THE HERPESVIRUS NUCLEAR EGRESS COMPLEX COMPONENT, UL31, IS RECRUITED TO SITES OF DNA DAMAGE THROUGH POLY(ADP-RIBOSE) BINDING

The herpes simplex virus (HSV) UL31 gene encodes a conserved member of the herpesvirus nuclear egress complex that not only functions in the egress of DNA-containing capsids from the nucleus, but is also required for optimal viral genome expression, replication and packaging into capsids. Here, we report that the UL31 protein from HSV-2 and the orthologous protein, ORF69, from Kaposi's sarcoma-associated herpesvirus (KSHV) are recruited to sites of DNA damage. Recruitment of UL31 to sites of DNA damage occurred in HSV-2 infected cells, but did not require other viral proteins. The N-terminus of UL31 contains sequences resembling a poly(ADP-ribose) (PAR) binding motif. As protein poly-ADP ribosylation (PARylation) is a hallmark of the DNA damage response we examined the relationship between PARylation and UL31 recruitment to DNA damage. While the PAR polymerase (PARP)1/2 inhibitor, olaparib, prevented UL31 recruitment to damaged DNA, KU55933 inhibition of signaling through the ataxia telangiectasia mutated (ATM) DNA damage response pathway had no effect. These findings were further supported by experiments demonstrating direct and specific interaction between HSV-2 UL31 and PAR using purified components. Co-transfection with the viral kinase Us3, known to phosphorylate UL31, inhibited UL31 recruitment to DNA damage but also prevented the recruitment of other proteins recruited to DNA damage sites. The viral E3 ubiquitin ligase ICP0 was observed to co-localize with UL31 in transfected cells in a manner that is independent of the PAR-binding ability of UL31. However, inhibition of PARP1/2/3 did not reduce the ability of HSV-2 to replicate and we observed reduced PAR levels in the nuclei of infected cells. This study reveals a previously unrecognized function for UL31 orthologs and may suggest that the recognition of PAR by UL31 is coupled to the nuclear egress of herpesvirus capsids, influences viral DNA replication and packaging, or possibly modulates the DNA damage response mounted by virally infected cells.

Public narratives and the construction of memory among European Muslims

This chapter draws on group and individual interviews with 735 European Muslims in 5 European countries and explores some key aspects of the politics of memory that form an inextricable component of European Muslim self-definitions, discourses and narratives deployed in the attempt to negotiate their inclusion in European societies.

Self-assessments of memory correlate with neuroticism and conscientiousness, not memory span performance

Self-report measures of cognitive problems may have value, but there are indications that scores on such measures are influenced by other factors such as personality. In an online correlational study, 523 non-clinical volunteers completed measures of personality, digit span, and the Prospective and Retrospective Memory Questionnaire. Self-reported prospective and retrospective memory failures were associated positively with neuroticism and negatively with conscientiousness, but not with digit span performance. These findings are consistent with other indications that conscientiousness and neuroticism may underpin self-reports of cognitive problems.

Sites of Excavation and Construction

For his first solo show in Belgium, British artist Neal White presents a range of recent and new work – some of them in collaboration with artist Tina O’Connell – that reflect his ongoing preoccupations with deep time and the spaces of art. OBJECTIF EXHIBITIONS is a not-for-profit institution devoted to the presentation of contemporary art, supported by the Flemish Community. Curated by Antony Hudek

Constructive Synthesis of Memory-Intensive Accelerators for FPGA From Nested Loop Kernels

Field-programmable gate arrays are ideal hosts to custom accelerators for signal, image, and data processing but de- mand manual register transfer level design if high performance and low cost are desired. High-level synthesis reduces this design burden but requires manual design of complex on-chip and off-chip memory architectures, a major limitation in applications such as video processing. This paper presents an approach to resolve this shortcoming. A constructive process is described that can derive such accelerators, including on- and off-chip memory storage from a C description such that a user-defined throughput constraint is met. By employing a novel statement-oriented approach, dataflow intermediate models are derived and used to support simple ap- proaches for on-/off-chip buffer partitioning, derivation of custom on-chip memory hierarchies and architecture transformation to ensure user-defined throughput constraints are met with minimum cost. When applied to accelerators for full search motion estima- tion, matrix multiplication, Sobel edge detection, and fast Fourier transform, it is shown how real-time performance up to an order of magnitude in advance of existing commercial HLS tools is enabled whilst including all requisite memory infrastructure. Further, op- timizations are presented that reduce the on-chip buffer capacity and physical resource cost by up to 96% and 75%, respectively, whilst maintaining real-time performance.

