Inhibition of Trypanosoma brucei glucose-6-phosphate dehydrogenase by human steroids and their effects on the viability of cultured parasites

Dehydroepiandrosterone ( DHEA) is known as an intermediate in the synthesis of mammalian steroids and a potent uncompetitive inhibitor of mammalian glucose-6-phosphate dehydrogenase (G6PDH), but not the enzyme from plants and lower eukaryotes. G6PDH catalyzes the first step of the pentose-phosphate pathway supplying cells with ribose 5-phosphate, a precursor of nucleic acid synthesis, and NADPH for biosynthetic processes and protection against oxidative stress. In this paper we demonstrate that also G6PDH of the protozoan parasite Trypanosoma brucei is uncompetitively inhibited by DHEA and epiandrosterone (EA), with K(i) values in the lower micromolar range. A viability assay confirmed the toxic effect of both steroids on cultured T. brucei bloodstream form cells. Additionally, RNAi mediated reduction of the G6PDH level in T. brucei bloodstream forms validated this enzyme as a drug target against Human African Trypanosomiasis. Together these findings show that inhibition of G6PDH by DHEA derivatives may lead to the development of a new class of anti-trypanosomatid compounds. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.

Cytological Characterization of YpsB, a Novel Component of the Bacillus subtilis Divisome

Cell division in bacteria is carried out by an elaborate molecular machine composed of more than a dozen proteins and known as the divisome. Here we describe the characterization of a new divisome protein in Bacillus subtilis called YpsB. Sequence comparisons and phylogentic analysis demonstrated that YpsB is a paralog of the division site selection protein DivIVA. YpsB is present in several gram-positive bacteria and likely originated from the duplication of a DivIVA-like gene in the last common ancestor of bacteria of the orders Bacillales and Lactobacillales. We used green fluorescent protein microscopy to determine that YpsB localizes to the divisome. Similarly to that for DivIVA, the recruitment of YpsB to the divisome requires late division proteins and occurs significantly after Z-ring formation. In contrast to DivIVA, however, YpsB is not retained at the newly formed cell poles after septation. Deletion analysis suggests that the N terminus of YpsB is required to target the protein to the divisome. The high similarity between the N termini of YpsB and DivIVA suggests that the same region is involved in the targeting of DivIVA. YpsB is not essential for septum formation and does not appear to play a role in septum positioning. However, a ypsB deletion has a synthetic effect when combined with a mutation in the cell division gene ftsA. Thus, we conclude that YpsB is a novel B. subtilis cell division protein whose function has diverged from that of its paralog DivIVA.

DC Motor Speed Control via Fuzzy / Pole Placement / PI Controller

This report presents a new way of control engineering. Dc motor speed controlled by three controllers PID, pole placement and Fuzzy controller and discusses the advantages and disadvantages of each controller for different conditions under loaded and unloaded scenarios using software Matlab. The brushless series wound Dc motor is very popular in industrial application and control systems because of the high torque density, high efficiency and small size. First suitable equations are developed for DC motor. PID controller is developed and tuned in order to get faster step response. The simulation results of PID controller provide very good results and the controller is further tuned in order to decrease its overshoot error which is common in PID controllers. Further it is purposed that in industrial environment these controllers are better than others controllers as PID controllers are easy to tuned and cheap. Pole placement controller is the best example of control engineering. An addition of integrator reduced the noise disturbances in pole placement controller and this makes it a good choice for industrial applications. The fuzzy controller is introduce with a DC chopper to make the DC motor speed control smooth and almost no steady state error is observed. Another advantage is achieved in fuzzy controller that the simulations of three different controllers are compared and concluded from the results that Fuzzy controller outperforms to PID controller in terms of steady state error and smooth step response. While Pole placement controller have no comparison in terms of controls because designer can change the step response according to nature of control systems, so this controller provide wide range of control over a system. Poles location change the step response in a sense that if poles are near to origin then step response of motor is fast. Finally a GUI of these three controllers are developed which allow the user to select any controller and change its parameters according to the situation.

