Potencial alelopático de extrato etanólico de Anadenanthera macrocarpa e Astronium graveolens

The objective of this study was to evaluate the allelophatic potential ethanol extract of leaves of Anadenanthera macrocarpa (Benth.) Brenan (angico) and Astronium graveolens Jacq (aroeira) on the biomonitor germination of Brassica chinensis (Malaysia's cabbage) and Lactuca sativa (lettuce). For the test of allelopathy was the bioassay of germination of B. chinensis and L. sativa using solutions of ethanol extracts in different concentrations (5, 10 and 20 mg / mL) with continuous light for 11 days. The experimental and control groups were divided into the Petri dish containing 50 seeds of B. chinensis and L. sativa each. Were performed six replicates for each concentration of extract of leaves of A. macrocarpa and A. graveolens and the negative control (water). The rolling action of the treatments was performed by quantitative observation and monitoring of germination every six hours. The results show that the ethanol extract of A. macrocarpa and A. graveolens presents allelopathic potential, regardless of the concentration used, but with answers dose-dependent, and thus able to interfere directly in germination, average speed and time of germination biomonitor of B. chinensis and L. sativa.

Inquérito soroepidemiológico sobre a infecção chagásica em doadores de sangue na região do Alto Paranaíba, Minas Gerais

This study aimed to evaluate the seroprevalence of Trypanosoma cruzi infection in blood donors of the core of Patos de Minas Regional Foundation's Hemominas, MG. To this end, tests were used (ELISA) and Reverse Passive Hemagglutination (HPR). Through retrospective study from 1996 to 2006, was considered the result of serological 21,787 donors where 259 (1.2%) were seropositive for T. cruzi infection and the highest number of seropositivity was found in the group of 41-50 years. The total number of donors tested, 15 143 (69.5%) were male and 6644 (30.5%) females giving a seroprevalence rate of 170 (0.8%) and 89 (0.4%) respectively. This seroepidemiological survey showed that still prevail rates of transmission of Chagas disease, which reinforces the idea of the need for serological tests for the prevention of Chagas disease, especially in endemic areas as the region of Triangulo Mineiro and Alto Paranaiba.

Ocorrência de mastite bovina causada por Staphylococcus sp., Streptococcus sp. E Candida sp. em uma propriedade rural no município de Indianópolis – Minas Gerais

The objective of this study was to evaluate the occurrence of bovine mastitis by Staphylococcus sp., Streptococcus sp. and Candida sp. in a rural area of Indianopolis, Minas Gerais. It was realized the California Mastitis Test (CMT) in six collect, a total 671 of milk sample positive. Then the microbiological examination was performed, where the results revealed the presence of 137 milk samples with microbial multiplication. These, showed the presence of Staphylococcus aureus (45.2% of strains), other coagulase negative Staphylococcus (10.2%), Staphylococcus epidermidis (9.4%), Staphylococcus simulans (5.8%), other coagulase negative (15.3%), Streptococcus agalactiae (7.2%), other Streptococcus sp. (5.1%) and yeasts (1.4%). It was found that 100% of Staphylococcus were susceptible to rifampicin, gentamicin and ciprofloxacin; but, resistant to penicillin, tetracycline, and oxacillin. Regarding antimicrobial susceptibility Streptococcus, were employed, except to clindamycin, erythromycin and tetracycline. We conclude that there is a great necessity of proper hygiene practices and taking prophylactic measures taken in order to reduce the infection of animals caused by infectious microorganisms and resistance.

Distribuição de poros e densidade de latossolos submetidos a diferentes sistemas de uso e manejo

