Comparative study for hardwood and softwood forest biomass: Chemical characterization, combustion phases and gas and particulate matter emissions

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Clinical feasibility of cognitive testing in dogs (Canis lupus familiaris)

Several cognitive tests have been developed to evaluate specific aspects of human and animal learning and memory. These tests have been used for early detection of cognitive deficits and to monitor the treatment of dogs with cognitive impairment. Thus, this article evaluated the feasibility of cognitive tests for use in canine neurology clinical routines and the suitability of the different tests to accomplish this aim. Fifteen healthy adult dogs were used for the cognitive tests of reward approach learning, object approach learning, object discrimination learning, reversal learning, delayed non-matched to position, and delayed non-matched to sample to assess different aspects of memory. No difference was observed between tests of delayed non-matched to position (3.13 +/- 2.23 days) and delayed non-matched to sample (3.20 +/- 2.40 days) (P = 0.944). However, dogs had greater difficulty in reversal learning (8.47 +/- 2.61 days) than in object discrimination learning (4.60 +/- 1.64 days) (P <= 0.001). Based on the tests performed, the delayed non-matched to position test may be performed in clinical routine if the owner and the veterinarian have time available, because this test is sensitive to evaluate dogs with cognitive impairments, but requires approximately 10 days of training. Thus, elderly dogs are excellent experimental models to study pathological aging based on their similarities with some human brain diseases, such as Alzheimer disease. (C) 2014 Elsevier Inc. All rights reserved.

Two-stage rumenostomy in buffaloes

Background: Rumenostomy may be performed for therapeutic and digestibility research purposes in bovines, small ruminants and camelids. Several studies requires romenostomy in buffaloes in order to sample ruminal content for laboratorial assays. However, complications and outcome of rumenostomy was poorly studied in buffaloes. Therefore, the aim of the current study was to describe a two-stage rumenostomy technique in buffaloes, focused on intra and post-operative period.Materials, Methods & Results: Nine Murrah buffaloes were submitted to a 36-h and 12-h of food and water fastening. The animals were given acepromazine and maintained in standing position. Flank local anesthesia was carried out. A circular skin incision was carried out in the center of the left flank, followed by divulsion of the external and internal obliques and transversus abdominus muscles, and incision of the peritoneum. Subsequently, a segment of the dorsal aspect of the rumen was grasped and pulled through the flank incision. The rumen was attached to the peritoneum and skin incision margins in four points (dorsal, ventral, cranial and caudal). Additional simple interrupted sutures attaching the rumen serosa to the skin were applied subsequently. Four additional interrupted horizontal mattress sutures were applied equidistantly, taking bites only in the skin and rumen serosa. Following 12 h, the second stage was carried out. The buffaloes were prepared and restrained as performed for the first stage. A circular flap was excised from the exteriorized rumen and the silicone romenostomy cannula was placed. Clinical parameters, postoperative recovery, weight and behavioral pain scale were assessed. Positioning and anesthesia regimen were adequate for the achievement of the procedure. However, two animals fell in the restraint chute during the first surgical stage. Mild ischemia of the exteriorized rumen segment was observed on the second surgical stage, which resulted in less hemorrhage and enhanced cannula positioning. Complete cicatrization and permanent adhesion of the rumen to the skin were achieved. No ruminal leakage to the abdominal cavity occurred. No signs of pain were reported. There were few cases of laxity of the romenostomy opening leading to drop of cannula, myiasis on the margin of the stoma site and few cases of mild ruminal content leakage on the long-term assessment.Discussion: Restraint in standing position was considered adequate, although lateral recumbence constitutes another option. However, higher risk of contamination and technical difficulties in placing the cannulas are expected if lateral recumbence is considered. In other trials using acepromazine, no accidental recumbence occurred. Xylazine was also indicated for chemical restraint of buffaloes. It is known that flexible cannulas provide better anatomic adjustment and adaptation as well as being effective for sampling ruminal content, as seen in the current study. Ruminal leakage is one of the most frequent complications of romenostomy, which may affect animal's welfare. The animals in the current study presented no variations on the body score, even though on those presenting cannula loosening or ruminal content leakage. Moreover, no significant changes of the ruminal content parameters were noticed. Myiasis was also reported following ruminal surgical interventions, which were mainly attributed to extensive breeding. Loss of the cannula, subcutaneous emphysema and suture dehiscence are common complications of romenostomy. Nonetheless, none of those complications were found on the current study. Thus, romenostomy was feasible and efficient for sampling and performing assays of the ruminal content in buffaloes.

