Chinese energy and climate policies after Durban: save the Kyoto Protocol

Nowadays, one of the urgent issues regarding global climate change is to discuss the future of the second period of the Kyoto Protocol. However, the divergence of views and opinions among parties in the last Conference of the Parties of the United Nations Framework Convention on Climate Change, held in Durban in December 2011, is still large. One of the bones of contention is whether the emerging developing countries, like China, should make commitments and legally bind themselves to a Green House Gas (GHG) reduction target in near future. As the largest GHG emitting country, China and its energy and climate policies will play an important role in global climate change and will also significantly influence the other countries? policies and the global climate negotiation. In this paper, we review the current differences among parties in the Durban Conference, and we analyze the recent situation, barriers, and future policies in China. Finally we highlight the impact and potential effect of Clean Development Mechanisms in avoiding China?s barriers regarding climate change. Results show that China is making a great effort to mitigate climate change by establishing and reforming its energy and climate policies in order to achieve a low-carbon development. At the same time, more innovation and international collaboration is needed in China to achieve this goal.

La financiaci��n y la inversi��n inmobiliaria : repercusiones en el urbanismo y la arquitectura

El presente trabajo de tesis doctoral tiene por finalidad demostrar que las decisiones de financiaci��n y de inversi��n inmobiliaria en Espa��a influyen de manera determinante en la configuraci��n del proceso urban��stico y arquitect��nico. Este planteamiento inicial obliga a formular las siguientes cuestiones: - En una primera fase, la situaci��n en Espa��a del sector inmobiliario y su relaci��n con el sector financiero en el contexto de la actual crisis financiera, iniciada en el a��o 2008. - Los m��todos de an��lisis y selecci��n de inversiones y su aplicaci��n a los proyectos de inversi��n en funci��n de la naturaleza de los activos inmobiliarios. - La valoraci��n inmobiliaria para determinadas entidades financieras. - Caracter��sticas de la gesti��n financiera empresarial y de las empresas inmobiliarias. - El origen de fondos para financiar el proceso urban��stico y arquitect��nico mediante las instituciones de inversi��n colectiva inmobiliaria y el mercado inmobiliario. - La regulaci��n actual en Espa��a, a nivel estatal, del suelo como materia prima del sector inmobiliario. - La edificaci��n residencial y el mercado inmobiliario en el actual contexto econ��mico. - La posibilidad de crear en Espa��a un mercado de derivados basado en activos inmobiliarios. - C��mo repercute la actividad econ��mica, a trav��s de la inversi��n y la financiaci��n, en los activos inmobiliarios, en el urbanismo y la arquitectura. Las cuestiones precedentes se resuelven de forma sistem��tica y metodol��gica en esta tesis doctoral estructurada en tres grandes bloques (inversi��n, financiaci��n y repercusiones en el urbanismo y la arquitectura), obteniendo una serie de respuestas reflejadas en el desarrollo del presente trabajo que se sintetizan en las siguientes l��neas: - La actual crisis financiera iniciada en el a��o 2008 ha provocado en Espa��a el colapso del sector inmobiliario y una nueva concepci��n en la naturaleza de los activos inmobiliarios. El sector inmobiliario trae causa del sector financiero, en especial del cr��dito bancario. - Dependencia y vinculaci��n del sector inmobiliario espa��ol de la pol��tica monetaria europea: la incorporaci��n de Espa��a a la moneda ��nica transforma por completo el sector inmobiliario espa��ol. - Los m��todos de an��lisis y selecci��n de inversiones se conforman como instrumentos relevantes que nos permiten jerarquizar nuestros proyectos. No obstante, presentan una serie de limitaciones y dificultades de aplicaci��n pr��ctica, por lo que no deben considerarse como herramientas que nos aporten una ��nica soluci��n irrefutable. - La valoraci��n de activos inmobiliarios se constituye en un pilar b��sico que fundamenta la correcta aplicaci��n de los fondos. - La inversi��n en activos inmobiliarios puede realizarse de forma directa o indirecta. En este ��ltimo supuesto, con una influencia relevante de las innovaciones financieras surgidas en los ��ltimos a��os. - Las instituciones de inversi��n colectiva y el mercado hipotecario constituyen instituciones fundamentales capaces de captar importantes cantidades de fondos que impulsan y financian el proceso urban��stico y arquitect��nico. - El complejo y cambiante sistema jur��dico espa��ol en materia de suelo, dificulta la implementaci��n de los procesos urban��sticos y arquitect��nicos. - Tras la crisis financiera de 2008, los activos inmobiliarios tienen un comportamiento similar a otros activos en cuanto a subidas y bajadas de precios. En el actual sistema econ��mico, la especulaci��n forma parte inherente a la naturaleza de los activos inmobiliarios. - Desde una perspectiva te��rica, existe la posibilidad de crear un mercado de derivados que tenga como subyacente activos de naturaleza inmobiliaria. - Sin actividad econ��mica, el proceso urban��stico y arquitect��nico carecer�� finalmente de sentido y tender�� a desaparecer. No obstante, son las innovaciones tecnol��gicas, a nivel de producto y proceso, las principales causantes del impulso de la actividad econ��mica. - A pesar de lo expresado en los documentos urban��sticos internacionales, la transformaci��n del urbanismo y