Análise ampla do genoma para detecção de erros de montagem no genoma de referência bovino e para detecção de locos relacionados a características de produção e reprodução da raça GIR

Pós-graduação em Genética e Melhoramento Animal - FCAV

Expressão diferencial de mRNA em células de cultivo infectadas ple herpesvírus bovino 5

Pós-graduação em Medicina Veterinária - FMVZ

Padronização de técnica manual para obtenção de plasma rico em plaquetas de bovino

To evaluate the biochemical profile and protein concentration of whey from milk samples of healthy Murrah primiparous and pluriparous buffaloes, 30 female buffaloes were analyzed during a complete lactation. The animals were divided into three groups: G1 = 10 primiparous buffaloes, G2 = 10 pluriparous buffaloes with 2-3 lactations and G3 = 10 pluriparous buffaloes with > 3 lactations. The lactation period was divided into: early stage (I: 1-3 months of lactation), intermediate stage (T: 4-6 months of lactation) and final stage (F: 7-9 months of lactation). Before milk sampling, physical examination of the mammary gland, strip cup test and California Mastitis Test (CMT) were performed. After mammary quarters asepsis, 20mL of milk were collected monthly from each mammary quarter, during a complete lactation, in sterilized plastic bottles without preservative, in order to perform microbiological isolation, biochemical profile and protein electrophoresis in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and 30mL of milk from each mammary quarter were collect, in sterilized plastic bottles containing preservative bronopol to perform the somatic cell count (SCC). A total of 1,042 milk samples were collected from the experimental groups during lactation, of which 923 samples showed negative reaction to CMT and negative microbiological isolation and were selected to biochemical profile analysis and protein electrophoresis in SDS-PAGE. There were influence of parity order and stage of lactation in biochemical profile and protein concentration of healthy Murrah buffaloes'whey. Primiparous buffaloes (G1) showed higher gamma-glutamyltransferase (GGT: 2,346 U/L), alkaline phosphatase (ALP: 181 U/L), phosphorus (P; 56.6mg/dL), potassium (K; 32.0mg/dL) and alpha-lactalbumin (458mg/dL). Buffaloes with 2-3 lactations (G2) showed higher SCC (70,700 cells/mL) and higher concentrations of total protein (1.55g/dL), albumin (100mg/dL), magnesium (Mg; 8.80mg/dL), chlorides (Cl; 176mg/dL), iron (Fe; 10.7 mu g/dL), sodium (Na; 178mMol/L) and lactoferrin (59.5mg/dL). Bufalloes with > 3 lactations (G3) showed higher concentrations of total calcium (Ca; 41.8mg/dL), ionized calcium (iCa; 2.92mMol/L), immunoglobulin A (IgA; 1.32mg/dL), serum albumin (99.1mg/dL), immunoglobulin G (IgG; 49.7mg/dL) and beta-lactoglobulin (1,068mg/dL). During lactation it was observed increase in SCC, GGT, ALP, total protein, albumin, P, Mg, Cl, Na, lactoferrin, serum albumin, IgG and alpha-lactalbumin, as well as decrease in concentrations of Ca, Fe, iCa, K, IgA and beta-lactoglobulin in buffaloes'whey. The results may be used as reference for buffaloes and to support diagnosis and prognosis of diseases common to lactation periods.

Detecção de genes de entrotoxinas de Staphylococcus aureus e estafilococos coagulase negativos isolados de leite bovino e de tanques de refrigeração comunitários procedentes de pequenas propriedades da região de Bauru-SP

Pós-graduação em Medicina Veterinária - FMVZ

Estudo neuroanatômico do éncefalo bovino por ressonância magnética

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Proposta de um programa de qualidade o leite e controle da mastite em rebanho bovino de vacas holandesas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Transformações da matéria orgânica em latossolo vermelho tratado com esterco bovino

Pós-graduação em Agronomia (Ciência do Solo) - FCAV

Histologia e histomorfometria do retículo de bovino no período pré-natal

The development of the stomach of ruminants requires further studies because it is a complex process. Therefore, was analyzed the histology and histomorphometry of reticulum at each gestation period. Samples of Nelore fetuses (Bos taurus indicus) was divided in 5 groups: 1 - fetuses at 9 to 15 weeks (8 to 21cm) of gestation, 2 - fetuses at 16 to 22 weeks (23 to 37cm) 3 - fetuses at 23 to 29 weeks (40 to 58cm), 4 - fetuses at 30 to 36 weeks (61 to 77cm) and 5 - fetuses at 37-43 weeks (79 to 88cm). The histological sections were stained with hematoxylin and eosin and Mallory's trichrome and examined by light microscopy. In group 1, the fetuses with 11 cm, showed mucosal projections that eventually become primary crests, lamina propria and submucosa are fused and external and internal muscular and similar. At 16.5 inches, the primary crests had lamina propria and at 18.5 cm, appeared connections between the ridges. In group 2, at 31 cm started epithelial involvement around the crests. In group 3, the fetuses of 42 cm revealed the first secondary papillae and muscular mucosa in the upper portion of the primary crests. Thereafter, it was increased epithelial due to its positioning around the crests, thickening of the lamina propriasubmucosa, muscular layer, especially the internal muscular and serosa. It was concluded that the most visible changes occur in fetuses belonging to the first 3 groups, measurements for the epithelium, muscle layer and total wall were increasing and the other analyzed layers showed variations during fetal development.

