Tafonomia de biválvios em calcários oolíticos da formação Teresina (Bacia do Paraná, Permiano Médio, Prudentópolis, PR)

Herein, it is presented the first detailed taphonomic study on bivalve mollusk shells preserved in the oolitic limestones of the Teresina Formation (probably Kungurian-Roadian, Lower-Middle Permian) in the eastern margin of the Parana basin. The selected beds are located in two quarries (informally named PRU 1 and PRU 2) in Prudentopolis municipality (Center-South Parana State), and positioned approximately in the middle of the formation and probably in the Pinzonella illusa Zone. The PRU 1 limestone ([approximately]30 cm thick), which is partially silicified and intercalated with predominantly pelitic rocks, is classified as a bivalve oolitic grainstone. The basal contact is erosive and the top shows symmetrical ripple marks, which are draped by shale with mud cracks. There are two fining-upwards successions characterized by dense to dispersed packing of the shells, which are usually disarticulated, randomly oriented (many nested/stacked) and mixed with some Formapelitic intraclasts. Microhummocky cross-stratification occurs a little below the top of the bed. The PRU2 bed is classified as ooidbivalve rudstone[approximately] (~5 cm thick), where all shells are disarticulated and fragmented, showing dense packing. The bivalves probably inhabited a muddy substrate and were mixed (as parautochtonous and allochthonous bioclasts) with ooids during high-energy storm events, including posterior shell displacement as a result of bioturbation. Thus, the calcareous beds represent amalgamated proximal tempestites with a complex taphonomic history, strong temporal/spatial mixing of bioclasts and limited paleoecological resolution. They are a typical example of shell beds generated in a huge epeiric sea, which was not necessarily connected to the ocean and where very low depositional-slope gradient, very slow subsidence and minimum sediment accommodation space caused frequent sediment reworking by storm related processes.

The invasion of five alien species in the Delta do Parnaiba Environmental Protection Area, Northeastern Brazil

Marine biological invasions have been regarded as one of the major causes of native biodiversity loss, with shipping and aquaculture being the leading contributors for the introductions of alien species in aquatic ecosystems. In the present study, five aquatic alien species (one mollusk, three crustaceans and one fish species) were detected during dives, shore searches and from the fisheries on the coast of the Delta do Parnaiba Environmental Protection Area, in the States of Piaui and Maranhao, Northeastern Brazil. The species were the bicolor purse-oyster Isognomon bicolor, the whiteleg shrimp Litopenaeus vannamei, the giant river prawn Macrobrachium rosenbergii, the Indo-Pacific swimming crab Charybdis hellerii and, the muzzled blenny Omobranchus punctatus. Ballast water (I. bicolor, C. hellerii, and O. punctatus) and aquaculture activities (L. vannamei and M. rosenbergii) in adjacent areas are the most likely vectors of introduction. All exotic species found have potential impact risks to the environment because they are able to compete against native species for resources (food and habitat). Isognomon bicolor share the same habitat and food items with the native bivalve species of mussels and barnacles. Litopenaeus vannamei share the same habitat and food items with the native penaeids such as the pinkspot shrimp Farfantepenaeus brasiliensis, the Southern brown shrimp Farfantepenaeus subtilis, and the Southern white shrimp Litopenaeus schmitti, and in the past few years L. vannamei was responsible for a viral epidemics in the cultivation tanks that could be transmitted to native penaeid shrimps. Charybdis hellerii is also able to cause impacts on the local fisheries as the species can decrease the populations of native portunid crabs which are commercialized in the studied region. Macrobrachium rosenbergii may be sharing natural resources with the Amazon River prawn Macrobrachium amazonicum. Omobranchus punctatus shares habit with the native redlip blenny Ophioblennius atlanticus and other fishes, such as the frillfin goby Bathigobius soporator. Some immediate remedial measures to prevent further introductions from ballast water and shrimp farm ponds should be: (i) to prevent the release of ballast water by ship/vessels in the region; (ii) to reroute all effluent waters from shrimp rearing facilities through an underground or above-ground dry well; (iii) to install adequate sand and gravel filter which will allow passage of water but not livestock; (iv) outdoor shrimp pounds located on floodable land should be diked, and; (v) to promote environmental awareness of those directly involved with ballast water (crews of ship/vessels) and shrimp farms in the region. Rev. Biol. Trop. 58 (3): 909-923. Epub 2010 September 01.