la arquitectura dependen principalmente de la actividad econ��mica y la tecnolog��a. En un segundo nivel, la inversi��n y la financiaci��n condicionan y definen el urbanismo y la arquitectura, incluso a nivel de proyecto si se pretende su materializaci��n. En base al desarrollo previo, el objetivo fundamental de esta tesis doctoral ha sido demostrar que las decisiones de financiaci��n y de inversi��n tienen una importancia capital y determinan la configuraci��n de los activos inmobiliario, del urbanismo y la arquitectura, por lo que deben ser tenidas en cuenta no s��lo en su materializaci��n sino incluso en la propia concepci��n del proceso creativo. ABSTRACT The present dissertation aims to show that real estate financing and investment decisions in Spain play a predominant role in structuring urban development and architectural solutions. The issues addressed to support that contention include: - As a preliminary study, the situation of the real estate industry in Spain and its relationship to the financial sector in the context of the 2008 financial crisis. - The methods used to analyse and select investments and their application to investment projects, by type of real estate asset. - Appraisal of certain financial institutions��� real estate asset holdings. - Characteristics of financial institution and real estate company corporate management. - Sourcing funds for financing urban development and architecture through real estate investment trusts and the real estate market. - Present nation-wide regulations on landed property in Spain as a raw material for the real estate industry. - Residential building construction and the real estate market in the present economic context. - The possibility of creating a real estate asset-based derivatives market in Spain - The impact of economic activity, through investment and financing, on real estate assets, urban development and architecture. The aforementioned issues are addressed systematically and methodically in this dissertation, which is divided into three major units: investment, financing, and impact on urban development and architecture. The conclusions drawn are summarised below. - The financial crisis that began in 2008 has induced the collapse of the Spanish real estate industry and spawned a new perception of the nature of real estate assets. The real estate industry is dependent upon the financial sector, in particular on bank loans. - The Spanish real estate industry also depends on and is related to European monetary policy: Spain���s adherence to the single currency ushered in a thorough overhaul of its real estate industry. - Investment analysis and selection methods constitute highly suitable tools for project evaluation and ranking. Nonetheless, inasmuch as their practical implementation is subject to a series of limitations and difficulties, they should not be thought able to deliver a single irrefutable solution. - Real estate asset appraisal is a mainstay to the rightful application of funds. - Real estate asset investments can be made directly or indirectly. The latter approach is heavily influenced by the financial innovations forthcoming in recent years. - Investment trusts and the mortgage market are key institutions able to raise substantial funding, thereby driving and financing urban development and architecture. - Spain���s complex and changing legal provisions on land management are an obstacle to urban development and architecture. - Since the 2008 crisis, real estate assets have behaved much like other assets in terms of rising and falling prices. In the present economic context, speculation is indivisible from real estate assets. - Theoretically speaking, a derivatives market with real estate holdings as the underlying assets lies within the realm of possibility. - In the absence of economic activity, urban development and architecture are senseless pursuits and tend to disappear. Technological innovation in products and processes are the main drivers of economic activity. - Despite the opinion expressed in international papers on the subject, the transformation of urban development and architecture depend primarily on economic activity and technology. In a second dimension, investment and financing condition and define urban development and architecture, even at the design level for projects aspiring to actual construction. Pursuant to the foregoing, the primary aim of this dissertation is to show that financial and investment decisions are of cardinal importance and determine the structure of real estate assets, urban development and architecture. They must consequently be borne in mind not only in connection with implementation, but also with conceptual design and the creative process itself. I

Security needs: Some considerations about its integration into the Self-Determination-Theory (SDT)

According to Self-determination Theory (SDT), there are only three psychological needs - competence, autonomy and relatedness - truly fundamental and essential for human being?s health and well-being, which can be satisfied by individuals while engaging in a wide variety of behaviors that may differ among individuals and be differentially manifest in different cultures. However, a number of questions have been raised about SDT?s contention that there are only those three basic psychological needs. The present study discusses the possibility that the security need should be considered as a basic need and its relation to the accepted three basic psychological needs. Using the Cultural Theory framework the degree of satisfaction of the basic needs, depending on the type of culture, is also presented.

