Mass spectrometric characterization of two novel inflammatory peptides from the venom of the social wasp Polybia paulista

The social wasp P. paulista is relatively common in southeast Brazil causing many medically important stinging incidents. The seriousness of these incidents is dependent on the amount of venom inoculated by the wasps into the victims, and the characteristic envenomation symptoms are strongly dependent on the types of peptides present in the venom. In order to identify some of these naturally occurring peptides available in very low amounts, an analytical protocol was developed that uses a combination of reversed-phase and normal-phase high-performance liquid chromatography (HPLC) of wasp venom for peptide purification, with matrix-assisted laser desorption/ionization time-of-flight post-source decay mass spectrometry (MALDI-Tof-PSD-MS) and low-energy collision-induced dissociation (CID) in a quadrupole time-of-flight tandem mass spectrometry (QTof-MS/MS) instrument for peptide sequencing at the sub-picomole level. The distinction between Leu and Ile was achieved both by observing d-type fragment ions obtained under CID conditions and by comparison of retention times of the natural peptides and their synthetic counterparts (with different combinations of I and/or L at N- and C-terminal positions). To distinguish the isobaric residues K and Q, acetylation of peptides was followed by Q-Tof-MS analysis. The primary sequences obtained were INWLKLGKMVIDAL-NH2 (MW 1611.98Da) and IDWLKLGKMVMDVL-NH2 (MW 1658.98Da). Micro-scale bioassay protocols characterized both peptides as presenting potent hemolytic action, mast cell degranulation, and chemotaxis of poly-morphonucleated leukocyte (PMNL) cells. The primary sequences and the bioassay results suggest that these toxins constitute members of a new sub-class of mastoparan toxins, directly involved in the occurrence of inflammatory processes after wasp stinging. Copyright (C) 2004 John Wiley Sons, Ltd.

Protonectin (1-6): A novel chemotactic peptide from the venom of the social wasp Agelaia pallipes pallipes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Male behavior in colonies of the social wasp Polistes lanio (Hymenoptera, Vespidae)

Sixteen post-emergent colonies of Polistes lanio were studied while producing males in the course of the colonial cycle. Individually, they remained in the nest only 10.5 days (5-31, n=165). Twelve different male behaviors were observed: remaining immobile on the nest (82,8%), giving alarm (4,8%), flying out from the nest (2,4%), copulating on the nest (2,4%), being dominated (1,6%), self-grooming (1,2%), checking cells (1,2%), adult-adult trophallaxis (receiving food) (0,8%), larva-adult trophallaxis (0,8%), chewing prey and giving it to the larvae (0,8%), returning to the nest without food (0,8%), and fanning the nest (0,4%). In comparison to the behavioral repertory of females (28 items), they performed fewer tasks and remained immobile most of the time on the nest. Their behavior was largely related to self maintenance, but also included giving chewed prey to the larvae, giving alarm signals and fanning the nest.

Macromolecular array patterns of silk gland secretion in social Hymenoptera larvae

The cocoon, produced by most holometabolous insects, is built with silk that is usually produced by the larval salivary gland. Although this silk has been widely studied in the Lepidoptera, its composition and macromolecular arrangement remains unknown in the Hymenoptera. The macromolecular array patterns of the silk in the larval salivary gland of some meliponids, wasps, and ants were analyzed with polarized-light microscopy, and they were compared with those of Bombyx mori (Lepidoptera). There is a birefringent secretion in the glandular lumen of all larvae, due to filamentous structural proteins that display anisotropy. The silk in the distal, middle and proximal regions of the secretory portion of Formicidae and Vespidae glands presented a lattice optical pattern. We found a different pattern in the middle secretory portion of the Meliponini, with a zigzag rather than a lattice pattern. This indicates that the biopolymer fibers begin their macromolecular reorganization at this glandular region, different from the Formicidae and the Vespidae, in which the zigzag optical pattern was only found at the lateral duct. Probably, the mechanism of silk production in the Hymenoptera is a characteristic inherited from a common ancestor of Vespoidea and Sphecoidea; the alterations in the pattern observed in the Meliponini could be a derived characteristic in the Hymenoptera. We found no similarity in the macromolecular reorganization patterns of the silk between the Hymenoptera species and the silkworm.

