Conservation of the oligomeric state of native VDAC1 in detergent micelles.

The voltage-dependent anion-selective channel (VDAC) is an intrinsic β-barrel membrane protein located within the mitochondrial outer membrane where it serves as a pore, connecting the mitochondria to the cytosol. The high-resolution structures of both the human and murine VDACs have been resolved by X-ray diffraction and nuclear magnetic resonance spectroscopy (NMR) in 2008. However, the structural data are not completely in line with the findings that were obtained after decades of research on biochemical and functional analysis of VDAC. This discrepancy may be related to the fact that structural biology studies of membrane proteins reveal specific static conformations that may not necessarily represent the physiological state. For example, overexpression of membrane proteins in bacterial inclusion bodies or simply the extraction from the native lipid environment using harsh purification methods (i.e. chaotropic agents) can disturb the physiological conformations and the supramolecular assemblies. To address these potential issues, we have developed a method, allowing rapid one step purification of endogenous VDAC expressed in the native mitochondrial membrane without overexpression of recombinant protein or usage of harsh chaotropic extraction procedures. Using the Saccharomyces cerevisiae isoform 1 of VDAC as a model, this method yields efficient purification, preserving VDAC in a more physiological, native state following extraction from mitochondria. Single particle analysis using transmission electron microscopy (TEM) demonstrated conservation of oligomeric assembly after purification. Maintenance of the native state was evaluated using functional assessment that involves an ATP-binding assay by micro-scale thermophoresis (MST). Using this approach, we were able to determine for the first time the apparent KD for ATP of 1.2 mM.

Dental bibliography ... literature of dental science and art as found in the libraries of the New York academy of medicine and Bernhard Wolf Weinberger,

[pt. I.] A reference index.--pt. II. A subject index, with additional reference index.

Transactions of the Medical Society of the State of New York.

Lists of members in each volume; some of these are merely partial lists, members in attendance at the annual meetings, etc.

Distribution of health services in the structure of State government, 1950.

First ed. published in 1929 by John A. Ferrell under title: Health departments of States and provinces of the United States and Canada.

Serial publications currently received in the New York State Medical Library, Albany, a subject guide.

Mode of access: Internet.

Colorado medicine.

Description based on: Vol. 34, no. 12 (Dec. 1937); title from cover.

Transactions of the State Medical Society of Wisconsin for the year ...

Mode of access: Internet.

The general state of medical and chirurgical practice exhibited : showing them to be inadequate, ineffectual, absurd, and ridiculous ... /

Mode of access: Internet.

Laws of the state of Minnesota relating to the State live stock sanitary board and local boards of health and their duties and powers in dealing with animals and animal diseases. Comp. August 1, 1929.

Mode of access: Internet.

Transactions of the session of the Medical Society of the State of California.

Mode of access: Internet.

Professional education : Medicine including osteopathy and physiotherapy.

Mode of access: Internet.

The Virginia State Veterinary Medical Association.

"Founded 1894."

Veterinary medicine in Pennsylvania: Training facilities and practice;

Report dealing with the feasibility of establishing a school of veterinary medicine at the Pennsylvania State College. Cf: Letter of transmittal, p. v.

[Reports and discussions of meeting held at New York State Veterinary College, Cornell University, July 27, 1963]

Transcript of tape recorded notes.

[Reports and discussions of meeting held at the College of Veterinary Medicine, Michigan University, August 19, 1961]

Transcript of tape recorded notes.