Surface mucous as a source of genomic DNA from Atlantic billfishes (Istiophoridae) and swordfish (Xiphiidae)

Procedures for sampling genomic DNA from live billfishes involve manual restraint and tissue excision that can be difficult to carry out and may produce stresses that affect fish survival. We examined the collection of surface mucous as a less invasive alternative method for sourcing genomic DNA by comparing it to autologous muscle tissue samples from Atlantic blue marlin (Makaira nigricans), white marlin (Tetrapturus albidus), sailfish (Istiophorus platypterus), and swordfish (Xiphias gladius). Purified DNA from mucous was comparable to muscle and was suitable for conventional polymerase chain reaction, random amplified polymorphic DNA analysis, and mitochondrial and nuclear locus sequencing. The nondestructive and less invasive characteristics of surface mucous collection may promote increased survival of released specimens and may be advantageous for other marine fish genetic studies, particularly those involving large live specimens destined for release.

A inteligência cristalizada em diferentes contextos socioculturais

Um primeiro objetivo desse estudo foi apresentar uma revisão sobre o que a literatura científica atual fala acerca da bateria de inteligência WISC-III. Os referenciais teóricos que embasam a construção dos testes de inteligência, tais como o modelo de Cattell- Horn Caroll e abordagens mais recentes foram explicitados. A partir das concepções de inteligência fluída (Gf) e cristalizada (Gc), buscou-se um dialógo com pressupostos básicos da psicologia evolucionista do desenvolvimento, considerando os planos de análise ontogenético e filogenético. Foram apresentados aspectos culturais que influenciam desenvolvimento do nosso percurso cognitivo. Teoricamente discutiu-se sobre as causas culturais que embasam o efeito Flynn, efeito de defasagem de escalas, buscando evidencias se nos subtestes da Escala Verbal da bateria WISC-III, adaptada ao contexto brasileiro, existiu sua incidência. Um segundo objetivo deste estudo foi identificar se haveria correlação entre variáveis tais como o uso de tecnologias, o acesso a mídias pelas crianças, práticas de criação e nos resultados obtidos em subtestes da escala WISC-III em crianças de 6 a 11 anos que residem no Rio de Janeiro. Participaram do estudo 25 crianças e seus respectivos responsáveis. Os resultados indicaram que aspectos do cuidado parental e maior acesso a recursos tecnológicos correlacionaram-se com os índices de QI na escala verbal da bateria WISC-III, sugerindo uma indissociável relação entre cultura e inteligência. Acredita-se que tais exposições podem contribuir para ampliar a compreensão da interpretação dos escores fornecidos pela escala WISC-III adaptada ao contexto brasileiro.

The U.S. Fish Commission Steamer Albatross: A History (Papers from a Symposium)

At her launch on 19 October 1882 in Wilmington, Del., the Albatross was the world’s first large deep-sea oceanographic and fisheries research vessel, and she would go on to have a distinguished 40-year career, ranging from the north Atlantic Ocean to the Gulf of Mexico, around Cape Horn in 1887–88, and into the North Pacific. By 1908, Deputy Fish Commissioner Hugh M. Smith reported that “The Albatross has contributed more to the knowledge of marine biology than has any other vessel.” And, of course, her career continued for another 13 years, being decommissioned in late 1921, serving later as a training vessel for nautical cadets, and disappearing from the records in Hamburg, Germany, in late 1928.

Projetos de trabalho na Creche UFF: articulação com a proposta pedagógica e a produção das crianças

A presente dissertação traz os resultados da pesquisa que teve como objetivo investigar a articulação entre os projetos de trabalho, a proposta pedagógica e a produção das crianças na Creche UFF, no espaço físico do Departamento de Educação Infantil do Colégio Universitário Geraldo Reis (COLUNI/UFF), Unidade Universitária de Educação Infantil (UUEI) localizada em Niterói, Rio de Janeiro (RJ). A investigação tomou como material de análise a produção do conhecimento sobre a infância restrita a projetos de trabalho de autoria de docentes, estagiários, bolsistas, pesquisadores em geral, tendo como campo de pesquisa a Creche UFF; os projetos de trabalho desenvolvidos nessa unidade com as crianças de dezoito meses a cinco anos; os registros do caderno de campo produzidos a partir das observações das reuniões pedagógicas. Para consecução da proposta, foram utilizados estudos sobre projetos realizados por autores tais como Fernando Hernández, Montserrat Ventura, Edwards, Gandini e Forman, Maria Carmem Barbosa e Maria da Graça Souza Horn. Para as orientações curriculares, analisaram-se os estudos de Ligia Aquino e Vera Vasconcellos, bem como os principais documentos oficiais para a educação infantil. A pesquisa teve como resultados a constatação de que há articulação entre os projetos de trabalho e a proposta pedagógica, assim como há visibilidade, nos portfólios, das produções das crianças

