Evidence for VirB4-mediated dislocation of membrane-integrated VirB2 pilin during biogenesis of the Agrobacterium VirB/VirD4 type IV secretion system.

Agrobacterium VirB2 pilin is required for assembly of the VirB/VirD4 type IV secretion system (T4SS). The propilin is processed by signal sequence cleavage and covalent linkage of the N and C termini, and the cyclized pilin integrates into the inner membrane (IM) as a pool for assembly of the secretion channel and T pilus. Here, by use of the substituted cysteine accessibility method (SCAM), we defined the VirB2 IM topology and then identified distinct contributions of the T4SS ATPase subunits to the pilin structural organization. Labeling patterns of Cys-substituted pilins exposed to the membrane-impermeative, thiol-reactive reagent 3-(N-maleimidopropionyl)biocytin (MPB) supported a topology model in which two hydrophobic stretches comprise transmembrane domains, an intervening hydrophilic loop (residues 90 to 94) is cytoplasmic, and the hydrophilic N and C termini joined at residues 48 and 121 form a periplasmic loop. Interestingly, the VirB4 ATPase, but not a Walker A nucleoside triphosphate (NTP) binding motif mutant, induced (i) MPB labeling of Cys94, a residue that in the absence of the ATPase is located in the cytoplasmic loop, and (ii) release of pilin from the IM upon osmotic shock. These findings, coupled with evidence for VirB2-VirB4 complex formation by coimmunoprecipitation, support a model in which VirB4 functions as a dislocation motor to extract pilins from the IM during T4SS biogenesis. The VirB11 ATPase functioned together with VirB4 to induce a structural change in the pilin that was detectable by MPB labeling, suggestive of a role for VirB11 as a modulator of VirB4 dislocase activity.

Heterogeneity and time

Although heterogeneity and time are central aspects of economic activity, it was predominantly the Austrian School of economics that emphasized these two aspects. In this paper we argue that the explicit consideration of heterogeneity and time is of increasing importance due to the increasing environmental and resource problems faced by humankind today. It is shown that neo-Austrian capital theory, which revived Austrian ideas employing a formal approach in the 1970s, is not only well suited to address issues of structural change and of accompanying unemployment induced by technical progress but also can be employed for an encompassing ecological-economic analysis demanded by ecological economics. However, complexity, uncertainty, and real ignorance limit the applicability of formal economic analysis. Therefore, we conclude that economic analysis has to be supplemented by considerations of political philosophy. Copyright 2006 American Journal of Economics and Sociology, Inc..

1,2-Bis(2-benzimidazolyl)-1,2-ethanediol, a chiral, tridentate, facially coordinating ligand

The ligand 1,2-bis(1H-benzimidazol-2-yl)-1,2-ethanediol, 1, and its methylated derivative 2 are readily synthesized from tartaric acid, and act as chiral, facially coordinating tridentate ligands, forming complexes of composition ML2 with octahedral transition metals. The copper(II) complexes show distorted 4 + 2 coordination with benzimidazoles occupying the equatorial sites and alcohol functions weakly binding in the axial sites. Nickel(II) complexes in three different states of protonation show regular octahedral geometry with the alcohols mutually cis. Deprotonation of the coordinated alcohol produces little structural change but the monodeprotonated complex forms a hydrogen bonded dimer. Magnetic measurements show the hydrogen bonded bridge to offer a pathway for weak antiferromagnetic coupling. UV-Visible spectroscopy shows the ligand to have a field intermediate between water and pyridine. The diastereoselectivity of complexation depends on the geometry: nickel(II) shows a weak preference for the homochiral complex, whereas copper(II) forms almost exclusively homochiral complexes.

The Great Moderation and the Relationship between Output Growth and Its Volatility

This study examines the effect of the Great Moderation on the relationship between U.S. output growth and its volatility over the period 1947 to 2006. First, we consider the possible effects of structural change in the volatility process. In so doing, we employ GARCH-M and ARCH-M specifications of the process describing output growth rate and its volatility with and without a one-time structural break in volatility. Second, our data analyses and empirical results suggest no significant relationship between the output growth rate and its volatility, favoring the traditional wisdom of dichotomy in macroeconomics. Moreover, the evidence shows that the time-varying variance falls sharply or even disappears once we incorporate a one-time structural break in the unconditional variance of output starting 1982 or 1984. That is, the integrated GARCH effect proves spurious. Finally, a joint test of a trend change and a one-time shift in the volatility process finds that the one-time shift dominates.

Modeling the Volatility of Real GDP Growth: The Case of Japan Revisited

Previous studies (e.g., Hamori, 2000; Ho and Tsui, 2003; Fountas et al., 2004) find high volatility persistence of economic growth rates using generalized autoregressive conditional heteroskedasticity (GARCH) specifications. This paper reexamines the Japanese case, using the same approach and showing that this finding of high volatility persistence reflects the Great Moderation, which features a sharp decline in the variance as well as two falls in the mean of the growth rates identified by Bai and Perronâs (1998, 2003) multiple structural change test. Our empirical results provide new evidence. First, excess kurtosis drops substantially or disappears in the GARCH or exponential GARCH model that corrects for an additive outlier. Second, using the outlier-corrected data, the integrated GARCH effect or high volatility persistence remains in the specification once we introduce intercept-shift dummies into the mean equation. Third, the time-varying variance falls sharply, only when we incorporate the break in the variance equation. Fourth, the ARCH in mean model finds no effects of our more correct measure of output volatility on output growth or of output growth on its volatility.