Methodology for the comparison of military expenditures

Includes bibliography

Social protection for the poor in Latin America

Includes bibliography

Digital proficiency and digital inclusion: Comparison between students of computer science, public relations and engineering

New information and communication technologies may be useful for providing more in-depth knowledge to students in many ways, whether through online multimedia educational material, or through online debates with colleagues, teachers and other area professionals in a synchronous or asynchronous manner. This paper focuses on participation in online discussion in e-learning courses for promoting learning. Although an important theoretical aspect, an analysis of literature reveals there are few studies evaluating the personal and social aspects of online course users in a quantitative manner. This paper aims to introduce a method for diagnosing inclusion and digital proficiency and other personal aspects of the student through a case study comparing Information System, Public Relations and Engineering students at a public university in Brazil. Statistical analysis and analysis of variances (ANOVA) were used as the methodology for data analysis in order to understand existing relations between the components of the proposed method. The survey methodology was also used, in its online format, as a research instrument. The method is based on using online questionnaires that diagnose digital proficiency and time management, level of extroversion and social skills of the students. According to the sample studied, there is no strong correlation between digital proficiency and individual characteristics tied to the use of time, level of extroversion and social skills of students. The differences in course grades for some components are partly due to subject 'Introduction to Economics' being offered to freshmen in Public Relations, whereas subject 'Economics in Engineering' is offered in the final semesters of Engineering and Information Systems courses. Therefore, the difference could be more tied to the respondent's age than to the course. Information Systems students were observed to be older, with access to computers and Internet at the workplace, compared to the other students who access the Internet more often from home. This paper presents a pilot study aimed at conducting a diagnosis that permits proposing actions for information and communication technology to contribute towards student education. Three levels of digital inclusion are described as a scale to measure whether information technology increases personal performance and professional knowledge and skills. This study may be useful for other readers interested in themes related to education in engineering. © 2013 IEEE.

Representação social da criança com fissura labiopalatina: intervenção por meio de leitura de história

Pós-graduação em Psicologia do Desenvolvimento e Aprendizagem - FC

A minha vida seria muito diferente se não fosse a matemática: o sentido e os significados do ensino de matemática em processos de exclusão e de inclusão escolar e social na educação de jovens e adultos

A presente investigação se configura em termos qualitativos de pesquisa como um estudo de caso dos alunos de uma escola estadual integrante do programa especial de Educação de Jovens e Adultos EJA. Tais alunos estavam matriculados na 3 e na 4 etapas do programa, cujo formato curricular corresponde às quatro últimas séries do Ensino Fundamental de 5 a 8 séries, cuja operacionalização concentra conhecimento, espaço e tempo uma vez que cada etapa é desenvolvida em um ano letivo. A escola escolhida se localiza em área geográfica de ocupação cuja comunidade vive na condição de exclusão social, justamente no entorno de duas universidades públicas. Os jovens e adultos originados desse contexto, que estudam nessa escola, vivenciaram uma trajetória escolar marcada por impedimentos de estudar, reprovações e interrupções escolares que os impediram de concluir o Ensino Fundamental. Por essas razões, me propus investigar para conhecer, no âmbito do ensino da Matemática, elementos que contribuem para a (re)inclusão escolar com sucesso desses alunos, bem como elementos que acabam por incidir na sua (re)exclusão escolar, um fenômeno que retroalimenta o processo inevitável de exclusão social desses alunos. Para tanto, assumi a construção de uma trama narrativa relativa ao contexto dessa escola, envolvendo e interagindo dialogicamente os seus sujeitos nesta pesquisa, quais sejam, alunos, professores e funcionários da escola. Considerei suas historicidades e suas interpretações dos eventos pedagógicos vividos por eles em relação ao ensino de Matemática, objetivando produzir outros sentidos, relações e nexos que respondam ao como e ao por que os elementos de análise destacados contribuem e incidem no processo de inclusão ou exclusão escolar. As análises por mim procedidas possibilitam evidenciar os termos da indiferença escolar e do despreparo docente quer pela desconsideração da história do alunado quer pela visão distorcida de currículo justo e igualdade de oportunidades na comparação com os alunos legalmente ditos vinculados ao ensino regular.

