A Qualitative Study on Successful African American Marriages on How They Create and Repair Attachment Injuries Using Emotional Skillfulness.

The purpose of this phenomenological study was to describe the meanings that Ossie Davis and Ruby Dee Davis attribute to how they have adapted to their marriage and overcame conflict over the years. A purposive sample of this well-known African American married couple, who self-identified as being in a long-term, successful marriage, was used. The subjects of study were married for 56 years. An analysis using Colaizzi's (1978) method revealed 2 themes, with 13 and 2 subthemes respectively. The themes that emerged from the analysis of the formulated meanings were: (1) secrets to a successful marriage; and (2) sources of conflict in marriage. Secrets to a successful marriage included 13 subthemes: (a) egalitarian roles; (b) commitment; (c) forgiveness; (d) communication; (e) love; (f) honesty; (g) understanding the struggles of Black males and females; (h) friendship; (i) religion/ support from God; (j) compromise; (k) beliefs that marriage is a process; (l) emotional availability of spouse; and (m) feelings of security. In addition, the theme sources of conflict in marriage had two subthemes: (a) different decision making styles; and (b) experiences of abandonment. These findings provided insight from this couple's perspective on the secrets to a successful marriage and the ways in which they managed to make their marriage work, in light of the unique challenges that face African American marriages.

Adult Attachment Style on the MMPI-2: Does an Elevated Scale 4 Predict Dismissing Attachment?

This study examines some aspects of Caldwell's (200 I) hypotheses about the adaptations associated with elevations on the clinical scales of the Minnesota Multiphasic Personality Assessment-2 (MMPI-2). The argument is made that the adaptation to early rejection and neglect, which he attributes to Scale 4, parallels the adaptation represented by a dismissing attachment. Archival data of 72 individuals who completed assessment batteries that included both the MMPI-2 and that Adult Attachment Projective (AAP) were analyzed to determine if there was a relationship between elevations on Scale 4 and classification as dismissing attachment. No significant relationship was found for the primary hypothesis; however, some small effects were noted in related exploratory analyses. Possible reasons for the lack of a significant result are discussed a well as directions for future research.

Heterofunctional Supports in Enzyme Immobilization: From Traditional Immobilization Protocols to Opportunities in Tuning Enzyme Properties

A heterofunctional support for enzyme immobilization may be defined as that which possesses several distinct functionalities on its surface able to interact with a protein. We will focus on those supports in which a final covalent attachment between the enzyme and the support is achieved. Heterofunctionality sometimes has been featured in very old immobilization techniques, even though in many instances it has been overlooked, giving rise to some misunderstandings. In this respect, glutaraldehyde-activated supports are the oldest multifunctional supports. Their matrix has primary amino groups, the hydrophobic glutaraldehyde chain, and can covalently react with the primary amino groups of the enzyme. Thus, immobilization may start (first event of the immobilization) via different causes and may involve different positions of the enzyme surface depending on the activation degree and immobilization conditions. Other “classical” heterofunctional supports are epoxy commercial supports consisting of reactive covalent epoxy groups on a hydrophobic matrix. Immobilization is performed at high ionic strength to permit protein adsorption, so that covalent attachment may take place at a later stage. Starting from these old immobilization techniques, tailor-made heterofunctional supports have been designed to permit a stricter control of the enzyme immobilization process. The requirement is to find conditions where the main covalent reactive moieties may have very low reactivity toward the enzyme. In this Review we will discuss the suitable properties of the groups able to give the covalent attachment (intending a multipoint covalent attachment), and the groups able to produce the first enzyme adsorption on the support. Prospects, limitations, and likely pathways for the evolution (e.g., coupling of site-directed mutagenesis and thiol heterofunctional supports of enzyme immobilization on heterofunctional supports) will be discussed in this Review.