Seasonal variation of n-alkanes and polycyclic aromatic hydrocarbon concentrations in PMsub(10) samples collected at urban sites of São Paulo state, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

TIMESCAPES: an architectural process of memory and adaptation

What if the architectural process of making could incorporate time? All designers who impact the physical environment- consciously and unconsciously are gatekeepers of the past, commentators of the present, and speculators of the future. This project proposes the creation of architecture and adaptive public space that looks to historical memories, foster present day cultural formation, and new alternative visions for the city of the future. The thesis asks what it means to design for stasis and change in a variety of scales- urban, architectural, and detail and arrives at a speculated new neighborhood, institutional buildings, and landscape. Central to this project is the idea of the architect as archeologist, anthropologist, and artist. The project focuses on a rapidly changing part of the city of Fort Worth, Texas and assigns a multipurpose institutional buildings and public space as a method of investigation. The thesis hopes to further architectural discourse about into the role of architecture in the preservation of memory, adaptive potential of public spaces, and the role of time in architecture.

Investigations of the present factors affecting the population characteristics and relative abundance of nile perch (Lates niloticus) at selected trawling sites of western part of Lake Victoria, Uganda, Kagegi Gulf

This report is based on the investigation of the factors affecting population characteristics and relative abundance of Nile perch in selected sites of Kagegi Gulf Lake Victoria Uganda in the month of November 2006. Nine (9) stations were sampled at depth strata of 0-10, 10-20, 20-30 and 30-40 m the mean catch rates were as follows; 8.75±5.5, 4.77±2.3, 6.33±0.3 and 1.34±1.1 tonnes per square kilometer respectively. The catch rates differed at various depth levels with p-value of 0.2940 at 5% level of significance. Limnological parameters were temperature 25.15±0.28, 23.68±0.20, 24.74±0.13 and 25.3±0.20°C; pH of 8.0±0.00, 7.7±0.11; 7.66±0.33 and 6.32±0.14, dissolved oxygen 7.37±0.24, 6.44±0.30, 6.32±0.14 and 6.22±0.14 mg/l; Total nitrogen 589.82±97.2, 514.34±68.8, 690.44±257.8 and 809.03±45.02 µgL-respectively with a p-value of 0.4392 at 5% level of significance. Prey type of Nile perch indicated 65.2% of haplochromine in 0-10 m depth and other strata >10 metre were dominated by Caridina nilotica. Generally investigations indicated that the catch rates of Nile perch at Kagegi gulf in various depth strata probably depended on both the physical and chemical parameters mentioned above.

Ecotoxicological assessment of sediments from the Port of Santos and the disposal sites of dredged material

A dragagem dos sedimentos do Canal de Santos é necessária para permitir o trânsito de navios que operam no Porto de Santos. As áreas de disposição do material dragado estão situadas na zona costeira, em frente à Baía de Santos. Este estudo visou avaliar a qualidade dos sedimentos do Canal de Santos e das áreas de disposição atuais e antigas, utilizando testes de toxicidade de sedimento integral com anfípodos e de toxicidade de elutriatos com embriões de ouriço do mar. As amostras do Canal de Santos foram consideradas as mais tóxicas: todas as amostras dessa área foram consideradas significativamente tóxicas. Além disso, algumas amostras das áreas de disposição exibiram toxicidade. Os resultados mostraram, portanto, que os sedimentos apresentam evidências de degradação em sua qualidade, porém novos estudos devem ser conduzidos visando determinar as relações entre contaminação e toxicidade. Os resultados sugerem ainda que a disposição dos sedimentos dragados deva ser reavaliada.