Contribution to the improvement of communication services in nanosatellite constellations using delay and disruption tolerant networking based architectures

Esta tesis se desarrolla dentro del marco de las comunicaciones satelitales en el innovador campo de los peque��os sat��lites tambi��n llamados nanosat��lites o cubesats, llamados as�� por su forma cubica. Estos nanosat��lites se caracterizan por su bajo costo debido a que usan componentes comerciales llamados COTS (commercial off-the-shelf) y su peque��o tama��o como los Cubesats 1U (10cm*10 cm*10 cm) con masa aproximada a 1 kg. Este trabajo de tesis tiene como base una iniciativa propuesta por el autor de la tesis para poner en ��rbita el primer sat��lite peruano en mi pa��s llamado chasqui I, actualmente puesto en ��rbita desde la Estaci��n Espacial Internacional. La experiencia de este trabajo de investigaci��n me llevo a proponer una constelaci��n de peque��os sat��lites llamada Waposat para dar servicio de monitoreo de sensores de calidad de agua a nivel global, escenario que es usado en esta tesis. Es ente entorno y dadas las caracter��sticas limitadas de los peque��os sat��lites, tanto en potencia como en velocidad de datos, es que propongo investigar una nueva arquitectura de comunicaciones que permita resolver en forma ��ptima la problem��tica planteada por los nanosat��lites en ��rbita LEO debido a su car��cter disruptivo en sus comunicaciones poniendo ��nfasis en las capas de enlace y aplicaci��n. Esta tesis presenta y eval��a una nueva arquitectura de comunicaciones para proveer servicio a una red de sensores terrestres usando una soluci��n basada en DTN (Delay/Disruption Tolerant Networking) para comunicaciones espaciales. Adicionalmente, propongo un nuevo protocolo de acceso m��ltiple que usa una extensi��n del protocolo ALOHA no ranurado, el cual toma en cuenta la prioridad del trafico del Gateway (ALOHAGP) con un mecanismo de contienda adaptativo. Utiliza la realimentaci��n del sat��lite para implementar el control de la congesti��n y adapta din��micamente el rendimiento efectivo del canal de una manera ��ptima. Asumimos un modelo de poblaci��n de sensores finito y una condici��n de tr��fico saturado en el que cada sensor tiene siempre tramas que transmitir. El desempe��o de la red se evalu�� en t��rminos de rendimiento efectivo, retardo y la equidad del sistema. Adem��s, se ha definido una capa de convergencia DTN (ALOHAGP-CL) como un subconjunto del est��ndar TCP-CL (Transmission Control Protocol-Convergency Layer). Esta tesis muestra que ALOHAGP/CL soporta adecuadamente el escenario DTN propuesto, sobre todo cuando se utiliza la fragmentaci��n reactiva. Finalmente, esta tesis investiga una transferencia ��ptima de mensajes DTN (Bundles) utilizando estrategias de fragmentaci��n proactivas para dar servicio a una red de sensores terrestres utilizando un enlace de comunicaciones satelitales que utiliza el mecanismo de acceso m��ltiple con prioridad en el tr��fico de enlace descendente (ALOHAGP). El rendimiento efectivo ha sido optimizado mediante la adaptaci��n de los par��metros del protocolo como una funci��n del n��mero actual de los sensores activos recibidos desde el sat��lite. Tambi��n, actualmente no existe un m��todo para advertir o negociar el tama��o m��ximo de un ���bundle��� que puede ser aceptado por un agente DTN ���bundle��� en las comunicaciones por sat��lite tanto para el almacenamiento y la entrega, por lo que los ���bundles��� que son demasiado grandes son eliminados o demasiado peque��os son ineficientes. He caracterizado este tipo de escenario obteniendo una distribuci��n de probabilidad de la llegada de tramas al nanosat��lite as�� como una distribuci��n de probabilidad del tiempo de visibilidad del nanosat��lite, los cuales proveen una fragmentaci��n proactiva ��ptima de los DTN ���bundles���. He encontrado que el rendimiento efectivo (goodput) de la fragmentaci��n proactiva alcanza un valor ligeramente inferior al de la fragmentaci��n reactiva. Esta contribuci��n permite utilizar la fragmentaci��n activa de forma ��ptima con todas sus ventajas tales como permitir implantar el modelo de seguridad de DTN