Phytoplankton composition and biomass during leg 7 of Galathea 3 cruise across the southern Indian Ocean

Phytoplankton composition and biomass was investigated across the southern Indian Ocean. Phytoplankton composition was determined from pigment analysis with subsequent calculations of group contributions to total chlorophyll a (Chl a) using CHEMTAX and, in addition, by examination in the microscope. The different plankton communities detected reflected the different water masses along a transect from Cape Town, South Africa, to Broome, Australia. The first station was influenced by the Agulhas Current with a very deep mixed surface layer. Based on pigment analysis this station was dominated by haptophytes, pelagophytes, cyanobacteria, and prasinophytes. Sub-Antarctic waters of the Southern Ocean were encountered at the next station, where new nutrients were intruded to the surface layer and the total Chl a concentration reached high concentrations of 1.7 µg Chl a/L with increased proportions of diatoms and dinoflagellates. The third station was also influenced by Southern Ocean waters, but located in a transition area on the boundary to subtropical water. Prochlorophytes appeared in the samples and Chl a was low, i.e., 0.3 µg/L in the surface with prevalence of haptophytes, pelagophytes, and cyanobacteria. The next two stations were located in the subtropical gyre with little mixing and general oligotrophic conditions where prochlorophytes, haptophytes and pelagophytes dominated. The last two stations were located in tropical waters influenced by down-welling of the Leeuwin Current and particularly prochlorophytes dominated at these two stations, but also pelagophytes, haptophytes and cyanobacteria were abundant. Haptophytes Type 6 (sensu Zapata et al., 2004), most likely Emiliania huxleyi, and pelagophytes were the dominating eucaryotes in the southern Indian Ocean. Prochlorophytes dominated in the subtrophic and oligotrophic eastern Indian Ocean where Chl a was low, i.e., 0.043-0.086 µg total Chl a/L in the surface, and up to 0.4 µg Chl a/L at deep Chl a maximum. From the pigment analyses it was found that the dinoflagellates of unknown trophy enumerated in the microscope at the oligotrophic stations were possibly heterotrophic or mixotrophic. Presence of zeaxanthin containing heterotrophic bacteria may have increased the abundance of cyanobacteria determined by CHEMTAX.

(Table 1) Near surface water chemistry of south Patagonian waters during spring and winter

Both the biomass of autotrophic dinoflagellates and its contribution to total chlorophyll were found to increase significantly with seawater temperature and the level of stratification in southern Patagonian waters during spring and winter. The highest peak of biomass corresponded to a single species, Prorocentrum minimum (Pavillard) Schiller, and was detected in middle shelf waters, coinciding with the primary productivity and CO2 uptake maxima reported for the area under spring conditions.

Hydrochemistry measured on water bottle samples during METEOR cruise M10/1

The relationship between the vertical flux of microplankton and its standing stock in the upper ocean was determined in the subtropical (33°N, 21°W) and tropical (18°N, 30°W) northeast Atlantic in spring 1989 as part of the North Atlantic Bloom Experiment. In the subtropical area specific sedimentation rates at all depths were low (0.1% of standing stock) and 10-20% of settled particulate organic carbon (POC) was viable diatoms. The high contribution of viable diatoms, their empty frustules and tintinnid loricae to settled material characterized a system in transition between a diatom bloom sedimentation event and an oligotrophic summer situation. In the tropical area specific sedimentation rates were similar, but absolute rates (3 mg C m?2 day?1) were only about a third of those in the subtropical area. Microplankton carbon contributed only 2-6% to POC. Hard parts of heterotrophs found embedded in amorphous detrital matter suggest that particles had passed through a complex food web prior to sedimentation. Coccolithophorids, not diatoms dominated the autotrophic fraction in traps, and a shift in the composition of autotrophs may indicate a perturbation of the oligotrophic system.