''Jose Martí: Images of Memory and Mourning", Book Presentation by Emilio Bejel

This flyer promotes the event ''Jose Martí: Images of Memory and Mourning", Book Presentation by Emilio Bejel" cosponsored by the Department of Modern Languages at FIU. This event was held at Books & Books in Coral Gables.

The Herpesvirus Nuclear Egress Complex Component, UL31, is Recruited to Sites of DNA Damage Through Poly(ADP-RIBOSE) Binding

Thesis (Master, Biomedical & Molecular Sciences) -- Queen's University, 2016-08-23 15:03:30.807

Kansallista menneisyyttä rakentamassa ja tulkitsemassa

Suomalaiset arkistoinstituutiot vallan verkostoina ja muistin paikkoina (Making and Interpreting National Pasts - Role of Finnish Archives as Networks of Power and Sites of Memory).

Lugares de Memória da Escravatura e do Tráfico Negreiro (Angola, Cabo Verde, Guiné-Bissau, Moçambique, São Tomé e Príncipe)

Throughout the lengthy duration of this extremely violent slavery phenomenon where man's cruelty is made evident, it gave rise to places of memory: monuments, toponyms, ethnonymns, stories, legends and myths. Collective memory has constantly recycled this basic fabric. The aim of this Catalogue, dedicated to acknowledging the memory of places in the Portuguese-speaking African countries, is to identify, list, map and give information about the different sites of memory: those that can be seen and touched, without however forgetting those which have re-enacted the creative process thanks to oral tradition.

A história e a memória a nos guiar no Rio de Janeiro: proposta para um guia de viagem

A presente dissertação propõe-se a trazer algumas reflexões sobre o turismo cultural no Rio de Janeiro através da criação de um guia de viagem com rotas temáticas baseadas na história da cidade. Este guia proporia novas formas de leitura da paisagem urbana carioca a partir do papel central que a cidade teve na história brasileira enquanto capital política e cultural da nação. O foco principal dos itinerários seria a fase republicana no Brasil entre o final do século XIX até os anos 1970. Nesse período, o Rio passou por uma série de reformas urbanas que seriam também analisadas através de uma série de caminhadas temáticas descritas no guia. A partir dessa abordagem, o trabalho analisa as possibilidades de formatação desse produto turístico através da interlocução entre referenciais teóricos de Memória Coletiva, Lugar de Memória, Identidade Cultural e Interpretação de Patrimônio. Tirando proveito desses conceitos, o estudo procura justificar esse estilo emergente de turismo cultural como um incentivo para o morador do Rio tornar-se viajante em sua própria cidade, enquanto turista-cidadão.

La monumentalización de la ciudad : los sitios de memoria como espacios de intervención experta de los hacedores de ciudad

Este trabajo coloca su foco de atención en la intervención de arquitectos, planificadores, urbanistas y ‘hacedores de ciudad’ en la creación de espacios de memoria sobre la última dictadura militar en la Argentina. A través del análisis de la creación del Parque de la Memoria en la Ciudad de Buenos Aires se mostrará cómo estos espacios se insertan en programas de reforma del espacio urbano que involucran desde dirigentes políticos en campaña electoral hasta la participación de expertos y especialistas en la gestión de la ciudad y cómo la intervención de este universo de agentes – y sus propias representaciones y lógicas de actuación – van a incidir en la manera en que estos espacios de memoria son concebidos, gestados e implementados. Mostraré cómo, dentro de este mundo de relaciones, la memoria y el olvido se vuelven categorías significativas en el marco de sus propias disputas por definir qué es la ciudad, quiénes pertenecen a ella y quiénes son los actores legítimos para intervenir en su definición. A través de esta intervención urbana - la creación del Parque de la Memoria - un área marginal de la ciudad ha sido convertida en un espacio sagrado para conmemorar a las víctimas del Terrorismo de Estado y en un paseo público donde los vecinos de la ciudad realizan diversas actividades recreativas.