Talangfabriken : Om organisation av kunskap och kreativitet i skivindustrin

The purpose of this dissertation is to describe, explain and understand how record companies identify and develop new music and new talent. The analysis is carried out on three levels: individual, organizational and sector level. In a record company, this task formally goes to A&R (Artist and Repertoire). This dissertation takes its point of departure in how the capacity for discovering new talent can be understood in terms of knowledge, creativity and competence and how this capacity is affected in the meeting between the record company and the industry. The theoretical framework of the dissertation spans two sociological fields: the sociology of organizations and the sociology of knowledge. While it takes its organizational starting point in the Knowledge Company Approach, it employs a practice-based approach to discuss knowledge. I argue that within the Knowledge company approach there are two contrasting ways to understand knowledge; a distinction is made between knowledge- and creativity-intensive enterprises. The results show that the record industry’s polarized structure can be seen as a result of the Knowledge Company’s typical problems. The A&R’s work is described as including two phases, one intuitive and one analytical. The intuitive assessment is direct, unconscious and without reflection. This ability has been described as "intuition" and "gut feeling". The analytical phase adds analysis and reflection based on knowledge. The results from the interviews with A&R’s reveal the limit of formal and explicit knowledge not only in the choice of music but also in the marketing strategies. The overarching picture is one in which record companies move in a space characterized by tension between dichotomous forces – art and commercialism, creativity and knowledge, culture and economy, chaos and order, but where opposite poles are not mutually exclusive but complementary.

Health and quality of care from older peoples' and formal caregivers' perspective

Aim: The overall aim of this thesis was to gain a deeper understanding of older people's view of health and care while dependent on community care. Furthermore to describe and compare formal caregivers' perceptions of quality of care, working conditions, competence, general health, and factors associated with quality of care from the caregivers' perspective. Method: Qualitative interviews were conducted with 19 older people in community care who were asked to describe what health and ill health((I), good and bad care meant for them (II). Data were analyzed using content analysis (I) and a phenomenological analysis (II). The formal caregivers; 70 nursing assistants (NAs) 163 enrolled nurses (ENs) and 198 registered nurses (RNs), answered a questionnaire consisting of five instruments: quality of care from the patient's perspective modified to formal caregivers, creative climate questionnaire, stress of conscience, health index, sense of coherence and items on education and competence (III). Statistical analyses were performed containing descriptive statistics, and comparisons between the occupational groups were made using Kruskal-Wallis ANOVA, Mann-Whitney U-test and Pearson's Chi-square test (III). Pearson's  product moment correlation analysis and multiple regression analysis were performed studying the associations between organizational climate, stress of conscience, competence, general health and sense of coherence with quality of care (IV). Results: The older people's health and well-being were related to their own ability to adapt to and compensate for their disabilities and was described as negative and positive poles of autonomy vs. dependence, togetherness vs. being an onlooker, security vs. insecurity and tranquility vs. disturbance (I).  The meaning of good care (II) was that the formal caregivers respected the older people as unique individuals, having the opportunity to live their lives as usual and receiving a safe and secure care. Good care could be experienced when the formal caregivers had adequate knowledge and competence in caring for older people, adequate time and continuity in the care organization (II). Formal caregivers reported higher perceived quality of care in the dimensions medical-technical competence and physical-technical conditions than in identity-oriented approach and socio-cultural atmosphere (III). In the organizational climate three of the dimensions were close to the value of a creative climate and in seven near a stagnant climate. The formal caregivers reported low rate of stress of conscience. The RNs reported to a higher degree than the NAs/ENs a need to gain more knowledge, but the NAs and the ENs more often received training during working hours. The RNs reported lower emotional well-being than the NAs/ENs (III). The formal caregivers' occupation, organizational climate and stress of conscience were associated with perceived quality of care (IV). Implications: The formal caregivers should have an awareness of the importance of kindness and respect, supporting the older people to retain control over their lives. The nursing managers should employ highly competent and adequate numbers of skilled formal caregivers, organize formal caregivers having round the clock continuity. Improvements of organizational climate and stress of conscience are of importance for good quality of care.

