Human peripheral blood V alpha 24(+) V beta 11(+) NKT cells expand following administration of alpha-galactosylceramide-pulsed dendritic cells

Background and Objectives We have undertaken the first clinical trial involving the administration of alpha-GalactosylCeramine (alpha-GalCer)-pulsed dendritic cells (DCs) to human subjects, to determine safety, optimal dose, optimal administration route and immunological effects. Materials and Methods Subjects (n = 4) with metastatic malignancy received two infusions of alpha-GalCer-pulsed DCs intravenously, and two infusions intradermally. The percentages of Valpha24 Vbeta11 NKT cells in peripheral blood (PB) were determined by three-colour flow cytometry and the PB NKT cell numbers were calculated using the total number of PB lymphocytes/ml determined by automated full-blood counts. Results No serious treatment related adverse events were observed during the study period. Administration of alpha-GalCer-pulsed DCs in vivo can significantly (P < 0.03) increase PB Valpha24(+) Vbeta11(+) NKT cell numbers above pretreatment baseline levels after the transient fall in the NKT numbers within 48 h. Conclusions Administration of alpha-GalCer-pulsed DCs is well tolerated, modulates PB Valpha24(+) Vbeta11(+) NKT cells and may have a role in the therapy of malignancies sensitive to activities of Valpha24(+) Vbeta11(+) NKT cells, or for autoimmune diseases.

Matéria orgânica do solo e emissão de C-CO2 em diferentes manejos e cultivos agrícolas

O manejo do solo deve ser realizado de tal forma que garanta a produção sustentável ao longo dos anos. Dentre as técnicas empregas, o manejo agroecológico e o plantio direto favorecem a manutenção da cobertura do solo e o aporte de matéria orgânica. Partindo da hipótese de que o maior aporte de resíduos culturais aumenta o conteúdo e estoque de matéria orgânica no solo, bem como reduz a emissão de C-CO2, o objetivo geral da pesquisa foi avaliar o impacto do manejo na matéria orgânica do solo e na emissão de C-CO2, nos períodos secos e chuvosos em diferentes cultivos agrícolas. O capítulo 1 foi desenvolvido na comunidade de Feliz Lembrança, Alegre–ES, onde foram avaliados sistemas de manejo em pastagem (PAST), café a pleno sol (PS) e café em sistema agroflorestal (SAF) e uma mata nativa (MN). O capítulo 2 foi desenvolvido no Incaper de Domingos Martins, onde se avaliou tratamentos de plantio direto de hortaliças sob palhada de gramínea (PD-G), leguminosa (PD-L), consórcio gramínea/leguminosa (PD-GL) e convencional utilizando enxada rotativa no pré-plantio (PC)em um delineamento de blocos casualizados. Amostras de solos em diferentes camadas foram coletadas para caracterização química e da matéria orgânica. Foram realizadas medições de emissão de C-CO2, temperatura do solo, umidade do solo e C biomassa microbiana do solo in situ. Foi utilizada análise de variância multivariada, vinculada a teste de aleatorização e aplicação de contrastes ortogonais no capítulo 1 e análise de variância aplicando teste F e teste de médias no capítulo 2. O SAF apresentou maior conteúdo de C orgânico total (19,8 g/kg) na camada de 0 a 5 cm e a PAST em subsuperfície. O menor estoque de C e N e os maiores valores de quociente metabólico foram encontrados no PS. O SAF reduziu a emissão de C-CO2 em 1,93 Mg ha-1 ano-1 em relação ao PS. O C orgânico total variou de 34,94 a 50,48 g/kg no PD-GL enquanto no sistema PC essa variação foi de 27,11 a 43,74 g/kg no perfil amostrado. A emissão média anual foi de 15,89 Mg C-CO2 ha-1 ano-1para a PD-G enquanto o PD-GL foi de 13,77; PD-L de 13,09 e PC de 11,20 Mg C-CO2 ha-1 ano-1. No PC, o balanço de C foi negativo (-2,15Mg ha-1), além de apresentar as menores médias anuais de umidade do solo e C biomassa microbiana e maior Qmet anual. Sistemas com contínuo e diversificado aporte de matéria orgânica promovem redução na emissão de C-CO2, bem como atuam no sequestro de C atmosférico.

