Relação entre carga parasitária de cães naturalmente acometidos por leishmaniose visceral e infectividade para Lutzomyia longipalpis: Lilian Aparecida Colebrusco Rodas. -

Pós-graduação em Ciência Animal - FMVA

Epidemiologia molecular de rotavírus em rebanhos bovinos no estado de São Paulo no período de 2006 a 2010

Pós-graduação em Medicina Veterinária - FCAV

Relação entre presença de anticorpos anti-leishmania spp. e carga parasitária em sangue de cães de área endêmica para leishmaniose visceral

Pós-graduação em Ciência Animal - FMVA

Imunofenótipos de linfócitos T no sangue e no liquor de cães com leishmaniose visceral: correlação com as lesões encefálicas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Caracterização das espécies de leishmania em sangue periférico de cães por PCR-RFLP NA área endêmica de Bauru/SP

Pós-graduação em Ciência Animal - FMVA

Infecção de Lutzomyia longipalpis por leishmania spp. em área urbana endêmica para leishmaniose visceral no Estado de São Paulo, Brasil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação das lesões encefálicas e fenotipagem de linfócitos T e células dentríticas em linfócitos de cães com leishmaniose visceral

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Validação da intradermoreação de Montenegro para diagnóstico de leishmaniose em felinos

Pós-graduação em Ciência Animal - FMVA

Avaliação hepática de cães naturalmente infectados por leishmaniose visceral canina: aspectos ultrassonográficos modo B e Doppler

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Epidemiologia molecular do vírus da Hepatite C: análise comparativa de diferentes regiões subgenômicas aplicadas a estudos de associação genética

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Orientação sobre posse responsável em uma área endêmica para leishmaniose visceral canina

The purpose of this work was to provide guidance to householders in the responsible care of their pets, in a region where canine visceral leishmaniasis is endemic. Questionnaires on responsible ownership and the control of this disease were completed by owners of dogs and cats in the city of Araçatuba, São Paulo State, Brazil, in order to evaluate their level of knowledge. Based on the identified deficiencies, the project team provided the owners of the pets with individual guidance at home, focusing on the most critical issues. Concerning nutrition, 70.45% (62/88) of owners provided pet food only, 27.28% (24/88) administered a mixed diet, and 2.27% (2/88) provided homemade food. Additionally, 78.41% (69/88) did not permit the consumption of raw meat, rare meat, or bones. 9.09% (8/88) of owners allowed their pets to wander the neighborhood alone. Among dogs, 14.77% (13/88) had never been clinically tested by a qualified professional, and 6.82% (6/88) and 15.91% (14/88) had never received any kind of vaccine or vermifuge, respectively. In relation to population control, only 20.45% (18/88) of the dogs had been sterilized. It was also observed that 25% (22/88) of owners had already had canine visceral leishmaniasis seropositive dogs in their homes. 54.55% (12/22) of these owners had referred their pets to veterinary clinics for euthanasia, 22.73% (5/22) had used the services of the city’s animal disease control center, and 18.18% (4/22) had paid for private treatment. Nevertheless, 35.23% (31/88) of the dogs had never been submitted to tests for infection diagnosis. From the results obtained, it could be inferred that the individuals who participated in this study were poorly informed concerning basic pet care, demonstrating a need to implement community awareness campaigns for the responsible ownership of pets.

Avaliação do grau de conhecimento sobre Leishmaniose e Toxoplasmose em moradores do município de Araçatuba, SP

Considering the importance of zoonotic infections by Leishmania spp. and Toxoplasma gondii in Araçatuba region, São Paulo, this study determined the degree of knowledge of residents of Araçatuba about these two diseases. Questionnaires about these diseases were applied to 123 people. Regarding schooling, all the respondents were literate, and 69.9% (86/123) did not attend higher education and 30.8% (37/123) completed an undergraduate degree. Of these, 91.9% (34/37) knew the meaning of the term zoonosis, with significant difference compared to those who were not trained. This word was unknown by 57% (29/123) of total respondents. Most non-graduates, 59.3% (51/86) did not know what is toxoplasmosis and among graduates, 35.1% (13/37) ignored the subject.There were significant difference between these groups. Regarding the prevention of Leishmania infection, a greater proportion of the residents advocated environmental cleanup, the collar repellent and use of citronella. In relation to toxoplasmosis, 54.5% did not know the ways of transmission of this disease and among those with graduate degree, 13 ignoring any transmission medium. When it comes to prevention of this disease, 55.3% (68/123) did not know any prophylactic. In the group of higher education, 14 did not know how to prevent toxoplasmosis. The results of this study show that the majority of respondents are not aware about the mode of transmission of these diseases, especially in relation to toxoplasmosis.

Detecção de co-infecções por Leishmania (l.) chagasi, Trypanosoma evansi, Toxoplasma gondii e Neospora caninum em cães

Co-infections by Leishmania (L.) chagasi, Trypanosoma evansi, Toxoplasma gondii and Neospora caninum in dogs were investigated. Amastigotes forms of Leishmania spp. were detected by cytopathological analysis of lymph nodes in 46,42% (39/84) of dogs. In a male dog, adult, without defined breed, from rural area and positive for Leishmania, were observed flagellated forms of T. evansi in blood smear. By immunofluorescence antibody test, 5,95% (5/84) of dogs were considered reactive to T. gondii, with titer equal to or higher than 1:64, while 3,57% (3/84) were reactive to N. caninum, with titer ≥1:50. Among the animals with visceral leishmaniasis, one showed positive serological response to T. gondii and two for N. caninum. All dogs reactive to N. caninum were from rural area and the predominance of infection by T. gondii was in dogs from urban area. A young male dog from the rural area and seropositive for T. gondii showed Ehrlichia spp. morulae in the cytology and positive reaction for canine distemper virus. Thus, further studies are needed to assess the epidemiology of these infections in canine population, especially with respect to the reservoirs of Trypanosoma spp. in rural areas.

Ações educativas para o controle de vetores da dengue e leishmaniose visceral

Results of educative actions on the control of vectors for dengue and leishmaniasis were evaluated by using an online semi-present course directed to teachers from Fundamental I Education level in Araçatuba-SP. 40 teachers from municipal schools located on geographical areas of town with higher occurrence of dengue and leishmaniasis cases, attended to the course, which consisted in a conceptual part directed to specific subjects such as vector borne and zoonotic diseases, responsible ownership of pets and health education and a practical phase, directed to execution and application of educative projects in the target schools. Data about the teacher’s specific knowledge, before and after the attendance to the course, were submitted to the Wilcoxon test with a 5% of significant level. The application of practical educative projects was evaluated by multiplicative actions involving target public. Tests showed statistically significant increase in the knowledge about the studied diseases (p<0.0001) after the course, especially on questions related to etiological agent, clinical symptoms on man and animals and prevention. Activities of knowledge multiplication envolved teachers and students which detected the problems and promoted education actions in their neighborhood as stage performing, group cleaning activities and elaboration and distribution of folders during a student pared. In conclusion, educative actions direct to the control of diseases vectors using a semi-present course, provides new knowledge to the teachers, impelling them to motivate their students to adopt vectors control measures and environmental cares and to sensitive the community to collaborate with control diseases.

Clinical and epidemiological aspects of feline leishmaniasis in Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)