sistema semi-automatizado Microscan (Autoscan-4)

A hundred and ninety-five (195) strains of Enterobacter spp., isolated from diverse clinical specimens - urine, feces, cateter, blood, wound, tracheal aspirate, vaginal fluid - were submitted to the conventional identification by biochemical tests, and were also submitted to the identification by panels NegCombo 20 of the system automated MicroScan - AutoScan- 4 (Dade Behring Inc., West Sacramento, CA, USA). The samples were from patients of the Clinical Laboratory from the School of Pharmacy and Biochemistry of UNOESTE, Presidente Prudente, SP, and from patients hospitalized at the University Hospital Domingos Leonardo Cerávolo, UNOESTE. Of the total of strains tested, 191 (97.9%) presented agreement between the two approaches utilized and 4 strains (2.1%) presented identification disagreement, that is, the genus identified was different in each approach. By this study, the conclusion is that both the approaches utilized for the identification presented advantages and disadvantages related to the cost, facility of execution, quickness, reliability and some other characteristics. Even so, our results showed that conventional methods represent a reliable tool for Enterobacter identification.

Detecção de prováveis deformações neotectônicas no Vale do Rio do Peixe, Região Ocidental Paulista, mediante aplicaçao de Índices RDE (Relac̈ão Declividade-Extensão) em segmentos de drenagem

Running water is one of the most important of all the physical processes which fashion the landscape, allowing gravity to operate along the valley floors. Besides this, the streams show a fast adjustment to the crustal deformations, even to the most gentle ones. This geologic behavior turns them a potential tool for neotectonic studies, specially the analysis of morphotnetric parameters associated with hydraulic gradient and discharge, this second factor being directly proportional to the extension of the streams. Both elements, gradient and stream length, can be combined in the SL index. The purpose of this paper is to show the RDE index application in the neotectonics analysis of the Rio do Peixe hydrographic basin and to compare the obtained values with the geologic basement incised by the streams. This basement encompasses Cretaceous sedimentary rocks of post-Serra Oeral Formation magmatism (Caiuá and Bauru groups) and Quaternary deposits that include chiefly recent alluvial plains and some Pleistocene terrace deposits. In the final part of this paper, an attempt is made in order to correlate the RDE results and the neotectonic framework admitted to this portion of the São Paulo State territory, as well as with field geologic, seismologic and paleoseismologic known elements. The results indicate the presence of two groups of anomalies: The first set corresponds to the Marília-Exaporã Plateau border, and the second one, located in the central portion of the hydrographic basin, is correlated to the Presidente Prudente seimogenic zone.

Análise da incerteza na representação de classes de cobertura do solo urbano resultantes da aplicação de uma rede neural artificial

The great diversity of materials that characterizes the urban environment determines a structure of mixed classes in a classification of multiespectral images. In that sense, it is important to define an appropriate classification system using a non parametric classifier, that allows incorporating non spectral (such as texture) data to the process. They also allow analyzing the uncertainty associated to each class from the output alues of the network calculated in relation to each class. Considering these properties, an experiment was carried out. This experiment consisted in the application of an Artificial Neural Network aiming at the classification of the urban land cover of Presidente Prudente and the analysis of the uncertainty in the representation of the mapped thematic classes. The results showed that it is possible to discriminate the variations in the urban land cover through the application of an Artificial Neural Network. It was also possible to visualize the spatial variation of the uncertainty in the attribution of classes of urban land cover from the generated representations. The class characterized by a defined pattern as intermediary related to the impermeability of the urban soil presented larger ambiguity degree and, therefore, larger mixture.

Multiplex PCR use for Staphylococcus aureus identification and oxacillin and mupirocin resistance evaluation

Oxacillin-resistant Staphylococcus aureus represents a serious problem in hospitals worldwide, increasing infected patients' mortality and morbidity and raising treatment costs and internment time. In this study, the results of using the Multiplex PCR technique to amplify fragments of the genes femA (specific-species), mecA (oxacillin resistance) and ileS-2 (mupirocin resistance) were compared with those of tests conventionally used to identify S. aureus isolates and ascertain their resistance to drugs. Fifty S. aureus strains were isolated from patients receiving treatment at UNOESTE University Hospital in Presidente Prudente, SP, Brazil. The 686 bp fragment corresponding to the gene femA was amplified and detected in all the isolates. On the other hand, the 310 bp fragment corresponding to the mecA gene was amplified in 29 (58%) of the isolates. All of the isolates showed sensitivity to mupirocin in the agar diffusion test, which was corroborated by the lack of any amplicon of the 456 bp fragment corresponding to the ileS-2 gene, in the PCR bands. The conventional tests to identify S. aureus and detect resistance to oxacillin and mupirocin showed 100% agreement with the PCR Multiplex results. The use of techniques for rapid and accurate identification of bacteria and assessment of their resistance may be valuable in the control of infection by resistant strains, allowing the rapid isolation and treatment of an infected patient. However, the results demonstrate that traditional phenotypic tests are also reliable, though they take more time.

Proposição de rotina morfológica para detecção de malha viária em imagens orbitais

The outdating of cartographic products affects planning. It is important to propose methods to help detect changes in surface. Thus, the combined use of remote sensing image and techniques of digital image processing has contributed significantly to minimize such outdating. Mathematical morphology is an image processing technique which describes quantitatively geometric structures presented in the image and provides tools such as edge detectors and morphological filters. Previous studies have shown that the technique has potential on the detection of significant features. Thus, this paper proposes a routine of morphological operators to detect a road network. The test area corresponds to an excerpt Quickbird image and has as a feature of interest an avenue of the city of Presidente Prudente, SP. In the processing, the main morphological operators used were threshad, areaopen, binary and erosion. To estimate the accuracy with which the linear features were detected, it was done the analysis of linear correlation between vectors of the features detected and the corresponding topographical map of the region. The results showed that the mathematical morphology can be used in cartography, aiming to use them in conventional cartographic updating processes.

Produtividade de cana-de-açúcar sob adubação com torta de filtro enriquecida com fosfato solúvel

Phosphorus is considered an essential element for plants and it is found in small amounts in Brazilian soils. The filter cake residue, composed of a mixture of bagasse and decanting sludge, has high levels of organic matter, phosphorus and calcium. The phosphorus present in the filter cake is organic, and its release, as it happens to the nitrogen, occurs gradually by mineralization and by microorganisms attack in the soil. This study aimed to evaluate sugarcane vegetative growth and yield under fertilization with filter cake enriched with soluble phosphate. The experiment was carried out in Presidente Prudente, São Paulo State, Brazil, by using a randomized complete block design, in a 5x4 factorial scheme, where the first factor consisted of filter cake doses (0 t ha-1, 0.5 t ha-1, 1.0 t ha-1, 2.0 t ha-1, and 4.0 t ha-1) and the second of phosphorus fertilizer doses (0 kg ha-1, 50 kg ha -1, 100 kg ha-1, and 200 kg ha-1 of P 2O5), with 4 repetitions, totalizing 80 plots. The experiment evaluated the tiller number, at 30, 60, 90, and 120 days after planting, oBrix, and yield. The stalk yield and tillering were influenced by the filter cake rates applied to the soil. Filter cake doses and their combination with phosphate did not change the juice quality (Brix) at harvest.