y la simplicidad al implementarlo en equipos con muchas limitaciones de CPU y memoria. La implementaci��n de estas contribuciones se han contemplado inicialmente como parte de la carga ��til del nanosat��lite QBito, que forma parte de la constelaci��n de 50 nanosat��lites que se est�� llevando a cabo dentro del proyecto QB50. ABSTRACT This thesis is developed within the framework of satellite communications in the innovative field of small satellites also known as nanosatellites (<10 kg) or CubeSats, so called from their cubic form. These nanosatellites are characterized by their low cost because they use commercial components called COTS (commercial off-the-shelf), and their small size and mass, such as 1U Cubesats (10cm * 10cm * 10cm) with approximately 1 kg mass. This thesis is based on a proposal made by the author of the thesis to put into orbit the first Peruvian satellite in his country called Chasqui I, which was successfully launched into orbit from the International Space Station in 2014. The experience of this research work led me to propose a constellation of small satellites named Waposat to provide water quality monitoring sensors worldwide, scenario that is used in this thesis. In this scenario and given the limited features of nanosatellites, both power and data rate, I propose to investigate a new communications architecture that allows solving in an optimal manner the problems of nanosatellites in orbit LEO due to the disruptive nature of their communications by putting emphasis on the link and application layers. This thesis presents and evaluates a new communications architecture to provide services to terrestrial sensor networks using a space Delay/Disruption Tolerant Networking (DTN) based solution. In addition, I propose a new multiple access mechanism protocol based on extended unslotted ALOHA that takes into account the priority of gateway traffic, which we call ALOHA multiple access with gateway priority (ALOHAGP) with an adaptive contention mechanism. It uses satellite feedback to implement the congestion control, and to dynamically adapt the channel effective throughput in an optimal way. We assume a finite sensor population model and a saturated traffic condition where every sensor always has frames to transmit. The performance was evaluated in terms of effective throughput, delay and system fairness. In addition, a DTN convergence layer (ALOHAGP-CL) has been defined as a subset of the standard TCP-CL (Transmission Control Protocol-Convergence Layer). This thesis reveals that ALOHAGP/CL adequately supports the proposed DTN scenario, mainly when reactive fragmentation is used. Finally, this thesis investigates an optimal DTN message (bundles) transfer using proactive fragmentation strategies to give service to a ground sensor network using a nanosatellite communications link which uses a multi-access mechanism with priority in downlink traffic (ALOHAGP). The effective throughput has been optimized by adapting the protocol parameters as a function of the current number of active sensors received from satellite. Also, there is currently no method for advertising or negotiating the maximum size of a bundle which can be accepted by a bundle agent in satellite communications for storage and delivery, so that bundles which are too large can be dropped or which are too small are inefficient. We have characterized this kind of scenario obtaining a probability distribution for frame arrivals to nanosatellite and visibility time distribution that provide an optimal proactive fragmentation of DTN bundles. We have found that the proactive effective throughput (goodput) reaches a value slightly lower than reactive fragmentation approach. This contribution allows to use the proactive fragmentation optimally with all its advantages such as the incorporation of the security model of DTN and simplicity in protocol implementation for computers with many CPU and memory limitations. The implementation of these contributions was initially contemplated as part of the payload of the nanosatellite QBito, which is part of the constellation of 50 nanosatellites envisaged under the QB50 project.

