Novel Mutation in the ATP-Binding Cassette Transporter A3 (ABCA3) Encoding Gene Causes Respiratory Distress Syndrome in A Term Newborn in Southwest Iran

Introduction: ABCA3 glycoprotein belongs to the ATP-binding cassette (ABC) superfamily of transporters, which utilize the energy derived from hydrolysis of ATP for the translocation of a wide variety of substrates across the plasma membrane. Mutations in the ABCA3 gene are knowingly causative for fatal surfactant deficiency, particularly respiratory distress syndrome (RDS) in term babies. Case Presentation: In this study, Sanger sequencing of the whole ABCA3 gene (NCBI NM_001089) was performed in a neonatal boy with severe RDS. A homozygous mutation has been identified in the patient. Parents were heterozygous for the same missense mutation GGA > AGA at position 202 in exon 6 of the ABCA3 gene (c.604G > A; p.G202R). Furthermore, 70 normal individuals have been analyzed for the mentioned change with negative results. Conclusions: Regarding Human Genome Mutation Database (HGMD) and other literature recherche, the detected change is a novel mutation and has not been reported before. Bioinformatics mutation predicting tools prefer it as pathogenic.

Pediatric Intussusception in Northern Iran: Comparison of Recurrent With Non-Recurrent Cases

Background: Intussusception represents as the invagination of a part of the intestine into itself and is the most common cause of intestinal obstruction in infants and children between 6 months to 3-years-old. Objectives: The objective of this study was to determine the recurrence rate and predisposing factors of recurrent intussusception. Patients and Methods: The medical records of children aged less than 13-years-old with confirmed intussusception who underwent reduction at a tertiary academic care in northern Iran (Mazandran), from 2001 to 2013 were reviewed. Data were extracted and recurrence rate was determined. The two groups were compared by chi square, Fisher, Mann-Whitney and t-test. Diagnosed cases of intussusception consisted of 237 children. Results: Average age of the patients was 19.57 ± 19.43 months with a peak of 3 to 30 months. Male to female ratio was 1.65 and this increased by aging. Recurrence rate was 16% (38 cases). 87 (36.7%) underwent surgery. These were mainly children under one year old. In 71% (40) of episodes recurrence occurred 1 to 7 times within 6 months. The recurrence occurred in 29 (23.5%) children in whom a first reduction was achieved with barium enema (BE) and 5 (5.7%) children who had an operative reduction (P < 0.001) in the first episode. Pathological leading points (PLPs) were observed in 5 cases; 2.6% in recurrence group versus 2% in non-recurrence group (P = 0.91). Three patients had intestinal polyp, 2 patient’s lymphoma and Mackle’s diverticulum. Age (P = 0.77) and sex (P = 0.38) showed no difference between the two groups. PLPs were observed in 1.4% of children aged 3 months to 5 years. This was 13.3%, in older children (P = 0.02). Conclusions: The recurrence of intussusception was related to the method of treatment in the first episode and it was 5-fold higher in children with BE than in operative reduction. Recurrent intussusceptions were not associated with PLPs, they were more idiopathic.

Study on Rotavirus Infection and Its Genotyping in Children Below 5 Years in South West Iran

Background: Human rotaviruses are the most important agents for severe dehydrating diarrhea in children below 5 years old. Rotaviruses (RV) is a serious public health problem in developing and developed countries. Objectives: The aim of this study was to determine the prevalence of rotavirus infection and their genotypes in children younger than 5 years of age with acute diarrhea in Ahvaz, Iran. Materials and Methods: For this study, 200 stool samples from children below 5 years of age with acute diarrhea were collected between October 2011 and March 2012. Initially all stool samples were tested for rotavirus antigen by ELISA, and positive samples were confirmed by RT-PCR targeting the VP6 rotavirus gene. Determination of rotavirus genotypes was carried out by performing RT-PCR for G and P types. Altogether, 15 samples were sequenced. Results: Out of 200 stool samples, 100 (50%) had rotavirus antigen detected by ELISA and 73 (36.5%) were found positive by RT-PCR. Of the rotavirus strains identified, only 63 (86.3%) were positive for both VP7 and VP4 while 10 (13.7%) strains were found nontypeable. Rotavirus infection accounts for 36.5% of gastroenteritis cases in samples from symptomatic children. The most prevalent rotavirus genotypes were G1P [8] (80%) followed by G2P [4] (20%). Conclusions: Our results suggest that group A rotavirus is a major pathogene of acute diarrhea in Ahvaz city. The genotypes circulating are similar with those of other countries.