Mellan etnologi och modern sociologi : K. Rob. V. Wikman som medlare i Finland och Sverige

Between ethnology and sociology: K. Rob. V. Wikman as a mediator in Finland and Sweden K. Rob. V. Wikman, professor in sociology at the Åbo Akademi University in Turku, Finland, played a central role both within Finnish and Swedish sociology in the 1940s and 1950s. He was a student of Westermarck and thus his own research represented an ethnosociological tradition, which at that time was challenged by modern, American-influenced sociological ideas. The aim of this article is to discuss the adaptation of “modern sociology” and the drawing of boundaries in Nordic sociology after the Second World War by focusing on Wikman’s work in Finnish as well as Swedish sociology, especially the assessor assignments he was given, and by giving emphasis to some of those that served as border poles or border markers in this process. The comparative starting point gives us reason to discuss some nationally characterized similarities and differences that can be observed in the establishment process of modern sociology in Finland and Sweden.

Monitoring Surface Displacement of the Colby Green Retaining Pond Dams

The Colby Green is a campus expansion project which began in October of 2003. The construction would result in three new buildings, additional parking, and an elliptical 75,000-squarefoot green southeast of Mayflower Hill Drive. There were also plans for the construction of three run-off management and sediment ponds below the green, to manage flooding of the green. Three drains in the green transport water to the three retaining ponds which slowly disperse water into the surrounding environment. The ponds were created by constructing earthen dams around the drain outlets. The dams are composed of soil, cobbles, and boulders procured from the surrounding excavation site. Unfortunately, earthen dams are susceptible to many types of erosion which result in their failure. In this case the potential for clay and silt from the underlying Presumpscot Formation to mix with the soil in the earthen dams raised concerns with regards to frost action. In order to monitor the surface displacement of the dams I drove 92 poles into the ground in 8 straight lines across the faces of the dams in the fall of 2005. I returned to the sites during and after the spring thaw of 2006, to check for any signs of movement resulting from frost-heave, surface creep, or any other form of mass wasting. Fortunately, there was no recordable sign of movement in the stakes across any of the retaining ponds. The dams appear to be functioning as designed.

Sentidos do trabalho informacional corporativo: uma investigação sobre o impacto do conhecimento no trabalho contemporâneo

This study proposes and uses a multipolar qualitative methodology for the investigation of the meanings of corporate work under the impact of the technical-scientific knowledge. Based on the constructionist epistemology and on a heideggerian approach, this methodology made possible the description of such phenomenon under the Management perspective, but also under the Psychology, Sociology and Philosophy views. Study starts with empirical data, collected by interviews and in publications, and analyzes them based on conceptual contributions from the four referred poles. As a result, this investigation proposes reflexivity, impermanence and entanglement as meanings of the corporate work that culminate in the paradoxical work. It also demonstrates that the impact of knowledge occurs in a reflexive spiral of complexification, continuously affecting individuals and work processes; describes the impermanent character of the work ruled by time, by neophilia and by uncertainties that make precarious the very subjective experience of worker; and proposes that this work becomes entangled due to the way it is managed, and is an entangling work, for embarrassing the individual in its techniques, logics and dissonances. The paradoxical essence that emerges from this appreciation of corporate work is described on the following scopes: its reflexivity does not prevent its rationality to be fragile; its dynamism coexists with the difficulty to achieve substantive organizational changes; its entanglement compromises the possibilities of an apparently reachable well-being; its focus on present ends up to be emptied; and its purpose of individual sustenance becomes unsustainable due to hyperconsumption. But the conclusions of the study do no describe a definitive phenomenon. At the ending, it points out possibilities of living with the paradoxical work as well as possibilities of its transformation.

Clusters de pequenas e médias empresas na área de produtos regionais: uma estratégia alternativa de desenvolvimento industrial sustentado na Zona Franca de Manaus

Este trabalho mostra que o fomento de clusters na área de produtos regIOnaIS - particularmente compostos por empresas de micro, pequeno e médio porte - representa uma estratégia alternativa econômica futura para a Zona Franca de Manaus - ZFM e o resto da Amazônia Ocidental. Além dos fundamentos teóricos relacionados aos clusters e pólos de desenvolvimento, o estudo baseou-se também nas evidências empíricas de quatro empresas de Manaus que operam com produtos regionais amazônicos. A pesquisa detecta a formação inicial de um cluster na área de produtos regionais que necessita de uma interação maior entre todos os agentes locais, a fim de ser operacionalizado. Conclui que estratégias econômicas para exploração das potencialidades regionais podem levar ao desenvolvimento de clusters de indústrias baseada na biodiversidade da região que usará matéria-prima local e conhecimento. Desta maneira, as estratégias de desenvolvimento sustentado podem trazer de volta muitos beneficios à região Amazônica e contribuir para a diversificação da atual base industrial da ZFM.

