The effect of lysobisphosphatidic acid (LBPA) on α-tocopherol transfer protein (α-TTP) binding to lipid membranes

The a-tocopherol transfer protein (a-TTP) is responsible for the retention of the atocopherol form of vitamin E in living organisms. The detailed ligand transfer mechanism by a-TTP is still yet to be fully elucidated. To date, studies show that a-TTP transfers a-tocopherol from late endosomes in liver cells to the plasma membrane where it is repackaged into very low density lipoprotein (VLDL) and released into the circulation. Late endosomes have been shown to contain a lipid known as lysobisphosphatidic acid (LBP A) that is unique to this cellular compartment. LBPA plays a role in intracellular trafficking and controlling membrane curvature. Taking these observations into account plus the fact that certain proteins are recruited to membranes based on membrane curvature, the specific aim of this project was to examine the effect of LBP A on a-TTP binding to lipid membranes. To achieve this objective, dual polarization interferometry (DPI) and a vesicle binding assay were employed. Whilst DPI allows protein binding affinity to be measured on a flat lipid surface, the vesicle binding assay determines protein binding affinity to lipid vesicles mimicking curved membranes. DPI analysis revealed that the amount of a-TTP bound to lipid membranes is higher when LBPA is present. Using the vesicle binding assay, a similar result was seen where a greater amount of protein is bound to large unilamellar vesicles (LUV s) containing LBP A. However, the effect of LBP A was attenuated when small unilamellar vesicles (SUVs) were replaced with LUVs. The outcome of this project suggests that aTTP binding to membranes is influenced by membrane curvature, which in turn is induced by the presence of LBP A.

The Transfer of Wilderness Trips to the Everyday Lives of Young People: A Case Study

There has been considerable research that investigates the outcomes, benefits, and perceptions of wilderness trip experiences. However, the transfer of learning from a wilderness trip to the everyday lives of youth trip participants, that has deliberately explored post-trip experience, is minimal. Using a qualitative multi-case study approach, methods of data collection included interviews with six youth program participants, six parents/legal guardians, and three program staff, as well as document solicitation. Reports from the participants suggested that the key content transferred to a post-wilderness trip context were interpersonal skills; life skills; and instances of personal growth. Participants applied their learning content from the wilderness trip to school; sports; community and international volunteering; work and career aspirations; family and home life; and social life contexts. Implications for adventure programming and curriculum design, instruction, and transfer are considered.

Letter Addressed to Robert D. Band from A.R.W. Jones

Letter addressed to Robert D. Band from A.R.W. Jones of the Personal Trust Department of the Royal Trust Company, Toronto listing the securities to be held by Mr. Band and his sister and brother, Sept. 5, 1961.

Printed Blank Regarding the Insolvent Act of 1864 from James McWhirter to S.D. Woodruff

Printed blank regarding the Insolvent Act of 1864, this was sent to S. D. Woodruff in the matter of William Little, an insolvent. There was to be a meeting on the 10th day of October for public examination of the insolvent. The document was signed by James McWhirter, official assignee, Sept. 20, 1866.

Receipt to S.D. Woodruff from Harnden Co. Stone for charges on the level

Receipt to S.D. Woodruff from Harnden Co. Stone for charges on the level, 1846.

Letter to S.D. Woodruff from Wm. Shelley, treasurer of the Jarvis-Conklin Mortgage Trust Company

Letter (1 page, typed) to S.D. Woodruff from Wm. Shelley, treasurer of the Jarvis-Conklin Mortgage Trust Company requesting the papers for the Crew and Atkins loans. The letter is discoloured along the folds. This does not affect the text, Nov. 20, 1887.

Letter to S.D. Woodruff from Wm. Shelley, treasurer of the Jarvis-Conklin Mortgage Trust Company

Letter (1 page, typed) to S.D. Woodruff from Wm. Shelley, treasurer of the Jarvis-Conklin Mortgage Trust Company requesting the Dennis loan papers, Jan. 6, 1888.

Letter to S.D. Woodruff from Wm. Shelley, treasurer of the Jarvis-Conklin Mortgage Trust Company

Letter to S.D. Woodruff from Wm. Shelley, treasurer of the Jarvis-Conklin Mortgage Trust Company to please look for the Maria Cogswell loan papers, Aug. 14, 1888.

Letter to S.D. Woodruff from Wm. Shelley, treasurer of the Jarvis-Conklin Mortgage Trust Company

Letter to S.D. Woodruff which accompanied to check for the Maria Cogswell account. The letter was sent by Wm. Shelley, treasurer of Jarvis-Conklin Mortgage Trust Company, May 25, 1889.

Letter to S.D. Woodruff from Thomas H. Johnson of the Department of Crown Lands

Letter to S.D. Woodruff from Thomas H. Johnson of the Department of Crown Lands replying to Mr. Woodruff’s inquiry about the boundaries of berth 192, Dec. 8, 1877.

Letter to S.D. Woodruff from J.J.B. at the National Hotel in East Saginaw, Michigan

Letter to S.D. Woodruff from J.J.B. at the National Hotel in East Saginaw, Michigan. The writer informs Mr. Woodruff that there is about 26,000 of first class board pine which was cut. Mr. Woodruff did not give a receipt to Barton and Bro., Jan. 20, 1880.

Letter to S.D. Woodruff from R. J. Thompson

Letter (1 double sided page and a half, handwritten) to S.D. Woodruff from R. J. Thompson who claims that he was not paid what he was owed by Burton. He is now foreman for McArthur Bros. and used to be the foreman for Burton Bros. He says that he will be in St. Catharines and will call and see Mr. Woodruff, Feb. 19, 1880.

Letter to S.D. Woodruff from R.J. Thompson

Letter to S.D. Woodruff from R.J. Thompson saying that he cannot be in St. Catharines because McArthur Bros. is building a saw mill. A. Denver is the only man that he could recommend for the job, May 5, 1880.

Voucher from the Engineer Department of Port Dalhousie and Thorold Railway Extension to R. and H.H. Collier for lumber accompanied by an itemized list, May 11, 1857

Voucher from the Engineer Department of Port Dalhousie and Thorold Railway Extension to R. and H.H. Collier for lumber accompanied by an itemized list, May 11, 1857.

Letter of request to Charles Stuart from S.D. Woodruff for payment for expenses for surveying the macadamized road from Port Robinson to Hurst’s Bridge

Letter of request to Charles Stuart from S.D. Woodruff for payment for expenses for surveying the macadamized road from Port Robinson to Hurst’s Bridge, July 4, 1855.