Molecular detection of Brucella melitensis in sheep and goat milk in Iran

Purpose: To detect Brucella melitensis in the milk of reared sheep and goats from Isfahan and Shahrekord regions, Iran. Methods: A total of 225 milk samples (sheep = 125; goat = 100) were collected from Isfahan and Shahrekord regions, Central Iran. Polymerase chain reaction (PCR) was used to detect the presence of B. melitensis in the milk following standard procedures. Results: From 225 milk samples, 20 (8.9 %) were positive for B. melitensis. Out of 125 sheep milk, 12 (9.6 %) had B. melitensis, and of these, 8 (66.6 %) were milk collected from Shahrekord and 4 (33.3 %) from Isfahan region. On the other hand, out of 100 goat milk samples, 18 (18 %) were positive for B. melitensis, out of which 10 (55.5 %) were from Shahrekord and 8 (44.4 %) from Isfahan. Conclusion: The findings show that B. melitensis is present in a significant proportion of caprine and ovine milk in a section of Iran.

Prevalence of preterm labor in Iran: A systematic review and meta-analysis

Background: Preterm labor, which defines as live-birth delivery before 37 weeks of gestation is a main determinant of neonatal morbidity and mortality around the world. Objective: The aim of this study was to determine the prevalence of preterm labor in Iran by a meta-analysis study, to be as a final measure for policy makers in this field. Materials and Methods: In this meta-analysis, the databases of Thomson database (Web of Knowledge), PubMed/Medline, Science Direct, Scopus, Google Scholar, Iranmedex, Scientific Information Database (SID), Magiran, and Medlib were searched for articles in English and Persian language published between 1995 and 2014. Among the studies with regard to the inclusion and exclusion criteria, 14 studies (out of 1370 publications) were selected. Data were analyzed by using Stata software version 11. The heterogeneity of reported prevalence among studies was evaluated by the Chi-square based Q test and I2 statistics. Results: The results of Chi-square based on Q test and I2 statistics revealed severe heterogeneity (Q=2505.12, p-value < 0.001 and I2= 99.5%) and consequently, the random effect model was used for the meta-analysis. Based on the random effect model, the overall estimated prevalence of preterm in Iran was 9.2% (95% CI: 7.6 – 10.7). Conclusion: Present study summarized the results of previous studies and provided a comprehensive view about the preterm delivery in Iran. In order to achieve a more desirable level and its reduction in the coming years, identifying affecting factor and interventional and preventive actions seem necessary.

Confrontation de l'architecture de l'Iran avec la modernité occidentale : constance de la question identitaire