Man cultivates the soil for centuries, but the intensive business and use of the soils under Cerrado vegetation for agricultural production grew out of the seventies. The objective of this study was to evaluate soil physical characteristics as a function of sampling time and the soil uses in a Cerrado area in Uberlandia City - MG, Brazil. The managements were adopted: degraded pasture (M-1), conventional tillage (M-2), minimum tillage (M-3), tillage absence (M-4), no-tillage (NT) for three years (M-5); NT for nine years (M-6), NT for three years after Pinus (M-7), PD for one year after Pinus (M-8) and Pinus forest (M-9) with 25 years old. The evaluations were conducted in 2002/03 growing season, in two areas. The soils were: area 1, an Oxisol (Red Latosol - LV-1, M-1 through M-5) and area 2, two Oxisols (Red Latosol and Red-Yellow Latosol - LVA and LV-2, M-6 through M-9). The physical attributes studied changed depending of the soil class, sampling time and management systems, with emphasis on the area 2 soils, which, in general, better preserved its main physical attributes. Managements with intense tillage, such as the M-2, are the most soil physically degrade, presenting mostly negative changes to soil bulk density, total porosity, microporosity and macroporosity. Since the systems which promote less tillage, in short term, to preserve desirable physical attributes. The M-9 system had the lowest attributes range, compared to the others.

Adubação organomineral e biofertilização líquida na produção de frutos de pinheira ( Annona squamosa L.) no submédio São Francisco, Brasil

The organic production systems based on natural processes do not allow the use of synthetic fertilizers with a high solubility, so it is required the use of technologies in accordance with the organic production legislation in order to make the technical, economical and environmental sustainable activities viable. To evaluate the effects of the usage of mineral and organic natural fertilizers, associated with liquid biofertilizers (BLE= enriched liquid biofertilizer and BF= leaf biofertilizers) on the production of the sugar apple fruit crop Annona squamosa L., replacing totally the chemical fertilizers, used by conventional farming systems, a nine year old orchard was driven in the irrigated plot n degrees 1295, irrigated project Nilo Coelho, district of Petrolina - PE. The experiment was carried out from September 2005 to February 2006. The experimental design was done through randomized blocks with eight treatments and four replications in plots of three plants each, with 96 plants in total. The treatments were the following: T(1) - plants with no fertilizer; T(2) - 60 g de N + 32 g de P(2)O(5) + 48 g de K(2)O; T(3) - 90g de N + 32 g de P(2)O(5) + 48 g de K(2)O; T(4) - 30 g de N + 32 g de P(2)O(5) + 48 g de K(2)O + 15 L de BLE + BF a 5 %; T(5) - 60g de N + 32 g de P(2)O(5) + 48 g de K(2)O + 30 L de BLE + BF a 5 %; T(6)- 90g de N + 32 g de P(2)O(5) + 48 g de K(2)O + 45 L de BLE + BF a 5 %; T(7) - 120g de N + 32 g de P(2)O(5) + 48 g de K(2)O + 60 L de BLE + BF a 5 %; T(8) - 150 g de N + 32 g de P(2)O(5) + 48 g de K(2)O + 75 L de BLE + BF a 5 % The fruit growth analyses (diameter and average length), total yield, productivity, average fruit weight, average number of fruits, fresh weight of the skin and pulp and dry weight of the fruits were used to evaluate the experiment. The results were relevant to the total production, fresh weight of the skin and dry weight of the fruits. The use of organominerals associated with liquid fertilizers increased significantly the productivity in values ranging from 10.44% (T(5) = 1.98 t ha(-1)) to 24.52 % (T(7) = 12.34 %). The treatment T(3) stood out (90g de N + 32g de P(2)O(5) + 48g de K(2)O) with a higher production than the control. The fruit weight increased in the fertilized treatments, with values increasing from treatment (T(2) = 5.26 %) to the treatment (T(7) = 12.34 %) in relation to the control. The fruit development was characterized by an average growth increase of 82 % in length and 84 % in diameter until the 56(th) day, with a sigmoidal growth pattern.

Development, characterization and clinical use of a biodegradable composite scaffold for bone engineering in oro-maxillo-facial surgery

