Avaliação espaço-temporal do zooplâncton da laguna estuarina de Guaraíras (RN, Brasil)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Biomonitoramento da comunidade de macroinvertebrados de um reservatório de abastecimento público no sul do Brasil

The faunal inventory of the macroinvertebrate community is important to the environmental assessment, since this biota is sensitive to human disturbance. The reservoir of Rio Verde, located on the first plateau of Paraná, is inserted into an agricultural region with several forest fragments Araucaria. The aim of this study was to evaluate the environmental integrity of the reservoir through ecological indexes of macroinvertebrate community benthic and associated with macrophytes. Five sampling points were defined in the study area, which comprise distinct microhabitats in the basin. There were four sampling campaigns, each by weather station: Spring (2014); Summer (2015); Autumn (2015) and Winter (2015). In each sample were measured abiotic various parameters in the field and be collected water samples for nutrient analysis in the laboratory. The macroinvertebrates were collected in triplicate at adapted Macan method using mesh sieve 1 mm and CPUE (catch per unit effort) for 20 minutes. In order to pellet sample was used a dredger model Petersen 2L. Still in the field, by season, samples were collected from macrophytes Myriophyllum aquaticum (Vell) Verdc. and Potamogeton montevidensis A. Benn. in triplicates in the fluvial region of the reservoir, to analyze the associated fractal dimension and macrofauna. For this we used a PVC sampler specific volume 0.025 m3. the following ecological descriptors were calculated in each case: abundance, wealth tax, wealth Margalef, Shannon-Wiener diversity, evenness evenness through the Past software. The index Biological Monitoring Working Party (BMWP) for monitoring sampling points was also calculated. Regarding the statistical analysis, we used the analysis of PERMANOVA to compare points and seasons and canonical correspondence analysis (CCoA) for variables. Regarding M. aquaticum and P. montevidensis it was not verified difference to the average associated macroinvertebrates. However there was a difference for abundance of organisms in the fractal dimension and biomass of specimens. M. aquaticum is more complex and took more macrofauna in relation to P. montevidensis. Regarding the monitoring of the reservoir, it showed up mesotrophic with moderate nutrient concentrations and within the regulatory limits. Benthic macrofauna showed statistical differences in relation to the reservoir region, sample point and temporal variation. The BMWP index showed that the river region has the highest biotic integrity (in all samples above 70 points), and the ecological descriptors of wealth and Margalef diversity of Shannon- Wiener higher. In point 4 (dam downstream) were recorded evidence of possible impacts due to lower wealth and BMWP index which resulted in a questionable quality water. New approaches are needed to focus on the aquatic community in the best understanding of this ecosystem and also with a view to environmental preservation of the Green River Basin.

Análise metagenômica da microbiota de ambientes aquáticos do estado do Rio Grande do Norte - Brasil

The screening for genes in metagenomic libraries from soil creates opportunities to explore the enormous genetic and metabolic diversity of microorganisms. Rivers are ecosystems with high biological diversity, but few were examined using the metagenomic approach. With this objective, a metagenomic library was constructed from DNA soil samples collected at three different points along the Jundiaí-river (Rio Grande do Norte-Brazil). The points sampled are from open area, rough terrain and with the direct incidence of sunlight. This library was analyzed functionally and based in sequence. For functional analysis Luria-Bertani solid medium (LB) with NaCl concentration varied from 0.17M to 0.85M was used for functional analysis. Positives clones resistant to hypersaline medium were obtained. The recombinant DNAs were extracted and transformed into Escherichia coli strain DH10B and survival curves were obtained for quantification of abiotic stress resistance. The sequences of clones were obtained and submitted to the BLASTX tool. Some clones were found to hypothetical proteins of microorganisms from both Archaea and Bacteria division. One of the clones showed a complete ORF with high similarity to glucose-6-phosphate isomerase which participates in the synthesis of glycerol pathway and serves as a compatible solute to balance the osmotic pressure inside and outside of cells. Subsequently, in order to identify genes encoding osmolytes or enzymes related halotolerance, environmental DNA samples from the river soil, from the water column of the estuary and ocean were collected and pyrosequenced. Sequences of osmolytes and enzymes of different microorganisms were obtained from the UniProt and used as RefSeqs for homology identification (TBLASTN) in metagenomic databases. The sequences were submitted to HMMER for the functional domains identification. Some enzymes were identified: alpha-trehalose-phosphate synthase, L-ectoina synthase (EctC), transaminase L-2 ,4-diaminobutyric acid (EctB), L-2 ,4-diaminobutyric acetyltransferase (EctA), L-threonine 3 dehydrogenase (sorbitol pathway), glycerol-3-phosphate dehydrogenase, inositol 3-phosphate dehydrogenase, chaperones, L-proline, glycine betaine binding ABC transporter, myo-inositol-1-phosphate synthase protein of proline simportadora / PutP sodium-and trehalose-6-phosphate phosphatase These proteins are commonly related to saline environments, however the identification of them in river environment is justified by the high salt concentration in the soil during prolonged dry seasons this river. Regarding the richness of the microbiota the river substrate has an abundance of halobacteria similar to the sea and more than the estuary. These data confirm the existence of a specialized response against salt stress by microorganisms in the environment of the Jundiaí river