Species management in aquatic habitats WRc RD Interim 1997

This is the Species management in aquatic Habitats WRc Interim 1997 document produced by the Environment Agency in 1997. This document reports progress on R&D Project 640, which aims to provide information on species of conservation value of particular relevance to the Environment Agency, in relation to its activities affecting aquatic environments. A range of stand-alone outputs is being produced, comprising Species Action Plans, practical management guidelines for Agency staff and third parties, and various research outputs to improve the knowledge base on the status and ecological requirements of priority species. The species of conservation values are: water shrew, daubenton’s bat, Kingfisher, yellow wagtail, Grey wagtail, sand martin, reed bunting, dipper, marsh warbler, great crested new, spined loach, brook lamprey, river lamprey, sea lamprey, shining rams-horn snail, little whirlpool rams-horn snail, depressed river mussel, a freshwater pea mussel, native crayfish, and triangular club-rush. The process of species selection was altered during the course of the project by the report on biodiversity by the UK Biodiversity Steering Group (1995). Whilst still including species that were not particularly endangered but were greatly influenced by the activities of the Agency, the project addressed species on the ‘short’ and ‘middle’ priority lists of the Biodiversity report, particularly those for which the Agency had specific responsibilities.

Examination of the foraging habits of Pacific harbor seal (Phoca vitulina richardsi) to describe their use of the Umpqua River, Oregon, and their predation on salmonids

The increase in harbor seal (Phoca vitulina richardsi) abundance, concurrent with the decrease in salmonid (Oncorhynchus spp.) and other fish stocks, raises concerns about the potential negative impact of seals on fish populations. Although harbor seals are found in rivers and estuaries, their presence is not necessarily indicative of exclusive or predominant feeding in these systems. We examined the diet of harbor seals in the Umpqua River, Oregon, during 1997 and 1998 to indirectly assess whether or not they were feeding in the river. Fish otoliths and other skeletal structures were recovered from 651 scats and used to identify seal prey. The use of all diagnostic prey structures, rather than just otoliths, increased our estimates of the number of taxa, the minimum number of individuals and percent frequency of occurrence (%FO) of prey consumed. The %FO indicated that the most common prey were pleuronectids, Pacific hake (Merluccius productus), Pacific stag-horn sculpin (Leptocottus armatus), osmerids, and shiner surfperch (Cymatogaster aggregata). The majority (76%) of prey were fish that inhabit marine waters exclusively and fish found in marine and estuarine areas (e.g. anadromous spp.) which would indicate that seals forage predominantly at sea and use the estuary for resting and opportunistic feeding. Salmonid remains were encountered in 39 samples (6%); two samples contained identifiable otoliths, which were determined to be from chi-nook salmon (O. tshawytscha). Because of the complex salmonid composition in the Umpqua River, we used molecular genetic techniques on salmonid bones retrieved from scat to discern species that were rare from those that were abundant. Of the 37 scats with salmonid bones but no otoliths, bones were identified genetically as chinook or coho (O. kisutch) salmon, or steelhead trout (O. mykiss) in 90% of the samples.

Sulphur and methionine content of some fresh water fishes of Madras

The importance of sulphur amino acids, especially of methionine, in our nutrition is too well known to be emphasized. But adequate data on the sulphur and methionine contents of the cheapest of our animal foods viz., fish, are not available. In this note, the total sulphur and methionine content of 18 common fresh water fishes is presented. Total sulphur was determined by Osborne perioxide method (Winton & Winton, 1945) and methionine by the colorimetric method of Horn. (Horn et al., 1946)

On the troll line investigations off Cochin during five fishing seasons. Pt. 2. Investigations during 1963-64 season

During this season the investigations were mainly directed towards elucidation of the selective action of trolling lures. Feather jigs, buffalo horn jigs, stainless steel jigs, Japanese whale bone jigs and plastic jigs were selected. Operations were carried out from Fisheries technology No. 5.

