Morphology and histochemistry of the digestive tract in carnivorous freshwater Hemisorubim platyrhynchos (Siluriformes: Pimelodidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Fator de condição e biologia alimentar de Pimelodus maculatus em três pontos do Rio Piracicaba (SP)

A number of 170 individuals of the species Pimelodus maculatus, a Neotropical fish known as mandi was collected in three distinct locations of the Piracicaba river (Piracicaba, Santa Maria da Serra and Americana – SP0 from April 2009 to February 2010. A common species of catfish whose individuals exist in many neotropical basins was observed to get more information about a possible influence of the water conditions in distant parts of a same river to its condition and also to verify whether the stomach contents of fish collected in these distinct places may distinguish as a possible consequence of food availability. The species were studied by collecting biological data such as standard length (SL) in cm, their total weights (TW ) in grams and sexes to see the composition of fish present in the samples. Length-weight relationships are important means to investigate facts related to the condition of the species in these systems. The analysis was made by using covariance to observe if individuals of similar lengths collected in distinct places may be compared to this purpose. Usual methods to investigate relationships of stomach food contents were applied. By analyzing the condition factor, one may conclude that the mandis collected in the region of Santa Maria da Serra presented similar characteristics to those obtained in Piracicaba, but they differ in this aspect for individuals collected in Americana. This fact is not conclusive about the reasons of this situation, but it may be a first step to other studies in order to get more information about. The degree of stomach repletion may be a first approach to this possible explanation, as individuals of Piracicaba presented usually full stomachs and differed in this way to individuals collected in the two other places

Autonomic control of post-air-breathing tachycardia in Clarias gariepinus

The African catfish (Clarias gariepinus) is a teleost with bimodal respiration that utilizes a paired suprabranchial chamber located in the gill cavity as an air-breathing organ. Like all air-breathing fishes studied to date, the African catfish exhibits pronounced changes in heart rate (f H) that are associated with air-breathing events. We acquired f H, gill-breathing frequency (f G) and air-breathing frequency (f AB) in situations that require or do not require air breathing (during normoxia and hypoxia), and we assessed the autonomic control of post-air-breathing tachycardia using an infusion of the β-adrenergic antagonist propranolol and the muscarinic cholinergic antagonist atropine. During normoxia, C. gariepinus presented low f AB (1.85 ± 0.73 AB h−1) and a constant f G (43.16 ± 1.74 breaths min−1). During non-critical hypoxia (PO2 = 60 mmHg), f AB in the African catfish increased to 5.42 ± 1.19 AB h−1 and f G decreased to 39.12 ± 1.58 breaths min−1. During critical hypoxia (PO2 = 20 mmHg), f AB increased to 7.4 ± 1.39 AB h−1 and f G decreased to 34.97 ± 1.78 breaths min−1. These results were expected for a facultative air breather. Each air breath (AB) was followed by a brief but significant tachycardia, which in the critical hypoxia trials, reached a maximum of 143 % of the pre-AB f H values of untreated animals. Pharmacological blockade allowed the calculation of cardiac autonomic tones, which showed that post-AB tachycardia is predominantly regulated by the parasympathetic subdivision of the autonomic nervous system.

Morphology and Histochemistry of the Liver of Carnivorous Fish Hemisorubim platyrhynchos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Chilling of Steindachneridionparahybae (siluriformes: pimelodidae) embryos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Morfologia e histoquímica do tubo digestivo de Mapará Hypophthalmus marginatus

Pós-graduação em Aquicultura - FCAV

Autonomic control of post-air-breathing tachycardia in Clarias gariepinus (Teleostei: Clariidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Fontes lipídicas e ácidos graxos essenciais no desenvolvimento ovariano e desempenho reprodutivo de jundiás Rhamdia quelen

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Experimental Bolbophorus damnificus (Digenea: Bolbophoridae) Infections in Piscivorous Birds

In order to determine potential definitive hosts of the digenetic trematode, Bolbophorus damnificus, two American White Pelicans (Pelecanus erythrorhynchos), two Double-crested Cormorants (Phalacrocorax auritus), two Great Blue Herons (Ardea herodias), and two Great Egrets (Ardea alba) were captured, treated with praziquantel, and fed channel catfish (Ictalurus punctatus) infected with B. damnificus metacercariae. Patent infections of B. damnificus, which developed in both American White Pelicans at 3 days post-infection, were confirmed by the presence of trematode ova in the feces. Mature B. damnificus trematodes were recovered from the intestines of both pelicans at 21 days post-infection, further confirming the establishment of infection. No evidence of B. damnificus infections was observed in the other bird species studied. This study provides further evidence that Double-crested Cormorants, Great Blue Herons, and Great Egrets do not serve as definitive hosts for B. damnificus.