Avaliação da temperatura média prevista pelos modelos climáticos globais no sudeste do Brasil.

Os modelos climáticos globais são considerados a melhor ferramenta para gerar cenários prováveis de alterações climáticas futuras, apesar das incertezas. As projeções são diferentes para cada região e variam ao longo das estações do ano. O objetivo deste trabalho foi avaliar a similaridade entre projeções dos modelos climáticos globais do IPCC-AR4 para temperatura média na região Sudeste do Brasil, no período de 2071 a 2100, cenário A2. Para tanto, foram realizadas análise de componentes principais e análise de agrupamento hierárquico para agrupar os modelos de comportamentos semelhantes. Adotando-se quatro grupos de modelos, os valores médios de temperatura variaram de 23,7°C a 25,4°C.

Contribuição de uma seção gravimétrica transversal ao estudo da estruturação litosférica na porção setentrional da Província Borborema, NE do Brasil

Este trabalho apresenta um modelo gravimétrico 2,5D gerado a partir de 315 novas estações gravimétricas levantadas ao longo de uma seção transversal NW-SE com 750 km de extensão na porção setentrional da Província Borborema, NE do Brasil. A modelagem gravimétrica foi aplicada separadamente nas componentes regional e residual do campo gravitacional. A modelagem 2,5D das anomalias regionais revelou que a profundidade da interface crosta-manto varia de 28 a 32 km, considerando uma densidade média de 2,8 g/cm3 para a crosta continental e de 3,3 g/cm3 para o manto litosférico. As anomalias residuais de alta frequência foram interpretadas a partir do contraste de densidade da crosta superior, com uma espessura não superior a 10 km, e uma ampla associação litológica, com densidades variando de 2,55 a 2,9 g/cm3. A configuração geotectônica litosférica atual da Província Borborema é claramente resultado da ruptura dos continentes Sul-Americano e Africano no Mesozoico, na qual boa parte dos vestígios das estruturas tectônicas de grandes profundidades formadas durante a orogênese Brasiliana/Pan-Africana foi mascarada pelo último episódio tectônico responsável pela fragmentação do Gondwana Ocidental. _______________________________________________________________________________________ ABSTRACT

Diversidade de macroalgas marinhas bentônicas dos recifes de Maracajaú, área de preservação ambiental dos recifes de corais, Rio Grande do Norte, Brasil

