Strödda tankar. : Ord till ungdomen och dess fostrare.

Mode of access: Internet.

A history of Scandinavian fishes.

Translated by D. Lloyd Morgan.

La vie rustique en Alsace (Territoire de Belfort) avec une préface de Paul Fort.

Mode of access: Internet.

Malavika et Agnimitra, drama indicum Kalidasae adscriptum;

The Latin translation was never published.

Åminnelse-tal öfver Gabriel Lauræus på Kongl. vetenskaps academiens vägnar, hållit den 19. dec., 1755.

Mode of access: Internet.

Spansk-amerikanska kriget /

Consists of a short history of the Spanish-American War by Peterson, a poem by Runeberg historically concerned with the Russian conquest of Finland, and a poem by Tegner based on a Norwegian story.

De splitsing van het dihydro-ortho-phtalzuur.

Thesis (doctoral)--Universiteit van Amsterdam.

The history of the papacy in the XIXth century,

i. Introduction, Pius VII.--II. Leo Xii to Pius IX.

Sammansatta ord i nutida svenskan undersokta med hansyn till bildning av forleder

Mode of access: Internet.

Theoretical petrology

Mode of access: Internet.

Karl XII:s död : en utredning /

Includes bibliographical references.

Ad quadratum; a study of the geometrical bases of classic & medieval religious architecture, with special reference to their application in the restoration of the cathedral of Nidaros (Throndhjem) Norway,

"Rules of religious architecture were a secret science in the middle ages, just as they were in classic times ... Through this secrecy, the rules became forgotten ... It is this forgotten science which we have discovered, and which we develop in this work."--p. xxii.

Exceptional overproduction of a functional human membrane protein

Eukaryotic-especially human-membrane protein overproduction remains a major challenge in biochemistry. Heterologously overproduced and purified proteins provide a starting point for further biochemical, biophysical and structural studies, and the lack of sufficient quantities of functional membrane proteins is frequently a bottleneck hindering this. Here, we report exceptionally high production levels of a correctly folded and crystallisable recombinant human integral membrane protein in its active form; human aquaporin 1 (hAQP1) has been heterologously produced in the membranes of the methylotrophic yeast Pichia pastoris. After solubilisation and a two step purification procedure, at least 90 mg hAQP1 per liter of culture is obtained. Water channel activity of this purified hAQP1 was verified by reconstitution into proteoliposomes and performing stopped-flow vesicle shrinkage measurements. Mass spectrometry confirmed the identity of hAQP1 in crude membrane preparations, and also from purified protein reconstituted into proteoliposomes. Furthermore, crystallisation screens yielded diffraction quality crystals of untagged recombinant hAQP1. This study illustrates the power of the yeast P. pastoris as a host to produce exceptionally high yields of a functionally active, human integral membrane protein for subsequent functional and structural characterization. © 2007 Elsevier Inc. All rights reserved.

Improving recombinant eukaryotic membrane protein yields in Pichia pastoris:the importance of codon optimization and clone selection

In the last 15 years, 80% of all recombinant proteins reported in the literature were produced in the bacterium, Escherichia coli, or the yeast, Pichia pastoris. Nonetheless, developing effective general strategies for producing recombinant eukaryotic membrane proteins in these organisms remains a particular challenge. Using a validated screening procedure together with accurate yield quantitation, we therefore wished to establish the critical steps contributing to high yields of recombinant eukaryotic membrane protein in P. pastoris. Whilst the use of fusion partners to generate chimeric constructs and directed mutagenesis have previously been shown to be effective in bacterial hosts, we conclude that this approach is not transferable to yeast. Rather, codon optimization and the preparation and selection of high-yielding P. pastoris clones are effective strategies for maximizing yields of human aquaporins.