In vitro heat exposure induces a redistribution of nuclear matrix proteins in human K562 erythroleukemia cells.

By using both conventional and confocal laser scanning microscopy with three monoclonal antibodies recognizing nuclear matrix proteins we have investigated by means of indirect fluorescence whether an incubation of isolated nuclei at the physiological temperature of 37 degrees C induces a redistribution of nuclear components in human K562 erythroleukemia cells. Upon incubation of isolated nuclei for 45 min at 37 degrees C, we have found that two of the antibodies, directed against proteins of the inner matrix network (M(r) 125 and 160 kDa), gave a fluorescent pattern different from that observed in permeabilized cells. By contrast, the fluorescent pattern did not change if nuclei were kept at 0 degrees C. The difference was more marked in case of the 160-kDa polypeptide. The fluorescent pattern detected by the third antibody, which recognizes the 180-kDa nucleolar isoform of DNA topoisomerase II, was unaffected by heat exposure of isolated nuclei. When isolated nuclear matrices prepared from heat-stabilized nuclei were stained by means of the same three antibodies, it was possible to see that the distribution of the 160-kDa matrix protein no longer corresponded to that observable in permeabilized cells, whereas the fluorescent pattern given by the antibody to the 125-kDa polypeptide resembled that detectable in permeabilized cells. The 180-kDa isoform of topoisomerase II was still present in the matrix nucleolar remnants. We conclude that a 37 degrees C incubation of isolated nuclei induces a redistribution of some nuclear matrix antigens and cannot prevent the rearrangement in the spatial organization of one of these antigens that takes place during matrix isolation in human erythroleukemia cells. The practical relevance of these findings is discussed.

Activity and migration of Cory's shearwater

I describe some of the features that characterize the activity and migration of Cory’s shearwater during approximately one year. I also explore the influence of Moon, photoperiod, geographic position and life-history stage on the resulting patterns and the periodicity of the latter. I have principally used time series and regression analysis. Its use here is one of the first applications to the analysis of logger data in seabirds. An intriguing finding of this work is the lunar periodicity that pervades the annual cycle of this species.

Implantació ERP SAP R/3 a l'empresa Quimiwork

Implantació ERP SAP R/3 a una organització del sector químic.

Report of Recommendation to the Iowa Department of Human Services, June 30, 2003

State Agency Audit Report

Report of Recommendations to the Iowa Legislature, June 30, 2003

State Agency Audit Report

Increased endometrial placenta growth factor (PLGF) gene expression in women with successful implantation.

Objective: To analyze the vascularization of the endometrium via hysteroscopy and to assess its correlation with angiogenic factor gene expression and embryo implantation rate.Design: Cross-sectional study.Setting: Public university hospital.Patient(s): Patients undergoing hysteroscopy for supposed infertility.Intervention(s): Endometrial quality assessment according to Sakumoto-Masamoto, performed in the early secretory phase of the cycle. Collection of an endometrial tissue biopsy.Main Outcome Measure(s): RNA extraction, reverse transcription, and determination of gene expression of angiogenesis- and implantation-relevant factors using quantitative polymerase chain reaction. Retrieval of pregnancy information from the medical records.Result(s): Good quantity/quality RNA with infertility history was obtained from 63 participating women. Those with a "good" endometrium and subsequent pregnancy showed increased gene expression for placenta growth factor when compared with patients with a "bad" endometrium and who did not succeed with pregnancy to date. Nonpregnant women with a "good" endometrium presented an intermediate result. No significant differences were observed for several other genes tested, but trends in the same direction were observed.Conclusion(s): This study demonstrates for the first time that endometrial PLGF expression corresponds to the hysteroscopic appearance of the endometrium, and therefore has potential as a clinically relevant prognosticator for infertility treatment success. (Fertil Steril (R) 2011;96:663-8. (C)2011 by American Society for Reproductive Medicine.)

Aggiornamento biogràfic de Joan Pere Fontanella (1575-1649)

Com tants altres personatges de la nostra història, el pare de FrancescFontanella i Garraver –el jurisconsult olotí Joan Pere Fontanella–ha estat objecte durant dècades d’un encadenament de notíciesbiogràfiques on s’han reiterat dades no sempre filtrades científicament.Això ha abocat diverses generacions a acumular imprecisions sobre ell,fins i tot a distorsionar-ne la significació ideològica i política, sovinten clau d’un patriotisme ardorós. Volem contribuir, sense cap ànimd’esmena, a esclarir i difondre algunes informacions sobre les sevesvida i obra i el seu pensament jurídic i polític. I considerem adientfer-ho en un marc que ratione loci convida a ventilar els forts lligamsque l’advocat mantingué amb la ciutat de Girona i ratione materiaeobliga a subratllar el seu vessant protector dels parents i pròxims i,més a l’engròs, la seva vocació per analitzar amb el dret a la mà elcomplexíssim món de les relacions familiars.

De situ orbis ([Reprod.]) / [trad. de Guarino de Vérone]

[Géographie (latin). 1494]