Thy-1-mediated cell-cell contact induces astrocyte migration through the engagement of αVβ3 integrin and syndecan-4.

Cell adhesion to the extracellular matrix proteins occurs through interactions with integrins that bind to Arg-Gly-Asp (RGD) tripeptides, and syndecan-4, which recognizes the heparin-binding domain of other proteins. Both receptors trigger signaling pathways, including those that activate RhoGTPases such as RhoA and Rac1. This sequence of events modulates cell adhesion to the ECM and cell migration. Using a neuron-astrocyte model, we have reported that the neuronal protein Thy-1 engages αVβ3 integrin and syndecan-4 to induce RhoA activation and strong astrocyte adhesion to their underlying substrate. Thus, because cell-cell interactions and strong cell attachment to the matrix are considered antagonistic to cell migration, we hypothesized that Thy-1 stimulation of astrocytes should preclude cell migration. Here, we studied the effect of Thy-1 expressing neurons on astrocyte polarization and migration using a wound-healing assay and immunofluorescence analysis. Signaling molecules involved were studied by affinity precipitation, western blotting and the usage of specific antibodies. Intriguingly, Thy-1 interaction with its two receptors was found to increase astrocyte polarization and migration. The latter events required interactions of these receptors with both the RGD-like sequence and the heparin-binding domain of Thy-1. Additionally, prolonged Thy-1-receptor interactions inhibited RhoA activation while activating FAK, PI3K and Rac1. Therefore, sustained engagement of integrin and syndecan-4 with the neuronal surface protein Thy-1 induces astrocyte migration. Interestingly we identify here, a cell-cell interaction that despite initially inducing strong cell attachment, favors cell migration upon persistent stimulation by engaging the same signaling receptors and molecules as those utilized by the extracellular matrix proteins to stimulate cell movement.

Pseudophakic Retinal Detachment Surgery by 23 G Vitrectomy using Slit-Lamp and Non-Contact 90 D Lens.

Background: The purpose of this study is to report the anatomic and functional results of primary 23 G vitrectomy using slit-lamp and non-contact 90 D lens for the treatment of pseudophakic rhegmatogenous retinal detachment. Patients and Methods: Pseudophakic eyes were operated by 23 G vitrectomy using slit-lamp and non-contact 90 D lens, internal subretinal fluid drainage, cryopexy and internal gas tamponade. The preoperative and postoperative characteristics were analysed. Main outcome measures were anatomic success rates after initial surgical intervention and after reoperation for primary failures, visual outcome at the last follow-up visit, and complications. Results: 46 pseudophakic eyes were included in this retrospective study (October 2013- January 2014). In 40 cases, sulfur hexafluoride 23 % gastamponade was used, silicone oil in 6 cases (13 %). The retina was reattached successfully after a single surgery in 44 eyes (96 %). Recurrence of retinal detachment occurred in 2 eyes. Final anatomic reattachment was obtained in 100 % after a second operation. Silicone oil was removed in all eyes. Visual acuity improved significantly from logMAR 0 (IQR 0 - 0.9) to logMAR 0 (IQR 0 - 0.2) (p < 0.005). Conclusions: Primary 23 G vitrectomy using slit-lamp and non contact 90 D lens for the treatment of pseudophakic rhegmatogenous retinal detachment provides a high anatomic and functional success rate and is associated with few complications.

[Archives de la Parole]. , [Parable of the prodigal son] : récit en hindoustani [mēwātī] / [Sir George Grierson], éd. ; Linguistic survey of India ; [Jainti Pershad], voix

[Traditions. Asie. Inde. Province de Delhi]

[Archives de la Parole]. , Récit en hindoustani [urdū] / [Sir George Grierson], éd. ; Linguistic survey of India ; [Baqir Ali], voix

[Traditions. Asie. Inde. Province de Delhi]

[Archives de la Parole]. , Fable de l'agneau et de la tortue [sic] [i.e. du tigre] : récit en tamoul ; [Prière à la déesse Kunjima] : [chant] / [Sir George Grierson], éd. ; Linguistic survey of India ; Voix parlée et chant, homme

[Traditions. Asie. Inde. Province de Madras [i.e. Chennai]. État du Tamil Nadu]