A efetividade dos instrumentos de financiamento público da FINEP no desenvolvimento do APL eletroeletrônico de Santa Rita do Sapucaí

O objetivo principal do estudo foi compreender a efetividade dos instrumentos públicos de subsídios financeiros aplicados no APL Eletroeletrônico de Santa Rita do Sapucaí, MG, pela Financiadora de Estudos e Projetos (FINEP), focando a empresa motriz do APL, Linear - Equipamentos Eletrônicos S/A. No estudo foram identificados os principais modelos de desenvolvimento regional, porém foram enfatizados os modelos de Arranjos Produtivos Locais (APLs) e Pólos de Crescimento, pois são os que mais caracterizam a região estudada. Também foram descritos a Lei da Inovação Tecnológica (LlT), os principais incentivadores de APLs no Brasil, ou seja, os agentes financeiros, bem como os atuais mecanismos e instrumentos de financiamentos da FINEP e como os mesmos são disponibilizados. Baseado nas políticas públicas traçadas, tanto pelo Governo Federal, como pelo Ministério da Ciência e Tecnologia (MCT), ao qual a FINEP se vincula, pode se analisar, no contexto do APL Eletroeletrônico de Santa Rita do Sapucaí, na figura da empresa Linear, a efetividade desses instrumentos de financiamento público estabelecidos para o desenvolvimento do referido APL. Finalmente, como conclusão e em resposta à questão inicial levantada na pesquisa, pode se compreender se houve efetividade dos instrumentos de financiamento público da FINEP no desenvolvimento do APL Eletroeletrônico de Santa Rita do Sapucaí.

A busca do "caminho do meio" em educação: contribuição à pedagogia dialética

Este trabalho é uma tentativa de pensar o ato de educar a partir de uma concepção dialética, isto é, considerando os pólos de uma contradição como complementares, e não excludentes. Nos quatro primeiros capítulos são apresentadas unidades de contrários observadas, respectivamente, na filosofia oriental, na ciência moderna, na psicanálise e na filosofia da linguagem, destacando-se contribuições e questionamentos que essas ciências e saberes podem oferecer à educação. No quinto e último capítulo avaliam-se as principais correntes de pensamento que influenciaram a teoria e prática educacionais, no Brasil, nas últimas décadas. A partir desse contexto, confrontam-se com os referenciais teóricos trabalhados, algumas questões colocadas pela prática em escolas de 1º e 2º graus, especialmente no projeto de 5ª e 8ª séries da Escola Senador Correia (escola particular, no Rio de Janeiro). Assim, busca-se livrar de abordagens maniqueístas a consideração de oposições tais como: escola moderna x tradicional; liberdade x coerção; autonomia x dependência diretivismo x espontaneísmo; e transmissão x construção do conhecimento.

MCDA: análise de decisão multicritério como ferramenta de avaliação de instalações portuárias: o caso dos terminais de contêineres brasileiros

Este trabalho busca avaliar, com suporte da metodologia MCDA - análise de decisão multicritério, os terminais de contêineres brasileiros quanto a suas potencialidades como vetores de crescimento sustentado da economia, no médio e longo prazo, para priorização de investimentos públicos e privados. O trabalho se consubstancia em um levantamento bibliográfico do tema decisório, que lhe serve de base, seguido de um estudo do tema portuário, a fim de levantar os fatores que tornam viável o florescimento e desenvolvimento de um sítio portuário, além de buscar tendências do setor de contêineres no Brasil. Após estas etapas, foi desenvolvido uma modelagem para o problema de avaliação dos terminais, com ajuda do software Expert Choice. Os resultados obtidos apontam para uma alteração sensível de paradigma no panorama portuário nacional em um cenário futuro. Portos que hoje se localizam na parte superior da lista de movimentação de contêineres, à frente nas estatísticas, podem não ter para onde se expandir, enquanto outros, que se encontram menos pujantes, podem florescer nas próximas décadas, devido às características de cada sítio portuário. As mais relevantes foram selecionadas como critérios do modelo desenvolvido, são eles: águas abrigadas, retroáreas, acessos terrestres e marítimos, equacionamento de questões ambientais, localização estratégica, vocação regional, extensão de cais e áreas de expansão. Entre as conclusões deste estudo, pode-se citar: 1 - O Porto de Santos, tradicional líder do ranking nacional em movimentação de contêineres, deve se manter entre os primeiros, graças à sua proximidade com o principal centro econômico e industrial nacional, a região da grande São Paulo, embora esteja com sua capacidade perto do limite operacional, conta com áreas de expansão, como o projeto Barnabé-Bagres. 2 - Outro porto que se destacou na classificação final foi o de Itaguaí, já hoje com movimentação crescente e enorme potencial de crescimento na área de contêineres. Possui excelente condição de águas abrigadas, boa localização estratégica, entre Rio de Janeiro e São Paulo, dois pólos econômicos fortes, com influência decisiva no cenário nacional, e que dispõe de consistente plano de expansão, especialmente relacionado ao aumento de contêineres.

