965 resultados para ELISA Kits
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Description based on vol. 1.
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"May 1982"--Vol. 1.
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Includes index.
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Includes index.
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Includes index.
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"10 June 1973."
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"Marine Corps stock list SL-4-07536A."
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Includes index.
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"Mounting kit M241E5 1450-00-078-1217 accessory kit M219E1 1450-00-179-5317 accessory kit M218E1 1450-00-179-5318 mounting kit M184 1450-00-179-6095 Pershing 1a Field Artillery Missile System.
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Mode of access: Internet.
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Thesis (Master's)--University of Washington, 2016-06
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The involvement of oxidatively modified low density lipoprotein (LDL) in the development of CHD is widely described. We have produced two antibodies, recognizing the lipid oxidation product malondialdehyde (MDA) on whole LDL or ApoB-100. The antibodies were utilized in the development of an ELISA for quantitation of MDA-LDL in human plasma. Intra- and inter-assay coefficients of variation (% CV) were measured as 4.8 and 7.7%, respectively, and sensitivity of the assay as 0.04 μg/ml MDA-LDL. Recovery of standard MDA-LDL from native LDL was 102%, indicating the ELISA to be specific with no interference from other biomolecules. Further validation of the ELISA was carried out against two established methods for measurement of lipid peroxidation products, MDA by HPLC and F2-isoprostanes by GC-MS. Results indicated that MDA-LDL is formed at a later stage of oxidation than either MDA or F2- isoprostanes. In vivo analysis demonstrated that the ELISA was able to determine steady-state concentrations of plasma MDA-LDL (an end marker of lipid peroxidation). A reference range of 34.3 ± 8.8 μg/ml MDA-LDL was established for healthy individuals. Further, the ELISA was used to show significantly increased plasma MDA-LDL levels in subjects with confirmed ischemic heart disease, and could therefore possibly be of benefit as a diagnostic tool for assessing CHD risk. © 2003 Elsevier Inc.