Movimentos na escrita inicial de crianças: um estudo longitudinal de hipersegmentações

Pós-graduação em Estudos Linguísticos - IBILCE

Comparação dos padrões cinemáticos de chutes realizados por participantes com 15 e 17 anos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Preparação de espuma cerâmica à base de zircônia pelo processo sol-emulsão-gel

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Produtividade de massa seca e proteina bruta do capim-elefante cv. napier em função da adubação orgânica e mineral

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Percorrendo o universo de devaneios, distorções e dualidades: considerações acerca da dramaturgia de Qorpo-Santo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A re-significação da feminilidade de mulheres da terceira idade durante seu processo de envelhecimento

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Para além dos Contos de Fadas: o ideal e o real no pensamento das mulheres sobre o casamento

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O dispositivo intercessor como meio de superação dialética da medicalização da saúde mental

Pós-graduação em Psicologia - FCLAS

Caracterização morfológica, química e conservação in vitro de Pfaffia glomerata(Sprengel)Pedersen

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Cronistas do Rio: o processo de modernização do Rio de Janeiro nas crônicas de Olavo Bilac (Kosmos, 1904-1908) e Lima Barreto (Careta, 1915-1922)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Vidas em enclaves. Imaginário das cidades inseguras e fragmentação socioespacial em contextos não metropolitanos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

GQ e Men's Health: os estilos de vida do ator masculino contemporâneo

Pós-graduação em Linguística e Língua Portuguesa - FCLAR

Measurement of the W gamma and Z gamma inclusive cross sections in pp collisions at root s=7 TeV and limits on anomalous triple gauge boson couplings

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

2 '-Deoxyguanosine, 2 '-deoxycytidine, and 2 '-deoxyadenosine adducts resulting from the reaction of tetrahydrofuran with DNA bases

A recent study showed that tetrahydrofuran (THF), a widely used solvent, is carcinogenic in experimental animals. Despite its carcinogenic activity, there is a paucity of information regarding cellular toxicity, biomolecular damage, and genotoxicity induced by THF. We describe here the structural characterization of adducts produced by the reaction of oxidized THF with 2'-deoxyguanosine (dGuo-THF 1 and dGuo-THF 2), 2'-deoxyadenosine (dAdo-THF), and 2'-deoxycytidine (dCyd-THF). Adducts were isolated from in vitro reactions by reverse-phase HPLC and fully characterized on the basis of spectroscopic measurements. The stable derivatives obtained by the reduction of adducts with NaBH4 ( the case of dGuo-THF 1, dCyd-THF, and dAdo-THF) and the stable adduct dGuo-THF 2 were used as standards for optimization of chromatographic separations for adduct detection in DNA through HPLC/ESI/MSMS. Using this methodology, we successfully detected the four adducts in calf thymus DNA reacted with oxidized THF. The present study also provides evidence that rat liver microsomal monooxigenases oxidize THF to the reactive electrophilic compounds that are able to damage the DNA molecule, as indicated by a significant increase in adduct dGuo-THF 1 level when NADPH was added to the THF/ microsomes/dGuo incubation mixtures. Our data point to DNA-THF adducts as possible contributing factors to the toxicological effects of THF exposure.

Comparative study over methods developed for quantification of darunavir in tablets by environmental friendly infrared and capillary electrophoretic techniques

Darunavir, a protease inhibitor used in the treatment of HIV infection, presents few methods for its determination in pharmaceuticals. Infrared (IR) spectroscopy offers the possibility of obtaining spectra relatively quickly, providing interesting information, analytically, qualitatively or quantitatively. Capillary electrophoresis (CE) performs separations of high efficiency in shorter time with reagents and samples in small quantity. These two methods are cost-benefitted when we evaluate the green level and the cost of analysis. Faster and cheaper methods without generating organic waste by IR and CE for the quantification of darunavir were developed and validated, focusing socioeconomic impact of analytical decisions. If the cost of acquisition, maintenance, production, analysis and conditioning of drugs and pharmaceuticals is high, consequently the price of this product in the market will be higher and it cannot be accessible to the patient. Treatment failure not only affects the quality of life of patients, but also contributes significantly to the economic burden of the health system. In this context there is a tool called Analysis of the Life Cycle, which comes to make us think in a multidimensional way focusing the whole, the parts and especially the interaction among the parts of a system.