308 resultados para Fita cassete


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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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The routine semen evaluation assessing sperm concentration, motility and morphology, does not identify subtle defects in sperm chromatin architecture. Bulls appear to have stable chromatin, with low levels of DNA fragmentation. However, the nature of fragmentation and its impact on fertility remain unclear and there are no detailed reports characterizing the DNA organization and damage in this species. The intensive genetic selection, the use of artificial insemination and in vitro embryo production associated to the cryopreservation process can contribute to the chromatin damage and highlights the importance of sperm DNA integrity for the success of these technologies. Frozen-thawed semen samples from three ejaculates from a Nellore bull showed high levels of morphological sperm abnormalities (55.8±5.1%), and were selected for complementary tests. Damage of acrosomal (76.9±8.9%) and plasma membranes (75.7±9.3%) as well as sperm DNA strand breaks (13.8±9.5%) and protamination deficiency (3.7±0.6%) were significantly higher compared to the values measured in the semen of five Nellore bulls with normospermia (24.3±3.3%; 24.5±6.1%; 0.6±0.5%; 0.4±0.6% for acrosome, plasma membrane, DNA breaks and protamine deficiency, respectively) (P<0.05). Motility and percentage of spermatozoa with low mitochondrial potential showed no differences between groups. This study shows how routine semen analyses (in this case morphology) may point to the length and complexity of sperm cell damage emphasizing the importance of sperm function testing.

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Pós-graduação em Biociências e Biotecnologia Aplicadas à Farmácia - FCFAR

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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There is great genetic diversity of rambutan plants, thus, to establish commercial orchards culture, it is recommended the use of plants vegetatively propagated. Therefore, this research aimed to determine the best method of grafting, graft protection and type of rootstock for its propagation. The experiment was repeated in two seasons (autumn/winter and spring/summer). The experimental design was completely randomized in both experiments, with four replicates, each experimental unit consisted of 10 plants analyzed in a 4x2x2 factorial with four types of grafting (whip graft-WG; cleft graft -CG; wedge graft-WG; and inverted wedge graft-IWG;), 2 types of graft protection (Biodegradable and plastic) and 2 types of rootstock (without or with leaves). The variables analyzed in this study were: percentage of grafting success, number and length of buds (cm). For grafting performed in autumn /winter, both by cleft grafting as whip graft with biodegradable strip and rootstock leafless provided the best results in propagation of rambutan tree. The best results were observed during the autumn / winter.

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Pós-graduação em Agronomia (Energia na Agricultura) - FCA

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Agronomia (Ciência do Solo) - FCAV

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)