À la fin du 19e siècle, l’Iran commence à se transformer radicalement. Ce changement est le résultat d’un processus de métamorphose socioculturelle, avec le désir d’effacer les méthodes du passé et de recommencer ; une ambition de faire un renouvellement fondamental dans la société, en bénéficiant des grandes idées progressistes de l'Occident moderne. Cette volonté s’est renforcée à la suite des premières visites en Europe d’étudiants et de Nassereddin Shah, le roi de l'Iran, dans les années 1870. Dans ce contexte, les Iraniens et leurs gouvernants, considérant leurs infériorités politico-économiques, ont décidé de remplacer les frustrations internationales par des idées nationalistes et une propagande de suprématie raciale ou religieuse, notamment concertant « l'identité culturelle ». Suivant ces tentatives pour réformer les infrastructures sociopolitiques de l'Iran, tous les domaines culturels du pays, incluant l’architecture, ont été modifiés, selon les idéologies des dirigeants de l’Iran pendant trois périodes historiques du pays : l'époque Qadjar (dès le règne de Nassereddin Shah en 1848), l'époque Pahlavi (1925-1979) et l'époque Post-révolution islamique (1979- jusqu'à présent). L'idée générale de notre mémoire est d'étudier le processus de modernisation de l'architecture de l'Iran, de même que les influences majeures de tous ces changements, concrétisés par des fusions éclectiques et des idées pluralistes – souvent basées sur la politique. De là, en usant des approches de l’histoire sociale et culturelle de l’art, nous analysons des exemples de monuments de l'architecture publique de l'Iran depuis l'entrée de l'Iran dans la modernité, pour chacune des trois périodes mentionnées. Cela, afin de comprendre si les architectes iraniens ont trouvé de nouvelles conceptions pour opérer un déploiement créatif des principes traditionnels et pour trouver de nouvelles orientations dans le processus général de leur évolution architecturale. Autrement dit, nous cherchons à savoir si l'architecture iranienne, avec tous les changements stylistiques dans le processus de conceptualisation, a pu trouver - depuis l'intervention de la modernité occidentale et de l'architecture moderne - son propre langage de la modernité en architecture.

Confrontation de l'architecture de l'Iran avec la modernité occidentale : constance de la question identitaire

À la fin du 19e siècle, l’Iran commence à se transformer radicalement. Ce changement est le résultat d’un processus de métamorphose socioculturelle, avec le désir d’effacer les méthodes du passé et de recommencer ; une ambition de faire un renouvellement fondamental dans la société, en bénéficiant des grandes idées progressistes de l'Occident moderne. Cette volonté s’est renforcée à la suite des premières visites en Europe d’étudiants et de Nassereddin Shah, le roi de l'Iran, dans les années 1870. Dans ce contexte, les Iraniens et leurs gouvernants, considérant leurs infériorités politico-économiques, ont décidé de remplacer les frustrations internationales par des idées nationalistes et une propagande de suprématie raciale ou religieuse, notamment concertant « l'identité culturelle ». Suivant ces tentatives pour réformer les infrastructures sociopolitiques de l'Iran, tous les domaines culturels du pays, incluant l’architecture, ont été modifiés, selon les idéologies des dirigeants de l’Iran pendant trois périodes historiques du pays : l'époque Qadjar (dès le règne de Nassereddin Shah en 1848), l'époque Pahlavi (1925-1979) et l'époque Post-révolution islamique (1979- jusqu'à présent). L'idée générale de notre mémoire est d'étudier le processus de modernisation de l'architecture de l'Iran, de même que les influences majeures de tous ces changements, concrétisés par des fusions éclectiques et des idées pluralistes – souvent basées sur la politique. De là, en usant des approches de l’histoire sociale et culturelle de l’art, nous analysons des exemples de monuments de l'architecture publique de l'Iran depuis l'entrée de l'Iran dans la modernité, pour chacune des trois périodes mentionnées. Cela, afin de comprendre si les architectes iraniens ont trouvé de nouvelles conceptions pour opérer un déploiement créatif des principes traditionnels et pour trouver de nouvelles orientations dans le processus général de leur évolution architecturale. Autrement dit, nous cherchons à savoir si l'architecture iranienne, avec tous les changements stylistiques dans le processus de conceptualisation, a pu trouver - depuis l'intervention de la modernité occidentale et de l'architecture moderne - son propre langage de la modernité en architecture.