AVALIA����O POR MEIO DE MODELOS DE GESSO DAS ALTERA����ES DENTOESQUEL��TICAS, PROMOVIDAS PELA EXPANS��O R��PIDA DA MAXILA ASSISTIDA CIRURGICAMENTE (ERMAC)

Este estudo avaliou as altera����es produzidas nos arcos dentais superiores de pacientes submetidos �� Expans��o R��pida da Maxila Assistida Cirurgicamente (ERMAC). A amostra utilizada foi composta de 50 modelos de gesso superiores de 18 pacientes, sendo seis do sexo masculino e 12 do sexo feminino, com m��dia de idade de 23,3 anos. Para cada paciente foram preparados tr��s modelos de gesso obtidos em diferentes fases: Inicial, antes do procedimento operat��rio (T1); tr��s meses p��s-expans��o (travamento do expansor) e momento da remo����o do aparelho expansor tipo Hyrax e coloca����o da placa remov��vel de acr��lico para conten����o (T2); seis meses p��s-expans��o e momento de remo����o da placa de acr��lico (T3). O dispositivo expansor utilizado foi o disjuntor tipo Hyrax. O procedimento cir��rgico adotado foi a osteotomia lateral da maxila sem o envolvimento da l��mina pterig��ide, osteotomia da espinha nasal �� linha m��dia dental (incisivos centrais superiores), separa����o da sutura palatina mediana por meio de cinzel e separa����o do septo nasal. O in��cio da ativa����o ocorreu no terceiro dia p��s-operat��rio, sendo �� de volta pela manh�� e �� �� noite, sendo que as ativa����es seguiram crit��rios cl��nicos para o controle da expans��o. As medidas foram realizadas por meio da m��quina de medi����o tridimensional (SAC), baseando-se nas altera����es nos tr��s planos (vertical, sagital e transversal) que ocorreram nos modelos de gesso. Concluiu-se que: 1. Houve um aumento estatisticamente significante nas dist��ncias transversais em todos os grupos de dentes (de incisivos centrais at�� segundos molares) de T1 para T2, demonstrando a efetividade do tratamento. De T2 para T3 n��o houve diferen��a estatisticamente significante para nenhuma vari��vel, indicando, assim, estabilidade ap��s seis meses do t��rmino da ERMAC; 2. Houve um aumento estatisticamente significante nas inclina����es dos primeiros e segundos molares dos lados direito e esquerdo e dos segundos pr��-molares apenas do lado esquerdo, sugerindo um comportamento assim��trico dos dentes avaliados; 3. Houve um aumento na largura palatina nos intervalos analisados, com diferen��as estatisticamente significantes entre T1 x T2 e T1 x T3; 4. N��o foram observadas diferen��as estatisticamente significantes na profundidade palatina nos intervalos analisados.(AU)

AVALIA����O POR MEIO DE MODELOS DE GESSO DAS ALTERA����ES DENTOESQUEL��TICAS, PROMOVIDAS PELA EXPANS��O R��PIDA DA MAXILA ASSISTIDA CIRURGICAMENTE (ERMAC)

Este estudo avaliou as altera����es produzidas nos arcos dentais superiores de pacientes submetidos �� Expans��o R��pida da Maxila Assistida Cirurgicamente (ERMAC). A amostra utilizada foi composta de 50 modelos de gesso superiores de 18 pacientes, sendo seis do sexo masculino e 12 do sexo feminino, com m��dia de idade de 23,3 anos. Para cada paciente foram preparados tr��s modelos de gesso obtidos em diferentes fases: Inicial, antes do procedimento operat��rio (T1); tr��s meses p��s-expans��o (travamento do expansor) e momento da remo����o do aparelho expansor tipo Hyrax e coloca����o da placa remov��vel de acr��lico para conten����o (T2); seis meses p��s-expans��o e momento de remo����o da placa de acr��lico (T3). O dispositivo expansor utilizado foi o disjuntor tipo Hyrax. O procedimento cir��rgico adotado foi a osteotomia lateral da maxila sem o envolvimento da l��mina pterig��ide, osteotomia da espinha nasal �� linha m��dia dental (incisivos centrais superiores), separa����o da sutura palatina mediana por meio de cinzel e separa����o do septo nasal. O in��cio da ativa����o ocorreu no terceiro dia p��s-operat��rio, sendo �� de volta pela manh�� e �� �� noite, sendo que as ativa����es seguiram crit��rios cl��nicos para o controle da expans��o. As medidas foram realizadas por meio da m��quina de medi����o tridimensional (SAC), baseando-se nas altera����es nos tr��s planos (vertical, sagital e transversal) que ocorreram nos modelos de gesso. Concluiu-se que: 1. Houve um aumento estatisticamente significante nas dist��ncias transversais em todos os grupos de dentes (de incisivos centrais at�� segundos molares) de T1 para T2, demonstrando a efetividade do tratamento. De T2 para T3 n��o houve diferen��a estatisticamente significante para nenhuma vari��vel, indicando, assim, estabilidade ap��s seis meses do t��rmino da ERMAC; 2. Houve um aumento estatisticamente significante nas inclina����es dos primeiros e segundos molares dos lados direito e esquerdo e dos segundos pr��-molares apenas do lado esquerdo, sugerindo um comportamento assim��trico dos dentes avaliados; 3. Houve um aumento na largura palatina nos intervalos analisados, com diferen��as estatisticamente significantes entre T1 x T2 e T1 x T3; 4. N��o foram observadas diferen��as estatisticamente significantes na profundidade palatina nos intervalos analisados.(AU)