Bird Depredation

A survey of catfish producers by the United States Department of Agriculture, Centers for Epidemiology and Animal Health (CEAH) in 1996 indicated that the two primary sources of catfish losses in commercial operations were disease (45%) and wildlife (37%) (CEAH 1997a). A variety of avian and mammalian predators are amracred to aquaculture facilities in the United States (Parkhurs: er al. 1992) because ponds and open raceways provide a constant and readily accessible food supply for these animals. However, the mere presence of these predators arcund aquaculture faciliries does not necessarily mean that significant depredation problems are occurring. At catfish farms, three species or species groups of birds are primarily cited by catfish producers as causing most depredation problems (Wywialowski 1999). These include doublecrested cormorants, wading birds (herons and egrets), and American white pelicans, in order of importance to catfish producers (Wywialowski 1993). Although all of these species consume catfish, their biology, distribution, dietary preferences dictare the extent of depredation problems they cause and the approaches needed to alleviate their depredations. With the exception of total bird exclusion from ponds, there are no simple solutions for resolving all bird depredation problems in catfish aquaculture. Thus, in most cases, an integrated management approach to alleviating bird depredations must be considered.

A NEW SPECIES OF CATHOROPS (SILURIFORMES; ARIIDAE) FROM MESOAMERICA, WITH REDESCRIPTION OF FOUR SPECIES FROM THE EASTERN PACIFIC

The great similarities in the external morphologies and the lack of knowledge on ontogenetic and intersexual differences of species in the ariid genus Cathorops Jordan and Gilbert, 1882, has led to an abundance of misidentifications, causing great nomenclatural instability. Accordingly, the taxonomic statuses of the Cathorops species described from Eastern Pacific have remained controversial in the literature, even in recent studies. Here, we describe Cathorops raredonae, a new species from Mesoamerica (Mexico to El Salvador) and redescribe (in Cathorops) Tachysurus liropus Bristol, 1897, and Arius taylori Hildebrand, 1925, often listed as junior synonyms of Cathorops fuerthii (Steindachner, 1877) and Cathorops steindachneri (Gilbert and Starks, 1904), respectively, or treated as species inquirendae in Cathorops. We also redescribe and redefine the circumscriptions of C. fuerthii and C. steindachneri. Finally, we summarize current statuses of nominal species of Cathorops from the Eastern Pacific and provide an artificial key to identify the valid Pacific species.

Two new species of Jupiaba (Characiformes: Characidae) from the rio Tapajos and rio Madeira drainages, Brazil, with an identification key to species of the genus

Two new species of Jupiaba Zanata are described from Brazil. Jupiaba iasy, new species, is described from rio Teles Pires and rio Jamanxim, tributaries of rio Tapajos, and from rio Aripuana, in the rio Madeira drainage. It is distinguished from its congeners by its color pattern consisting of a single posteriorly displaced dark crescent-shaped humeral blotch, situated over the first 5 to 7 lateral line scales, and an inconspicuous dark spot at the end of caudal peduncle. It also differs from all remaining Jupiaba for the following combination of characters: 34-36 lateral line scales, 19-21 branched anal-fin rays, 8-10 predorsal scales arranged in a regular row, 6 horizontal series of scales above and 4 series below lateral line, body depth 32.3-36.1% of SL, and absence of filamentous rays in the first dorsal and anal-fin rays. Jupiaba paranatinga, new species, is described from rio Teles Pires, tributary of rio Tapajos. It is distinguished by having 34-35 lateral line scales, two vertically elongated humeral blotches, a conspicuous caudal spot at the end of the caudal peduncle, extending over 8-10 median caudal-fin rays, eye diameter 43.7-46.9% of HL, and relatively low body depth (31.3-35.5% of SL). Additionally, comments on the putative relationships of the new species with their congeners and an updated key to the species of the genus are provided.