Despite the importance of coral reefs to humanity, these environments have been threatened throughout the world. Several factors contribute to the degradation of these ecosystems. The Maracajaú Reef Complex, in Rio Grande do Norte state is part of the Coral Reefs Environment Preservation Area in northeastern Brazil. This area has been receiving an increasing influx of tourism and the integrity of the local reefs is a matter of concern. In this study, the reef macroalgae communities were studied and compared within two areas distinguished by the presence or absence of tourism activities. Two sample sites were chosen: the first one, where diving activities are intense; and the other, where these practices do not occur. Samples were collected at both sites within a quadrate of 625 cm2 of area randomly thrown 5 times along a 10 meters transect line. Richness, Shannon-Hill diversity and Simpson dominance indices were determined based on biomass data. Similarity between sites was analyzed with Bray-Curtis similarity and distance index. Fifty-eight macroalgae species were observed, including 7 Chlorophyta, 13 Phaeophyta and 38 Rhodophyta. In the non-disturbed site, 49 species were found, while at the disturbed site, there were 42 species. Dictyotaceae and Corallinaceae were the most representative families at the non-disturbed site, and Rhodomelaceae and Dictyotaceae at the disturbed site. The non-disturbed site presented a higher biomass and the greatest richness and diversity indices. In the disturbed site the dominance index was greater and Caulerpa racemosa was the dominant species. The dendogram based on similarity index showed two major clusters, and an isolated element at the center that corresponds to a sample from the disturbed site. In the first cluster, samples from the non-impacted site were predominant and fleshy brown algae were more conspicuous. The second cluster was composed primarily of samples from the impacted site, where C. racemosa and red filamentous and erect calcareous algae associations (turf forming) were observed covering large extensions. These associations are represented by groups of algae adapted to environments where disturbances are frequent. They can grow rapidly on substrate where benthic community was removed and do not allow the establishment of other species. The results of the present study show that tourism activity is an impacting factor that has been causing shifts in macroalgae communities in the Maracajaú Reef Complex

Avaliação espaço-temporal do zooplâncton da laguna estuarina de Guaraíras (RN, Brasil)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

DINÂMICA DA OCUPAÇÃO E UTILIZAÇÃO DO ESPAÇO EM COMUNIDADES DO PARANÁ DO ARANAPÚ, NA RESERVA DE DESENVOLVIMENTO SUSTENTÁVEL MAMIRAUÁ-AM/ BRASIL

A pesquisa objetivou fazer uma reflexão acerca da ocupação e utilização do espaço de comunidades localizadas no Rio Aranapú, na Reserva de Desenvolvimento Sustentável Mamirauá. Esta RDS está situada na confluência dos rios Solimões, Japurá e Auti-Paraná, no Médio Solimões, com extensão de 1.124.000 ha. Constitui-se na primeira experiência brasileira em implantar em área de várzea, um conjunto de ações que objetivam conciliar a conservação da biodiversidade com a melhoria da qualidade de vida das populações que habitam a área. Por estarem localizadas em ambiente de várzea, essas comunidades adaptam seu modo de vida às modificações da natureza, ou seja, à subida e descida do nível da água do rio, a cada seis meses. A pesquisa está pautada numa discussão que remete ao entendimento da dinâmica de ocupação do espaço de acordo com as duas estações bem definidas na região: cheia e seca. Para isso utiliza-se de abordagem geográfica referente ao espaço geográfico, visto que a produção ocorre conforme o desenvolvimento de tais áreas de ocupação. Baseia-se nos referenciais nacionais e internacionais da Educação Ambiental para a gestão participativa, uma vez que entre os fatores mais significativos para fixação das comunidades nessa região está a presença abundante de recursos naturais.

Relação entre altitude e temperatura: uma contribuição ao zoneamento climático no estado de Santa Catarina, Brasil.

A relação da altitude com a temperatura é especialmente importante para as regiões tropicais e subtropicais onde uma diferença altitudinal de algumas centenas de metros provoca mudanças sensíveis no ambiente, adaptação da biota e consequente sucesso na introdução de espécies para cultivo agrícola. A temperatura do ar sofre alterações com a altitude, latitude e longitude e em função do relevo cada local pode apresentar um gradiente térmico específico. Neste trabalho, o Estado de Santa Catarina foi delimitado em grupos climáticos homogêneos e se estabeleceram análises de correlação entre a temperatura média de janeiro, de julho e média anual, com as respectivas altitudes dos grupos assim formados. A correlação da altitude com a temperatura média anual de janeiro, considerando dados de 44 estações meteorológicas, foi mais forte comparada à temperatura média de julho. Para os coeficientes de correlação mais elevados foram obtidas retas de regressão linear simples e os respectivos coeficientes de determinação das retas. O gradiente térmico médio obtido para o conjunto de todas as estações do Estado foi de -1ºC/213m, o que equivale a uma redução de aproximadamente 0,48oC a cada 100 metros de altitude. Foram também obtidos gradientes térmicos médios em função da latitude e longitude. A altitude, latitude e longitude, nesta ordem, influenciam a temperatura média do ar.