Descoberta de conhecimento em Logs de tentativas de intrusão. Um estudo de caso em Instituições de Ensino Superior

Perante a evolução constante da Internet, a sua utilização é quase obrigatória. Através da web, é possível conferir extractos bancários, fazer compras em países longínquos, pagar serviços sem sair de casa, entre muitos outros. Há inúmeras alternativas de utilização desta rede. Ao se tornar tão útil e próxima das pessoas, estas começaram também a ganhar mais conhecimentos informáticos. Na Internet, estão também publicados vários guias para intrusão ilícita em sistemas, assim como manuais para outras práticas criminosas. Este tipo de informação, aliado à crescente capacidade informática do utilizador, teve como resultado uma alteração nos paradigmas de segurança informática actual. Actualmente, em segurança informática a preocupação com o hardware é menor, sendo o principal objectivo a salvaguarda dos dados e continuidade dos serviços. Isto deve-se fundamentalmente à dependência das organizações nos seus dados digitais e, cada vez mais, dos serviços que disponibilizam online. Dada a mudança dos perigos e do que se pretende proteger, também os mecanismos de segurança devem ser alterados. Torna-se necessário conhecer o atacante, podendo prever o que o motiva e o que pretende atacar. Neste contexto, propôs-se a implementação de sistemas de registo de tentativas de acesso ilícitas em cinco instituições de ensino superior e posterior análise da informação recolhida com auxílio de técnicas de data mining (mineração de dados). Esta solução é pouco utilizada com este intuito em investigação, pelo que foi necessário procurar analogias com outras áreas de aplicação para recolher documentação relevante para a sua implementação. A solução resultante revelou-se eficaz, tendo levado ao desenvolvimento de uma aplicação de fusão de logs das aplicações Honeyd e Snort (responsável também pelo seu tratamento, preparação e disponibilização num ficheiro Comma Separated Values (CSV), acrescentando conhecimento sobre o que se pode obter estatisticamente e revelando características úteis e previamente desconhecidas dos atacantes. Este conhecimento pode ser utilizado por um administrador de sistemas para melhorar o desempenho dos seus mecanismos de segurança, tais como firewalls e Intrusion Detection Systems (IDS).

EMMON - EMbedded MONitoring

Despite the steady increase in experimental deployments, most of research work on WSNs has focused only on communication protocols and algorithms, with a clear lack of effective, feasible and usable system architectures, integrated in a modular platform able to address both functional and non–functional requirements. In this paper, we outline EMMON [1], a full WSN-based system architecture for large–scale, dense and real–time embedded monitoring [3] applications. EMMON provides a hierarchical communication architecture together with integrated middleware and command and control software. Then, EM-Set, the EMMON engineering toolset will be presented. EM-Set includes a network deployment planning, worst–case analysis and dimensioning, protocol simulation and automatic remote programming and hardware testing tools. This toolset was crucial for the development of EMMON which was designed to use standard commercially available technologies, while maintaining as much flexibility as possible to meet specific applications requirements. Finally, the EMMON architecture has been validated through extensive simulation and experimental evaluation, including a 300+ nodes testbed.

Visualizing control systems performance: A fractional perspective

This article presents a novel method for visualizing the control systems behavior. The proposed scheme uses the tools of fractional calculus and computes the signals propagating within the system structure as a time/frequency-space wave. Linear and nonlinear closed-loop control systems are analyzed, for both the time and frequency responses, under the action of a reference step input signal. Several nonlinearities, namely, Coulomb friction and backlash, are also tested. The numerical experiments demonstrate the feasibility of the proposed methodology as a visualization tool and motivate its extension for other systems and classes of nonlinearities.

Об одном способе накопления томографической системы при её решении методам наименьших квадратов

სტატიაში, ავტორების მიერ შემოთავაზებულია ტომოგრაფიულ განტოლებათა სისტემის ავტომატური დაგროვების ერთერთი ხერხი, სეისმიკის შებრუნებული ამოცანების ამოხსნისას უმცირეს კვადრატთა მეთოდის გამოყენებით. მეთოდიკა გამოყენებული იქნა ზოგიერთი მოდელური ამოცანის და ენგურის თაღოვანი კაშხალის მარცხენა სანაპიროს მეორე ჰორიზონტისათვის.

