What Baptists believe, and other discourses.

With this is bound, as issued, the author's The curse and the cross ... Baltimore, 1887.

1001 questions and answers on United States history; including the Constitution and amendments.

Mode of access: Internet.

The discoveries in Crete and their bearing on the history of ancient civilisation,

Bibliography: p. 231-236.

The register of the visitors of the University of Oxford, from A.D. 1647 to A.D. 1658.

Mode of access: Internet.

The Annual of the American Schools of Oriental Research.

Editors: 1919/20, C. C. Torrey; 1921/22, W. J. Moulton; 1922/23-1924/25, B. W. Baxon; 1925/26-1930/31, H. J. Cadbury; 1931/32- M. Burrows and E. A. Speiser.

Plan of water pipes. Grounds of J.J. Reimers.

Ink on tracing paper; residence by Temple and Burrows; signed; 77 x 67 cm.; Scale: 1" = 20' [from photographic copy by Lance Burgharrdt]

Planting plan. Country home of J.J. Reimers.

Ink on linen; location, type of plantings; gardens, arbors, steps, urns; residence by Temple and Burrows; signed; 82 x 73 cm.; Scale: 1" = 20' [from photographic copy by Lance Burgharrdt]

Plan of garden. Grounds of Mr. J.J. Reimers.

Red, black ink on tracing paper; plan, sections; elevations, distances; residence by Temple and Burrows; signed; 86 x 43 cm.; Scale: 1" = 10' [from photographic copy by Lance Burgharrdt]

School of Nursing Class of 2000

Top Row: Michelle Arsulowicz, Christy L. baginski, Sarah Ball, Jessica Beitner, Gina Blank, Kelley Brown, Cheryl Buckler, Loredana Bugan, Kelly Burrows, Cynthia Carillo, Raquel Casarez, Rachel Cieslak, Nicole Clark, Stephanie Cochran, John condon

Row 2: Becky Cooper, Claire Coughlan, Heidi M. Dillenbeck, Jennfier Long, Tracy Adame, Stacy B. Buchanan, Tiffany A. Fellberg, Elise H. Peterson, Jessica Na, Abigail Sikkenga, Michelle, Falatko, Lisa Dolan, Laura Dorman, Suzanne Ewald

Row 3: Heather Fix, Anna galczyk, Inessa Gankin, Elizabeth Gardner, Jennfier Gray, Katy Gudritz, Lindsey Hancock, Christina Haremski, Marcie Harless, Anne Hartgerink

Row 4: Benjamin Hatchett, Karrie Herdus, Lacy Hillman, Amy Hlavka, Nicole Holt, Julie Huss, Jessica Inwood, Sujuan Johnson, Nicole N. Jones, Jennfier M. kenny

Row 5: Lori Khami, Jennifer Kiebler, Nipa Kinariwala, Kelli Kingma, Elizabeth Kubis, Jillian LaPrairie, Heather Livermore, Emily Long-Minard, Miki Loveland, Meyhan Manion, David Markiewicz, Dareth McCoy

Row 6: Kerri McElmeel, Suzanne McQuaid, Katie Murphy, Marie Murray, Nola Pender, Carol Loveland-Cherry, Ada Sue Hinshaw, Susan Boehm, Beverly Jones, Patricia coleman-Burns, Darlene Najor, Kelly Nowak, Charisse Patterson, Jennfier Pliska

Row 7: Heather Prusi, Erika Punches, Gena ramazetti, Kimberly Rendz, Suzanne Robben, Sara Robbins, Lori rottschafer, Annette Sandretto, Sara Schad, Chad Schenavar, Jennifer Seamon, Katherine Shell, Charlotte L. Sims, Caryn Steenland, Andrea Stutzman

Row 8: Jennifer Thibault, stacey Turnipseed, Linda Twomey, Stephanie Van Eyk, Maryanne VanNasdale, Annemarie Vassalo, Anna Walawender, Mark Warren, Nicole Weber, Kindra Weid, Lela Shitley, Kristy Wierzba, Shari Wilkinson, Lily Wu

Effect of aestivation on long bone mechanical properties in the green-striped burrowing frog, Cyclorana alboguttata

The green-striped burrowing frog, Cyclorana alboguttata, survives extended drought periods by burrowing underground and aestivating. These frogs remain immobile within cocoons of shed skin and mucus during aestivation and emerge from their burrows upon heavy rains to feed and reproduce. Extended periods of immobilisation in mammals typically result in bone remodelling and a decrease in bone strength. We examined the effect of aestivation and, hence, prolonged immobilisation on cross-sectional area, histology and bending strength in the femur and tibiolibula of C alboguttata. Frogs were aestivated in soil for three and nine months and were compared with control animals that remained active, were fed and had a continual supply of water. Compared with the controls, long bone size, anatomy and bending strength remained unchanged, indicating an absence of disuse osteoporosis. This preservation of bone tissue properties enables C. alboguttata to compress the active portions of their life history into unpredictable windows of opportunity, whenever heavy rains occur.

