Isolation of virus-neutralizing RNAs from a large pool of random sequences.

RNA and ribonuclease-resistant RNA analogs that bound and neutralized Rous sarcoma virus (RSV) were isolated from a large pool of random sequences by multiple cycles of in vitro selection using infectious viral particles. The selected RNA pool of RSV-binding sequences at a concentration of 0.16 microM completely neutralized the virus. Of 19 sequences cloned from the selected pool, 5 inhibited RSV infection. The selected RNA and RNA analogs were shown to neutralize RSV by interacting with the virus, rather than by adversely affecting the host cells. The selection of the anti-RSV RNA and RNA analogs by intact virions immediately suggests the potential application of this approach to develop RNA and RNA analogs as inhibitors of other viruses such as human immunodeficiency virus.

Optimized methods for extracting circulating small RNAs from long-term stored equine samples.

Circulating miRNAs in body fluids, particularly serum, are promising candidates for future routine biomarker profiling in various pathologic conditions in human and veterinary medicine. However, reliable standardized methods for miRNA extraction from equine serum and fresh or archived whole blood are sorely lacking. We systematically compared various miRNA extraction methods from serum and whole blood after short and long-term storage without addition of RNA stabilizing additives prior to freezing. Time of storage at room temperature prior to freezing did not affect miRNA quality in serum. Furthermore, we showed that miRNA of NGS-sufficient quality can be recovered from blood samples after >10 years of storage at -80 °C. This allows retrospective analyses of miRNAs from archived samples.

The sidereal messenger.

Vol. 1, no. 7 and no. 8 are paginated so that v. 1, no. 8 comes before v. 1, no. 7.

The Messenger of mathematics

Mode of access: Internet.

The Messenger of peace.

Publication suspended, 1895-1899.

The Messenger.

Mode of access: Internet.

The Boston weekly messenger, a journal of politics, agriculture, literature and miscellaneous intelligence.

Mode of access: Internet.

Poe and the Southern literary messenger,

Mode of access: Internet.

The Western literary messenger.

Published: Buffalo [N.Y.] : Thomas & Lathrop, Sept. 1854-

The Western messenger.

Editors: Nov. 1837-Oct. 1839, J.F. Clarke (May-Oct. 1839 assisted by W.H. Channing and J.H. Perkins.

The Dairy messenger.

Mode of access: Internet.

Illinois health messenger.

None published: 1954-1955.

The mégha dúta, or, cloud messenger : a poem, in the Sanscrit language /

Translation of: Meghadūta.

Dissertations on the regenerate life ; in harmony with the theological views of Baron Swedenborg, the undoubted messenger of our Lord's second advent. To which is prefixed, A short account of the life of Baron Swedenborg.

Includes bibliographical references.

Dissertations on the regenerate life ; in harmony with the theological views of Emanuel Swedenborg, the undoubted messenger of our Lord's second advent /

Mode of access: Internet.