Impacto das tecnologias digitais nas bibliotecas universitárias: reflexões sobre o tema

MOREIRA, Luciana Moreira; SILVA, Armando Malheiro da. Impacto das tecnologias digitais nas bibliotecas universitarias: reflexões sobre o tema. Informaçao e sociedade: estudos. Joao Pessoa, v.19, n.3, p. 125-132,2009.

Democratização do acesso ao ensino superior: os cursos de graduação à distância na UFRN

This thesis pursuits to contextualize the theoretical debate between the implementation of public education policy of the Federal Government focused in a distance learning and legal foundations for its enforcement, in order to raise questions and comments on the topic in question. Its importance is back to provide scientific input and can offer to the academy, particularly in the UFRN, and elements of society to question and rethink the complex relationship between the socio-economic and geographic access to higher education. It consists of a descriptive study on the institutionalization of distance education in UFRN as a mechanism for expanding access to higher education, for both, the research seeks to understand if the distance undergraduate courses offered by the UAB system and implemented at UFRN, promote expanding access to higher education, as it is during implementation that the rules, routines and social processes are converted from intentions to action. The discussion of this study lasted between two opposing views of Implementation models: Top-down and Bottom-up. It is worth noting that the documents PNE, PDE and programs and UAB MEETING reflect positively in improving the educational level of the population of the country It is a qualitative study, using the means Bibliographic, Document and Field Study, where they were performed 04 (four) in 2010 interviews with the management framework SEDIS / UAB in UFRN. The data were analyzed and addressed through techniques: Document Analysis and Content Analysis. The results show that the process of implementation of distance education at UFRN is in progress. According to our results, the research objective is achieved, but there was a need to rethink the conditions of the infrastructure of poles, the structure of the academic calendar, the management of the SEDIS UFRN, regarding the expansion of existing vacancies and the supply of new courses by the need for a redesign as the Secretariat's ability to hold the offerings of undergraduate courses offered by the Federal Government to be implemented in the institution. It was also found that levels of evasion still presents a challenge to the teaching model. Given the context, we concluded that the greatest contribution of UAB and consequently UFRN by distance learning for undergraduate courses (Bachelor in Mathematics, Physics, Chemistry, Geography and Biological Sciences, beyond the bachelor's degrees in Business and Public Administration ) is related to increasing the number of vacancies and accessibility of a population that was previously deprived of access to university

De volta para os braços da rainha dos céus: migração, memória e festa em Caicó/RN

The present work has as an objective to seize up some particular meanings of the Feast of Sant Ana (The saint which represents the city of Caicó/RN), a central event of the calendar festive-religious of that city, looking for to perceive on how it combines with the symbolic poles sacred: profane through the social bonds that are present there performed and expressed through the social networking and of local belongings. Such bond ties reveal themselves through a very especial manner, through the migration of returning to the feast, when collectively are produced and updated knowledge and codes that reinforce the representation of the local belonging, nominated as being caicoense . We seek on perceiving how the many events of this feast (from a perspective of a religious order as well as laic) acting on a conclusive way to an identity of the re-meaning of that city, especially in its relation with the migrant natives: the sons and daughters that return to the arms of Sant Ana