Selection pressure-driven evolution of the Epstein-Barr virus-encoded oncogene LMP1 in virus isolates from southeast Asia

The geographically constrained distribution of Epstein-Barr virus (EBV)-associated nasopharyngeal carcinoma (NPC) in southeast Asian populations suggests that both viral and host genetics may influence disease risk. Although susceptibility loci have been mapped within the human genome, the role of viral genetics in the focal distribution of NPC remains an enigma. Here we report a molecular phylogenetic analysis of an NPC-associated viral oncogene, LMP1, in a large panel of EBV isolates from southeast Asia and from Papua New Guinea, Africa, and Australia, regions of the world where NPC is and is not endemic, respectively. This analysis revealed that LMP1 sequences show a distinct geographic structure, indicating that the southeast Asian isolates have evolved as a lineage distinct from those of Papua New Guinea, African, and Australian isolates. Furthermore, a likelihood ratio test revealed that the C termini of the LMP1 sequences of the southeast Asian lineage are under significant positive selection pressure, particularly at some sites within the C-terminal activator regions. We also present evidence that although the N terminus and transmembrane region of LMP1 have undergone recombination, the C-terminal region of the gene has evolved without any history of recombination. Based on these observations, we speculate that selection pressure may be driving the LMP1 sequences in virus isolates from southeast Asia towards a more malignant phenotype, thereby influencing the endemic distribution of NPC in this region.

Endogenous Presentation of CD8+ T Cell Epitopes from Epstein-Barr Virus–encoded Nuclear Antigen 1

Epstein-Barr virus (EBV)-encoded nuclear antigen (EBNA)1 is thought to escape cytotoxic T lymphocyte (CTL) recognition through either self-inhibition of synthesis or by blockade of proteasomal degradation by the glycine-alanine repeat (GAr) domain. Here we show that EBNA1 has a remarkably varied cell type-dependent stability. However, these different degradation rates do not correspond to the level of major histocompatibility complex class I-restricted presentation of EBNA1 epitopes. In spite of the highly stable expression of EBNA1 in B cells, CTL epitopes derived from this protein are efficiently processed and presented to CD8(+) T cells. Furthermore, we show that EBV-infected B cells can readily activate EBNA1-specific memory T cell responses from healthy virus carriers. Functional assays revealed that processing of these EBNA1 epitopes is proteasome and transporter associated with antigen processing dependent. We also show that the endogenous presentation of these epitopes is dependent on the newly synthesized protein rather than the long-lived stable EBNA1. Based on these observations, we propose that defective ribosomal products, not the full-length antigen, are the primary source of endogenously processed CD8(+) T cell epitopes front EBNA1.

Potent T cell response to a class I-binding 13-mer viral epitope and the influence of HLA micropolymorphism in controlling epitope length

The BZLF1 antigen of Epstein-Barr virus includes three overlapping sequences of different lengths that conform to the binding motif of human leukocyte antigen (HLA) B*3501. These 9-mer ((56)LPOGQLTAy(64)), 11-mer ((54)EPLPQGQLTAy(64)), and 13-mer ((52)LPEPLPQGQLTAY(64)) peptides all bound well to B*3501; however, the CTL response in individuals expressing this HILA allele was directed strongly and exclusively towards the 11-mer peptide. In contrast, EBV-exposed donors expressing HLA B*3503 showed no significant CTL response to these peptides because the single amino acid difference between B*3501 and B*3503 within the F pocket inhibited HLA binding by these peptides. The extraordinarily long 13-mer peptide was the target for the CTL response in individuals expressing B*3508, which differs from B*3501 at a single position within the D pocket (B*3501, 156 Leucine; B*3508, 156 Arginine). This minor difference was shown to enhance binding of the 13-mer peptide, presumably through a stabilizing interaction between the negatively charged glutamate at position 3 of the peptide and the positively charged arginine at HLA position 156. The 13-mer epitope defined in this study represents the longest class I-binding viral epitope identified to date as a minimal determinant. Furthermore, the potency of the response indicates that peptides of this length do not present a major structural barrier to CTL recognition.

Morphological and physiological specialization for digging in amphisbaenians, an ancient lineage of fossorial vertebrates

Amphisbaenians are legless reptiles that differ significantly from other vertebrate lineages. Most species dig underground galleries of similar diameter to that of the animal. We studied the muscle physiology and morphological attributes of digging effort in the Brazilian amphisbaenid Leposternon microcephalum (Squamata; Amphisbaenia), which burrows by compressing soil against the upper wall of the tunnel by means of upward strokes of the head. The individuals tested (