Comparando a eficácia da manta térmica com método de aquecimento passivo na prevenção de hipotermia

Hypothermia is a common phenomenon in the perioperative period, and it affects 60 to 90% of patients submitted to anesthetic-surgical procedures. In order to minimize its incidence, warming methods are used. Such methods can be passive, such as orthopedic cotton, sheets and blankets, or active, such as warm-air blankets and thermal mattresses. In this scenario, the present study aimed at comparing two warming methods used in the intraoperative period. Patients submitted to abdominal surgery in the specialties of gynecology and gastric surgery from August to September 2010 were included in the study. After randomization, they were divided into two groups: one using a thermal blanket (group I) and one using orthopedic cotton (group II). At last, 9 patients were included. The variables for each question were considered according to occurrence frequency. Comparison between groups was performed by Student’s t test. With the purpose to analyze whether there was an association, the chi-square test or Fisher’s Exact test was used. Whenever it was applicable for multiple comparisons, Tukey’s test was utilized; p values < 0.05 were considered to be statistically significant for analysis. The sample comprised 6 males and 3 females submitted to gynecological and gastric surgeries. Their mean age was 48 years for group I and 46.2 years for group II. A predominance of general anesthesia was observed. The time of permanence in the operating room ranged from 80 to 360 minutes. With regard to warming parenteral solutions, the procedure was performed on 5 patients, and infusion of warm solution into the abdominal cavity was performed on 50% of the sample. Concerning the warming method used, 5 patients used a thermal blanket. In view of the results presented, it was not possible to conclude which warming method should be used due to sample size

Espectrometria de massa para análise isotópica dos bio-elementos

Mass spectrometry is an analytical technique widely used in several areas of academic research. It allows the knowledge of the information about the micro-world of atoms leading to significant advances in science today. The analysis of stable isotopes of carbon, hydrogen, oxygen, nitrogen and sulfur, also known as bio-elements, shows itself as a major area of interest in using the proposed method. The development of techniques and equipment coupled with mass spectrometry promises to deliver even greater progress in this field, in particular, for the biological sciences and related areas. The pyrolytic method in reduction of organic compounds at high temperatures provides simultaneous isotopic analysis of bio-elements H and O, by the gases released, H2 and CO after the pyrolitic process, significantly reducing analysis time and the amount of material to sample. This paper presents a review of mass spectrometry with its basic principles of operation, and pyrolytic method for reducing compounds at temperatures above 1400 ° C for isotopic analysis of bio-elements

Predictors of Burnout Syndrome in Dentistry Students

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito Agudo em Parâmetros Cinéticos em Tarefas Estáticas e Dinâmicas devido ao Uso de Palmilhas Proprioceptivas

Pós-graduação em Engenharia Mecânica - FEG

Real-time Sampling of Particulate Matter Smaller than 2.5 µm from Amazon Forest Biomass Combustion

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Jato de plasma frio em pressão atmosférica para tratamento de materiais e esterilização

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Low-temperature plasma: an effective approach against Candida albicans biofilm

This study evaluated the antifungal potential of low-temperature plasma (LTP) on a 72-hour Candida albicans biofilm. A growth inhibition zone test was conducted with agar plates inoculated with C. albicans and submitted to LTP and argon application at 3 and 10 mm for 10, 30, 60, 90, and 120 seconds. The groups for biofilm assays were 60 seconds of LTP application with a tip-to-sample distance of 3 mm (LTP-3) and 10 mm (LTP-10); –application of only argon gas for 60 seconds with a tip-to-sample distance of 3 mm (Ar-3) and 10 mm (Ar-10); and no treatment. The C. albicans biofilm was grown on saliva-coated discs. The medium was replaced every 24 hours. Confocal laser scanning microscopy revealed the proportion of live and dead cells, and variable pressure scanning electron microscopy (VPSEM) showed biofilm/cell structure. No inhibition zone was observed for control and either Ar groups. For the LTP groups, a progressively increasing of inhibition zone diameter was observed for different treatment durations. The LTP-3 and LTP-10 groups presented higher proportions of dead cells compared with the Ar-3 and Ar-10 groups. VPSEM revealed cell perforations in the LTP-3 and LTP-10 groups. A short period of LTP exposure demonstrated an antifungal effect on C. albicans biofilm.

