Ultrastructural description of Ceratomyxa microlepis sp. nov. (Phylum Myxozoa): a parasite infecting the gall bladder of Hemiodus microlepis, a freshwater teleost from the Amazon River

A new ceratomyxid parasite was examined for taxonomic identification, upon being found infecting the gall bladder of Hemiodus microlepis (Teleostei: Hemiodontidae), a freshwater teleost collected from the Amazon River, Brazil. Light and transmission electron microscopy revealed elongated crescent-shaped spores constituted by two asymmetrical shell valves united along a straight sutural line, each possessing a lateral projection. The spores body measured 5.2 ± 0.4 µm (n = 25) in length and 35.5 ± 0.9 µm (n = 25) in total thickness. The lateral projections were asymmetric, one measuring 18.1 ± 0.5 µm (n = 25) in thickness and the other measuring 17.5 ± 0.5 µm (n = 25) in thickness. Two equal-sized subspherical polar capsules measuring 2.2 ± 0.3 µm in diameter were located at the same level, each possessing a polar filament with 5-6 coils. The sporoplasm was binucleate. Considering the morphometric data analyzed from the microscopic observations, as well as the host species and its geographical location, this paper describes a new myxosporean species, herein named Ceratomyxa microlepis sp. nov.; therefore representing the first description of a freshwater ceratomyxid from the South American region.

Light and electron microscopy of Myxobolus sciades n. sp. (Myxozoa), a parasite of the gills of the Brazilian fish Sciades herzbergii (Block, 1794) (Teleostei: Ariidae)

A myxosporean parasite in the gill lamellae of the freshwater teleost fish, Sciades herzbergii (Ariidae) (Block, 1794), from the Poti River (Northeast of Brazil) was described by light and electron microscopy studies. Polysporic histozoic cyst-like plasmodia containing several life-cycle stages, including mature spores, were observed. The spores were pyriform and uninucleate, measuring 9.15 ± 0.39 μm (n = 50) long, 4.36 ± 0.23 μm (n = 25) wide and 2.61 ± 0.31 μm (n = 25) thick. Elongated pyriform polar capsules (PC) were of equal size (4.44 ± 0.41 μm long and 1.41 ± 0.42 μm in diameter) and each contained a polar filament with 9-10 coils obliquely arranged in relation to the axis of PC. The PC wall was composed of two layers of different electron densities. Histological analysis revealed the close contact of the cyst-like plasmodia with the basal portion of the epithelial gill layer, which exhibited some alterations in the capillary vessels. Based on the morphological and ultrastructural differences, the similarity of the spore features to those of the genus Myxobolus and the specificity of this host to previously described species, we describe a new species named Myxobolus sciades n. sp. in this study.

Avaliação do viés GC em plataformas de sequenciamento de nova geração

O surgimento das plataformas de sequenciamento de nova geração (NGS) proporcionou o aumento do volume de dados produzidos, tornando possível a obtenção de genomas completos. Apesar das vantagens alcançadas com estas plataformas, são observadas regiões de elevada ou baixa cobertura, em relação à média, associadas diretamente ao conteúdo GC. Este viés GC pode afetar análises genômicas e dificultar a montagem de genomas através da abordagem de novo, além de afetar as análises baseadas em referência. Além do que, as maneiras de avaliar o viés GC deve ser adequada para dados com diferentes perfis de relação/associação entre GC e cobertura, tais como linear e quadrático. Desta forma, este trabalho propõe o uso do Coeficiente de Correlação de Pearson (r) para analisar a correlação entre conteúdo GC e Cobertura, permitindo identificar aintensidade da correlação linear e detectar associações não-lineares, além de identificar a relação entre viés GC e as plataformas de sequenciamento. Os sinais positivos e negativos de r também permitem inferir relações diretamente proporcionais e inversamente proporcionais respectivamente. Utilizou-se dados da espécie Corynebacterium pseudotuberculosis, conhecido por serem genomas clonais obtidas através de diferentes tecnologias de sequenciamento para identificar se há relação do viés GC com as plataformas utilizadas.

Proposta de uma metodologia para obtenção de um gerador síncrono com rotor de ímãs permanentes inclinados axialmente para microcentrais elétrica a partir de motor de indução

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Endophytic fungi producing of esterases: evaluation in vitro of the enzymatic activity using pH indicator

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análise de formas planas em imagens digitais: uma nova abordagem baseada na Transformada de Hough

Pós-graduação em Ciência da Computação - IBILCE

Phylogeny, ultrastructure and histopathology of Myxobolus lomi sp nov., a parasite of Prochilodus lineatus (Valenciennes, 1836) (Characiformes: Prochilodontidae) from the Peixes River, Sao Paulo State, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Identification of two novel cytolysins from the hydrozoan Olindias sambaquiensis (Cnidaria)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Study of magnetic field enhanced plasma immersion ion implantation in Silicon

A comparison between experimental measurements and numerical calculations of the ion current distribution in plasma immersion ion implantation (PIII) with external magnetic field is presented. Later, Silicon samples were implanted with nitrogen ion to analyze the effect on them. The magnetic field considered is essentially non-uniform and is generated by two magnetic coils installed on vacuum chamber. The presence of both, electric and magnetic field in PIII create a crossed ExB field system, promoting drift velocity of the plasma around the target. The results found shows that magnetized electrons drifting in ExB field provide electron-neutral collision. The efficient ionization increases the plasma density around the target where a magnetic confinement is formed. As result, the ion current density increases, promoting significant changes in the samples surface properties, especially in the surface wettability.

