Estudio socio-territorial de la actividad hortícola en el partido de La Plata 1998-2002

El presente trabajo se propone identificar y caracterizar las unidades de análisis espacio-territorial (fracciones censales) de la actividad primaria intensiva, específicamente hortícola, y examinar el peso relativo de la actividad del Partido de La Plata en el contexto provincial y nacional. Se analizan las variables sobre mano de obra, superficie total, superficie cultivada a campo y bajo cubierta, régimen de tenencia de la tierra y componentes tecnológicos. Se examinan y comparan los censos hortícola de la Provincia de Buenos Aires 1998 y Nacional Agropecuario 2002 (CNA02). Los resultados son presentados en tablas, gráficos y mapas temáticos procesados en SIG ArcView.

Estudio socio-territorial de la actividad hortícola en el partido de La Plata 1998-2002

El presente trabajo se propone identificar y caracterizar las unidades de análisis espacio-territorial (fracciones censales) de la actividad primaria intensiva, específicamente hortícola, y examinar el peso relativo de la actividad del Partido de La Plata en el contexto provincial y nacional. Se analizan las variables sobre mano de obra, superficie total, superficie cultivada a campo y bajo cubierta, régimen de tenencia de la tierra y componentes tecnológicos. Se examinan y comparan los censos hortícola de la Provincia de Buenos Aires 1998 y Nacional Agropecuario 2002 (CNA02). Los resultados son presentados en tablas, gráficos y mapas temáticos procesados en SIG ArcView.

Estudio socio-territorial de la actividad hortícola en el partido de La Plata 1998-2002

El presente trabajo se propone identificar y caracterizar las unidades de análisis espacio-territorial (fracciones censales) de la actividad primaria intensiva, específicamente hortícola, y examinar el peso relativo de la actividad del Partido de La Plata en el contexto provincial y nacional. Se analizan las variables sobre mano de obra, superficie total, superficie cultivada a campo y bajo cubierta, régimen de tenencia de la tierra y componentes tecnológicos. Se examinan y comparan los censos hortícola de la Provincia de Buenos Aires 1998 y Nacional Agropecuario 2002 (CNA02). Los resultados son presentados en tablas, gráficos y mapas temáticos procesados en SIG ArcView.

Spatial and seasonal distribution of Thaumarchaeota in the water column and sediment of the southern North Sea

We have examined the spatial and seasonal distribution of Thaumarchaeota in the water column and sediment of the southern North Sea using the specific intact polar lipid (IPL) hexose, phosphohexose (HPH) crenarchaeol, as well as thaumarchaeotal 16S rRNA gene abundances and expression. In the water column, a higher abundance of Thaumarchaeota was observed in the winter season than in the summer, which is in agreement with previous studies, but this was not the case in the sediment where Thaumarchaeota were most abundant in spring and summer. This observation corresponds well with the idea that ammonia availability is a key factor in thaumarchaeotal niche determination. In the surface waters of the southern North Sea, we observed a spatial variability in HPH crenarchaeol, thaumarchaeotal 16S rRNA gene abundance and transcriptional activity that corresponded well with the different water masses present. In bottom waters, a clear differentiation based on water masses was not observed; instead, we suggest that observed differences in thaumarchaeotal abundance with depth may be related to resuspension from the sediment. This could be due to suspension of benthic Thaumarchaeota to the water column or due to delivery of e.g. resuspended sediment or ammonium to the water column, which could be utilized by pelagic Thaumarchaeota. This study has shown that the seasonality of Thaumarchaeota in water and sediment is different and highlights the importance of water masses, currents and sedimentary processes in determining the spatial abundance of Thaumarchaeota in the southern North Sea.

Niche differentiation among mat-forming, sulfide-oxidizing bacteria in chemosynthetic ecosystems of the Nile Deep Sea Fan (Eastern Mediterranean Sea)

Sulfidic muds of cold seeps on the Nile Deep Sea Fan are populated by different types of mat-forming sulfide-oxidizing bacteria. The predominant sulfide oxidizers of three different mats were identified by microscopic and phylogenetic analyses as (i) Arcobacter species producing cotton-ball-like sulfur precipitates, (ii) large filamentous sulfur bacteria including Beggiatoa species, or (iii) single, spherical cells resembling Thiomargarita species. High resolution in situ microprofiles revealed different geochemical settings selecting for different mat types. Arcobacter mats occurred where oxygen and sulfide overlapped at the bottom water interface. Filamentous sulfide oxidizers were associated with non-overlapping, steep gradients of oxygen and sulfide. A dense population of Thiomargarita was favored by temporarily changing supplies of oxygen and sulfide. These results indicate that the decisive factors in selecting for different mat-forming bacteria within one deep-sea province are spatial or temporal variations in energy supply. Furthermore, the occurrence of Arcobacter spp.-related 16S rRNA genes in the sediments below all three types of mats, as well as on top of brine lakes of the Nile Deep Sea Fan, indicates that this group of sulfide oxidizers can switch between different life modes depending on the geobiochemical habitat setting.

