Utilização de quitosana obtida de resíduos de camarão para avaliar a capacidade de adsorção de íons Fe3+

A quitosana é produzida através de uma desacetilação alcalina da quitina, a qual é encontrada em exoesqueleto de crustáceos, parede celular de fungos e materiais biológicos. Calcula-se que os resíduos de camarão apresentam de 5 a 7% do seu peso total na forma de quitina, sugerindo que estes sejam utilizados para obtenção do biopolímero. Os processos para obtenção destes biopolímeros consiste nas seguintes etapas: desmineralização, desproteinização e desodorização, obtendo-se assim, a quitina úmida. Após seca, passa por uma desacetilação química para a conversão em quitosana úmida, sendo purificada e posteriormente seca. A quitosana, por apresentar grupamentos amino livres em sua estrutura, é uma molécula capaz de formar complexos estáveis com cátions metálicos. O objetivo geral deste trabalho foi obter quitina a partir de resíduos de camarão (Penaeus brasiliensis) com posterior produção de quitosana, e avaliar sua capacidade de complexação com íons Fe3+, em solução. A quitosana produzida foi caracterizada através do grau de desacetiliação e da massa molecular viscosimétrica, Para caracterização estrutural das amostras de quitosana, utilizaram-se espectrometria de infravermelho e espectrofotometria UV-Visível, bem como para o complexo formado de quitosana e ferro. Para analisar a eficiência da remoção deste íon, foram feitas análises em espectrometria de absorção atômica em chama e em espectrofotometria UV-Visível. Uma análise estatística foi realizada para avaliar a percentagem de remoção do íon ferro das soluções, sendo utilizado um planejamento fatorial em dois níveis, tendo como variáveis independentes o pH do meio, a quantidade de quitosana adicionada, a granulometria da mesma e o tempo de reação. A quitosana apresentou grau de desacetilação de 87±2% e massa molecular viscosimétrica de 196±4kDa, sendo esses valores, comparáveis à quitosana disponível comercialmente. Na melhor região de trabalho definida pela análise estatística, obteve-se uma remoção máxima de 85 % do íon ferro das soluções.

Quantifying gregarine infestation of Penaeus vannamei on a commercial shrimp farm and some attempts at treatment

The gregarine (Nematopsis spp.) infestation in Penaeus vannamei on a commercial shrimp pond is discussed focusing on quantifying the parasites and some attempts to control infestation.

Survey of food supplementary (Pseudomonas fluorescence) and live food (Algae and brine shrimp) on Penaeus indicus larval stages

In this study ,the effects of Pseudomonas fluorescence obtained from generator pond water of Kolahi as supplementary and four algae consisting of : Chaetoceros sp, Chlorella and Skeletonema sp and Tetraselmis sp, three types of artemia as live food larval states from zoa to postlarvae (PL4 ) Penaeus indicus were investigated. The results indicate that Pseudomonas fluorescence has positive effect on Penaeus indicits larvae growth and their living food. Effective ranges at minimum and maximum were estimated. In most cases optimum dosage was approximately determined. Optimum dosage is between 50 -150 milligrams per liter for living food and Penaeus larval More than 200 milligram per liter resulted in a negative effect on the growth and survival. Also the results indicate Uromiana artemia. Requires a higher concentration of the bacteria the imported artemia. As a conclusion it is recommended to introduce Pseudonionas fluorescence as a new medium for the growth of some mentioned algae .

Reo-Like Virus in White Shrimp Penaeus vannamei (Crustacea: Decapoda): Co-occurrence with Baculovirus penaei in Experimental Infections

A virus, tentatively identified as reo-like, occurred concurrently with experimentally-induced Baculovirus penaei (BP) infection in cultured white shrimp larvae Penaeus vannamei. Each shrimp with a reo-like viral infection also had a BP infection, but not all BP-infected shrimp had a reo-like infection. Both viruses occurred in the same tissues and occasionally withln the same cell. The reolike virus developed in epithelial cells of the anterior midgut and in reserve- and fibrillar-cells of the hepatopancreas. The paraspherical and non-enveloped reo-like virions (ca. 50 nm diam.) occurred as unordered aggregates in the cell cytoplasm. Their etiology has not been determined. Reo-like virions may have been introduced along with the BP virus, or, were latent and only manifested due to stress induced by the more pathogenic BP virus.