We have developed a biodegradable composite scaffold for bone tissue engineering applications with a pore size and interconnecting macroporosity similar to those of human trabecular bone. The scaffold is fabricated by a process of particle leaching and phase inversion from poly(lactide-co-glycolide) (PLGA) and two calcium phosphate (CaP) phases both of which are resorbable by osteoclasts; the first a particulate within the polymer structure and the second a thin ubiquitous coating. The 3-5 mu m thick osteoconductive surface CaP abrogates the putative foreign body giant cell response to the underlying polymer, while the internal CaP phase provides dimensional stability in an otherwise highly compliant structure. The scaffold may be used as a biomaterial alone, as a carrier for cells or a three-phase drug delivery device. Due to the highly interconnected macroporosity ranging from 81% to 91%, with macropores of 0.8 similar to 1.8 mm, and an ability to wick up blood, the scaffold acts as both a clot-retention device and an osteoconductive support for host bone growth. As a cell delivery vehicle, the scaffold can be first seeded with human mesenchymal cells which can then contribute to bone formation in orthotopic implantation sites, as we show in immune-compromised animal hosts. We have also employed this scaffold in both lithomorph and particulate forms in human patients to maintain alveolar bone height following tooth extraction, and augment alveolar bone height through standard sinus lift approaches. We provide a clinical case report of both of these applications; and we show that the scaffold served to regenerate sufficient bone tissue in the wound site to provide a sound foundation for dental implant placement. At the time of writing, such implants have been in occlusal function for periods of up to 3 years in sites regenerated through the use of the scaffold.

Reproductive modes in frogs and their unexpected diversity in the Atlantic forest of Brazil

Amphibians, and especially the anurans (frogs and toads), exhibit a greater diversity of reproductive modes than other tetrapod vertebrates. Twenty-nine reproductive modes have been recognized for the anurans; we propose 10 more, elevating by more than 34% the number of reproductive modes known for anurans worldwide. These newly recognized reproductive modes for the frogs elevate by almost 48% the number of anuran reproductive modes known for the Neotropics. The highly complex topography of the Atlantic forest, breaking up the biome into many small microhabitats, and the high humidity, which reduces desiccation risks, have enabled the evolution of reproductive specializations such as eggs or tadpoles that develop out of water. Nearly 90% of the Atlantic forest has been cleared, and because several anurans are endemic to this region or have specialized reproductive modes dependent on the forest, this partly explains the generalized population declines and large numbers of species that have disappeared in the last few decades. © 2005 American Institute of Biological Sciences.

Estatística de levantamentos epidemiológicos sobre cárie dentária, publicados no brasil e no exterior, de 1960 a 2001

The epidemiological surveys are important to the deployment, implementation and evaluation of projects and health actions in a community. The planning, goals, samples, team training/calibration, carrying out and publication of results are extremely important in the epidemiological surveys. Thus, the care with the sample and statistical analysis is fundamental for the results to be consistent and trustworthy in order to be able to be inferred for all the population. The aims of this study is to investigate the statistical methodology used in papers on dental caries epidemiological surveys published from 1960 to 2001. A bibliographical survey was carried out in BBO, MEDLINE and SCIELO databases. The papers found were analyzed with regards to the statistical methodology applied in the whole study, from the sampling to the tabling of data. Most studies (72.6%) only presented the number of elements that composed the sample, without explaining the planning involved in obtaining it.

Gaseous neurotransmitters and their role in anapyrexia

Mammals keep their body temperature (Tb) relatively constant even under a wide range of ambient temperature variation. However, in some particular situations it may be beneficial to increase or to decrease Tb. For instance, under hypoxic conditions, a regulated drop in Tb (anapyrexia) takes place which has been reported to be crucial for survival in a number of different species. This review highlights major advances in the research about nitric oxide (NO) and carbon monoxide (CO-where data are relatively less abundant), before focusing on the role played by these gaseous neuromediators in thermoregulation, under the conditions of euthermia and anapyrexia. Available data are consistent with the notion that both NO and CO, acting on the CNS, participate in thermoregulation, with NO decreasing Tb and CO increasing it. However further studies are required before definitive conclusions can be made as to their physiological mechanisms of action.

Exercise at anaerobic threshold intensity and insulin secretion by isolated pancreatic islets of rats

To evaluate the effect of acute exercise and exercise training at the anaerobic threshold (AT) intensity on aerobic conditioning and insulin secretion by pancreatic islets, adult male Wistar rats were submitted to the lactate minimum test (LMT) for AT determination. Half of the animals were submitted to swimming exercise training (trained), 1 h/day, 5 days/week during 8 weeks, with an overload equivalent to the AT. The other half was kept sedentary. At the end of the experimental period, the rats were submitted to an oral glucose tolerance test and to another LMT. Then, the animals were sacrificed at rest or immediately after 20 minutes of swimming exercise at the AT intensity for pancreatic islets isolation. At the end of the experiment mean workload (% bw) at AT was higher and blood lactate concentration (mmol/L) was lower in the trained than in the control group. Rats trained at the AT intensity showed no alteration in the areas under blood glucose and insulin during OGTT test. Islet insulin content of trained rats was higher than in the sedentary rats while islet glucose uptake did not differ among the groups. The static insulin secretion in response to the high glucose concentration (16.7 mM) of the sedentary group at rest was lower than the sedentary group submitted to the acute exercise and the inverse was observed in relation to the trained groups. physical training at the AT intensity improved the aerobic condition and altered insulin secretory pattern by pancreatic islets. © 2010 Landes Bioscience.

