The ins and outs of transcriptional control: nucleocytoplasmic shuttling in development and disease

Recent findings relating to SOX transcription factors indicate that defects in organogenesis can be caused not only by impairment of the biochemical properties of transcription factors but also, in some cases, by deficient nuclear import. In addition, experimentally interfering with the nuclear export signals of some SOX factors has now been found to cause developmental defects. Controlling the balance of nuclear import and export might be a common means by which transcription factor activity can be regulated during development, and defects in these processes might underlie a broader spectrum of inherited developmental disorders.

Apoptotic cell removal in development and tissue homeostasis

Cell deletion is a physiological process for the development and maintenance of tissue homeostasis in metazoa. This is mainly achieved by the induction of various forms of programmed cell death followed by the recognition and removal of the targeted cells by phagocytes. In this review, we will discuss cell deletion in relation to the development and function of the innate immune system, particularly of the mononuclear phagocyte system (MPS), its ontogeny and potential role in tissue remodeling in the embryo and adult. Ongoing studies are addressing the roles of professional phagocytes of the MPS and neighboring tissue cells in dying cell removal, and candidate molecules that might attract mononuclear phagocytes to the dying cells. The potential phagocyte must discriminate between living and dying cells; current concepts for this discrimination derive from the observation of newly exposed ligands on the dying cells and new evidence for direct inhibition of uptake by viable cells.

Employer perceptions of skills gaps in retail:issues and implications for UK retailers

Purpose – This paper seeks to identify the skills gaps associated with retail employees in SME and multiple retail companies, and to investigate the potential training and business implications that arise from these skills gaps, from the point of view of retail employers. Design/methodology/approach – Research was conducted within one geographical region and across five counties within the UK. Telephone and face-to-face interviews and focus group workshops were conducted, resulting in responses from 52 retailers. Findings – The key issues and areas of concern to emerge were: the industry image and impact on recruitment and retention; employee and management skills gaps; and barriers to training. Research limitations/implications – The findings highlight the need for UK retail industry to raise the image of the sector, to identify the skills sets for specific roles, and to clarify the retail qualifications and training required delivering these. Originality/value – Succeeds in identifying the skills gaps associated with retail employees in SME and multiple retail companies and in investigating the potential training and business implications arising from these skills gaps.

The potential role of environmental exposures and genomic signaling in development of central nervous system tumors

The etiology of central nervous system tumors (CNSTs) is mainly unknown. Aside from extremely rare genetic conditions, such as neurofibromatosis and tuberous sclerosis, the only unequivocally identified risk factor is exposure to ionizing radiation, and this explains only a very small fraction of cases. Using meta-analysis, gene networking and bioinformatics methods, this dissertation explored the hypothesis that environmental exposures produce genetic and epigenetic alterations that may be involved in the etiology of CNSTs. A meta-analysis of epidemiological studies of pesticides and pediatric brain tumors revealed a significantly increased risk of brain tumors among children whose mothers had farm-related exposures during pregnancy. A dose response was recognized when this risk estimate was compared to those for risk of brain tumors from maternal exposure to non-agricultural pesticides during pregnancy, and risk of brain tumors among children exposed to agricultural activities. Through meta-analysis of several microarray studies which compared normal tissue to astrocytomas, we were able to identify a list of 554 genes which were differentially expressed in the majority of astrocytomas. Many of these genes have in fact been implicated in development of astrocytoma, including EGFR, HIF-1α, c-Myc, WNT5A, and IDH3A. Reverse engineering of these 554 genes using Bayesian network analysis produced a gene network for each grade of astrocytoma (Grade I-IV), and ‘key genes’ within each grade were identified. Genes found to be most influential to development of the highest grade of astrocytoma, Glioblastoma multiforme (GBM) were: COL4A1, EGFR, BTF3, MPP2, RAB31, CDK4, CD99, ANXA2, TOP2A, and SERBP1. Lastly, bioinformatics analysis of environmental databases and curated published results on GBM was able to identify numerous potential pathways and geneenvironment interactions that may play key roles in astrocytoma development. Findings from this research have strong potential to advance our understanding of the etiology and susceptibility to CNSTs. Validation of our ‘key genes’ and pathways could potentially lead to useful tools for early detection and novel therapeutic options for these tumors.

The Effect of Rapport Building in Police Interrogations: Can Rapport Improve the Diagnosticity of Confession Evidence?

Police investigators rely heavily on eliciting confessions from suspects to solve crimes and prosecute offenders. Therefore, it is essential to develop evidence-based interrogation techniques that will motivate guilty suspects to confess but minimize false confessions from the innocent. Currently, there is little scientific support for specific interrogation techniques that may increase true confessions and decrease false confessions. Rapport building is a promising possibility. Despite its recommendation in police interrogation guidelines, there is no scientific evidence showing the effect of rapport building in police interrogations. The current study examined, experimentally, whether using rapport as an interrogation technique would influence participants’ decisions to confess to a wrongdoing. It was hypothesized that building rapport with participants would lead to more true confessions and fewer false confessions than not building rapport. One hundred and sixty nine undergraduates participated in the study. Participants worked on logic problems together and individually, with a study confederate. The confederate asked half of the participants for help in one of the individual problems – effectively breaking the rules of the study. After working on these problems, a research assistant playing the role of interviewer came into the room, built rapport or not with participants, accused all participants of cheating by sharing answers on the individual problems, and asked them to sign a statement admitting their guilt. Results indicated that guilty participants were more likely to sign the confession statement than innocent participants. However, there were no significant differences on participants’ confession decisions based on the level of rapport they experienced. Results do not provide support for the hypothesis that building rapport increases the likelihood of obtaining true confessions and decreases the likelihood of obtaining false confessions. These findings suggest that, despite the overwhelming recommendation for the use of rapport with suspects, its actual implementation may not have a direct impact on the outcome of interrogations.

Complementary gradients in expression and binding of ELF-1 and Mek4 in development of the topographic retinotectal projection map

Peer reviewed

Complementary gradients in expression and binding of ELF-1 and Mek4 in development of the topographic retinotectal projection map

Peer reviewed

Gays demonstrate at FBI building in Foley Square

General note: Title and date provided by Bettye Lane.