Narrow Fungal Mycorrhizal Diversity in a Population of the Orchid Coppensia doniana

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Desenvolvimento, otimização e caracterização física de um novo sistema multi-sensores de Biosusceptometria de Corrente Alternada (BAC) para obtenção de imagens biomagnéticas

A novel AC Biosusceptometry (ACB) system with thirteen sensors it was implemented and characterized in vitro using magnetic phantoms. The system presenting coils in a coaxial arrangement with one pair of excitation coil outside and thirteen pairs of detection coils inside. A first-order gradiometric configuration was utilized for optimal detection of magnetic signals. Several physical parameters such as baseline, number of turns, excitation field and diameters were studied for improvement of the signal/noise ratio. This system exhibits an enhanced sensitivity and spatial resolution, due to the higher density of sensors/area. In the future those characteristics will turn possible to obtain images of magnetic marker or tracer in the gastrointestinal tract focusing on physiological and pharmaceutical studies. ACB emerged due to its interesting nature, noninvasiveness and low cost to investigate gastrointestinal parameters and this system can contribute for more accurate interpretation of biomedical signals and images

Comparação da razão sinal-ruído e da uniformidade da imagem entre aquisições com bobina de quadratura e com bobina de corpo

The signal-to-noise ratio and image uniformity analysis parameters are very important in quality control of an MRI scanner. They are measured in regular tests with phantoms. In these tests, however, used to quadrature coil, which has been most widely used clinically, and, therefore, was replaced in the procedures for body coil. In order to understand the difference between these two parameters in these coils, the study aimed to analyze the images acquired from four different phantoms in the same equipment under the same conditions for comparison purposes. With these results, it can be concluded that the body coil signal-to-noise ratio has always smaller than the quadrature in any projection, whereas the image uniformity is larger

Quantificação da desintegração de comprimidos por fluxo ótico em seqüências de imagens de biosusceptometria AC

AC Biosusceptometry (ACB) is a low-cost biomagnetic tool that has been successfully applied on pharmaceutical research to evaluate performance of solid dosage forms. The aim of this work was to evaluate the Horn & Shunck method to access tablet disintegration. To evaluate the HS results was record on video a test with a objet moving in a rail with a constant velocity. The desintegration was recorded on video and ACB, which used have seven pairs of detection coils and a pair of excitation coils to mensure the magnetic ux variation. The signals were ampli ed and digitalized to create images, which were restored by Wiener lter, while the video images are converted to gray scale, both are normalized and binarized and had the optical ow estimation calculated by Horn & Schunck (HS) algorithm. All signals and images are processed and developed algorithm on Matlab. During the tests the ve tablets (500mg ferrite, 375mg excipients, compression 10 to 50 kN) were on a becker between of the ACB system and of the video system, and only touching the surface of the water. With all OF maps calculated was realized the sum of the resultants of each, to get a disintegration process resultant for each compression. Whit that was possible observed the disintegration behaves. For the compression force study the HS components of each sequence was sum, take mean and normalized for sequence's max modulo, therefore can be observed a high growing on less compression tablets. We can conclude the HS algorithm is viable to tablets disintegration data collection and whit that was possible to create a tablets disintegration analyzes protocol, which would be useful on desintegration kinetics study

Projeto de um dispositivo para prensar bobinas polares de pólos salientes para hidrogeradores

The increasing demand for productivity and quality in companies has converged to a common point: reducing costs. In this context the present work aims at the development of a mechanical press which is designed for pressing polar hydrogenerators coils with salient pole in field facilitating the assembly of the poles in the plant, as well as reforms especially in hydrogenerators, reducing significantly the transport costs. With security in mind as well as reduced costs, a study was made of the materials to be used as it was applied a methodology for calculating the correct choice of safety factor to be used in the device. Through mechanical calculations were dimensioned critical items of the device as the diameter of the rods as well as the minimum thickness of the base of the device must have so that it does not break threaded shear in the region by applying the total load of traction on the risers implementation of the pressing. All compression loading device will be through the application of torque on the nuts of bolts in this way was defined by calculations the required torque for each nut so that you can reach the pressure specified in the design specifies. The modeling of the device was made using the INVENTOR™ program in conjunction with the program ANSYS ™. These programs have created designs in three dimensions, assembly and simulation of stress analysis in components of the device

Estudo de uma ponte rolante comercial no transportede bobina de aço de 12 toneladas

This work aims to determine the stresses acting on the main beam of a crane to transport steel coils of up to twelve tons. To determine the stress it was made a revision of the knowledge of the mechanics of materials to apply the analytical method. Following a review of the finite element method is made to understand the same. To complete the study it was used the commercial software ANSYS to determine the stresses by finite element method, the program provides images that help to better understand the results obtained. With the results a comparison of the values of the tensions between the two methods (analytical and finite element) was made. To assist in the calculations it was used the NBR 8400, 1984 (Calculation of Lifting Equipment Load)