Electrostatic surface plasmon resonance: Direct electric field-induced hybridization and denaturation in monolayer nucleic acid films and label-free discrimination of base mismatches

We demonstrate that in situ optical surface plasmon resonance spectroscopy can be used to monitor hybridization kinetics for unlabeled DNA in tethered monolayer nucleic acid films on gold in the presence of an applied electrostatic field. The dc field can enhance or retard hybridization and can also denature surface-immobilized DNA duplexes. Discrimination between matched and mismatched hybrids is achieved by simple adjustment of the electrode potential. Although the electric field at the interface is extremely large, the tethered single-stranded DNA thiol probes remain bound and can be reused for subsequent hybridization reactions without loss of efficiency. Only capacitive charging currents are drawn; redox reactions are avoided by maintaining the gold electrode potential within the ideally polarizable region. Because of potential-induced changes in the shape of the surface plasmon resonance curve, we account for the full curve rather than simply the shift in the resonance minimum.

Improving kNN multi-label classification in Prototype Selection scenarios using class proposals

Prototype Selection (PS) algorithms allow a faster Nearest Neighbor classification by keeping only the most profitable prototypes of the training set. In turn, these schemes typically lower the performance accuracy. In this work a new strategy for multi-label classifications tasks is proposed to solve this accuracy drop without the need of using all the training set. For that, given a new instance, the PS algorithm is used as a fast recommender system which retrieves the most likely classes. Then, the actual classification is performed only considering the prototypes from the initial training set belonging to the suggested classes. Results show that this strategy provides a large set of trade-off solutions which fills the gap between PS-based classification efficiency and conventional kNN accuracy. Furthermore, this scheme is not only able to, at best, reach the performance of conventional kNN with barely a third of distances computed, but it does also outperform the latter in noisy scenarios, proving to be a much more robust approach.

Hadhā Dīwān rawḍat al-ʻirfān al-mudhayyalah manẓūmātuhu bi-manthūr al-Durar al-ḥisān [microform] /

Microfilm.

Kitāb al-Laʾāliʾ al-sanīyah fī al-uṣūl al-ḥisābīyah [microform] /

Vol. 2 published by al-Maṭbaʻah al-Bukhārīyah, al-Iskandarīyah.

Isäus der Redner,

Vol. 2: 1 Aufl., 1830.

al-Inshāʾ al-ʻaṣrī lil-kātib wa-al-ḥāsib wa-al-mallāk wa-al-tājir fī al-murāsalāt wa-al-muʻāmalāt wa-al-ḥisāb wa-mask al-dafātir [microform] /

Microfilm.

Disquisitiones criticæ de variis per diversa loca & tempora Bibliorum editionibus. Quibus accedunt castigationes theologi cujusdam parisiensis ad opusculum Isa. Vossii de Sibyllinis oraculis, et ejusdem responsionem ad objectiones nuperæ criticæ sacræ.

Mode of access: Internet.

Code for the measurement of water using standard ISA orifices with free discharge (for use in field testing)

Mode of access: Internet.

Isa. Grangaei ... Commentarii In Aur. Prudentii Clementis libros duos aduersus Symmanchum pro ara victoriae ...

Mode of access: Internet.