Remote Synchronization Reveals Network Symmetries and Functional Modules

We study a Kuramoto model in which the oscillators are associated with the nodes of a complex network and the interactions include a phase frustration, thus preventing full synchronization. The system organizes into a regime of remote synchronization where pairs of nodes with the same network symmetry are fully synchronized, despite their distance on the graph. We provide analytical arguments to explain this result, and we show how the frustration parameter affects the distribution of phases. An application to brain networks suggests that anatomical symmetry plays a role in neural synchronization by determining correlated functional modules across distant locations.

The State of the Judiciary, 2015

My job today as chief justice is to inform you and all Iowans of the condition of the Iowa Judicial Branch. The judicial branch is accountable to do its work so that Iowans can see the value of its fair and impartial courts. It is accountable every day for the resources it is given and the important responsibilities with which it has been entrusted. We best meet these obligations by becoming the best court system we can be. I am honored to report the progress the judicial branch has made towards becoming the best court system in the nation and the value of this progress for all Iowans. Both my grandfathers were carpenters. Like others who build with their hands, they could look at their work at the end of the day and see progress since the beginning of the day. Building Iowa’s court system consistent with its goals may not reveal progress at the end of every day as easily as the work of a carpenter. It is a long, careful process that requires the hands of many, working every day. But, certain days do come along when progress can be seen and our goals are closer within reach. Again this year, the goals of the judicial branch are to:  Protect Iowa’s children;  Provide full-time access to justice;  Operate an efficient, full-service court system;  Provide faster and less costly resolution of legal disputes;  Remain open and transparent; and  Provide fair and impartial justice for all.

Design, implementation and testing of DVB/MHP-application development environment

Tämä diplomityö esittelee 1.6. - 4.11.2002 Mikkelin ammattikorkeakoulun YTI-tutkimuskeskuksen Informaatio- ja mediateknologian hankekokonaisuuden STRIMT-projektin puitteissa tekemäni MHP-kehitysjärjestelmähankinnan tuloksia. Digitaalitelevision MHP-sovelluskehitysjärjestelmän hankinnan tulosten lisäksi työ antaa yleiskuvan MHP-sovelluskehitystyökaluista, digitaalitelevisiostandardeista sekä digitaalitelevisioverkkojen yhteistoimintamalleista televerkkojen kanssa. Keräämieni tietojen, tekemäni tarvemäärittelyn ja suorittamani tuotevertailun perusteella valitsin tarpeisiimme sopivimmaksi järjestelmäksi Alticast Inc.-yrityksen Altifusion-tuotteen. Tuotteen valinnassa otin huomioon myös mahdolliset tulevaisuuden tarpeet aina täysimittaista lähetysjärjestelmää varten kehittämällä vaiheittaisen hankintasuunnitelman. Järjestelmää hankittaessa tärkeintä on huomioida heti alusta alkaen mahdolliset tulevat tarpeet ja varmistaa hankinnan hyödynnettävyys myös tulevaisuudessa. Järjestelmän hankinnan lisäksi vastasin sen käyttöönotosta ja järjestelmää hyödyntävän testisovelluksen laatimisesta.