Análise da cinética do VO2 no domínio severo do crawl em condição atada de nado

Pós-graduação em Desenvolvimento Humano e Tecnologias - IBRC

Efeitos de instruções preliminares sobre o seguimento de regras

O presente estudo teve como objetivo testar os efeitos de diferentes instruções preliminares sobre o seguimento de regras. Dezoito estudantes universitários foram expostos a um procedimento modificado de escolha de acordo com o modelo e distribuídos em três grupos, que diferiram quanto às instruções preliminares recebidas (completa, sem trechos sobre materiais ou sem trechos sobre conseqüências). Os participantes de cada grupo foram divididos em duas condições experimentais, em que instruções correspondentes ou discrepantes foram apresentadas. Os resultados mostraram que a ausência de trechos sobre materiais dificulta a realização da tarefa e a ausência de trechos sobre as conseqüências diminui a variação de respostas, porém todos os participantes que realizaram corretamente a tarefa seguiram tanto instruções correspondentes quanto discrepantes.

Food Content of Six Commercial Fishes from Mississippi Sound

Specific dietary contents from six fishes collected in Mississippi Sound are recorded. In order of their importance, primary components grouped in major taxonomic categories were fishes, penaeid shrimps, and other crustaceans for Cynoscion nebulosus; Crustaceans and fishes for C. arenarius; fishes and crustaceans for C. nothus; crustaceans, pelecypods, and polychaetes for Pogonias cromis; crustaceans, molluscs, polychaetes, and fishes for Archosargus probatocephalus; and fishes and penaeid shrimps for Paralichthys lethostigma. Principal items in the diets of most of the fishes included Anchoa mitchilli, Penaeus aztecus, P. setiferus, and Callinectes sapidus. Those crustaceans show that competition exists for commercial shellfishes in Mississippi Sound. Ratios among the different dietary items vary, according at least to species of fish, length of fish, season, specific location, and abundance of available prey. Some of these variations are documented and are additionally related to selected findings by other authors sampling different localities. We suggest that examination of food items in Archosargus probatocephalus can serve as a practical means to sample and assess seasonal prevalence and abundance of a wide range of invertebrates throughout different habitats in Mississippi Sound and elsewhere.

Radiocarbon Dating, Chronologic Framework, and Changes in Accumulation Rates of Holocene Estuarine Sediments from Chesapeake Bay

Rapidly accumulating Holocene sediments in estuaries commonly are difficult to sample and date. In Chesapeake Bay, we obtained sediment cores as much as 20min length and used numerous radiocarbon ages measured by accelerator mass spectrometry methods to provide the first detailed chronologies of Holocene sediment accumulation in the bay. Carbon in these sediments is a complex mixture of materials from a variety of sources. Analyses of different components of the sediments show that total organic carbon ages are largely unreliable, because much of the carbon (including coal) has been transported to the bay from upstream sources and is older than sediments in which it was deposited. Mollusk shells (clams, oysters) and foraminifera appear to give reliable results, although reworking and burrowing are potential problems. Analyses of museum specimens collected alive before atmospheric nuclear testing suggest that the standard reservoir correction for marine samples is appropriate for middle to lower Chesapeake Bay. The biogenic carbonate radiocarbon ages are compatible with 210Pb and 137Cs data and pollen stratigraphy from the same sites. Post-settlement changes in sediment transport and accumulation is an important environmental issue in many estuaries, including the Chesapeake. Our data show that large variations in sediment mass accumulation rates occur among sites. At shallow water sites, local factors seem to control changes in accumulation rates with time. Our two relatively deep-water sites in the axial channel of the bay have different long-term average accumulation rates, but the history of sediment accumulation at these sites appears to reflect overall conditions in the bay. Mass accumulation rates at the two deep-water sites rapidly increased by about fourfold coincident with widespread land clearance for agriculture in the Chesapeake watershed.

Gelatinous zooplankton fauna (Cnidaria, Ctenophora and Thaliacea) from Baia da Babitonga (southern Brazil)

The present study reports on a survey of the gelatinous zooplankton fauna (Cnidaria, Ctenophora and Thaliacea) from the proposed Baia da Babitonga marine protected area (southern Brazil; similar to 26 degrees S), based on collections from multiple sites over different seasons and from published literature. In order to sample both small and large gelatinous animals, plankton hauls (n = 255) and fishing trawls (n = 126) were employed. More than 20,000 organisms were studied, which, including literature data, totaled 48 species: one cubomedusa, three scyphomedusae, four siphonophores, 36 hydromedusae, two ctenophores, and two thaliaceans. Among these, the hydromedusae Cnidostoma fallax Vanhoffen and Helgicirrha sp. are recorded for the first time from the southwestern Atlantic coast and Paulinum sp. and Protiara sp. are recorded for the first time from the South Atlantic. A description of young stages of the hydromedusa Gossea brachymera Bigelow is presented and shows that Octobulbacea montehermosensis Zamponi is a junior synonym of the former. Although comprehensive local assessment of diverse taxonomic groups is still lacking, the high diversity observed herein underscores the importance of Ba a da Babitonga as a high priority site for conservation of regional marine biodiversity.