Paraophioidina scolecoides n. sp., a New Aseptate Gregarine from Cultured Pacific White Shrimp Penaeus vannamei

The aseptate gregarine Paraophloidina scolecoides n. sp. (Eugregarinorida: Lecudinidae) heavily infected the midgut of cultured larval and postlarval specimens of Penaeus vannamei from a commercial 'seed-production' facility in Texas, USA. It is morphologically similar to P. korotneffi and P. vibiliae, but it can be distinguished from them and from other members of the genus by having gamonts associated exclusively by lateral syzygy. Shrimp acquired the infection at the facility; nauplii did not show any evidence of infection, but protozoea, mysis, and postlarval shrimp had a prevalence and intensity of infection ranging from 56 to 80 % and 10 to >50 parasites, respectively. Infected shrimp removed from the facility to aquaria at another location lost their gamont infection within 7 days When voided from the gut, the gregarine disintegrated in seawater. Results suggest that P. vannamei is an accidental host, although a survey of representative members of the invertebrate fauna from the environment associated with the facility failed to discover other hosts. No link was established between infection and either the broodstock or the water or detritus from the nursery or broodstock tanks.

The effects of density on the growth and survival of different families of juvenile Penaeus japonicus Bate

Variations in the growth and survival of six families of juvenile (initial mean weight = 4.16 g) Penaeus japonicus were examined at two densities (48 and 144 individuals m(-2)) in a controlled laboratory experiment. Survival was very high throughout the experiment (95.4%), but differed significantly between densities and rearing tanks. Family, sex and family x density interaction did not significantly affect survival. Mean specific growth rate (SGR) of the shrimp was 18% faster at the low density (1.93 +/- 0.05% day(-1)) than at high density (1.64 +/- 0.03% day(-1)). However, there was a small but significant interaction between family and density indicating that growth of the families was not consistent at both densities. The inconsistent growth of the families across the two densities resulted in a change in the relative performance (ranking) of families at each density. Sex, rearing tank and rearing cage also affected growth of the shrimp. Mean SGR of the females (1.79 +/- 0.03% day(-1)) was 5% faster than males (1.70 +/- 0.03% day(-1)) when averaged across both densities. Shrimp grew significantly faster in rearing tank 3 than rearing tank 1 or 2 at both densities. Results of the present study suggest that family x density interaction could affect the efficiency of selection for growth if shrimp stocks produced from shrimp breeding programs are to be grown across a wide range of densities. Crown Copyright (C) 2004 Published by Elsevier B.V. All rights reserved.

The effects of ionizing radiation on the reproductive capacity of adult Penaeus (Marsupenaeus) japonicus (Bate)

The reproductive capacity of adult Penaeus (Marsupenaeus) japonicus (Bate) was assessed after exposure to ionizing gamma radiation from a cobalt-60 source. Males and females were each exposed to 0, 10 and 20 Gray (Gy) of ionizing radiation (IR) and reciprocally crossed to give nine mating combinations. Fecundity and hatch rate of resulting spawnings were used as measures of reproductive capacity. IR significantly (P

Production of triploid Kuruma shrimp, Marsupenaeus (Penaeus) japonicus (Bate) nauplii through inhibition of polar body I, or polar body I and II extrusion using 6-dimethylaminopurine