The cascudos of the genus Hypostomus Lacepede (Ostariophysi: Loricariidae) from the rio Iguacu basin

We reviewed several large collections of the genus Hypostomus from the rio Iguacu basin summing up to 793 specimens mainly from the Laboratorio de Ictiologia do Departamento de Ecologia e Biologia Evolutiva from Universidade Federal de Sao Carlos, from fish collection of Nucleo de Pesquisas em Limnologia, Ictiologia e Aquicultura da Universidade Estadual de Maringa, and from the Museu de Historia Natural do Capao da Imbuia. Hypostomus albopunctatus, H. commersoni, H. derbyi, and H. myersi are redescribed and Hypostomus nigropunctatus is described as a new species. A practical key for identification of Hypostomus species from the rio Iguacu is also provided.

Protein diets promote the maturation of oocytes and spawning of Piaractus mesopotamicus kept in cages

The objective of this study was to evaluate the effects of balanced diets on the maturation of oocytes and the reproductive performance of P. mesopotamicus in cages. A completely random design with 224 fish in 16 cages measuring 5 m(3) was employed for this purpose. The treatments consisted of diets containing 18, 24, 30, and 36% crude protein (CP) provided ad libitum. The external and internal morphological characteristics of the specimens were examined, as well as: the position of the germinal vesicle, the distribution of oocyte diameters, the fertilization and hatching rates, the number of oocytes released, the total number of oocytes, the remaining weight and total weight of the ovaries, the gonadosomatic index, the condition factor (K), and the histology of the oocytes and ovaries post-spawning and during ovarian regression. The diameters of the oocytes collected before the first hormonal application displayed a unimodal distribution for the lowest protein content and a polymodal distribution for the other treatments. A similar situation was seen during spawning. The lowest fertilization and hatching rates were found as a consequence of the treatment with 30% CP (P < 0.05). The greatest hatching rate occurred in the females fed 18% CP. The greatest total oocyte weight was found in the specimens that received between 30 and 36% CP. The lowest K index was found in the females fed 36% CP. In conclusion, a diet containing 18% CP satisfies the reproductive requirements of females adapted to this system.

Embryonic development and larval stages of Steindachneridion parahybae (Siluriformes: Pimelodidae) - implications for the conservation and rearing of this endangered Neotropical species

Steindachneridion parahybae is a freshwater catfish endemic to the Paraiba do Sul River and is classified as an endangered Neotropical species. An increasing number of conservation biologists are incorporating morphological and physiological research data to help conservation managers in rescue these endangered species. This study investigated the embryonic and larval development of S. parahybae in captivity, with emphasis in major events during the ontogeny of S. parahybae. Broodstocks were artificially induced to reproduce, and the extrusion occurred 200-255 degree-hours after hormonal induction at 24 degrees C. Larval ontogeny was evaluated every 10 minutes under microscopic/stereomicroscopic using fresh eggs samples. The main embryogenic development stages were identified: zygote, cleavage, including the morula, blastula, gastrula phase, organogenesis, and hatching. The extruded oocytes showed an average diameter of 1.10 +/- 0.10 mm, and after fertilization and hydration of eggs, the average diameter of eggs increased to about 1.90 +/- 0.60 mm, characterized by a large perivitelline space that persisted up to embryo development, the double chorion, and the poles (animal and vegetative). Cell division started about 2 minutes after fertilization (AF), resulting in 2, 4, 8 (4 x 2 arrangement of cells), 16 (4 x 4), 32 (4 x 8) and 64 (2 x 4 x 8) cells. Furthermore, the blastula and gastrula stages followed after these cells divisions. The closed blastopore occurred at 11 h 20 min AF; following the development, the organogenetic stages were identified and subdivided respectively in: early segmentation phase and late segmentation phase. In the early segmentation phase, there was the establishment of the embryonic axis, and it was possible to distinguish between the cephalic and caudal regions; somites, and the optic vesicles developed about 20 h AF. Total hatching occurred at 54 h AF, and the larvae average length was 4.30 +/- 0.70 mm. Gradual yolk sac reduction was observed during the first two days of larval development. The first feeding occurred at the end of the second day. During the larval phase, cannibalism, heterogeneous larval growth and photophobia were also observed. This information will be important in improving the artificial reproduction protocols of S. parahybae in controlled breeding programs.