The influence of the region between residues 220 and 344 and beyond in Phrixotrix railroad worm luciferases green and red bioluminescence

To find the regions having a major influence on the bioluminescence spectra of railroad worm luciferases, we constructed new chimeric luciferases switching the fragments from residues 1-219 and from 220-545 between Phrixotrix viviani (PxvGR; λmax = 548 nm) green light-emitting luciferase and Phrixothrix hirtus (PxhRE; λmax = 623 nm) red light-emitting luciferases. The emission spectrum (λmax = 571 nm) and KM for luciferin in the chimera PxRE220GR (1-219, PxhRE; 220-545, PxvGR) suggested that the region above residue 220 of PxvGR had a major effect on the active site. However, switching the sequence between the residues 226-344 from PxvGR luciferase into PxhRE (PxREGRRE) luciferase resulted in red light emission (λmax = 603 nm), indicating that the region 220-344 by itself does not determine the emission spectrum. Furthermore, the sequence before residue 220 of the green-emitting luciferase is incompatible for light emission with the sequence above residue 220 of PxhRE. These results suggest that the fragments before and after residue 220, which correspond to distinct subdomains, may fold differently in the green- and red-emitting luciferases, affecting the active site conformation.

Los comerciantes de productos agrícolas de San Vicente y las Granadinas, Granada, Dominica y Santa Lucía = The agricultural traders of St. Vincent and the Grenadines, Grenada, Dominica and St. Lucia

Incluye Bibliografía

St. Vincent and the Grenadines country paper

Describe la organizacion estructural, los programas de investigacion agricola, el desarrollo de los recursos humanos, las relaciones con organismos internacionales, y la trasmision de informacion y tecnologia del sistema nacional de investigacion agricola de St. Vincent & Grenadines.

St. Vincent and the Grenadines: the yachting sector

Includes bibliography

Women as the recipients of services from resources allocated in the national budget of St. Vincent and the Grenadines

Includes bibliography

Report of Mission on Port Administration in the East Caribbean Countries: St. Vincent, St. Lucia and Dominica: 29 June to 28 September 1971

Includes bibliography

Report on a familiarization tour of Montserrat, St. Kitts-Nevis-Anguilla, Antigua, St. Lucia and Barbados (8-25 March 1977)

Informe sobre el desarrollo industrial en Montserrat, San Cristóbal Nieves y Anguila, Santa Lucía y Barbados. Analiza brevemente algunas industrias específicas e identifica proyectos industriales que podrían desarrollarse en estos países