Cross-Country Evidence on Output Growth Volatility: Nonstationary Variance and GARCH Models

This paper revisits the issue of conditional volatility in real GDP growth rates for Canada, Japan, the United Kingdom, and the United States. Previous studies find high persistence in the volatility. This paper shows that this finding largely reflects a nonstationary variance. Output growth in the four countries became noticeably less volatile over the past few decades. In this paper, we employ the modified ICSS algorithm to detect structural change in the unconditional variance of output growth. One structural break exists in each of the four countries. We then use generalized autoregressive conditional heteroskedasticity (GARCH) specifications modeling output growth and its volatility with and without the break in volatility. The evidence shows that the time-varying variance falls sharply in Canada, Japan, and the U.K. and disappears in the U.S., excess kurtosis vanishes in Canada, Japan, and the U.S. and drops substantially in the U.K., once we incorporate the break in the variance equation of output for the four countries. That is, the integrated GARCH (IGARCH) effect proves spurious and the GARCH model demonstrates misspecification, if researchers neglect a nonstationary unconditional variance.

Bank Performance: Market Power or Efficient Structure?

Regulatory change not seen since the Great Depression swept the U.S. banking industry beginning in the early 1980s, culminating with the Interstate Banking and Branching Efficiency Act of 1994. Significant consolidations have occurred in the banking industry. This paper considers the market-power versus the efficient-structure theories of the positive correlation between banking concentration and performance on a state-by-state basis. Temporal causality tests imply that bank concentration leads bank profitability, supporting the market-power, rather than the efficient-structure, theory of that positive correlation. Our finding suggests that bank regulators, by focusing on local banking markets, missed the initial stages of an important structural change at the state level.

Referral patterns in optometry: Use of secondary consultation/comanagement centers by optometrists

Privately practicing health care practitioners, such as physicians, dentists, and optometrists are facing increasing competitive pressures as the health care industry undergoes significant structural change. The eye care field has been affected by this change and one result has been the establishment of consultation/comanagement centers for optometrists. These centers, staffed primarily by an ophthalmologist, serve community optometrists as a secondary ophthalmic care center and are altering the traditional optometric - ophthalmologic referral system.^ This study was designed to examine the response of optometrists to the formation of a center by measuring the amount and type of optometric participation in a center and identifying factors affecting participation. A predictive model was specified to determine the probability of center use by practitioners.^ The results showed that the establishment of a center in a community did not result in its usage by all practitioners though there were specific practice (organizational) and practitioners (decision-maker) variables that could be used to predict use. Three practice variables and four practitioner variables were found to be important in influencing center use. ^

NONLINEAR TIME SERIES/SYSTEM ANALYSIS (STATE-SPACE, DYNAMICS, INTERVENTION, RESILIENCE, KALMAN)

A discussion of nonlinear dynamics, demonstrated by the familiar automobile, is followed by the development of a systematic method of analysis of a possibly nonlinear time series using difference equations in the general state-space format. This format allows recursive state-dependent parameter estimation after each observation thereby revealing the dynamics inherent in the system in combination with random external perturbations.^ The one-step ahead prediction errors at each time period, transformed to have constant variance, and the estimated parametric sequences provide the information to (1) formally test whether time series observations y(,t) are some linear function of random errors (ELEM)(,s), for some t and s, or whether the series would more appropriately be described by a nonlinear model such as bilinear, exponential, threshold, etc., (2) formally test whether a statistically significant change has occurred in structure/level either historically or as it occurs, (3) forecast nonlinear system with a new and innovative (but very old numerical) technique utilizing rational functions to extrapolate individual parameters as smooth functions of time which are then combined to obtain the forecast of y and (4) suggest a measure of resilience, i.e. how much perturbation a structure/level can tolerate, whether internal or external to the system, and remain statistically unchanged. Although similar to one-step control, this provides a less rigid way to think about changes affecting social systems.^ Applications consisting of the analysis of some familiar and some simulated series demonstrate the procedure. Empirical results suggest that this state-space or modified augmented Kalman filter may provide interesting ways to identify particular kinds of nonlinearities as they occur in structural change via the state trajectory.^ A computational flow-chart detailing computations and software input and output is provided in the body of the text. IBM Advanced BASIC program listings to accomplish most of the analysis are provided in the appendix. ^

El desafío institucional : Las prácticas políticas no armadas de la Tendencia Revolucionaria del Peronismo en el Ministerio de Asuntos Agrarios de la provincia de Buenos Aires (1973-1974)

Este artículo analiza el proceso de incorporación de los sectores de la Tendencia Revolucionaria del Peronismo en el gobierno de Oscar Bidegain en la provincia de Buenos Aires, a partir de las elecciones del 11 de marzo de 1973. Se busca analizar tanto la trayectoria previa de los grupos que integraron la administración provincial, sus lugares específicos en el organigrama gubernamental, junto con las expectativas, los sentidos y los objetivos que guiaron la participación de militantes revolucionarios en las estructuras institucionales del Estado.Por otra parte, se analizan más específicamente las prácticas políticas desplegadas por estos militantes en el Ministerio de Asuntos Agrarios, junto a los programas de gestión impulsados, observando un repertorio heterogéneo de modalidades de acción inscriptas en un programa mayor de cambio de estructuras y construcción del socialismo nacional

La lógica de la inversión pública en infraestructura en la provincia de Buenos Aires : Entre las necesidades sociales y ¿la política?