Villefranche sur Mer: multistressors experiment March 2012

The effect of ocean warming and acidification was investigated on a natural plankton assemblage from an oligotrophic area, the bay of Villefranche (NW Mediterranean Sea). The assemblage was sampled in March 2012 and exposed to the following four treatments for 12 days: control ( 360 µatm, 14°C), elevated pCO2 ( 610 µatm, 14°C), elevated temperature ( 410 µatm, 17°C), and elevated pCO2 and temperature ( 690 µatm, 17°C). Nutrients were already depleted at the beginning of the experiment and the concentrations of chlorophyll a (chl a), heterotrophic prokaryotes and viruses decreased, under all treatments, throughout the experiment. There were no statistically significant effects of ocean warming and acidification, whether in isolation or combined, on the concentrations of nutrients, particulate organic matter, chl a and most of the photosynthetic pigments. Furthermore, 13C labelling showed that the carbon transfer rates from 13C-sodium bicarbonate into particulate organic carbon were not affected by seawater warming nor acidification. Rates of gross primary production followed the general decreasing trend of chl a concentrations and were significantly higher under elevated temperature, an effect exacerbated when combined to elevated pCO2 level. In contrast to the other algal groups, the picophytoplankton population (cyanobacteria, mostly Synechococcus) increased throughout the experiment and was more abundant in the warmer treatment though to a lesser extent when combined to high pCO2 level. These results suggest that under nutrient-depleted conditions in the Mediterranean Sea, ocean acidification has a very limited impact on the plankton community and that small species will benefit from warming with a potential decrease of the export and energy transfer to higher trophic levels.

Micro- and picoplankton in waters of the Saint Paul Island, Pribilof Islands, Bering Sea

On the basis of materials collected in June-August 1994 characteristic data on microplankton were gathered in three biotopes of the eastern shelf of the Bering Sea: open shelf (coastal zone), the harbor, and the salt lagoon of Saint Paul Island (Pribiof Islands). The following parameters of microplanktonic communities were analyzed: abundance, biomass, and production of autotrophic picoplankton (picoalgae and cyanobacteria); abundance, biomass, growth rate constant, and production of bacterioplankton; role of filiform bacteria in bacterioplankton; species composition of heterotrophic flagellates and ciliates, their abundance, and biomass. Growth rates and consumption rates of picoplankton and bacterioplankton by heterotrophic nano- and microplankton were estimated in the experiments using the dilution method. Temporal dynamics of all structural and functional parameters of microplankton were analyzed. The minor role of autotrophic picoplankton and significant role of bacterioplankton as well as heterotrophic nano- and microplankton in planktonic communities of studied biotopes during summer months was shown. During certain periods, bacterial biomass was as high as 50-65% of phytoplankton biomass, and production of bacteria was as high as 20-40% of primary production. In the middle of the season biomass of nano- and microheterotrophic organisms in different biotopes exceeded biomass of mesozooplankton 2-10 times. Average consumption of bacterial production by nano- and microplankton during the period of observations was 85-94%.

Results of biogeochemical studies in the Russian Arctic seas: isotope and radioisotope data

Comprehensive biogeochemical studies including determination of isotopic composition of organic carbon in both suspended matter and surface layer (0-1 cm) bottom sediments (more than 260 determinations of d13C-Corg) were carried out for five Arctic shelf seas: White, Barents, Kara, East Siberian, and Chukchi Seas. The aim of this study is to elucidate causes that change isotopic composition of particulate organic carbon at the water-sediment boundary. It is shown that isotopic composition of organic carbon in sediments from seas with high river run-off (White, Kara, and East Siberian Seas) does not inherit isotopic composition of organic carbon in particles precipitating from the water column, but is enriched in 13C. Seas with low river run-off (Barents and Chukchi Seas) show insignificant difference between d13C-Corg values in both suspended load and sediments because of low content of isotopically light allochthonous organic matter in suspended matter. Biogeochemical studies with radioisotope tracers (14CO2, 35S, and 14CH4) revealed existence of specific microbial filter formed from heterotrophic and autotrophic organisms at the water-sediment boundary. This filter prevents mass influx of products of organic matter decomposition into the water column, as well as reduces influx of OM contained in suspended matter from water into sediments.

(Table 1) Microplankton biomass determined from ATP and by microscopy

Simultaneous determinations of the microplankton biomass by direct microscopy and by measuring concentration of adenosine triphosphate (ATP) in samples from different depths of the Eastern Pacific revealed considerable differences between values obtained. With the exception of 3 of 62 determinations, the biomass determined from ATP exceeded that measured by microscopy; the latter averaged about 10% of the former for the region as a whole. This sharp difference is largely due to underestimation of ultramicroscopic cells by direct microscopy and to substantial variations in the factor used to convert ATP concentrations to cell biomass.