This study investigated the chromosome ploidy level of Marsupenaeus (Penaeus) japonicus (Bate) non-viable (unhatched) embryos and nauplii after exposure to 6-dimethylaminopurine (6-DMAP), timed to stop either polar body (PB) I, or PBI and II extrusion. Embryos from eight separate families or spawnings were exposed to 150 or 200 mu M 6-DMAP from 1- to 3-min post-spawning detection (psd) for a 4- to 5-min duration (timed to stop PBI extrusion). Separate aliquots of embryos from five of the same spawnings were also exposed to 200 mu M of 6-DMAP from 1- to 3-min psd for a 16-min duration (timed to stop both PBI and II extrusion). For one spawning, a third aliquot of embryos was exposed to 400 p M of 6-DMAP from 1- to 3-min psd for a 16-min duration (timed to stop both PBI and II extrusion). At 18-h psd, non-viable embryo and nauplii samples were taken separately for fluorescent activated cell sorting (FACS). FACS revealed that there were diploids and triploids among all treated non-viable embryos and nauplii. All control non-viable embryos and nauplii were diploid. Percentages of triploid induction for the 4- to 5-min and 16-min durations were not significantly different (P > 0.05). Additionally, no difference was found in the triploidy level of nonviable embryos compared to nauplii in these treatments. The percentage of triploid embryos and nauplii when exposed to 6-DMAP for a 4- to 5-min duration ranged from 29.57% to 99.23% (average 55.28 +/- 5.45%) and from 5.60% to 98.85% (average 46.70 +/- 7.20%), respectively. The percentage of triploid embryos and nauplii when exposed to 6-DMAP for a 16-min duration ranged from 11.71% to 98.96% (average 52.49 +/- 11.00%) and from 47.5% to 99.24% (average 79.38 +/- 5.24%), respectively. To our knowledge, this is the first documentation of successful PBI or PBI and II inhibition in shrimp. This study conclusively shows that treatment of M. japonicus embryos with 6-DMAP at 1- to 3-min pscl for either a 4- to 5-min duration (timed to stop PBl extrusion) or 16-min duration (timed to stop both PBI and II extrusion) results in viable triploid nauplii. (c) 2006 Elsevier B.V. All rights reserved.

Spawning pattern of the Nematopalaemon hastatus (Crustacea: Palaemonidae) (Aurivilius, 1898), exploited in the artisanal shrimp fishery in the outer Cross River estuary

Some aspects of the reproduction of Nematopalaemon hastatus in the artisanal shrimp fishery in the outer Cross River estuary (Nigeria) was investigated. 2 kg samples were taken from the daily catch of the N. hastatus fishery on a twice-weekly basis. The N. hastatus in the sample were then observed for gravidity, using a hand lens, and their post-orbital carapace length (POCL) taken. This was done over a 12-month period. The eggs in formalin-fixed samples showed colour changes from light orange to dark brown with maturation. Gravid females with eggs in different stages of maturation, from light orange to dark brown were observed in the samples. The reproduction occurs all year-round. The proportion of gravid females observed in samples had two distinct peaks in June and November, and two distinct troughs in May and October. The lowest length at which gravidity was observed was 6.0 mm POCL. There were no Penaeus notialis in the samples

Survey of the shrimp stocks of the Sierra Leone inshore shelf : a summary report outlining objectives, observations and recommendations on the status of the Sierra Leone shrimp fishery

This report gives the species and catch-rates per hour of some commercial shrimp species caught in the course of a short survey of inshore shelf fishing grounds. Species seen in significant quantities were: Parapenneopsis atlantica, Parapenaeus longirostris, Penaeus kerathurus and Penaeus duorarum notialis

Biologie de Penaeus duorarum en Côte d'Ivoire. 3. Étude des variations quotidiennes des rendements et leur relations avec l'hydroclimat. Essai de généralisation au Golfe de Guinée

Hydroclimatic conditions in the Gulf of Guinea between Senegal and Nigeria are briefly described emphasizing the seasonal variations of transparency. Analysis of the Abidjan based shrimp fleet allowed to the description of the seasonal variations of activity rhythms for Côte d'Ivoire, Ghana, and Nigeria. These rhythms are different between seasons, between fishing grounds, and sometimes even between depths on a given ground. These variations follow the turbidity ones. Diurnal activity is observed in very turbid waters, nocturnal and transition activity in clearer ones. The authors assume that the basic behaviour is a nocturnal one, but that the shrimp-trawlers catches reflect some apparently different ones resulting from diel variations in the stock availability. To explain the apparently diurnal behaviour observed most of the year over the whole Gulf of Guinea it is suggested that these generally benthic shrimps become nectonic at night when turbidity is very high. The results obtained in Ivory Coast, Ghana and Nigeria are compared to those from Senegal where hydroclimatic conditions are different. The similarities are emphasized. The differences in observed behaviour are supposedly caused by the cold season water temps which are sufficiently low to disturb the nor mal activity rhythm.

