An extracellular transglutaminase is required for apple pollen tube growth

An extracellular form of the calcium-dependent protein-cross-linking enzyme TGase (transglutaminase) was demonstrated to be involved in the apical growth of Malus domestica pollen tube. Apple pollen TGase and its substrates were co-localized within aggregates on the pollen tube surface, as determined by indirect immunofluorescence staining and the in situ cross-linking of fluorescently labelled substrates. TGase-specific inhibitors and an anti-TGase monoclonal antibody blocked pollen tube growth, whereas incorporation of a recombinant fluorescent mammalian TGase substrate (histidine-tagged green fluorescent protein: His6-Xpr-GFP) into the growing tube wall enhanced tube length and germination, consistent with a role of TGase as a modulator of cell wall building and strengthening. The secreted pollen TGase catalysed the cross-linking of both PAs (polyamines) into proteins (released by the pollen tube) and His6-Xpr-GFP into endogenous or exogenously added substrates. A similar distribution of TGase activity was observed in planta on pollen tubes germinating inside the style, consistent with a possible additional role for TGase in the interaction between the pollen tube and the style during fertilization.

Apple

Apple is a collection of poems that explores the connection between human relationships and the evolution of an identity. Multiple speakers investigate gender and sexuality, plentitude and poverty, atheism and Christianity in order to better understand some of the forces that affect a woman's consciousness. An awareness of perceived dualities, such as self and other, reason and faith, nature and technology, socialization and loneliness are central to this exploration. The poems employ various forms, such as ultra-talk narratives, lyrical meditations, prose poetry, epistolary poems and hypertext. The variety of structure and form in the collection mirrors the variety of approaches the speakers employ to move closer and further away from the subjects at hand. The rhetorical posture employed in each poem is directly linked to the speaker's relationship with the audience, which is an excellent example of a human relationship affecting the evolution of an identity.

Um estudo teórico sobre o valor da marca de empresas consideradas inovadoras

O objetivo central deste trabalho é descrever a trajetória do valor da marca de empresas de alta tecnologia, consideradas inovadoras. Como objetivo específico pretende-se identificar as prováveis causas da variação de valor de mercado e de posicionamento da marca. O trabalho apresenta as razões do sucesso, estagnação, declínio e queda de um grupo de empresas escolhido em razão do crescimento ou declínio brusco. Trata-se de uma pesquisa descritiva e bibliográfica. Para tanto, foram escolhidas quinze das maiores empresas de Tecnologia da Informação e Telecomunicações, segundo ranking de valor de marca elaborado pelas agências Interbrand e BrandZ. Observa-se que as empresas implantaram estratégias orientadas à inovação, resultando em altos investimentos em P&D, dispendidos nas duas últimas décadas. As pesquisas utilizadas permitiram associar este estudo da trajetória e das variações no valor da marca das empresas às estratégias e direcionamentos de mercado nos últimos vinte anos, tais como: a aquisição da Compaq e da EDS pela HP, a trajetória de recuperação da Apple, a concorrência entre a Apple e a Nokia, em valor de mercado, e a permanência da centenária IBM em um setor constantemente ameaçado por novos entrantes.

Apple Pollination: Demand Depends on Variety and Supply Depends on Pollinator Identity

Insect pollination underpins apple production but the extent to which different pollinator guilds supply this service, particularly across different apple varieties, is unknown. Such information is essential if appropriate orchard management practices are to be targeted and proportional to the potential benefits pollinator species may provide. Here we use a novel combination of pollinator effectiveness assays (floral visit effectiveness), orchard field surveys (flower visitation rate) and pollinator dependence manipulations (pollinator exclusion experiments) to quantify the supply of pollination services provided by four different pollinator guilds to the production of four commercial varieties of apple. We show that not all pollinators are equally effective at pollinating apples, with hoverflies being less effective than solitary bees and bumblebees, and the relative abundance of different pollinator guilds visiting apple flowers of different varieties varies significantly. Based on this, the taxa specific economic benefits to UK apple production have been established. The contribution of insect pollinators to the economic output in all varieties was estimated to be £92.1M across the UK, with contributions varying widely across taxa: solitary bees (£51.4M), honeybees (£21.4M), bumblebees (£18.6M) and hoverflies (£0.7M). This research highlights the differences in the economic benefits of four insect pollinator guilds to four major apple varieties in the UK. This information is essential to underpin appropriate investment in pollination services management and provides a model that can be used in other entomolophilous crops to improve our understanding of crop pollination ecology.