AVALIA����O POR MEIO DE MODELOS VIRTUAIS TRIDIMENSIONAIS DAS DIST��NCIAS INTERCANINOS E INTERMOLARES INFERIORES EM TR��S MOMENTOS

O prop��sito desse estudo foi de avaliar a estabilidade do tratamento ortod��ntico em tr��s momentos distintos, por meio de mensura����o das dist��ncias intercaninos e intermolares inferiores, utilizando, como ferramenta, modelos virtuais tridimensionais. Foram selecionados modelos de gesso da arcada inferior de 19 pacientes em tr��s fases distintas: pr��-tratamento (T1), p��s-tratamento (T2) e m��nimo de 5 anos p��s-conten����o (T3), totalizando 57 modelos, os quais foram digitalizados por meio do Scanner 3D SCANTEH ST500 (SCAN technology A/S, Dinamarca), para obten����o dos modelos virtuais tridimensionais. Nesses modelos virtuais tridimensionais foram selecionadas e mensuradas as dist��ncias entre as pontas de c��spide dos caninos, denominada dist��ncia intercaninos e pontas de c��spide mesiovestibulares dos primeiros molares, denominada dist��ncia intermolares, com o aux��lio do programa Rapidform ( Inus Technology Inc,). Para a an��lise estat��stica, foi realizada a an��lise de vari��ncia de medidas repetidas, e realizadas compara����es m��ltiplas de Bonferroni ao n��vel de signific��ncia de 5%. As dist��ncias intercaninos e intermolares, em m��dia, diminu��ram entre T1-T2 e continuaram diminuindo entre T2- T3. Ap��s 4 semanas, 20% da amostra foi novamente mensurada para avalia����o do erro do m��todo, onde foram encontrados valores m��dios semelhantes aos encontrados na primeira mensura����o. Nesse trabalho, utilizando-se modelos virtuais tridimensionais, nota-se que n��o h�� estabilidade durante e ap��s o tratamento ortod��ntico, no tocante ��s dist��ncias intercaninos e intermolares.

AVALIA����O POR MEIO DE MODELOS VIRTUAIS TRIDIMENSIONAIS DAS DIST��NCIAS INTERCANINOS E INTERMOLARES INFERIORES EM TR��S MOMENTOS

O prop��sito desse estudo foi de avaliar a estabilidade do tratamento ortod��ntico em tr��s momentos distintos, por meio de mensura����o das dist��ncias intercaninos e intermolares inferiores, utilizando, como ferramenta, modelos virtuais tridimensionais. Foram selecionados modelos de gesso da arcada inferior de 19 pacientes em tr��s fases distintas: pr��-tratamento (T1), p��s-tratamento (T2) e m��nimo de 5 anos p��s-conten����o (T3), totalizando 57 modelos, os quais foram digitalizados por meio do Scanner 3D SCANTEH ST500 (SCAN technology A/S, Dinamarca), para obten����o dos modelos virtuais tridimensionais. Nesses modelos virtuais tridimensionais foram selecionadas e mensuradas as dist��ncias entre as pontas de c��spide dos caninos, denominada dist��ncia intercaninos e pontas de c��spide mesiovestibulares dos primeiros molares, denominada dist��ncia intermolares, com o aux��lio do programa Rapidform ( Inus Technology Inc,). Para a an��lise estat��stica, foi realizada a an��lise de vari��ncia de medidas repetidas, e realizadas compara����es m��ltiplas de Bonferroni ao n��vel de signific��ncia de 5%. As dist��ncias intercaninos e intermolares, em m��dia, diminu��ram entre T1-T2 e continuaram diminuindo entre T2- T3. Ap��s 4 semanas, 20% da amostra foi novamente mensurada para avalia����o do erro do m��todo, onde foram encontrados valores m��dios semelhantes aos encontrados na primeira mensura����o. Nesse trabalho, utilizando-se modelos virtuais tridimensionais, nota-se que n��o h�� estabilidade durante e ap��s o tratamento ortod��ntico, no tocante ��s dist��ncias intercaninos e intermolares.