Chapter 5 Product Differentiation versus Geographical Differentiation: Evidence from the Pork Processing Industry in China

This chapter attempts to identify whether product differentiation or geographical differentiation is the main source of profit for firms in developing economies by employing a simple idea from the recently developed method of empirical industrial organization. Theoretically, location choice and product choice have been considered as analogues in differentiation, but in the real world, which of these strategies is chosen will result in an immense difference in firm behavior and in the development process of the industry. Development of the technique of empirical industrial organization enabled us to identify market outcomes with endogeneity. A typical case is the market outcome with differentiation, where price or product choice is endogenously determined. Our original survey contains data on market location, differences in product types, and price. The results show that product differentiation rather than geographical differentiation mitigates pressure on price competition, but 70 per cent secures geographical monopoly.

Territory and the governmentalisation of social reproduction: parliamentary enclosure and spatial rationalities in the transition from feudalism to capitalism

Recent applications of Foucauldian categories in geography, spatial history and the history of town planning have opened up interesting new perspectives, with respect to both the evolution of spatial knowledge and the genealogy of territorial techniques and their relation to larger socio-political projects, that would be enriched if combined with other discursive traditions. This article proposes to conceptualise English parliamentary enclosureea favourite episode for Marxist historiography, frequently read in a strictly materialist fashioneas a precedent of a new form of sociospatial governmentality, a political technology that inaugurates a strategic manipulation of territory for social change on the threshold between feudal and capitalist spatial rationalities. I analyse the sociospatial dimensions of parliamentary enclosure’s technical and legal innovations and compare them to the forms of communal self-regulation of land use customs and everyday regionalisations that preceded it. Through a systematic, replicable mechanism of reterritorialisation, enclosure acts normalised spatial regulations, blurred regional differences in the social organisation of agriculture and erased the modes of autonomous social reproduction linked to common land. Their exercise of dispossession of material resources, social capital and community representations is interpreted therefore as an inaugural logic that would pervade the emergent spatial rationality later known as planning.

Territory and the governmentalisation of social reproduction: parliamentary enclosure and spatial rationalities in the transition from feudalism to capitalism

Recent applications of Foucauldian categories in geography, spatial history and the history of town planning have opened up interesting new perspectives, with respect to both the evolution of spatial knowledge and the genealogy of territorial techniques and their relation to larger socio-political projects, that would be enriched if combined with other discursive traditions. This article proposes to conceptualise English parliamentary enclosureea favourite episode for Marxist historiography, frequently read in a strictly materialist fashioneas a precedent of a new form of sociospatial governmentality, a political technology that inaugurates a strategic manipulation of territory for social change on the threshold between feudal and capitalist spatial rationalities. I analyse the sociospatial dimensions of parliamentary enclosure’s technical and legal innovations and compare them to the forms of communal self-regulation of land use customs and everyday regionalisations that preceded it. Through a systematic, replicable mechanism of reterritorialisation, enclosure acts normalised spatial regulations, blurred regional differences in the social organisation of agriculture and erased the modes of autonomous social reproduction linked to common land. Their exercise of dispossession of material resources, social capital and community representations is interpreted therefore as an inaugural logic that would pervade the emergent spatial rationality later known as planning.

Plant β-diversity i in human-altered forest ecosystems: the importance of the structural, spatial, and topographical characteristics of stands in patterning plant species assemblages