Nokia- The surprising success of textbook wisdom

What is the secret behind Nokia’s phenomenal success in mobile phones? More generally, how to succeed in a turbulent high technology market? Historical and comparative studies bring understanding not only about past phenomena, but also insights about current and coming ones. In this paper we compare Ford and General Motors in the 1920s and 1930s with Motorola and Nokia in the 1980s and 1990s. The insight from this comparison is how reinvention of the product, category, relative to the market incumbent’s market conceptualization, is a recipe for success. Nokia’s success in the 1990s was based on following the guidelines pioneered by General Motors in the 1920s: market segmentation, embracing the latest technological advances, and brand management in the pursuit of consumer repurchases. The article tells how Nokia addresses both GM’s legacy and changes in the business environment. At the end of our paper, we disuss the generalizability of our findings.

Comparison between benzyladenine and metamitron as chemical thinning agents in Gala, Kanzi, Pink Lady and Red Delicious apple cultivars

Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia - UL

Estudo in silico de sítios de restrição enzimática de genes da proteína capsidial e análise de diversidade genética de Apple chlorotic leaf spot virus em fruteiras de semente e caroço.

Cultivares comerciais de macieiras são infectadas por 3 espécies principais de vírus: Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASGV) e Apple stem pitting virus (ASPV), geralmente em infecções complexas. O objetivo do estudo foi caracterizar a diversidade genética de genes da proteína capsidial (CP) de isolados de ACLSV.

Advances on apple production under semiarid climate: N fertigation

The fertilizing management for apple tree is essential, especially for nitrogen, one of the most important nutrients affecting fruit yield. Thus, an experiment was conducted in 2012 and 2013 to evaluate the fruit production, yield and leaf chlorophyll of ?Princesa? and ?Eva? apples as a function of nitrogen fertigation under Brazilian semiarid conditions. The experimental design consisted of randomised blocks, with treatments distributed in a factorial arrangement 2 x 4, corresponding to apple cultivars (Eva and Princesa); and nitrogen doses (160, 120, 80 and 40 kg of N ha-1), with four replications and three plants. Calcium nitrate was used as nitrogen source (15.5% of N) with applications twice a week during 40 days, reaching 12 fertilizing performances through irrigation water. The following variables were evaluated: i) fruit production per plant (kg plant-1); ii) fruit yield (t ha-1); iii) number of fruits per plant; iv) leaf chlorophyll meter readings (index); and v) leaf nitrogen concentration (g kg-1). Princesa apple cultivar if compared to ?Eva? presents a better fruit production performance under Brazilian semiarid. Furthermore, nitrogen doses fertilized through irrigation water have no effect on fruit production of Eva and Princesa apple cultivars during the first production cycle.

Integrated control of two tortricid (Lepidoptera) pests in apple orchards with sex pheromones and insecticides.

The apple is attacked by a significant number of insect pests in Brazilian commercial orchards, including Bonagota salubricola and Grapholita molesta (Lepidoptera: Tortricidae). Sexual disruption of B. salubricola and G. molesta was evaluated in apple orchard using the flowable pheromone formulations, SPLAT Grafo+Bona (SG+B), SPLAT Attract and Kill Grafo+Bona (SAKG+B), and compared with the standard insecticides used for management in the Integrated Apple Production (IAP) system. Both formulations were applied at a rate of 1kg/ha on October 10, 2005 and December 13, 2005 using 300 and 1000 point sources/ha of SG+B and SAKG+B, respectively in experimental units of 7 ha. Adult male captures of B. salubricola and G. molesta were evaluated weekly in Delta traps with specific synthetic sex pheromone from October 10, 2005 to February 14, 2006. Damage to fruits was evaluated on November 21 and December 21, 2005, and January 25 and February 14, 2006. In the SPLAT treated experimental units a significant reduction was observed in the number of B. salubricola and G. molesta males caught in Delta traps compared to the experimental unit IAP. Damage by B. salubricola at harvest ranged from 1.63 to 4.75% with no differences between treatments, while damage by G. molesta was near zero in all experimental units. Mating disruption using SG+B and SAKG+B was sufficient to control B. salubricola and G. molesta with results equivalent to IAP guidelines. This technology is promising for management of both pests in Brazilian apple orchards with immediate reduction of 43% in the number of insecticide applications.

Occurrence of apple stem grooving capillovirus in Santa Catarina, Brazil, detected by RT-PCR.

1999

The effect of different training systems on vegetative and productive performance of apple trees cultivar 'maxigala' - in southern Brazil.

2016

Development and productivity of apple tree cv. MaxiGala on different planting depth.

2016

Recombination events in full genome sequences of apple stem grooving virus.

2016