Amination of enzymes to improve biocatalyst performance: coupling genetic modification and physicochemical tools

Improvement of the features of an enzyme is in many instances a pre-requisite for the industrial implementation of these exceedingly interesting biocatalysts. To reach this goal, the researcher may utilize different tools. For example, amination of the enzyme surface produces an alteration of the isoelectric point of the protein along with its chemical reactivity (primary amino groups are the most widely used to obtain the reaction of the enzyme with surfaces, chemical modifiers, etc.) and even its “in vivo” behavior. This review will show some examples of chemical (mainly modifying the carboxylic groups using the carbodiimide route), physical (using polycationic polymers like polyethyleneimine) and genetic amination of the enzyme surface. Special emphasis will be put on cases where the amination is performed to improve subsequent protein modifications. Thus, amination has been used to increase the intensity of the enzyme/support multipoint covalent attachment, to improve the interaction with cation exchanger supports or polymers, or to promote the formation of crosslinkings (both intra-molecular and in the production of crosslinked enzyme aggregates). In other cases, amination has been used to directly modulate the enzyme properties (both in immobilized or free form). Amination of the enzyme surface may also pursue other goals not related to biocatalysis. For example, it has been used to improve the raising of antibodies against different compounds (both increasing the number of haptamers per enzyme and the immunogenicity of the composite) or the ability to penetrate cell membranes. Thus, amination may be a very powerful tool to improve the use of enzymes and proteins in many different areas and a great expansion of its usage may be expected in the near future.

Writ for the attachment of goods or estate: action brought by Edward Hutchinson, Treasurer of Harvard College, 1731 June 5

Writ of attachment authorizing the Suffolk County Sheriff to seize £150 in money or property from John Orme, George Lawrence, and Samuel Pearce, all of Watertown, in response to action brought by Harvard College Treasurer Edward Hutchinson regarding the bond of John White. The case-specific information is handwritten onto a printed form.

Attachment Parenting : Bindungsorientierte Elternschaft

[s.c.]

Manwood's Treatise of the forest laws : shewing not only the laws now in force, but the original of forests, what they are, and how they differ from chases, parks, and warrens; with all such things as are incident to either: together with the proper terms of art, collected out of the common and statute laws of this realm; as also from the assises and iters of Pickering and Lancaster, and several other ancient and learned authors. Treating also of the office of agistors, beadles, foresters, keepers, rangers, verderors and woodwards, and of the Courts of attachment, & c. With all the variety of cases relating to forests, chases, parks, and warrens; and all the laws concerning the game made, adjudged or repealed, since the year 1665. The whole digested under proper titles in an alphabetical order.

"Appendix. Charta de foresta, of Canvtvs, a Dane, and king of this realm; granted at a Parliament holden at Winchester, anno Dom. 1016 [and other statutes relating to forests]": p. 393-435.

The influence of local attachment with respect to home : a poem, in seven books : and Odes, with other poems /

Mode of access: Internet.

Direct support and general support maintenance manual for tractor, wheeled, DED, loader backhoe, with hydraulic impact tool and with hydraulic earth auger attachment, John Deere model JD410 (CCE), with bucket, impactor, and earth drill (NSN 2420-00-567-0135).

"August 1992."

Organizational maintenance manual (including repair parts and special tools lists) : milling-grinding-drilling-slotting attachment, 115/230-volt, 60-cycle, single-phase, 1/3 horsepower (Dumore Company model 827401) ...

"January 1965."

Operator's manual : grinding machine, machine tool attachment, internal and external grinding, 5" maximum external diameter wheel, 1/4" minimum internal diameter wheel, 4,600 to 42,400 rpm spindle speed, 1/2 hp, AC/DC, 115 V, 60-C, single phase (Dumore series 5) ...

"October 1967."

Operator's manual : grinding machine, machine tool attachment (U.S. Electrical Tool Co. model HLGE-12) (3460-243-9607).

"November 1965."

Operator's manual : tractor, wheeled (DED) loader backhoe w/hydraulic impact tool and w/hydraulic earth auger attachment, John Deere model JD410 (CCE), w/Wain-Roy Bucket, Hughes Impactor and Danuser Earth Drill, (NSN 2420-00-567-0135).

Includes index.