SPASMOGENIC NONCHOLINERGIC ACTIVITY OF A DIGESTIVE GLAND HOMOGENATE OF THE AMPHIBIAN SNAIL POMACEA-LINEATA SPIX-1827

The digestive gland of Pomacea lineata, a prosobranch gastropod mollusc inhabiting both fresh water and land, does not contain cholinomimetic compounds as do the glands of species of Aplysia, marine opisthobranch gastropods, in which both acetylcholine and urocanylcholine are present. The only pharmacological action detected for the digestive gland of Pomacea was spasmogenic activity of a crude homogenate containing 0.1 g tissue equivalents on the snail's own esophagus bathed in 10 ml of a physiological solution prepared on the basis of the animal's hemolymph composition. The spamodic activity was not blocked by atropine, bromlysergic acid diethylamide or anthazoline.

Quantitative estimates of time-averaging in terebratulid brachiopod shell accumulations from a modern tropical shelf

Quantitative estimates of time-averaging in marine shell accumulations available to date are limited primarily to aragonitic mollusk shells. We assessed time-averaging in Holocene assemblages of calcitic brachiopod shells by direct dating of individual specimens of the terebratulid brachiopod Bouchardia rosea. The data were collected from exceptional (brachiopod-rich) shell assemblages, occurring surficially on a tropical mixed carbonate-siliciclastic shelf (the Southeast Brazilian Bight, SW Atlantic), a setting that provides a good climatic and environmental analog for many Paleozoic brachiopod shell beds of North America and Europe. A total of 82 individual brachiopod shells, collected from four shallow (5-25 m) nearshore (<2.5 km from the shore) localities, were dated by using amino acid racemization (D-alloisoleucine/L-isoleucine value) calibrated with five AMS-radiocarbon dates (r(2) = 0.933). This is the first study to demonstrate that amino acid racemization methods can provide accurate and precise ages for individual shells of calcitic brachiopods.The dated shells vary in age from modern to 3000 years, with a standard deviation of 690 years. The age distribution is strongly right-skewed: the young shells dominate the dated specimens and older shells are increasingly less common. However, the four localities display significant differences in the range of time-averaging and the form of the age distribution. The dated shells vary notably in the quality of preservation, but there is no significant correlation between taphonomic condition and age, either for individual shells or at assemblage level.These results demonstrate that fossil brachiopods may show considerable time-averaging, but the scale and nature of that mixing may vary greatly among sites. Moreover, taphonomic condition is not a reliable indicator of pre-burial history of individual brachiopod shells or the scale of temporal mixing within the entire assemblage. The results obtained for brachiopods are strikingly similar to results previously documented for mollusks and suggest that differences in mineralogy and shell microstructure are unlikely to be the primary factors controlling the nature and scale of time-averaging. Environmental factors and local fluctuations in populations of shell-producing organisms are more likely to be the principal determinants of time-averaging in marine benthic shelly assemblages. The long-term survival of brachiopod shells is incongruent with the rapid shell destruction observed in taphonomic experiments. The results support the taphonomic model that shells remain protected below (but perhaps near) the surface through their early taphonomic history. They may be brought back up to the surface intermittently by bioturbation and physical reworking, but only for short periods of time. This model explains the striking similarities in time-averaging among different types of organisms and the lack of correlation between time-since-death and shell taphonomy.

Fidelity of molluscan assemblages from the touro passo formation (pleistocene-holocene), southern brazil: taphonomy as a tool for discovering natural baselines for freshwater communities

This study is the first assessment of mollusk fossil assemblages relative to the compositional fidelity of modern mollusk living and death assemblages. It also shows that the sedimentary record can provide information on the original, non-human-impacted, freshwater malacofauna biodiversity, based on Late Pleistocene shells. The fossil mollusk assemblage from the Touro Passo Formation (Pleistocene-Holocene) was compared to living and death assemblages of the Touro Passo River, southern Brazil, revealing little resemblance between fossil and live-dead species composition. Although the living and death assemblages agree closely in richness, species composition, and species relative abundances (both proportional and rank), the fossil assemblage differs significantly from both modern assemblages in most of these measures. The fossil assemblage is dominated by the native endemic corbiculid bivalve Cyanocyclas limosa and the gastropod Heleobia aff. bertoniana. These are absent in the living assemblages, and both living and death assemblages are dominated by the alien Asiatic corbiculid C. fluminea, which is absent in the fossil assemblage. The fossil assemblage also contains, overall, a higher proportional abundance of relatively thick-shelled species, suggesting a genuine bias against the thinner- and smaller-shelled species. Our results suggest that contemporary environmental changes, such as the introduction of some alien freshwater mollusk species, together with post-burial taphonomic processes, are the main factors leading to the poor fidelity of the fossil assemblage studied. Hence, the taxonomic composition of the Late Pleistocene mollusks from the Touro Passo Formation probably would show greater similarity to present-day assemblages wherever the mollusk biodiversity is not disturbed by human activities.