Estabelecimento de fungos nematófagos aplicados no campo para o controle dos nematoides-chaves dos citros no Brasil

Monitoring the survival of nematophagous fungi is needed to establish periods of reapplication of formulations of nematophagous fungi to control the citrus nematode in the field. We monitored the survival of fungi: Arthrobotrys robusta, A. oligospora, A. musiformis Dactylella leptospora and Monacrosporium eudermatum in plots treated with 1, 2, 4, 6 liters of the formulation of fungi/plant or witness without the application, during the period of nine months with the first assessment six months after application and the other with intervals of three months after the first evaluation. The fungus D. leptospora was found only in the evaluation of 6 months after treatment application, indicated a short survival time in the soil. However, the isolated A. robusta, A. musiformis and A. oligospora were recovered in all evaluations and especially in plots treated with higher doses of the formulation and witness. Monacrosporium eudermatum was recovered in all experimental periods and even in assessing the witness portion of nine months after application. The fact of the presence of species of Arthrobotrys and M. eudermatum in control plots indicates that native species that were already orchard.

Efeito de cultivares de repolho e doses de extrato aquoso de nim na alimentação e biologia de Plutella xylostella (linnaeus) (lepidoptera: Plutellidae)

The isolated and associated effects of cabbage cultivars Ruby Ball and Chato de Quintal and aqueous extract of seeds of fruits of neem (Azadirachta indica A. Juss.) were evaluated in the concentrations of 5 and 10% on Plutella xylostella (L.) attractiveness, feeding and development. In free-choice no-preference for feeding test, leaf discs of each treatment were placed in Petri dishes where three caterpillars/treatment were released while in no-choice test two caterpillars were placed in Petri dishes with one leaf disc in each dish. In the biology test, neonate larvae were transferred to Petri dishes in the proportion of one per dish, whereas the biological parameters were evaluated: larvae mortality after 1, 3 and 5 days; pupae mortality; total mortality; six day-old larvae weight; 24 hour-old pupae; and adult longevity. Cultivar Ruby Ball showed lower attractiveness to the caterpillars in free-choice test. Neem extract in both concentrations was repellent to the caterpillars in free-choice test. There were no differences in the dry mass consumed by the caterpillars in free-choice and no-choice tests. There were no differences in larval, pupal and total mortality between the cultivars. Both doses of neem extract were equally efficient in the mortality of the diamondback moth. There were no differences in caterpillars and pupae weight and in the longevity of the adults of P. xyostella between the cultivars.

Análise química da folha d de abacaxizeiro cv. smooth cayenne antes e após a indução floral em função de doses e parcelamentos de nitrogênio

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Absorção de nitrogênio pelo algodoeiro herbáceo em dois sistemas de cultivo

The study of nutrient uptake is of fundamental importance to plant nutrition, as well as tell the time (growth stage) being absorbed by the culture, also signals to the levels of fertilizer to be used. Aiming to establish the uptake of nitrogen for cultivation of cotton (Gossypium hirsutum L. r. latifolium Hutch.) led to an experiment in a Alfissol under randomized block design with two treatments, sowing conventional tillage and after tillage on the straw of previous crop (millet), with five replicates. Samples were taken according to the phenology and plants development. The dry matter accumulation in cotton is linear and increasing until the period of large boll, being more intense with the appearance of flowers and boll, or 50 to 100 days after emergence. The nitrogen content is higher in newly emerged plants, having peaks at the beginning of the appearance of squares and flowers, and from the appearance of small boll, the nitrogen content in the plant decreases linearly. The extraction of nitrogen is bigger from the appearance of flowers (55-60 days after emergence).