Standardisation of specification for different trolling lures

Fishing experiments were conducted with buffalo horn, wooden and galvanised iron jigs of different lengths and weights rigged in troll lines. It was observed that jigs with 12 cm length and 60 g weight were superior to others in luring seer fish. Fish head jigs of 60 g weight showed a higher efficiency index than those of 50 and 70 g tried in these studies.

Effects of toxins induced by phytoplankton bloom on physiological function of indicator animals in Anzali Lagoon

The present overview summarizes data from one year's study during the period of 1379 to 1380 in the regions by "Anzali" lagoon called "Abkenar" and "Hendkhale". Specimens from this lagoon obtained weekly during mordad and shahrivar mounths (July 21 to september 21). The study included 67 types of 5 phytoplanktonal phylum. In "Abkenar" region Cyanobacters with maximum of 97% and minimum of 64.5% of total combination of phytoplanktones made the dominating combination during the period of study , while in "hendkhale" chyrsophyta with maximum of 89% and minimum of 38.7% of total phytoplanktonal was the dominating figure at the same period of time. Researches on ecological parameteres showed that the avarage dissolved oxygen in -Abkenar" and "hendlchale" regions was 10.7 and 8.0 mg/lit respectivly, also total rate of Phosphat in these regions was 0.085 and 0.15 mg/lit respectivly. This study showed that the rates of Nitrat and Amonium in "Abkenar" region was 0.043 and 0.79 mg / lit while for the same substances in "Ilendkhale" measured 0.08 and 0.7 mg I lit respective. Also the avarage rate of chlorophyll a in these two areas measured 58,38 and 40.45 j.un /ht respectivly. Depending on results of correlation cofficient in "Abkenar" region we had Cyanobacters , water and air temperature , Chlorophyll a and total amount of Phosphat as a poitive correlation with transparency while Amonium and Nitrat showed , a negative correlation , EC and finally dissolved oxygen showed a very low rate of correlation coffiocient. To perform this research 5 genus of Cyanobacteres horn "Anzali" lagoon have been isolated and cultured in a laboratorial conditions Later by using Mouse Bioassay method one of these genus identified as a toxic algae. Levels of LD50 with intra peritoneal injection of toxin on mouse in 24 hours was 660 mg/kg and Levels of LC50 by using Bioassay method on Artemia and Daphnia has been shown 618 and 1000 mg kg respectively. Also the physiological effects of were investigated. algae on two types of Cypronides family called Cyprinus carpi° and Hypophrthabnichthys Resultes of blood analyses of Cypronides who were feeded by toxic algae showed a significant decline (P < 0.05) in white and red blood cells and their hematocrites. Levels of LDH , SGOT and SGPT in their blood serum had a significant increase in porportion to control group (P < 0.05) but there was no evidance a differences in Total Protein levels. Pathological studies show damage and destruction of hepato pancreas and kidney of these fishes. Signs and symptoms of intoxication caused by Cyanobacter called Planktohrix agardlltii in mouse and fish show heptotoxic character. This toxin belongs to cyclicpeptides of microcystines group.

Circulating immunoglobulin A- and immunoglobulin G-secreting hybridoma cells in peripheral blood preferably migrate to female genital tracts. The role of sex hormones