Feeding activity of Callinectes ornatus Ordway, 1863 and Callinectes danae Smith, 1869 (Crustacea, Brachyura, Portunidae) in Ubatuba, SP, Brazil

The feeding activity along the day cycle and the time consumed for extracellular digestion were evaluated in the portunids C. ornatus and C. danae. Swimming crabs were obtained from trawling in Ubatuba bay, São Paulo, Brazil, during both the rainy and dry seasons. In each season, daily scheduled samples were taken at dawn (±6 h), noon (±12 h), dusk (±18 h) and midnight (±24 h). All individuals were dissected and the degree of stomach replenishment was recorded. In order to estimate the time elapsed for extracellular digestion, crabs were fed, and groups were dissected at 30 min intervals to check the conditions of their stomachs. In general, both species show a higher feeding activity during periods of lower light intensity, as evidenced by an increased percentage of full stomachs in dusk and midnight samples. The obtained results support higher feeding activity at night in these species and indicate short time for extracellular digestion, not exceeding 8 h. Nevertheless, full stomachs were recorded in all sampling schedules. In this case, it should be considered that elimination of certain food items such as fish bones, mollusk shells and carapace fragments of crustaceans could take more time than other items. Additionally, some crab species could require a cycle of cell replacement in the midgut gland epithelium until they can take their next meal.

Toxicidade aguda do sulfato de cobre e do extrato aquoso de folhas secas de nim para o caramujo (Pomacea canaliculata)

Snails can become an environmental and economic problem, causing substantial losses. The objective of this work was to estimate the acute toxicity of copper sulfate pentahydrate (CuSO4.5H2O) and the aqueous extract of dried neem leaves on snails (P. canaliculata) under laboratory conditions. In order to estimate the lethal concentration 50% (LC (I)50;96h), snails were exposed to six increasing copper sulfate concentrations (0.0; 0.01; 0.03; 0.05; 0.07 and 0.1 mg L-1) and six increasing concentrations of aqueous extract of dried neem leaves 0.0; 100; 125; 150; 175 and 200 mL aqueous extract of dried neem leaves L-1 water, equivalent to (0.0; 1.18; 1.47; 1.77; 2.06 and 2.36 mg azadirachtin L -1), in triplicate and one control treatment in an entirely random delineation. Estimated LC (I)50;96h, of copper sulfate was 0.02 mg copper sulfate L-1, with a 0.01 mg L-1 lower limit and a 0.03 mg L-1 upper limit. Estimated lethal concentration 50% of the aqueous extract of dried neem leaves was 142.75 mL L-1, equivalent to 1.68 mg L-1 of azadirachtine, with a 130.89 mL L-1 (1.54 mg L-1) low limit and 155.69 mL L-1 (1.83 mg L -1) as the upper limit.

A new permian bivalve (Megadesmidae, Plesiocyprinellinae) from the Serrinha member, Rio do Rasto Formation, Paraná Basin, Brazil

During a recent inspection in the Paleontological Collection of the Institute of Geosciences, University of São Paulo, we have identifi ed some specimens of undescribed mollusk bivalves. These called our attention for the following reasons: a) all specimens are internal molds of conjugated and closed articulated valves, some of them presenting fragments of silicifi ed shells; b) all internal molds have similar general shape and internal characters, representing specimens of the same taxon; c) the internal molds and silicifi ed valves are well preserved, including fragile structures, which are hardly preserved, such as the internal mold of the external ligament and muscle scars; d) and equally important, according to the labels of all specimens, they were collected from rocks of the Passa Dois Group (Permian), Serrinha Member of the Rio do Rasto Formation. Although who collected the shells and the precise geographic location of the specimens are still unknown, the detailed study of these fossils brings us to the conclusion that they are morphologically distinct from any heretofore published genus of the endemic fauna of bivalves from Passa Dois Group. Based in its general shape, hinge structure and muscles scars, the new form can be classifi ed under the Family Megadesmidae Vokes, 1967, the most diverse group of Permian bivalves of the Paraná Basin. The specimens are referred as Beurlenella elongatella new gen. and sp. The shell shape and taphonomy indicate that this bivalve was a shallow, rapid, active burrower, suspension feeder, probably preserved in situ, in event deposits.