Quantification of functional abilities in Rett syndrome: a comparison between stages III and IV

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Qualidade de vida e vulnerabilidade social na percepção do idoso

According to data from the World Health Organization, the older population will grow sixteenfold from 1950 to 2025 in comparison to a fivefold population growth in the same period, which is referred to by UNO as the “Ageing Era”. This places Brazil in the sixth position in the contingent of older individuals worldwide, with a number that is higher than 32 million. Considering how topics such as quality of life and social vulnerability are important in face of the growing older population, these topics must be furthered studied so that they can be understood as important variables for both better clinical practice and scientific research. To describe the social vulnerability and evaluate the quality of life of older individuals in a population hospitalized in the internal medicine ward of Bauru State Hospital. This is a descriptive qualitative study that was conducted by means of interviews and using Bardin’s discourse analysis. The inclusion criteria used in this study were: individuals at 60 years of age who were mentally capable of answering the proposed questions. Two categories concerning Quality of Life and Social Vulnerability emerged from the interviews. The following emerged from the theme Quality of Life: “Life as something important” and subcategories that involved feeling useful in society, having a supportive family, independence, optimism and joy and survival. Also, the following emerged from the theme Social Vulnerability: “Negative recognition of older individuals in society” and subcategories that involved lack of respect, functional disability, family indifference, housing-related insecurity, an inefficient health care system and loneliness. Quality of Life and Social Vulnerability are largely discussed themes in the present scenario. In this study, it was possible to perceive that the older population needs social support, effective public... (Complete abstract click electronic access below)

Weight concerns scale applied to college students: comparison between pencil-and-paper and online formats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Comparison of Botanical Composition, Soil Carbon Content, and Root Distribution of Subirrigated Meadows in The Nebraska Sandhills

Characterizing vegetation composition, carbon/nitrogen (C/N) content of soils, and root-mass distribution is critical to understanding carbon sequestration potential of subirrigated meadows in the Nebraska Sandhills. Five subirrigated meadows dominated by cool-season (C3) graminoids and five meadows dominated by warm-season (C4) grasses were selected throughout the Nebraska Sandhills. Vegetation, soil carbon and nitrogen, and root-mass density distribution were sampled in each meadow. Meadows dominated by C3 vegetation had 12% greater (P < 0.1) yields than meadows dominated by C4 vegetation. Total root-mass density was 30% greater (P < 0.1) in C4-dominated meadows than C3-dominated meadows. Total carbon and nitrogen content was 65% and 53% greater (P < 0.1), respectively, in the A horizon of C3-dominated meadows, but was 43% and 52% greater (P < 0.1), respectively, in the C horizon of C4-dominated meadows. Although meadows dominated by C3 vegetation had more carbon in the soil profile, much of the carbon in C3-dominated meadows appeared to be recalcitrant C4 carbon from historic vegetation.

A Comparison of Adaptive Strategies and Patterns of Victimization among Homeless Adolescents and Adults

One hundred and fifty-six homeless adolescents and 319 homeless adults interviewed directly on the streets and in shelters were compared for backgrounds of abuse, adaptations to life on the streets, and rates of criminal victimization when on the streets. Homeless adolescents were more likely to be from abusive family backgrounds, more likely to rely on deviant survival strategies, and more likely to be criminally victimized. A social learning model of adaptation and victimization on the streets was hypothesized. Although the model was supported for both homeless adults and adolescents, it was more strongly supported for adolescents than adults, and for males than females regardless of age.

Multifemale nests and social behavior in Euglossa melanotricha (Hymenoptera,Apidae, Euglossini)

The nesting biology and social behavior of the euglossine bee species Euglossa melanotricha was analyzed based on the monitoring of eight nests found in man-made cavities and transferred to observation boxes. Euglossa melanotricha females usually construct their nests in cavities in the ground, in buildings, or in mounds. In this study, we present new data on the nesting biology of E. melanotricha. The process of reactivation of nests was commonly observed with one to three females participating in the reactivation. The duration of the process of reactivation ranged from 10 to 78 days (n = 31) and were longer during the rainy season. Time spent (in days) for provisioning, oviposition and closing a single cell was higher in reactivations that occurred during the dry period. 151 emergences were observed (39 males and 112 females). 90 (80.3%) of the emerged females returned to the natal nest, but only 35(38.9%) remained and actively participated in the construction and provisioning of cells. The other 55 abandoned the nests after several days without performing any work in the nest. Matrifilial nest structure was regulated by dominance-subordinate aggressive behavior among females, where the dominant female laid almost all eggs. Task allocation was recognized by behavioral characteristics, namely, agonism and oophagy in cells oviposited by other females. Euglossa melanotricha is multivoltine and its nesting is asynchronous with respect to season. Our observations suggest a primitively eusocial organization. These observations of E. melanotricha provide valuable information for comparison with other species of Euglossa in an evolutionary context.