An understanding of spatial patterns of plant species diversity and the factors that drive those patterns is critical for the development of appropriate biodiversity management in forest ecosystems. We studied the spatial organization of plants species in human- modified and managed oak forests (primarily, Quercus faginea) in the Central Pre- Pyrenees, Spain. To test whether plant community assemblages varied non-randomly across the spatial scales, we used multiplicative diversity partitioning based on a nested hierarchical design of three increasingly coarser spatial scales (transect, stand, region). To quantify the importance of the structural, spatial, and topographical characteristics of stands in patterning plant species assemblages and identify the determinants of plant diversity patterns, we used canonical ordination. We observed a high contribution of ˟-diversity to total -diversity and found ˟-diversity to be higher and ˞-diversity to be lower than expected by random distributions of individuals at different spatial scales. Results, however, partly depended on the weighting of rare and abundant species. Variables expressing the historical management intensities of the stand such as mean stand age, the abundance of the dominant tree species (Q. faginea), age structure of the stand, and stand size were the main factors that explained the compositional variation in plant communities. The results indicate that (1) the structural, spatial, and topographical characteristics of the forest stands have the greatest effect on diversity patterns, (2) forests in landscapes that have different land use histories are environmentally heterogeneous and, therefore, can experience high levels of compositional differentiation, even at local scales (e.g., within the same stand). Maintaining habitat heterogeneity at multiple spatial scales should be considered in the development of management plans for enhancing plant diversity and related functions in human-altered forests

Efectos territoriales de las denominaciones de origen protegidas de aceite de oliva en España

La implementación de indicaciones geográficas, generan distintos efectos socio-económicos, tecnológicos y ambientales, no sólo a nivel de los productores directamente involucrados, sino también en el marco del sistema agroalimentario local (SIAL). Entre estos últimos, se destaca la organización colectiva de los productores y el desarrollo de una gobernanza, a partir del conjunto de actividades desarrolladas por el Consejo Regulador (CR), en torno al control de calidad y la promoción del producto. El concepto de gobernanza territorial implica llevar a cabo procesos de organización colectiva en red en los que tienen lugar procesos de coordinación multi-nivel entre los agentes, las empresas y las instituciones locales, en un contexto de asimetría en la información y de la existencia de numerosos decisores. El objetivo de esta Tesis Doctoral es realizar un aporte metodológico para el estudio de la gobernanza territorial en sistemas agroalimentarios localizados, a partir de la construcción de indicadores (de calidad, innovación, efectos económicos y prácticas ambientales) y el análisis de las redes de colaboración en materia de difusión del conocimiento e innovaciones técnicas, organizativas y comerciales en la Comarca de Sierra Mágina. Para responder el objetivo se aplica análisis de redes sociales y se construyen indicadores sintéticos para valorizar los efectos. Se realizan encuestas a la totalidad de almazaras presentes en la Comarca de Sierra Mágina, tanto a las que poseen el signo de calidad como a las que no cuentan con dicha marca territorial y entrevistas a testigos privilegiados. En las mismas se consulta sobre las actividades y proyectos que realizan en forma conjunta con otras almazaras e instituciones, así como los actores o referentes a quiénes consultan en temas relacionados con la calidad en la producción olivícola y la obtención de aceite, así como aquellos actores a quienes consultan en aspectos referidos a la Comercialización y gestión. A partir de la información recabada, se obtienen cuatro indicadores sintéticos de efectos de la implantación de una DOP en las almazaras adheridas, referidos a la adopción de innovaciones productivas y organizativas, a la calidad en producto y procesos, a las rentas de diferenciación y a las prácticas ambientales. Asimismo se generan las matrices bi-narias y valuadas para las redes de colaboración en la difusión del conocimiento y se calculan los indicadores particulares. Esto nos permite estudiar las características de las redes que se generan (tamaño, conectividad), el papel del CR en la gobernanza territorial y de otras instituciones del medio, así como identificar actores centrales y grupos en el proceso de difusión de conocimientos. Los resultados muestran que los SIAL pueden beneficiarse de la organización interprofesional y de cooperación interinstitucional a escala local que se producen tras la implantación de una DOP. Se desarrolla un proceso de articulación y un fuerte intercambio de conocimientos entre actores ligados al propio proceso de control de la calidad diferencial. Pero, junto con ello, se desarrollan actividades de formación y de promoción del patrimonio comarcal y se consolidan redes locales de innovación y colaboración mutua en distintas actividades, tanto entre actores de la propia cadena de producción, como entre éstos y las instituciones del medio. Estas actividades, junto con la presencia de un entramado institucional denso, permiten el desarrollo de una gobernanza territorial alrededor del sistema agroalimentario del aceite de oliva. ABSTRACT The implementation of geographical indications has different socio-economic, technological and environmental effects not only for the producers involved but also for the local agro food system (LAFS). Among the latter, we can highlight the collective organization of producers and the development of territorial governance through a series of tasks developed by the Regulatory Board (RB) as regards quality control and product marketing. The concept of territorial governance involves processes of collective organization in networks, where processes of multi-level coordination among agents, enterprises and local institutions take place within a context of asymmetry of information and numerous decision makers. The objective of this Doctoral Thesis is to contribute with methodological tools for the study of territorial governance in local agro food systems by constructing indicators (of quality, innovation, economic effects and environmental practices) and by analyzing networks of collaboration in terms of dissemination of technical, organizational and commercial innovation and knowledge in Comarca de Sierra Mágina. To achieve that objective, social network analysis is applied and synthetic indicators are elaborated to valorize the effects. Interviews to key actors and surveys to every oil mill in Comarca de Sierra Mágina are carried out, not only in those oil mills that host a label of quality but also in the ones that do not hold it. These interviews inquire about tasks and projects performed together with other oil mills and institutions and about actors or referents consulted in topics related to quality in olive production and oil extraction as well as in management and marketing. From all the information collected, four synthetic indicators of the effects of establishing Protected Designations of Origen (PDOs) in the associate oil mills are obtained, in terms of adoption of productive and organizational innovations, process and product quality, income differentiation and environmental practices. In the same way, binary and valorized matrixes are generated for those networks collaborating in the dissemination of knowledge and specific indicators are calculated. This allows to study networks (size, connectivity), the role of RB in territorial governance and other institutions involved and to identify main actors and groups in the process of knowledge dissemination. The results show that the LAFS can benefit from inter-professional organizations and inter- institutional cooperation at local levels by implementing PDOs. A process of articulation and strong exchange of knowledge is developed among those actors involved in controlling differential quality. But, at the same time, activities for the formation and promotion of the “comarca” patrimony are developed and local networks of innovation and mutual collaboration are built for different activities, not only among actors in the production process itself, but between these actors and local institutions as well. These tasks, together with the existence of a dense spatial entrepreneurial network, allow the development of territorial governance on the olive oil agro food system.

