Analysis of Alpha CA Genes in Early Vertebrates and ; High-Invertebrates

Carbonic anhydrases are enzymes that are ubiquitously found in all organisms that are engaged in catalyzing the hydration of carbon dioxide to form bicarbonate and proton and vice versa. They are crucial in the process of respiration, bone resorption, pH regulation, ion transport, and photosynthesis in plants. Out of the five classes of carbonic anhydrase α, β, γ, δ, ζ this study focused in the α carbonic anhydrases. This class of CAs constitute of 16 subfamilies in mammals that include 3 non-active enzymes known as Carbonic Anhydrase Related Proteins. The inactiveness of these enzymes is due to the loss of one or more Histidine residues in the active site. This thesis was conducted based on the aim of studying evolutionary analysis of carbonic anhydrase sequences from organisms spanning from the Cambrian age. It was carried out in two phases. The first phase was the sequence collection, which involved many biological sequence databases as a source. The scope of this segment included sequence alignments and analysis of the sequence manually and in an automated form incorporating few analysis tools. The second Phase was phylogenetic analysis and exploring the subcellular location of the proteins, which was key for the evolutionary analysis. Through the medium of the methods conducted with respect to the phases mentioned above, it was possible to accomplish the desired result. Certain thought-provoking sequences were come across and analyzed thoroughly. Whereas, Phylogenetics showed interesting results to bolster previous findings and new findings as well which lay bedrock for future intensified studies.

Frequency of elemental events of intracellular Ca��⁺ dynamics

The dynamics of intracellular Ca��⁺ is driven by random events called Ca��⁺ puffs, in which Ca��⁺ is liberated from intracellular stores. We show that the emergence of Ca��⁺ puffs can be mapped to an escape process. The mean first passage times that correspond to the stochastic fraction of puff periods are computed from a novel master equation and two Fokker-Planck equations. Our results demonstrate that the mathematical modeling of Ca��⁺ puffs has to account for the discrete character of the Ca��⁺ release sites and does not permit a continuous description of the number of open channels.

Sensitisation waves in a bidomain fire-diffuse-fire model of intracellular Ca��⁺ dynamics

We present a bidomain threshold model of intracellular calcium (Ca��⁺) dynamics in which, as suggested by recent experiments, the cytosolic threshold for Ca��⁺ liberation is modulated by the Ca��⁺ concentration in the releasing compartment. We explicitly construct stationary fronts and determine their stability using an Evans function approach. Our results show that a biologically motivated choice of a dynamic threshold, as opposed to a constant threshold, can pin stationary fronts that would otherwise be unstable. This illustrates a novel mechanism to stabilise pinned interfaces in continuous excitable systems. Our framework also allows us to compute travelling pulse solutions in closed form and systematically probe the wave speed as a function of physiologically important parameters. We find that the existence of travelling wave solutions depends on the time scale of the threshold dynamics, and that facilitating release by lowering the cytosolic threshold increases the wave speed. The construction of the Evans function for a travelling pulse shows that of the co-existing fast and slow solutions the slow one is always unstable.

Nel giorno XIX luglio MDCCCXLVI faustissimo e felicissimo, pel solenne ingresso di monsignore Bartolommeo de' conti Romilli alla sede vescovile di Cremona : omaggio di esultanza e d'amore.

Acquisition made accessible thanks to a 2015-2017 grant from the Council on Libraries and Information Resources.

S. Aurelii Augustini Confessiones.

Mode of access: Internet.

Libri decem explanationis Ecclesiastæ, graece primum & cum latina interpretatione ac commentariis vulgati /

"Appendix ... De aetate b. Gregorii ... dissertatio Joannis Lancea Panormitani": p. [296]-320.

Th. C. Cypriani De Oratione Dominica = St. Cyprian on the Lord's prayer /

Mode of access: Internet.

De civitate Dei : selections with notes and glossary /

A textbook for colleges, universities and seminaries.

Quae ratio inter Vitas Lysiae Dionysiacam, pseudo-Plutarcheam, Photianam intercedat, quaesivit A.Z.

Disbound Original Held in Oak Street Library Facility.

Omelie mistagogiche e catechetice /

Text of the homilies in Latin; commentary in Italian. Appendix contains Italian translation of the homilies.

A Démétrien /

Includes bibliographical references (p. [59]-65) and indexes.

Lettres ascétiques et morales /

Includes bibliographical references and index.

Augustins Briefwechsel mit Hieronymous /

Originally presented as the author's Habilitationsschrift--Regensburg, 1998.

Sancti Fulgentii episcopi Ruspensis Opera.

Mode of access: Internet.