947 resultados para missing
Resumo:
Filaggrin loss-of-function mutations resulting in C-terminal protein truncations are strong predisposing factors in human atopic dermatitis (AD). To assess the possibility of similar truncations in canine AD, an exclusion strategy was designed on 16 control and 18 AD dogs of various breeds. Comparative immunofluorescence microscopy was performed with an antibody raised against the canine filaggrin C-terminus and a commercial N-terminal antibody. Concurrent with human AD-like features such as generalized NFKB activation and hyperproliferation, four distinctive filaggrin expression patterns were identified in non-lesional skin. It was found that 10/18 AD dogs exhibited an identical pattern for both antibodies with comparable (category I, 3/18) or reduced (category II, 7/18) expression to that of controls. In contrast, 4/18 dogs displayed aberrant large vesicles revealed by the C-terminal but not the N-terminal antibody (category III), while 4/18 showed a control-like N-terminal expression but lacked the C-terminal protein (category IV). The missing C-terminal filaggrin in category IV strongly points towards loss-of function mutations in 4/18 (22%) of all AD dogs analysed.
Resumo:
My thesis is divided into three chapters that build on one another as they show different examples of absences in the historical setting of Beloved and how the characters try to find creative uses of language to overcome them. As I argue that language plays animportant role in a search for meaning, my first chapter is about specific absences of language in Beloved. My second chapter is about the many absent characters in Beloved, such as Baby Suggs' children and husband, Halle, Howard and Buglar. In my third chapter, I analyze entities that are either missing from the text, or which represent a loss to Baby Suggs, Sethe, and Denver
