Nuclear protein import: Ran-GTP dissociates the karyopherin alphabeta heterodimer by displacing alpha from an overlapping binding site on beta.

The alpha subunit of the karyopherin heterodimer functions in recognition of the protein import substrate and the beta subunit serves to dock the trimeric complex to one of many sites on nuclear pore complex fibers. The small GTPase Ran and the Ran interactive protein, p10, function in the release of the docked complex. Repeated cycles of docking and release are thought to concentrate the transport substrate for subsequent diffusion into the nucleus. Ran-GTP dissociates the karyopherin heterodimer and forms a stoichiometric complex with Ran-GTP. Here we report the mapping of karyopherin beta's binding sites both for Ran-GTP and for karyopherin alpha. We discovered that karyopherin beta's binding site for Ran-GTP shows a striking sequence similarity to the cytoplasmic Ran-GTP binding protein, RanBP1. Moreover, we found that Ran-GTP and karyopherin alpha bind to overlapping sites on karyopherin beta. Having a higher affinity to the overlapping site, Ran-GTP displaces karyopherin alpha and binds to karyopherin beta. Competition for overlapping binding sites may be the mechanism by which GTP bound forms of other small GTPases function in corresponding dissociation-association reactions. We also mapped Ran's binding site for karyopherin beta to a cluster of basic residues analogous to those previously shown to constitute karyopherin alpha's binding site to karyopherin beta.

The cognitive mobilization index: crises and political generations

This article shows how the cognitive mobilization index, designed for use in observing potential political participation, can be used as an indicator of the political climate that a particular society is going through. Following a discussion of the theoretical elaborations (and their working definitions) of the concept of cognitive mobilization, a longitudinal study of various European countries is used to consider the question of how political crises influence cognitive mobilization indexes and what effects they have on the political socialization process among the youngest cohorts.

Ten generations of corn breeding /

Cover title.

Seven generations of pioneers : the pioneer experiences of seven generations of Kirkpatricks, descendants of Andrew Kirkpatrick, born in Scotland about 1710 /

Recollections of Martha Kirkpatarick Williams.

Philippines freedom two generations hence : [address before audience in Academy of music, Brooklyn, November 19, 1913].

Clipping from Brooklyn daily eagle.

Tomb of Amenhotep, excavated artifact, Yuni and His Wife Renenutet, rear view, two scenes illustrating the ancient Egyptian ideal of affection and remembrance among family generations

Asyut, Egypt; painted limestone

A book of recipes covering three generations of the Farny and Wurlitzer family and the wives of present business associates.

Includes recipes from a cook book compiled by Leonie Wurlitzer and Marguerite Strobel and published in 1925 in a limited ed. for the immediate family.

Foster Grandparent Program : 25th anniversary, 1965-1990 : bridging the generations of need.

Shipping list no. 90-685-P.

Hartshorn genealogy : some descendants of Lieut. John, second son of Thomas, the immigrant : complete lines of Ryal, Ira and Asa from 7th to 12th and 13th generations : notes on allied families: Burnham, Perkins, Cooke, Pettingill /

Mode of access: Internet.

Microfilms, their use or abuse ... A challenge to those charged with preserving the records of contemporary history for future generations, the paramount remedy, a timely analysis of the problem,

Mode of access: Internet.

Three generations of English women. Memoirs and correspondence of Mrs. John Taylor, Mrs. Sarah Austin, and Lady Duff Gordon.

Mode of access: Internet.

Endura : or, three generations : a New England romance / by B. P. Moore.

Mode of access: Internet.

Succession; a comedy of the generations,

Mode of access: Internet.