Antigen-specific circulating immunoglobulin-secreting cells (ISC) migrate to various secondary and tertiary lymphoid tissues. To understand the migration of the cells into the genital tract and its regulation by sex hormones, spleen-derived SG2 hybridoma cells secreting immunoglobulin G2b (IgG2b) and Peyer's patch-derived PA4 hybridoma cells secreting polymer IgA were labelled with (3) H-TdR, and intravenously injected into syngeneic mice of both sexes. Using flow cytometry, surface molecular markers of plasma cells, CD38 and CD138, and adhesion molecules, CD49d, CD162, and CD11a were found to be positive in SG2 and PA4 cells, but CD62L, alpha4beta7 and CD44 were not expressed on these cells. The relative distribution indexes (RDIs) of the cells in genital tract and other tissues were measured. The means of RDIs of SG2 and PA4 cells in female genital tissues were 6.5 and 4.5 times as many as the means in male genital tissues, respectively. The treatment of ovariectomized mice with beta-oestradiol significantly increased the RDIs of PA4 cells in cervix and vagina, but decreased the RDIs of SG2 cells in vagina, horn of uterus, uterus and rectum (P <0.05). Progesterone treatment increased the RDIs of PA4 cells in vagina and rectum (P <0.05). The treatment with testosterone significantly increased the RDIs of SG2 and PA4 cells in epididymis and accessory sex glands (P <0.05). These results demonstrate that the female genital tract is the preferable site for the migration of circulating hybridoma cells to the male genital tract, and sex hormones play an important role in regulation of the migration of circulating ISC to genital tracts.

Facets of prior experience and the effectiveness of inclusive design

Research in inclusive design has shown the importance of prior experience for the usability of interactive products. Prior experience, however, is an ill-defined and inconsistently used construct. A number of different definitions and operationalisations of experience exist, but the differing power of these operationalisations to predict the usability of products for older users has rarely been investigated systematically. This study seeks to fill that gap. It is argued that the construct of experience has at least three components. It is proposed that two of these components, exposure and competence, are directly relevant for the current discussion about prior experience in inclusive design and that they can predict to different degrees the usability of a product for older users. In an empirical study, these facets of expertise are each operationalised on three levels of specificity and their impact on usability is assessed. The results show that measures of competence predict usability variables more strongly than measures of exposure and that levels of medium and high specificity are the best predictors. The application of inclusive design principles to a redesigned version of a ticket vending machine-although not resulting in a difference of overall usability-changed the impact of prior experience on usability measures implying an enhanced inclusiveness of the redesign with regard to prior experience. The implications of these findings for the effectiveness of inclusive design for older users are discussed. © 2013 Springer-Verlag Berlin Heidelberg.

Morphology, biometry and distribution of Difflugia biwae Kawamura, 1918 (Protozoa : Rhizopoda)

The freshwater testate amoeba Difflugia biwae Kawamura, 1918, isolated from Mulan Lake, Hubei Province, China is investigated using light microscopy and scanning electron microscopy. The morphology, biometry and distribution of this little known species are supplied. After careful comparison with three other similar species, including D. delicatula Gauthier-Livre et Thomas, D. elegans Penard and D. oblonga caudata Stepanek, we believe that the characteristics of smooth fusiform shell, conspicuous great collar flare (always larger than body-width) around the aperture, constriction behind the aperture and a somewhat curved aboral horn set D. biwae apart from all other Difflugia species. This species shows a great diversity in total length, collar height and aboral horn length which have high variability (CV between 11.76 and 24.52). However, body width, collar diameter, neck width, body length and aperture diameter are fairly constant with low variability (CV between 5.34 and 8.79) which shows a remarkable uniformity of D. biwae. Also, the size frequency distributions of both body width and body length yield bell-shaped (normally distributed) curves and indicate that D. biwae is a size-monomorphic species, characterized by a main-size class and a small size range. D. biwae is probably endemic to East Asia (China and Japan) because it has such a large size (165-306 mu m) that it would have been easily found in Europe and North America, if it were there. Consequently, D. biwae must have a restricted geographical distribution, disproving the old hypothesis that microscopic organisms are cosmopolitan.

语义Web规则标记语言OWLRule+的设计与实现

语义Web是对未来Web体系结构的一个伟大设想 ,其研究分层次进行 目前足够成熟的最高层是以OWL语言为代表的ontology层 但它的语义仅限于描述逻辑 ,该逻辑主要表示对象和类的层次结构 ,而规则的表达能力弱 因此在其之上需要一种表达力更丰富的逻辑语言 设计了一种新的语义Web规则标记语言OWLRule+:其语法扩展了OWL ;语义基于CARIN ,一种结合描述逻辑和Horn规则的表示语言 ;实现基于Jess规则推理机 实例学习展示了它对目前的Webontology语言在规则表示和推理能力上的扩展