[Archives de la Parole]. , Durang Kahani : Alope Joma [chant en mundari] / [Sir George Grierson], éd. ; Linguistic survey of India ; Choeur d'hommes, chant. Tuila accompaniment to the mundari marriage song / [Sir George Grierson], éd. ; Linguistic survey of India ; [Guitare monocorde]

[Traditions. Asie. Inde. Chotā Nāgpur]

[Archives de la Parole]. , [Chant en dialecte Toḍa] : chant, dialecte Kasuva [sic], Malabar, Inde / [Sir George Grierson], éd. ; Linguistic survey of India ; Chant solo, homme

[Traditions. Asie. Inde. Province de Madras [i.e. Chennai]]

[Archives de la Parole]. , [Rangami gari] : [chant populaire en dialecte Kui] Inde, Malabar / [Sir George Grierson], éd. ; Linguistic survey of India ; Chant solo, homme

[Traditions. Asie. Inde. Province de Madras [i.e. Chennai]]

[Archives de la Parole]. , [Chant populaire en khāndeśī] : indo-aryen / [Sir George Grierson], éd. ; Linguistic survey of India ; [K.S. Vani], voix

[Traditions. Asie. Inde. État du Maharashtra. Dhule]

[Archives de la Parole]. , [A country girl discards a prince lover] : [chant populaire en sindhī] / [Sir George Grierson], éd. ; Linguistic survey of India ; [Gianchand Santumal], voix

[Traditions. Asie. Inde. Province de Bombay. Région du Haut-Sindh]

[Archives de la Parole]. , [Chant d'opérette en ahīrwāṭī] : "Nautch girl's", indo-aryen / [Sir George Grierson], éd. ; Linguistic survey of India ; [Hassaina], voix ; accompagnement instrumental (harmonium indien et tabla)

[Traditions. Asie. Inde. Province de Delhi. Delhi]

[Archives de la Parole]. , [Parable of the prodigal son] : Récit en hindoustani / [Sir George Grierson], éd. ; Linguistic survey of India ; [Baqir Ali], voix

[Traditions. Asie. Inde. Province de Delhi]

Cross National Contact Strategies

Using paradata gathered from the 11-nation Survey of Health, Ageing and Retirement in Europe (SHARE), this paper examines the impact of the first contact attempt and the first contact properties, respectively, on contact and response efficiency using logistic multilevel models. We find that despite the different sample frames and interviewer compensation structure between countries, there are no considerable country effects with respect to making contact, once interviewer effects are controlled. Moreover, results point to an increased efficiency associated with evenings especially on Sundays, at least on the very first contact attempt. For attempts that result in initial contact, Saturday afternoons are most likely to eventually lead to completed interviews, followed by initial contact on weekdays during the daytime. We hypothesize that this may be due to the SHARE sample being composed of people aged 50 and over.

Inferring the degree of incipient speciation in secondary contact zones of closely related lineages of Palearctic green toads (Bufo viridis subgroup).

Reproductive isolation between lineages is expected to accumulate with divergence time, but the time taken to speciate may strongly vary between different groups of organisms. In anuran amphibians, laboratory crosses can still produce viable hybrid offspring >20 My after separation, but the speed of speciation in closely related anuran lineages under natural conditions is poorly studied. Palearctic green toads (Bufo viridis subgroup) offer an excellent system to address this question, comprising several lineages that arose at different times and form secondary contact zones. Using mitochondrial and nuclear markers, we previously demonstrated that in Sicily, B. siculus and B. balearicus developed advanced reproductive isolation after Plio-Pleistocene divergence (2.6 My, 3.3-1.9), with limited historic mtDNA introgression, scarce nuclear admixture, but low, if any, current gene flow. Here, we study genetic interactions between younger lineages of early Pleistocene divergence (1.9 My, 2.5-1.3) in northeastern Italy (B. balearicus, B. viridis). We find significantly more, asymmetric nuclear and wider, differential mtDNA introgression. The population structure seems to be molded by geographic distance and barriers (rivers), much more than by intrinsic genomic incompatibilities. These differences of hybridization between zones may be partly explained by differences in the duration of previous isolation. Scattered research on other anurans suggests that wide hybrid zones with strong introgression may develop when secondary contacts occur <2 My after divergence, whereas narrower zones with restricted gene flow form when divergence exceeds 3 My. Our study strengthens support for this rule of thumb by comparing lineages with different divergence times within the same radiation.