A rice homeobox gene, OSH1, is expressed before organ differentiation in a specific region during early embryogenesis.

Homeobox genes encode a large family of homeodomain proteins that play a key role in the pattern formation of animal embryos. By analogy, homeobox genes in plants are thought to mediate important processes in their embryogenesis, but there is very little evidence to support this notion. Here we described the temporal and spatial expression patterns of a rice homeobox gene, OSH1, during rice embryogenesis. In situ hybridization analysis revealed that in the wild-type embryo, OSH1 was first expressed at the globular stage, much earlier than organogenesis started, in a ventral region where shoot apical meristem and epiblast would later develop. This localized expression of OSH1 indicates that the cellular differentiation has already occurred at this stage. At later stages after organogenesis had initiated, OSH1 expression was observed in shoot apical meristem [except in the L1 (tunica) layer], epiblast, radicle, and their intervening tissues in descending strength of expression level with embryonic maturation. We also performed in situ hybridization analysis with a rice organless embryo mutant, orl1, that develops no embryonic organs. In the orl1 embryo, the expression pattern of OSH1 was the same as that in the wild-type embryo in spite of the lack of embryonic organs. This shows that OSH1 is not directly associated with organ differentiation, but may be related to a regulatory process before or independent of the organ determination. The results described here strongly suggest that, like animal homeobox genes, OSH1 plays an important role in regionalization of cell identity during early embryogenesis.

A 20-nucleotide element in the intestinal fatty acid binding protein gene modulates its cell lineage-specific, differentiation-dependent, and cephalocaudal patterns of expression in transgenic mice.

A sequence of epithelial cell proliferation, allocation to four principal lineages, migration-associated differentiation, and cell loss occurs along the crypt-villus axis of the mouse intestine. The sequence is completed in a few days and is recapitulated throughout the life-span of the animal. We have used an intestine-specific fatty acid binding protein gene, Fabpi, as a model for studying regulation of gene expression in this unique developmental system. Promoter mapping studies in transgenic mice identified a 20-bp cis-acting element (5'-AGGTGGAAGCCATCACACTT-3') that binds small intestinal nuclear proteins and participates in the control of Fabpi's cephalocaudal, differentiation-dependent, and cell lineage-specific patterns of expression. Immunocytochemical studies using confocal and electron microscopy indicate that it does so by acting as a suppressor of gene expression in the distal small intestine/colon, as a suppressor of gene activation in proliferating and nonproliferating cells located in the crypts of Lieberkühn, and as a suppressor of expression in the growth factor and defensin-producing Paneth cell lineage. The 20-bp domain has no obvious sequence similarities to known transcription factor binding sites. The three functions modulated by this compact element represent the types of functions required to establish and maintain the intestine's remarkably complex spatial patterns of gene expression. The transgenes described in this report also appear to be useful in characterizing the crypt's stem cell hierarchy.