The Effect of Climate Variability on Main Components of Cow Milk in Iran

The main purpose of this study is to assess the relationship between six bioclimatic indices for cattle (temperature humidity (THI), environmental stress (ESI), equivalent temperature (ESI), heat load (HLI), modified heat load (HLInew) and respiratory rate predictor(RRP)) and fundamental milk components (fat, protein, and milk yield) considering uncertainty. The climate parameters used to calculate the climate indices were taken from the NASA-Modern Era Retrospective-Analysis for Research and Applications (NASA-MERRA) reanalysis from 2002 to 2010. Cow milk data were considered for the same period from April to September when cows use natural pasture, with possibility for cows to choose to stay in the barn or to graze on the pasture in the pasturing system. The study is based on a linear regression analysis using correlations as a summarizing diagnostic. Bootstrapping is used to represent uncertainty estimation through resampling in the confidence intervals. To find the relationships between climate indices (THI, ETI, HLI, HLInew, ESI and RRP) and main components of cow milk (fat, protein and yield), multiple liner regression is applied. The least absolute shrinkage selection operator (LASSO) and the Akaike information criterion (AIC) techniques are applied to select the best model for milk predictands with the smallest number of climate predictors. Cross validation is used to avoid over-fitting. Based on results of investigation the effect of heat stress indices on milk compounds separately, we suggest the use of ESI and RRP in the summer and ESI in the spring. THI and HLInew are suggested for fat content and HLInew also is suggested for protein content in the spring season. The best linear models are found in spring between milk yield as predictands and THI, ESI,HLI, ETI and RRP as predictors with p-value < 0.001 and R2 0.50, 0.49. In summer, milk yield with independent variables of THI, ETI and ESI show the highest relation (p-value < 0.001) with R2 (0.69). For fat and protein the results are only marginal. It is strongly suggested that new and significant indices are needed to control critical heat stress conditions that consider more predictors of the effect of climate variability on animal products, such as sunshine duration, quality of pasture, the number of days of stress (NDS), the color of skin with attention to large black spots, and categorical predictors such as breed, welfare facility, and management system. This methodology is suggested for studies investigating the impacts of climate variability/change on food quality/security, animal science and agriculture using short term data considering uncertainty or data collection is expensive, difficult, or data with gaps.

La relazione cinematografica tra Italia e Iran

Questa tesi illustra la presenza del cinema italiano in Iran e analizza come gli spettatori abbiano accettato il cinema straniero in concomitanza con le trasformazioni della società iraniana. L’indagine parte dagli aspetti economici derivati dal petrolio come motore di crescita e cambiamento della società iraniana. Elemento imprescindibile dai rapporti diplomatici con i paesi occidentali, la tesi ripercorre le tappe della nazionalizzazione petrolifera, analizza il ruolo giocato dall’Italia in questo contesto e la nascita del rapporto commerciale tra Italia e Iran. Lo studio prende poi in esame più nel dettaglio le relazioni italo-iraniane dal punto di vista cinematografico, e il successo dei film italiani in Iran. Pertanto, la tesi illustra le motivazioni della presenza cinematografica italiana in Iran e prende in esame l’influenza prodottasi attraverso le relazioni bilaterali. L’analisi attraversa le tappe del doppiaggio cinematografico in lingua persiana effettuato in Italia, il cinema popolare iraniano e il cinema intellettuale. Fermo restando una componente italiana nello studio, maggiore attenzione è dedicata al Paese d’arrivo, l’Iran, usando dati tratti da documenti storici, testi, giornali, risorse disponibili e interviste con persone informate in materia. Lo studio è concentrato su testi che approfondiscono la situazione economica e cinematografica iraniana all’epoca della monarchia di Mohammad Reza Pahlavi, coincidente con il secondo dopoguerra, durante il quale l’influenza cinematografica italiana si è fatta maggiormente sentire sul cinema iraniano. Obiettivo della tesi è definire le ragioni che hanno favorito lo sviluppo del rapporto cinematografico tra i due paesi, come il cinema italiano si sia manifestato nel cinema iraniano, e i presupposti che hanno comportato la crescita di quest’ultimo.