Teräspalkkirakenteiden palomitoitus hiilivetypalossa

Työn tavoitteena oli kehittää teräspalkkirakenteiden palosuunnittelua ja palosuojauksen toteutusta öljynjalostamolla käytettävien kantavien teräspalkkirakenteiden osalta. Lisäksi tavoitteena oli luoda suunnitteluohjeen runko palomitoituksen toteuttamiseksi Neste Engineering Oy:ssä. Ongelmakohtia työssä olivat rakenteiden kapasiteettien tarkka määritys, toimivien toteutusratkaisujen etsiminen, sekä öljynjalostamolla mitoituspalona käytettävän hiilivetypalon SFS-ENV-1992-1-2 käyttö yleisemmin mitoituspalona käytettävän standardipalo ISO-834 sijaan. Työssä perehdyttiin kirjallisuuden perusteella eri palosuojausmenetelmiin. Tarkemman jatkotutkimuksen kohteeksi otettiin jo käytössä hyväksi havaittu teräsputkipalkkien sisäpuoleinen betonitäyttö. Menetelmässä teräsputkipalkin oletetaan kantavan kuormat normaalitilassa ja sisällä olevan raudoitetun betonin palossa. Palkkirakenteiden kapasiteettimitoitus määritettiin laskennallisesti poikkileikkauksille. Mitoitus perustuu palkissa tapahtuvien sisäisten venymien ja puristumien tarkasteluun, sekä poikkileikkauksen tarkan lämpötilajakauman huomioimiseen. Raudoitustankojen ankkurointia palkki-pilari-liitoksessa kehitettiin valmistuksen kannalta yksioikoisemmaksi ja helpommin toteutettavaksi. Palkkien raudoituksiin suunniteltiin kierremuhvijatkoksella toteutettava ankkurointimenetelmä, jolla palkkien raudoitustangot saadaan ankkuroitua täydestä kapasiteetistaan tapauskohtaisesti pilarin vastakkaisella puolella olevaan palkkiin tai ankkurointikappaleella pilariin. Teräsputkipilarin betonivalun vaihtoehtoisiin menetelmiin tutustuttiin. Pilarin alapäähän asennettavan venttiilin läpi tapahtuva täyttö helpottaa betonointityövaihetta. Tutkimuksen tuloksena luotiin suunnitteluohjeen runko, jonka pohjalta voidaan tehdä lopullinen ohje. Myös työn tuloksena saatu laskentaohjelma palkkien momentti-kapasiteetin ja pilarin nurjahduskuorman laskemiseksi helpottaa suunnittelua. Raudoituksen ankkurointiin ja betonointiin esitettyjen menetelmien toimivuus on syytä kokeilla käytännössä ja tehdä jatkokehitys näistä saatavien kokemusten pohjalta.

Antennimittausjärjestelmän toteutus ja ominaisuuksien määrittäminen

Diplomityössä tarkasteltiin antennien säteilykuviomittauksiin käytettävien antennimittaratojen ominaisuuksia. Sovelletun elektroniikan laboratorion antennimittaradasta kehitettiin toimiva säteilykuvion mittausjärjestelmä, johon kuuluvat radiokaiuton kammio, suuntauskoneisto ja antennikannakkeet, HP 8720D -piirianalysaattori ja RF-kaapelointi sekä valmistutetut mitta-antennitorvet. Työssä vertailtiin koaksiaalikaapeleita ja valittiin antennimittaukseen soveltuvat. 1,70–2,60 GHz:n taajuusalueen mittastandardiksi suunniteltiin torviantenni, jonka kaksi prototyyppiä valmistutettiin konetekniikan osastolla. Torviantennien ominaisuudet mitattiin. Antennimittaradan ominaisuudet ja soveltuvuus erilaisiin antennimittauksiin selvitettiin sekä teoreettisesti että mittauksin.

Dynamic Analysis Model of Spherical Roller Bearings with Defects

Rolling element bearings are essential components of rotating machinery. The spherical roller bearing (SRB) is one variant seeing increasing use, because it is self-aligning and can support high loads. It is becoming increasingly important to understand how the SRB responds dynamically under a variety of conditions. This doctoral dissertation introduces a computationally efficient, three-degree-of-freedom, SRB model that was developed to predict the transient dynamic behaviors of a rotor-SRB system. In the model, bearing forces and deflections were calculated as a function of contact deformation and bearing geometry parameters according to nonlinear Hertzian contact theory. The results reveal how some of the more important parameters; such as diametral clearance, the number of rollers, and osculation number; influence ultimate bearing performance. Distributed defects, such as the waviness of the inner and outer ring, and localized defects, such as inner and outer ring defects, are taken into consideration in the proposed model. Simulation results were verified with results obtained by applying the formula for the spherical roller bearing radial deflection and the commercial bearing analysis software. Following model verification, a numerical simulation was carried out successfully for a full rotor-bearing system to demonstrate the application of this newly developed SRB model in a typical real world analysis. Accuracy of the model was verified by comparing measured to predicted behaviors for equivalent systems.