High temperature promotes auxin-mediated hypocotyl elongation in Arabidopsis

Physiological studies with excised stem segments have implicated the plant hormone indole-3-acetic acid (IAA or auxin) in the regulation of cell elongation. Supporting evidence from intact plants has been somewhat more difficult to obtain, however. Here, we report the identification and characterization of an auxin-mediated cell elongation growth response in Arabidopsis thaliana. When grown in the light at high temperature (29��C), Arabidopsis seedlings exhibit dramatic hypocotyl elongation compared with seedlings grown at 20��C. This temperature-dependent growth response is sharply reduced by mutations in the auxin response or transport pathways and in seedlings containing reduced levels of free IAA. In contrast, mutants deficient in gibberellin and abscisic acid biosynthesis or in ethylene response are unaffected. Furthermore, we detect a corresponding increase in the level of free IAA in seedlings grown at high temperature, suggesting that temperature regulates auxin synthesis or catabolism to mediate this growth response. Consistent with this possibility, high temperature also stimulates other auxin-mediated processes including auxin-inducible gene expression. Based on these results, we propose that growth at high temperature promotes an increase in auxin levels resulting in increased hypocotyl elongation. These results strongly support the contention that endogenous auxin promotes cell elongation in intact plants.

Impaired osteoclastic bone resorption leads to osteopetrosis in cathepsin-K-deficient mice

Cathepsin K is a recently identified lysosomal cysteine proteinase. It is abundant in osteoclasts, where it is believed to play a vital role in the resorption and remodeling of bone. Pycnodysostosis is a rare inherited osteochondrodysplasia that is caused by mutations of the cathepsin-K gene, characterized by osteosclerosis, short stature, and acroosteolysis of the distal phalanges. With a view to delineating the role of cathepsin K in bone resorption, we generated mice with a targeted disruption of this proteinase. Cathepsin-K-deficient mice survive and are fertile, but display an osteopetrotic phenotype with excessive trabeculation of the bone-marrow space. Cathepsin-K-deficient osteoclasts manifested a modified ultrastructural appearance: their resorptive surface was poorly defined with a broad demineralized matrix fringe containing undigested fine collagen fibrils; their ruffled borders lacked crystal-like inclusions, and they were devoid of collagen-fibril-containing cytoplasmic vacuoles. Assaying the resorptive activity of cathepsin-K-deficient osteoclasts in vitro revealed this function to be severely impaired, which supports the contention that cathepsin K is of major importance in bone remodeling.

Fidelity of G protein ��-subunit association by the G protein ��-subunit-like domains of RGS6, RGS7, and RGS11

Several regulators of G protein signaling (RGS) proteins contain a G protein ��-subunit-like (GGL) domain, which, as we have shown, binds to G��5 subunits. Here, we extend our original findings by describing another GGL-domain-containing RGS, human RGS6. When RGS6 is coexpressed with different G�� subunits, only RGS6 and G��5 interact. The expression of mRNA for RGS6 and G��5 in human tissues overlaps. Predictions of ��-helical and coiled-coil character within GGL domains, coupled with measurements of G�� binding by GGL domain mutants, support the contention that G��-like regions within RGS proteins interact with G��5 subunits in a fashion comparable to conventional G��/G�� pairings. Mutation of the highly conserved Phe-61 residue of G��2 to tryptophan, the residue present in all GGL domains, increases the stability of the G��5/G��2 heterodimer, highlighting the importance of this residue to GGL/G��5 association.

(+)-Abscisic Acid 8���-Hydroxylase Is a Cytochrome P450 Monooxygenase1

Abscisic acid (ABA) 8���-hydroxylase catalyzes the first step in the oxidative degradation of (+)-ABA. The development of a robust in vitro assay has now permitted detailed examination and characterization of this enzyme. Although several factors (buffer, cofactor, and source tissue) were critical in developing the assay, the most important of these was the identification of a tissue displaying high amounts of in vivo enzyme activity (A.J. Cutler, T.M. Squires, M.K. Loewen, J.J. Balsevich [1997] J Exp Bot 48: 1787���1795). (+)-ABA 8���-hydroxylase is an integral membrane protein that is localized to the microsomal fraction in suspension-cultured maize (Zea mays) cells. (+)-ABA metabolism requires both NADPH and molecular oxygen. NADH was not an effective cofactor, although there was substantial stimulation of activity (synergism) when it was included at rate-limiting NADPH concentrations. The metabolism of (+)-ABA was progressively inhibited at O2 concentrations less than 10% (v/v) and was very low (less than 5% of control) under N2. (+)-ABA 8���-hydroxylase activity was inhibited by tetcyclacis (50% inhibition at 10���6 m), cytochrome c (oxidized form), and CO. The CO inhibition was reversible by light from several regions of the visible spectrum, but most efficiently by blue and amber light. These data strongly support the contention that (+)-ABA 8���-hydroxylase is a cytochrome P450 monooxygenase.

