Bulletin / Washington (State). State University, Pullman. Engineering Experiment Station.

Mode of access: Internet.

Bulletin of the Utah Engineering Experiment Station.

Includes proceedings of several conferences.

Annual Report of the Secretary of the State Board of Agriculture and Annual Report of the Experiment Station

Mode of access: Internet.

Work of the Hawaiian Experiment Station.

Mode of access: Internet.

Experiment station r

Mode of access: Internet.

Bulletin of the Experiment Station of the Hawaiian Sugar Planters' Association.

Mode of access: Internet.

Agricultural Research Report

Mode of access: Internet.

Bulletin of the Utah Engineering Experiment Station.

Numbered also in the university's Bulletin series

Biennial report of the Board of Trustees of the North Dakota Agricultural College to the governor of North Dakota.

Mode of access: Internet.

The integration of selected boll weevil suppression techniques in an eradication experiment /

"In cooperation with Texas Agricultural Experiment Station."

Some viticultural and oenological experiments conducted at the Paarl Viticultural Experiment Station during 1915-1916 /

"No. 21, 1916."

A study of fatigue cracks in car axles : a report of the investigation conducted by the Engineering experiment station, University of Illinois, in coöperation with Utilities coöperative research committee

Part II, by Herbert F. Moore, Stuart W. Lyon and Norville J. Alleman.

A study of fatigue cracks in car axles : a report of the investigation conducted by the Engineering experiment station, University of Illinois, in coöperation with Utilities coöperative research committee

Part II, by Herbert F. Moore, Stuart W. Lyon and Norville J. Alleman.

Evaluation of GFP Tag as a Screening Reporter in Directed Evolution of a Hyperthermophilic beta-Glucosidase

By applying a directed evolution methodology specific enzymatic characteristics can be enhanced, but to select mutants of interest from a large mutant bank, this approach requires high throughput screening and facile selection. To facilitate such primary screening of enhanced clones, an expression system was tested that uses a green fluorescent protein (GFP) tag from Aequorea victoria linked to the enzyme of interest. As GFP`s fluorescence is readily measured, and as there is a 1:1 molar correlation between the target protein and GFP, the concept proposed was to determine whether GFP could facilitate primary screening of error-prone PCR (EPP) clones. For this purpose a thermostable beta-glucosidase (BglA) from Fervidobacterium sp. was used as a model enzyme. A vector expressing the chimeric protein BglA-GFP-6XHis was constructed and the fusion protein purified and characterized. When compared to the native proteins, the components of the fusion displayed modified characteristics, such as enhanced GFP thermostability and a higher BglA optimum temperature. Clones carrying mutant BglA proteins obtained by EPP, were screened based on the BglA/GFP activity ratio. Purified tagged enzymes from selected clones resulted in modified substrate specificity.