Nanoparticle-Assisted Immunoassays for Point-of-Care Testing - With Specific Interest in Minimally Interference-Prone Assays for Cardiac Troponin I

Cardiac troponins (cTn) I and T are the current golden standard biochemical markers in the diagnosis and risk stratification of patients with suspected acute coronary syndrome. During the past few years, novel assays capable of detecting cTn‐concentrations in >50% of apparently healthy individuals have become readily available. With the emerging of these high sensitivity cTn assays, reductions in the assay specificity have caused elevations in the measured cTn levels that do not correlate with the clinical picture of the patient. The increased assay sensitivity may reveal that various analytical interference mechanisms exist. This doctoral thesis focused on developing nanoparticle‐assisted immunometric assays that could possibly be applied to an automated point‐of‐care system. The main objective was to develop minimally interference‐prone assays for cTnI by employing recombinant antibody fragments. Fast 5‐ and 15‐minute assays for cTnI and D‐dimer, a degradation product of fibrin, based on intrinsically fluorescent nanoparticles were introduced, thus highlighting the versatility of nanoparticles as universally applicable labels. The utilization of antibody fragments in different versions of the developed cTnI‐assay enabled decreases in the used antibody amounts without sacrificing assay sensitivity. In addition, the utilization of recombinant antibody fragments was shown to significantly decrease the measured cTnI concentrations in an apparently healthy population, as well as in samples containing known amounts of potentially interfering factors: triglycerides, bilirubin, rheumatoid factors, or human anti‐mouse antibodies. When determining the specificity of four commercially available antibodies for cTnI, two out of the four cross‐reacted with skeletal troponin I, but caused crossreactivity issues in patient samples only when paired together. In conclusion, the results of this thesis emphasize the importance of careful antibody selection when developing cTnI assays. The results with different recombinant antibody fragments suggest that the utilization of antibody fragments should strongly be encouraged in the immunoassay field, especially with analytes such as cTnI that require highly sensitive assay approaches.

Model Selection in Summary Evaluation

A difficulty in the design of automated text summarization algorithms is in the objective evaluation. Viewing summarization as a tradeoff between length and information content, we introduce a technique based on a hierarchy of classifiers to rank, through model selection, different summarization methods. This summary evaluation technique allows for broader comparison of summarization methods than the traditional techniques of summary evaluation. We present an empirical study of two simple, albeit widely used, summarization methods that shows the different usages of this automated task-based evaluation system and confirms the results obtained with human-based evaluation methods over smaller corpora.

Ventilation rates in schools and pupil’s performance using computerized assessment tests

Research shows that poor indoor air quality (IAQ) in school buildings can cause a reduction in the students’ performance assessed by short-term computer-based tests; whereas good air quality in classrooms can enhance children's concentration and also teachers’ productivity. Investigation of air quality in classrooms helps us to characterise pollutant levels and implement corrective measures. Outdoor pollution, ventilation equipment, furnishings, and human activities affect IAQ. In school classrooms, the occupancy density is high (1.8–2.4 m2/person) compared to offices (10 m2/person). Ventilation systems expend energy and there is a trend to save energy by reducing ventilation rates. We need to establish the minimum acceptable level of fresh air required for the health of the occupants. This paper describes a project, which will aim to investigate the effect of IAQ and ventilation rates on pupils’ performance and health using psychological tests. The aim is to recommend suitable ventilation rates for classrooms and examine the suitability of the air quality guidelines for classrooms. The air quality, ventilation rates and pupils’ performance in classrooms will be evaluated in parallel measurements. In addition, Visual Analogue Scales will be used to assess subjective perception of the classroom environment and SBS symptoms. Pupil performance will be measured with Computerised Assessment Tests (CAT), and Pen and Paper Performance Tasks while physical parameters of the classroom environment will be recorded using an advanced data logging system. A total number of 20 primary schools in the Reading area are expected to participate in the present investigation, and the pupils participating in this study will be within the age group of 9–11 years. On completion of the project, based on the overall data recommendations for suitable ventilation rates for schools will be formulated.