Population parameters of Penaeus californiensis in the Gulf of Guayaquil, Ecuador

The growth, mortality, and recruitment pattern of Penaeus californiensis were investigated using tail length (TL)-frequency data obtained from the Gulf of Guayaquil shrimp population. Computer-based methods of tail-frequency analysis Compleat ELEFAN software were used. Results obtained gave relatively high growth and mortality estimates for both males and females. The recruitment pattern indicated two pulses annually, one significantly larger than the other.

Stocking, Enhancement, and Mariculture of Penaeus orientalis and Other Species in Shanghai and Zhejiang Provinces, China

China's marine aquaculture landings provide only 18% of its combined freshwater and amrine capture and culture landings, at a per-capita consumption of only 3.2 kg/yr out of a total of 18.1 kg/yr. We described development and some of the results of long-term mariculture and stocking/enhancement projects that have been underway for up to 20 years in the Hangzhou Bay area. Penaeus orientalis (also referred to as P. chinensis) stocking provided up to 400 t/yr, at a total cost-benefit ratio of up to 8 Yuan of landed shrimp per Yuan invested in shrimp stocking. Over 40 t of Penaeus orientalis were produced commercially in 1993, with proceeds being used to fund mariculture and fisheries research. Large scale edible jellyfish restocking is also underway, while semicommercial culture of abalone, Haliotis diversicolor, has been successful. Technical problems limitig mariculture have been solved successfully for some species.

The By-catch From the Artisanal Shrimp Trawl Fishery, Gulf of Paria, Trinidad

Samples of shrimp trawl catches were collected from a commercial artisanal vessel fishing inside the 6-fm isobath in the Gulf of Paria, Trinidad. From August 1986 to May 1987, 34 late evening-early morning trawl trips were made and 97 hauls were sampled. Annual ratio estimates were 9 (SD 1.3) finfish:shrimp and 14.7 (SD 2.0) by-catch: shrimp, with the highest ratios observed August through December and the lowest from late January through May, the dry season. Extrapolation of ratios, using shrimp catch statistics, indicates that for 1986, 974,000 kg of finfish and 620,000 kg of crabs, Callinectes spp., were caught incidentally by artisanal shrimp trawlers fishing in the Gulf of Paria. Of this total incidental catch (1,594,000 kg), about 1,500,000 kg were discarded (94 percent). Four penaeid shrimp species are targeted: Penaeus schmitti, P. notialis, P. subtilis, and Xiphopenaeus kroyeri. Callinectes spp. were caught in large quantities from Augustto mid-January. Small (4-15 cm) pelagic and demersal species of little commercial importance dominated the finfish by-catch: Harengula spp., Cetengraulis edentulus, Chloroscombrus chrysurus, Eucinostomous spp., Diapterus rhombeus, and Cyclopsetta spp. Altogether, the monthly percentage of the species ranged from 70 to 85 percent of the total finfish by-catch.

Optimization of stocking density for environmental-friendly improved extensive shrimp farming system in south-west part of Bangladesh

An experimental culture practice of P. monodon on extension approach was conducted in two brackish water earthen ponds of Demonstration Farm and Training Center (DFTC), Kaliganj, Satkhira. The experiment was aimed to provide farmers with appropriate technology that can immediately improve pond yield with keeping the environment in friendly condition. For optimization of stocking density of a cost effective environmental friendly improved extensive shrimp farming, the ponds were stocked with coastal river post larvae of P. monodon at the stocking rates of 2 pls/m² and 2.5 pls/m² without supplementary feeding. To control experimental error another five farmer's gher were used as replicates of each demo-pond. Considering the farmers buying ability, cost of inputs and other facilities kept minimal. The impact of stocking density was evaluated on the basis of growth, survival rate, production and economic return. Better production (average 299.01 kg/ha) with same survival rate (39.33%) were found with a stocking density of 2.5 pls/m² without causing any deterioration in the culture environment.