Evidence for 1-(Malonylamino)cyclopropane-1-Carboxylic Acid Being the Major Conjugate of Aminocyclopropane-1-Carboxylic Acid in Tomato Fruit1

Tomato (Lycopersicon esculentum Miller) fruit discs fed with [2,3-14C]1-aminocyclopropane-1-carboxylic acid (ACC) formed 1-malonyl-ACC (MACC) as the major conjugate of ACC in fruit throughout all ripening stages, from immature-green through the red-ripe stage. Another conjugate of ACC, ��-glutamyl-ACC (GACC), was formed only in mature-green fruit in an amount about 10% of that of MACC; conjugation of ACC into GACC was not detected in fruits at other ripening stages. No GACC formation was observed from etiolated mung bean (Vigna radiata [L.] Wilczek) hypocotyls, etiolated common vetch (Vicia sativum L.) epicotyls, or pea (Pisum sativum L.) root tips, etiolated epicotyls, and green stem tissue, where active conversion of ACC into MACC was observed. GACC was, however, formed in vitro in extracts from fruit of all ripening stages. GACC formation in an extract from red fruit at pH 7.15 was only about 3% of that at pH 8.0, the pH at which most assays were run. Our present in vivo data support the previous contention that MACC is the major conjugate of ACC in plant tissues, whereas GACC is a minor, if any, conjugate of ACC. Thus, our data do not support the proposal that GACC formation could be more important than MACC formation in tomato fruit.

Molecular cloning of insect pro-phenol oxidase: a copper-containing protein homologous to arthropod hemocyanin.

Pro-phenol oxidase [pro-PO; zymogen of phenol oxidase (monophenol, L-dopa:oxygen oxidoreductase, EC 1.14.18.1)] is present in the hemolymph plasma of the silkworm Bombyx mori. Pro-PO is a heterodimeric protein synthesized by hemocytes. A specific serine proteinase activates both subunits through a limited proteolysis. The amino acid sequences of both subunits were deduced from their respective cDNAs; amino acid sequence homology between the subunits was 51%. The deduced amino acid sequences revealed domains highly homologous to the copper-binding site sequences (copper-binding sites A and B) of arthropod hemocyanins. The overall sequence homology between silkworm pro-PO and arthropod hemocyanins ranged from 29 to 39%. Phenol oxidases from prokaryotes, fungi, and vertebrates have sequences homologous to only the copper-binding site B of arthropod hemocyanins. Thus, silkworm pro-PO DNA described here appears distinctive and more closely related to arthropod hemocyanins. The pro-PO-activating serine proteinase was shown to hydrolyze peptide bonds at the carboxyl side of arginine in the sequence-Asn-49-Arg-50-Phe-51-Gly-52- of both subunits. Amino groups of N termini of both subunits were indicated to be N-acetylated. The cDNAs of both pro-PO subunits lacked signal peptide sequences. This result supports our contention that mature pro-PO accumulates in the cytoplasm of hemocytes and is released by cell rupture, as for arthropod hemocyanins.

The bacterial colicin active against tumor cells in vitro and in vivo is verotoxin 1.

We have identified verotoxin 1 (VT1) as the active component within an antineoplastic bacteriocin preparation from Escherichia coli HSC10 studied over two decades. Recombinant VT1 can simulate the toxicity of anticancer proteins (ACP), and the antineoplastic activity of ACP (and VT1) was abrogated by treatment with anti-VT1 antibody. Similarly, VT1 mimics the protective effect of ACP in a murine metastatic fibrosarcoma model. Prior immunization with VT1 B subunit prevents the effect of VT1 or ACP in this model. The activity of ACP against a variety of human ovarian cell lines was mimicked by VT1, and multidrug-resistant variants were significantly hypersensitive. Primary ovarian tumors and metastases contain elevated levels of globotriaosylceramide compared with normal ovaries, and overlay of frozen tumor sections showed selective VT binding to tumor tissue and the lumen of invading blood vessels. Our contention that VT1 could provide an additional approach to the management of certain human neoplasms is discussed.