Formation and Turnover of NSF- and SNAP-containing “Fusion” Complexes Occur on Undocked, Clathrin-coated Vesicle–derived Membranes

Specificity of vesicular transport is determined by pair-wise interaction between receptors (SNAP receptors or SNAREs) associated with a transport vesicle and its target membrane. Two additional factors, N-ethylmaleimide-sensitive fusion protein (NSF) and soluble NSF attachment protein (SNAP) are ubiquitous components of vesicular transport pathways. However, the precise role they play is not known. On the basis that NSF and SNAP can be recruited to preformed SNARE complexes, it has been proposed that NSF- and SNAP-containing complexes are formed after SNARE-dependent docking of transport vesicles. This would enable ATPase-dependent complex disassembly to be coupled directly to membrane fusion. Alternatively, binding and release of NSF/SNAP may occur before vesicle docking, and perhaps be involved in the activation of SNAREs. To gain more information about the point at which so-called 20S complexes form during the transport vesicle cycle, we have examined NSF/SNAP/SNARE complex turnover on clathrin-coated vesicle–derived membranes in situ. This has been achieved under conditions in which the extent of membrane docking can be precisely monitored. We demonstrate by UV-dependent cross-linking experiments, coupled to laser light-scattering analysis of membranes, that complexes containing NSF, SNAP, and SNAREs will form and dissociate on the surface of undocked transport vesicles.

Mammalian ubiquitin-conjugating enzyme Ubc9 interacts with Rad51 recombination protein and localizes in synaptonemal complexes.

Hsubc9, a human gene encoding a ubiquitin-conjugating enzyme, has been cloned. The 18-kDa HsUbc9 protein is homologous to the ubiquitin-conjugating enzymes Hus5 of Schizosaccharomyces pombe and Ubc9 of Saccharomyces cerevisiae. The Hsubc9 gene complements a ubc9 mutation of S. cerevisiae. It has been mapped to chromosome 16p13.3 and is expressed in many human tissues, with the highest levels in testis and thymus. According to the Ga14 two-hybrid system analysis, HsUbc9 protein interacts with human recombination protein Rad51. A mouse homolog, Mmubc9, encodes an amino acid sequence that is identical to the human protein. In mouse spermatocytes, MmUbc9 protein, like Rad51 protein, localizes in synaptonemal complexes, which suggests that Ubc9 protein plays a regulatory role in meiosis.

A classified catalogue of theological and religious books, comprising a large collection of standard works, American and foreign; old and rare works, and recent publications, to September 1, 1871. Also an addenda of miscellaneous literature, works of reference, &c. For sale by Smith, English & Co., booksellers and importers ...

Interleaved.

Instrumentation for measuring pavement-vehicle interaction. Volume III: Kennedy Co. operation and maintenance manual, formatter and digital tape transport. Final report.

Federal Highway Administration, Structures and Applied Mechanics Division, Washington, D.C.

Report to the President by the Emergency Board created June 23, 1948 by Executive order 9971 pursuant to Section 10 of the Railway Labor Act : to investigate and report upon a dispute between the Grand Trunk Western Railroad Co., Chesapeake and Ohio Railway Co., Wabash Railroad and the Ann Arbor Railroad Co. and certain of its employees represented by the National Maritime Union of America, N.M.B. cases A-2801, A-2802, A-2803, A-2804, Detroit, Michigan, July 20, 1948.

Signed: Robert G. Simmons, chairman, Thomas F. Gallagher, member, Joseph L. Miller, member.

Report to the President by the Emergency Board appointed March 15, 1949 by Executive order 10045 pursuant to Section 10 of the Railway Labor Act, as amended : to investigate and report in respect to a dispute involving the Wabash Railroad Co. and the Ann Arbor Railroad Co., carriers, and certain of their employees, represented by the Brotherhood of Locomotive Engineers, Brotherhood of Locomotive Firemen and Enginemen, Order of Railway Conductors, and Brotherhood of Railroad Trainmen : (NMB case A-3028) : St. Louis, Mo., April 6, 1949.

Signed: Roger I. McDonough, chairman, Curtis G. Shake, member, John W. Yeager, member.

Federal Council bulletin. A journal of religious co-operation and inter-church activities.

Caption title; no index.

Discourses on the Divine Unity or, A Scriptural proof and demonstration of the one supreme deity, of the God and Father of all ; and of the subordinate character and inferior nature of our Lord Jesus Christ: with a confutation of the doctrine of a co-equal and consubstantial Trinity in unity ; and a full reply to the objections of Trinitarians /

Mode of access: Internet.

A printed specimen of Caslon old style type, with appropriate ornaments, being the first of a series of books showing the many beautiful types in the composing-room of Redfield-Kendrick-Odell co., printers and map makers.

"Of this book of Caslon old style type, there have been printed five hundred copies, of which this is number 272"

Study guide to accompany Principles of sociology (rev. ed., Century co. 1930) and Test and challenges in sociology (Century co. 1931) by Edward Alsworth Ross,

Mode of access: Internet.