Determinação da entalpia e da entropia de solvatação da superfície protéica a partir da energética de oxigenação de hemoglobinas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação e comparação de projetos comunitários de ostreicultura localizados no nordeste paraense

A aqüicultura vem sendo caracterizada como estratégia para minimizar a redução do pescado. no Pará, a ostreicultura foi implantada em 2003 como oportunidade de ocupação e renda no litoral nordeste paraense, em 2005 esta iniciativa foi estendida para outras comunidades com diferentes níveis de familiaridade com o molusco, seja pelo apoio na realização de pesquisas no setor ou pela atividade extrativista. A pesquisa avaliou o desempenho da ostreicultura comunitária no estado do Pará, foram estudados três projetos com pelo menos dois anos em atividade e com diferentes níveis de preservação e atividade extrativista de ostra. A implantação dos primeiros cultivos ocorreu simultaneamente e com similaridade de apoio institucional, mas com diferentes desempenhos, as principais dificuldades dos produtores é de obter licença para atividade e a necessidade de cuidados com a variação de maré. É necessário que estudos de viabilidade sejam mais rígidos, com maior comprometimento das instituições interessadas no setor em realizar as ações que as compete, realizar eventos onde possam ser disseminadas informações e discutidos os gargalos da aqüicultura no Pará e a formação de uma sólida rede de parceiros para capacitar os grupos na organização, gestão das atividades e auxilio no acesso ao mercado. Os criadores de ostras, na sua maioria, são pequenos agricultores, pescadores artesanais, que têm na ostreicultura uma forma de suplementar a renda familiar, ao mesmo tempo como uma maneira de conseguir alimento para seus familiares. No entanto, a ostreicultura é uma atividade secundária para os mesmos, pois ainda não oferece condições de mantê-los. Mas vários criadores já declararam que a partir do momento que conseguirem aumentar a produção de ostras se dedicarão exclusivamente a essa atividade.

Caracterização de Aeromonas spp isoladas de amostras de ostras e água por método microbiológico e molecular

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Ocorrência de Angiostrongylus cantonensis (Nematoda, Angiostrongylidae) em Achatina fulica (Mollusca, Gastropoda) na Baixada Santista

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Quantificação de metais potencialmente tóxicos em mexilhão dourado (Limnoperna fortunei) por espectrometria de absorção atômica com fonte contínua e alta resolução empregando amostragem direta de sólidos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Radiocarbon Dating, Chronologic Framework, and Changes in Accumulation Rates of Holocene Estuarine Sediments from Chesapeake Bay

Rapidly accumulating Holocene sediments in estuaries commonly are difficult to sample and date. In Chesapeake Bay, we obtained sediment cores as much as 20min length and used numerous radiocarbon ages measured by accelerator mass spectrometry methods to provide the first detailed chronologies of Holocene sediment accumulation in the bay. Carbon in these sediments is a complex mixture of materials from a variety of sources. Analyses of different components of the sediments show that total organic carbon ages are largely unreliable, because much of the carbon (including coal) has been transported to the bay from upstream sources and is older than sediments in which it was deposited. Mollusk shells (clams, oysters) and foraminifera appear to give reliable results, although reworking and burrowing are potential problems. Analyses of museum specimens collected alive before atmospheric nuclear testing suggest that the standard reservoir correction for marine samples is appropriate for middle to lower Chesapeake Bay. The biogenic carbonate radiocarbon ages are compatible with 210Pb and 137Cs data and pollen stratigraphy from the same sites. Post-settlement changes in sediment transport and accumulation is an important environmental issue in many estuaries, including the Chesapeake. Our data show that large variations in sediment mass accumulation rates occur among sites. At shallow water sites, local factors seem to control changes in accumulation rates with time. Our two relatively deep-water sites in the axial channel of the bay have different long-term average accumulation rates, but the history of sediment accumulation at these sites appears to reflect overall conditions in the bay. Mass accumulation rates at the two deep-water sites rapidly increased by about fourfold coincident with widespread land clearance for agriculture in the Chesapeake watershed.

Aves marinas (Procellariidae) del Pleistoceno del yacimiento paleontológico de la Montaña de Timbaiba (Lanzarote, Islas Canarias)

[ES] Se describe la concurrencia de restos fósiles pertenecientes a dos taxones avianos de pardelas (Procellariidae) provenientes del yacimiento paleontológico de Montaña de Timbaiba, del Pleistoceno Medio, en la isla de Lanzarote. El conjunto está copado mayoritariamente por la especie extinta Puffinus holeae y se constata la presencia de la especie actual Calonectris diomedea. Se interpreta el yacimiento como un posible lugar de nidificación y cría de estas especies de pardelas.