Knock-out of Arabidopsis metal transporter gene IRT1 results in iron deficiency accompanied by cell differentiation defects

IRT1 and IRT2 are members of the Arabidopsis ZIP metal transporter family that are specifically induced by iron deprivation in roots and act as heterologous suppressors of yeast mutations inhibiting iron and zinc uptake. Although IRT1 and IRT2 are thought to perform redundant functions as root-specific metal transporters, insertional inactivation of the IRT1 gene alone results in typical symptoms of iron deficiency causing severe leaf chlorosis and lethality in soil. The irt1 mutation is characterized by specific developmental defects, including a drastic reduction of chloroplast thylakoid stacking into grana and lack of palisade parenchyma differentiation in leaves, reduced number of vascular bundles in stems, and irregular patterns of enlarged endodermal and cortex cells in roots. Pulse labeling with 59Fe through the root system shows that the irt1 mutation reduces iron accumulation in the shoots. Short-term labeling with 65Zn reveals no alteration in spatial distribution of zinc, but indicates a lower level of zinc accumulation. In comparison to wild-type, the irt1 mutant responds to iron and zinc deprivation by altered expression of certain zinc and iron transporter genes, which results in the activation of ZIP1 in shoots, reduction of ZIP2 transcript levels in roots, and enhanced expression of IRT2 in roots. These data support the conclusion that IRT1 is an essential metal transporter required for proper development and regulation of iron and zinc homeostasis in Arabidopsis.

Spatial and temporal characteristics of summer precipitation over Central Europe in a suite of high-resolution climate models

High-impact, localized intense rainfall episodes represent a major socio-economic problem for societies worldwide, and at the same time these events are notoriously difficult to simulate properly in climate models. Here, the authors investigate how horizontal resolution and model formulation influence this issue by applying the HARMONIE regional climate model (HCLIM) with three different setups; two using convection parameterization at 15 and 6.25 km horizontal resolution (the latter within the “grey-zone” scale), with lateral boundary conditions provided by ERA-Interim reanalysis and integrated over a pan-European domain, and one with explicit convection at 2 km resolution (HCLIM2) over the Alpine region driven by the 15 km model. Seven summer seasons were sampled and validated against two high-resolution observational data sets. All HCLIM versions underestimate the number of dry days and hours by 20-40%, and overestimate precipitation over the Alpine ridge. Also, only modest added value were found of “grey-zone” resolution. However, the single most important outcome is the substantial added value in HCLIM2 compared to the coarser model versions at sub-daily time scales. It better captures the local-to-regional spatial patterns of precipitation reflecting a more realistic representation of the local and meso-scale dynamics. Further, the duration and spatial frequency of precipitation events, as well as extremes, are closer to observations. These characteristics are key ingredients in heavy rainfall events and associated flash floods, and the outstanding results using HCLIM in convection-permitting setting are convincing and encourage further use of the model to study changes in such events in changing climates.

Temporal and spatial transcriptional programs in murine kidney development

We have performed a systematic temporal and spatial expression profiling of the developing mouse kidney using Compugen long-oligonucleotide microarrays. The activity of 18,000 genes was monitored at 24-h intervals from 10.5-day-postcoitum (dpc) metanephric mesenchyme (MM) through to neonatal kidney, and a cohort of 3,600 dynamically expressed genes was identified. Early metanephric development was further surveyed by directly comparing RNA from 10.5 vs. 11.5 vs. 13.5dpc kidneys. These data showed high concordance with the previously published dynamic profile of rat kidney development (Stuart RO, Bush KT, and Nigam SK. Proc Natl Acad Sci USA 98: 5649-5654, 2001) and our own temporal data. Cluster analyses were used to identify gene ontological terms, functional annotations, and pathways associated with temporal expression profiles. Genetic network analysis was also used to identify biological networks that have maximal transcriptional activity during early metanephric development, highlighting the involvement of proliferation and differentiation. Differential gene expression was validated using whole mount and section in situ hybridization of staged embryonic kidneys. Two spatial profiling experiments were also undertaken. MM (10.5dpc) was compared with adjacent intermediate mesenchyme to further define metanephric commitment. To define the genes involved in branching and in the induction of nephrogenesis, expression profiling was performed on ureteric bud (GFP+) FACS sorted from HoxB7-GFP transgenic mice at 15.5dpc vs. the GFP- mesenchymal derivatives. Comparisons between temporal and spatial data enhanced the ability to predict function for genes and networks. This study provides the most comprehensive temporal and spatial survey of kidney development to date, and the compilation of these transcriptional surveys provides important insights into metanephric development that can now be functionally tested.