Les machines du pouvoir. Technique et politique entre l'Iran sassanide et le califat abbasside

Dans l’Antiquité, la recherche sur la technique permet les premières réalisations de dispositifs ingénieux, tels que des appareils qui accomplissent une série d’actions par le biais de stimulus externes et de mécanismes cachés. Les organismes politiques et religieux saisissent rapidement la puissance communicative de ces machines, en devenant les promoteurs et patrons privilégiés de leur production. L’Empire sassanide (224-650) ne constitue pas une exception. En effet, les souverains perses consacrent, au moins à l’époque tardive, une grande attention à la conception et au déploiement de dispositifs savants. De même, un siècle plus tard, dans le milieu du califat islamique, les Abbassides (750-1258) semblent s’entourer de tels dispositifs. La continuité entre les deux empires dans plusieurs domaines, de la théorie politique à l’administration, est bien connue. Cependant, la question de la réutilisation du patrimoine technique et scientifique ancien, et notamment sassanide, par la cour abbasside, demeure encore largement inexplorée. L’étude d’un corpus de sources, aussi vaste qu’hétérogène, rassemblant des ouvrages historiographiques, géographiques, poétiques et d’adab, ainsi que des traités scientifiques et techniques en plusieurs langues, permet d’analyser différents aspects de la production et de l’usage politique des machines. Au sein de la cour sassanide, comme de la cour abbasside, la machine s’avère constituer un véhicule préférentiel de représentation et de diffusion de l’idéologie politique. À travers sa mise en scène publique, elle contribue de manière substantielle à la définition de l’espace du pouvoir, en participant à la création d’une image de la cour comme un microcosme au cœur duquel le Roi des rois, et plus tard le calife, occupaient le rôle cardinal de maître incontesté du monde. La continuité entre les empires sassanide et abbasside dans le domaine technique ne se limite donc pas à une récupération de savoirs, mais s’opère aussi sous la forme d’une véritable réactivation d’un patrimoine symbolique

Chemoprotection Of Mnng-initiated Gastric Cancer In Rats Using Iranian Propolis.

Iranian propolis is a natural product of honeybees that has significant and varied anti-cancer benefits. The present study was designed to investigate the protective effects of Iranian propolis on gastric tissue carcinogenesis in an animal model.  Propolis samples were collected from Hamadan and Taleghan districts of Iran, followed by ultra performance liquid chromatography mass spectrometry analysis. Fifty-five rats were divided into three groups; control, Taleghan propolis and Hamadan propolis. All the animals received N-methyl-N-nitro-N-nitrosoguanidine (MNNG, 100 μg/ml) in drinking water ad libitum for 34 weeks. In the treated groups, nutrition with propolis was started two weeks before MNNG administration. At the end of the study, the entire gastrointestinal tract was scrutinized for tumors, and the rest of the body was assessed for metastatic deposits.  Results indicated that the incidence and number of tumors were significantly decreased by propolis in comparison with the control group (P < 0.05). The nuclear/cytoplasmic ratio, epithelial stratification, nuclear dispolarity, structural abnormality, and Beta-catenin and Bcl-2 proteins expression were significantly reduced in the propolis group compared to the control group (P < 0.05). In addition, Bax protein expression was significantly increased in the propolis group in comparison with the control group (P < 0.05).  The present study demonstrated the potential chemoprotective effects of the Iranian propolis against gastric cancer in a typical animal model. The results provide evidence for the hypothesis that Iranian propolis may exert a chemoprotective effect on MNNG-initiated gastric cancer through inhibition of cell proliferation and apoptosis induction.

Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus

We present the genome sequences of a new clinical isolate of the important human pathogen, Aspergillus fumigatus, A1163, and two closely related but rarely pathogenic species, Neosartorya fischeri NRRL181 and Aspergillus clavatus NRRL1. Comparative genomic analysis of A1163 with the recently sequenced A. fumigatus isolate Af293 has identified core, variable and up to 2% unique genes in each genome. While the core genes are 99.8% identical at the nucleotide level, identity for variable genes can be as low 40%. The most divergent loci appear to contain heterokaryon incompatibility ( het) genes associated with fungal programmed cell death such as developmental regulator rosA. Cross-species comparison has revealed that 8.5%, 13.5% and 12.6%, respectively, of A. fumigatus, N. fischeri and A. clavatus genes are species-specific. These genes are significantly smaller in size than core genes, contain fewer exons and exhibit a subtelomeric bias. Most of them cluster together in 13 chromosomal islands, which are enriched for pseudogenes, transposons and other repetitive elements. At least 20% of A. fumigatus-specific genes appear to be functional and involved in carbohydrate and chitin catabolism, transport, detoxification, secondary metabolism and other functions that may facilitate the adaptation to heterogeneous environments such as soil or a mammalian host. Contrary to what was suggested previously, their origin cannot be attributed to horizontal gene transfer ( HGT), but instead is likely to involve duplication, diversification and differential gene loss (DDL). The role of duplication in the origin of lineage-specific genes is further underlined by the discovery of genomic islands that seem to function as designated ""gene dumps'' and, perhaps, simultaneously, as "" gene factories''.

Identification of possible targets of the Aspergillus fumigatus CRZ1 homologue, CrzA

Background: Calcineurin, a serine/threonine-specific protein phosphatase, plays an important role in the control of cell morphology and virulence in fungi. Calcineurin regulates localization and activity of a transcription factor called CRZ1. Recently, we characterize Aspergillus fumigatus CRZ1 homologue, AfCrzA. Here, we investigate which pathways are influenced by A. fumigatus AfCrzA during a short pulse of calcium by comparatively determining the transcriptional profile of A. fumigatus wild type and.AfcrzA mutant strains. Results: We were able to observe 3,622 genes modulated in at least one timepoint in the mutant when compared to the wild type strain (3,211 and 411 at 10 and 30 minutes, respectively). Decreased mRNA abundance in the Delta crzA was seen for genes encoding calcium transporters, transcription factors and genes that could be directly or indirectly involved in calcium metabolism. Increased mRNA accumulation was observed for some genes encoding proteins involved in stress response. AfCrzA overexpression in A. fumigatus increases the expression of several of these genes. The deleted strain of one of these genes, AfRcnA, belonging to a class of endogenous calcineurin regulators, calcipressins, had more calcineurin activity after exposure to calcium and was less sensitive to menadione 30 mu M, hydrogen peroxide 2.5 mM, EGTA 25 mM, and MnCl(2) 25 mM. We constructed deletion, overexpression, and GFP fusion protein for the closely related A. nidulans AnRcnA. GFP

A reliable measure of similarity based on dependency for short time series: an application to gene expression networks

Background: Microarray techniques have become an important tool to the investigation of genetic relationships and the assignment of different phenotypes. Since microarrays are still very expensive, most of the experiments are performed with small samples. This paper introduces a method to quantify dependency between data series composed of few sample points. The method is used to construct gene co-expression subnetworks of highly significant edges. Results: The results shown here are for an adapted subset of a Saccharomyces cerevisiae gene expression data set with low temporal resolution and poor statistics. The method reveals common transcription factors with a high confidence level and allows the construction of subnetworks with high biological relevance that reveals characteristic features of the processes driving the organism adaptations to specific environmental conditions. Conclusion: Our method allows a reliable and sophisticated analysis of microarray data even under severe constraints. The utilization of systems biology improves the biologists ability to elucidate the mechanisms underlying celular processes and to formulate new hypotheses.

A correct enthalpy relationship as thermal comfort index for livestock

Researchers working with thermal comfort have been using enthalpy to measure thermal energy inside rural facilities, establishing indicator values for many situations of thermal comfort and heat stress. This variable turned out to be helpful in analyzing thermal exchange in livestock systems. The animals are exposed to an environment which is decisive for the thermoregulatory process, and, consequently, the reactions reflect states of thermal comfort or heat stress, the last being responsable for problems of sanity, behavior and productivity. There are researchers using enthalpy as a qualitative indicator of thermal environment of livestock such as poultry, cattle and hogs in tropical regions. This preliminary work intends to check different enthalpy equations using information from classical thermodynamics, and proposes a direct equation as thermal comfort index for livestock systems.