Homogeneidade e heterogeneidade nos sistemas educacionais: Argentina, Brasil, Chile e México

This study focus on the reconfiguration of educational management in Argentina, Brazil, Chile and Mexico, which was promoted by the new logic of social regulation and the new role attributed to the State, considering each country's own historicity. So, the cultural factors that interfere on the dynamics of the 90' school reform are analyzed. Aspects that show the homogeneity or heterogeneity of these reforms in the region, as well as local specificities that block out the concretization of the reform are underlined. It is shown that the historicity that characterizes the educational reform has taken, in each country, a form that can be called, in Mexico, conservative rupture; in Chile, conservative continuity; in Brazil, conservative renovation; and, in Argentina, interrupted rupture. Some conclusions about the impact of educational reform in the selected countries are recuperated through the analysis of 186 academic texts on the subject.

Políticas sociais de atendimento às crianças e aos adolescentes no Brasil

This article analyses the emergence and development of social policies for children and adolescents attendance that are in line with the development process of the Brazilian social protection system, focusing on some of the main representations attributed to childhood, according to the historical and political periods. It seeks to present the notion of childhood instituted under the constitution of the Brazilian welfare state, in such a way as to place it within the broader context of the historical and political transformations that involved the emergence and consolidation of the social policies directed towards children and adolescents in Brazil in the 20th century and the beginning of the 21st.

Reflexão sobre alguns desafios do ensino médio no Brasil hoje

The purpose of this article is to develop some ideas that may contribute to the debate about the secondary education in Brazil, giving emphasis to the conditions existing in the educational institutions, the ongoing educational policies and the challenges posed by the social, economic and political reality of the country. It is also discussed the political, social and economic importance of the expansion and the compulsory character of secondary education as well as the school's culture dimension, in its relationship to the so-called knowledge society. Finally, the role of secondary education for the youth and the new demands it poses for teachers are presented, among some others aspects.

Governamentalidade democrática e ensino de Filosofia no Brasil contemporâneo

This article takes the concepts of biopower and governmentality as the starting point for an analysis of certain recent Brazilian government documents about the introduction of Philosophy as a subject in secondary school. In the 1980s, this argument centered on Philosophy's so-called criticism and its potential for preparing citizens for a democratic society, was used by the movements aimed to restore democracy in Brazil. This argument appears to have been assimilated by the Brazilian government, because it is stated in the Guidelines and Bases of Education Law, secondary school students should demonstrate knowledge of philosophy necessary for the exercise of citizenship. The argument also appears in documents such as the PCN and PCN+ (National Curricular Parameters) and OCEM (Curriculum Guidelines for Secondary School) in their chapters on Philosophy. These documents are examined here in the light of governmentality, making explicit how Philosophy is equipped to train young people according to what is understood as a modern democratic society.

Organização de pesquisa no Brasil: lições do passado, propostas para o futuro

Chemical research in Brazil has grown significantly in the past 20 years, largely thanks to the Brazilian S&T Development Program of the federal government (PADCT). However, the newly achieved levels of highly qualified manpower and research infra-structure require new research organization frameworks to make science, technology and innovation really useful and meaningful for the citizens. The current requirements for creating viable networks of academic and industry researchers are presented and discussed as well as some structural and procedural bottlenecks that have to be eliminated, to achieve maximum high-quality science, technology and relevant innovation output.

25 anos de quimiometria no Brasil

Chemometric activities in Brazil are described according to three phases: before the existence of microcomputers in the 1970s, through the initial stages of microcomputer use in the 1980s and during the years of extensive microcomputer applications of the ´90s and into this century. Pioneering activities in both the university and industry are emphasized. Active research areas in chemometrics are cited including experimental design, pattern recognition and classification, curve resolution for complex systems and multivariate calibration. New trends in chemometrics, especially higher order methods for treating data, are emphasized.