Detecção de Histophilus somni (Haemophilus somnus) no sêmen bovino mediante reação em cadeia pela polimerase (PCR)

The present study evaluated the use of PCR for Histophilus somni detection in bovine semen. Semen samples were experimentally infected with H. somni at dilutions ranging from 107 to 101 bacteria/mL and subjected to DNA extraction by the phenol/chloroform method, followed by PCR amplification. The amplification products were analyzed by electrophoresis in 8% acrylamide gel. The oligonucleotide primers used yielded an amplification fragment of 400 base pairs from the bacterial DNA. Positive amplification was obtained even for the 101 bacteria/mL dilution. PCR proved to be an efficient method for the detection of H. somni. The results obtained in this study have brought relevant information for the diagnosis of H. somni, justifying the need for the diagnosis of this bacterium in bulls, especially in semen samples that should be free of contamination. The PCR method has shown to be a useful tool for the quality control of semen produced in artificial insemination centers.

Carcinoma de células escamosas em assoalho vaginal de um bovino nelore associado a metástase em pulmão: relato de caso

The squamous cell carcinoma (SCC) is a neoplasm that affect pets and production animals and it’s very common in tropical countries like Brazil; develops in sparsely pigmented, stratified squamous epithelium and in mucosal surfaces exposed Ultraviolet action. The SCC is quite infiltrative but rarely causes metastases. Its occurrence in the female reproductive tract is recognized in the literature on cattle breeds from Europe. This case was a female bovine, Nelore, adult, who was referred to the "Hospital Veterinário Luiz Quintiliano de Oliveira" with dark brown fluid leakage and putrid odor, associated with ulcerative growth of the vagina. Because the extent of injury, the animal was euthanized and taken to the necropsy, which was observed on board structure, ulcerated and pus in the vaginal floor, infiltrated into the pelvic cavity to the serosa of the uterine body. The microscopic findings were detected neoplastic proliferation of epithelial cells in the floor and vaginal metastases in the lung parenchyma, and classified the primary tumor and metastasis as squamous cell carcinoma moderately differentiated.

Adição de plasma bovino em salmouras para injeção de coxão duro bovino (m. Biceps femoris) e seus efeitos no pH e na carga microbiana de bifes cozidos embalados à vácuo e mantidos sob refrigeração

The physical (pH) and microbiological (psychotrofi c microorganisms and lactic bacteria) characteristics of beef outside round (m. Biceps femoris) injected (15%) with brines free of polyphosphates containing and sodium lactate or sodium lactate and sodium diacetate and liquid bovine plasma (PLL and PLO) or dehydrated bovine plasma (PDL, PDO) were evaluated along with beef cuts injected with brines free from plasma, but containing polyphosphates and bacteriostatic agents (CL and CO) and non injected beef cuts (IN), comprising seven treatments of cooked and vacuum packaged beef steaks stored under refrigeration (6ºC) during 43 days. No differences in pH were detected among raw or cooked injected treatments, although IN showed lower pH value in raw beef cuts. The addition of liquid or dehydrated bovine plasma did not affect the microbial load after whole muscles pasteurization, but increased the bacterial counts in cooked beef steaks during refrigerated storage, comparing to treatments with no plasma addition (CL and CO). The storage temperature (6ºC), usually found during commercialization of meat increased the microorganisms growth rate affecting the microbiological quality, especially when plasma was added to the brine.

Uso de aptâmeros na sexagem de sêmen bovino

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Quantificação do fator de crescimento semelhante a insulina I (IGF-I) em plasma bovino por ELISA

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Prospecção de genes na microbiota do rúmen bovino com propriedades degradadoras da biomassa vegetal

Pós-graduação em Microbiologia Agropecuária - FCAV

Criopreservación de semen bovino de la cola del epididimo utilizando los métodos convencional y automatizado

Cryopreservation of sperm is important to preserve the germplasm from animals of genetic value, which can die unexpectedly. This study compares conventional and automated methods of cryopreservation of spermatozoa obtained from the epididymis of bulls post-mortem. Twenty-two epididymides were obtained from a commercial slaughterhouse. Spermatozoa were collected from the tail of the epididymis using the retrograde flow technique. Thus, the samples, which were diluted in 10 ml of extender without glycerol (Botubov® I, Botupharma, Botucatu, SP, Brazil), were evaluated on motility, sperm vigor, structural and functional (swelling hypoosmotic test) membrane integrity, mitochondrial activity, sperm viability and ADN fragmentation. The samples were divided into two aliquots and diluted in extender with glycerol (Botubov® II, Botupharma, Botucatu, SP, Brazil) at a concentration of 50x106 motile sperm/0.5 French straws. One sample was frozen by the conventional method (4 hours at 5°C, in a refrigerator and 20 min in nitrogen vapor) and the other by the automated method (Cryogen® Dualflex, Neovet, Uberaba, MG, Brazil). The parameters were higher in all the tests of fresh sperm samples, with the exception of the swelling hypoosmotic test, which showed no significant difference when the results were compared with sperm frozen by the conventional method. The average motility of fresh spermatozoa was 74%, and conventional and automated averages were 29 and 25%, respectively. Therefore, although cryopreservation techniques reduce sperm quality parameters, the viability of the sperm is maintained, and these methods can be used to preserve sperm.