El presente estudio se enfoca en la inversión en infraestructura en la provincia de Buenos Aires como un elemento central del cambio estructural pregonado para minimizar los diferenciales de productividad que constituyen la clave de los problemas del ciclo económico argentino. En el enfoque se asume la relación bidireccional entre territorio y escala nacional. Se diferencian para el análisis la infraestructura social, productiva y aquella que es consecuencia de la estructura económica dominante. Se incluye un estudio descriptivo del peso de cada tipo de infraestructura y de la lógica de localización de la inversión, y también se realiza una regresión econométrica mediante la cual se buscan variables explicativas significativas

El desafío institucional : las prácticas políticas no armadas de la Tendencia Revolucionaria del Peronismo en el Ministerio de Asuntos Agrarios de la provincia de Buenos Aires (1973-1974)

Este artículo analiza el proceso de incorporación de los sectores de la Tendencia Revolucionaria del Peronismo en el gobierno de Oscar Bidegain en la provincia de Buenos Aires, a partir de las elecciones del 11 de marzo de 1973. Se busca analizar tanto la trayectoria previa de los grupos que integraron la administración provincial, sus lugares específicos en el organigrama gubernamental, junto con las expectativas, los sentidos y los objetivos que guiaron la participación de militantes revolucionarios en las estructuras institucionales del Estado.Por otra parte, se analizan más específicamente las prácticas políticas desplegadas por estos militantes en el Ministerio de Asuntos Agrarios, junto a los programas de gestión impulsados, observando un repertorio heterogéneo de modalidades de acción inscriptas en un programa mayor de cambio de estructuras y construcción del socialismo nacional.

El desafío institucional : Las prácticas políticas no armadas de la Tendencia Revolucionaria del Peronismo en el Ministerio de Asuntos Agrarios de la provincia de Buenos Aires (1973-1974)

Este artículo analiza el proceso de incorporación de los sectores de la Tendencia Revolucionaria del Peronismo en el gobierno de Oscar Bidegain en la provincia de Buenos Aires, a partir de las elecciones del 11 de marzo de 1973. Se busca analizar tanto la trayectoria previa de los grupos que integraron la administración provincial, sus lugares específicos en el organigrama gubernamental, junto con las expectativas, los sentidos y los objetivos que guiaron la participación de militantes revolucionarios en las estructuras institucionales del Estado.Por otra parte, se analizan más específicamente las prácticas políticas desplegadas por estos militantes en el Ministerio de Asuntos Agrarios, junto a los programas de gestión impulsados, observando un repertorio heterogéneo de modalidades de acción inscriptas en un programa mayor de cambio de estructuras y construcción del socialismo nacional

La lógica de la inversión pública en infraestructura en la provincia de Buenos Aires : Entre las necesidades sociales y ¿la política?

El presente estudio se enfoca en la inversión en infraestructura en la provincia de Buenos Aires como un elemento central del cambio estructural pregonado para minimizar los diferenciales de productividad que constituyen la clave de los problemas del ciclo económico argentino. En el enfoque se asume la relación bidireccional entre territorio y escala nacional. Se diferencian para el análisis la infraestructura social, productiva y aquella que es consecuencia de la estructura económica dominante. Se incluye un estudio descriptivo del peso de cada tipo de infraestructura y de la lógica de localización de la inversión, y también se realiza una regresión econométrica mediante la cual se buscan variables explicativas significativas

El desafío institucional : Las prácticas políticas no armadas de la Tendencia Revolucionaria del Peronismo en el Ministerio de Asuntos Agrarios de la provincia de Buenos Aires (1973-1974)

Este artículo analiza el proceso de incorporación de los sectores de la Tendencia Revolucionaria del Peronismo en el gobierno de Oscar Bidegain en la provincia de Buenos Aires, a partir de las elecciones del 11 de marzo de 1973. Se busca analizar tanto la trayectoria previa de los grupos que integraron la administración provincial, sus lugares específicos en el organigrama gubernamental, junto con las expectativas, los sentidos y los objetivos que guiaron la participación de militantes revolucionarios en las estructuras institucionales del Estado.Por otra parte, se analizan más específicamente las prácticas políticas desplegadas por estos militantes en el Ministerio de Asuntos Agrarios, junto a los programas de gestión impulsados, observando un repertorio heterogéneo de modalidades de acción inscriptas en un programa mayor de cambio de estructuras y construcción del socialismo nacional