Níveis de lisina digestível para poedeiras Hy-Line W-36 em produção

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Composição tecidual da carcaça e perfil de ácidos graxos da carne de cordeiros terminados a pasto ou em confinamento

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Structural biology of membrane-acting peptides: Conformational plasticity of anticoccidial peptide PW2 probed by solution NMR

The bottleneck for the complete understanding of the structure-function relationship of flexible membrane-acting peptides is its dynamics. At the same time, not only the structure but also the dynamics are the key points for their mechanism of action. Our model is PW2, a TRP-rich, cationic peptide selected from phage display libraries that shows anticoccidial activity against Eimeria acervulina. In this manuscript we used a combination of several NMR techniques to tackle these difficulties. The structural features of the membrane-acting peptide PW2 was studied in several membrane mimetic environments: we compared the structural features of PW2 in SDS and DPC micelles, that were reported earlier, with the structure properties in different lipid vesicles and the peptide free in water. We were able to unify the structural information obtained in each of these systems. The structural constraints of the peptide free in water were fundamental for the understanding of plasticity necessary for the membrane interaction. Our data suggested that the WWR sequence is the region responsible for anchoring the peptide to the interfaces, and that this same region displays some degree of conformational order in solution. For PW2, we found that affinity is related to the aromatic region, by anchoring the peptide to the membrane, and specificity is related to the N- and C-termini, which are able to accommodate in the membrane due to its plasticity. (C) 2007 Elsevier B.V. All rights reserved.

Developing a research agenda for promoting physical activity in Brazil through environmental and policy change

Objective. To identify the highest priorities for research on environmental and policy changes for promoting physical activity (PA) in Brazil; to uncover any gaps between researchers' and practitioners' priorities; and to consider which tools, methods, collaborative strategies, and actions could be useful to moving a research agenda forward.Methods. This was a mixed-methods study (qualitative and quantitative) conducted by Project GUIA (Guide for Useful Interventions for Activity in Brazil and Latin America) in February 2010-January 2011. A total of 240 individuals in the PA field (186 practitioners and 54 researchers) were asked to generate research ideas; 82 participants provided 266 original statements from which 52 topics emerged. Participants rated topics by "importance" and "feasibility;" a separate convenience sample of 21 individuals categorized them. Cluster analysis and multidimensional scaling were used to create concept maps and pattern matches.Results. Five distinct clusters emerged from the concept mapping, of which " effectiveness and innovation in PA interventions" was rated most important by both practitioners and researchers. Pattern matching showed a divergence between the groups, especially regarding feasibility, where there was no consensus.Conclusions. The study results provided the basis for a research agenda to advance the understanding of environmental and policy influences on PA promotion in Brazil and Latin America. These results should stimulate future research and, ultimately, contribute to the evidence-base of successful PA strategies in Latin America.

Anti-inflammatory intestinal activity of Abarema cochliacarpos (Gomes) Barneby & Grimes in TNBS colitis model

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Morphology and topography analysis of mesoporous titania templated by micrometric latex sphere arrays

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Relação lisina digestível: proteína digestível em rações para tilápias-do-nilo

This study was carried out to determine the best digestible protein/digestible lysine ratio that should be present in feed fed to Nile tilapias. Two hundred and sixteen tilapias (11.0 +/- 0.43g) were distributed in 36 fish tanks (205 L) at a density of 6 fish/tank. Twelve feeds were formulated with three different digestible protein (DP) levels 22.0; 26.0 and 30.0% (based on digestible amino acids) and four different lysine percentages of 4.5; 6.0; 7.5 or 9.0% in relation to digestible protein. The fish were fed ad libtum during a 60-day period. There was significant effect of the digestible protein and digestible lysine ratio on weight gain, because the increase in lysine levels in feeds with 26 and 30% DP promoted linear increase in this parameter. The best values for feed conversion were obtained at the levels 26 and 30% DP that increased the daily consumption of digestible protein. The lysine level caused a linear reduction in feed conversion and linear increase in the protein efficiency rate. The results suggested that the 26% DP level might be used in ration to feed Nile tilapia juveniles; however, these should contain digestible amino acids and the digestible lysine/digestible protein ratio should be 6.0%. However, for levels higher than 26% DP, a maximum digestible lysine level of 7.5% DP improved weight gain for the species.

Figuras femininas e fronteiras sociais na poética de Manoel de Barros

We contemplated concerning the borders in the social relationships, verifying that way the speech poetic internalizes the moral conventions and it fastens identities when proposing the alterity as a one other radically different. We studied the woman's representation in the poetry of Manoel de Barros, in the grandmother's figures, of the mother, of the women of the people and of the prostitutes. We verified the way as the women are figured and the function that they carry out in the universe of poetic's Barros.

Desempenho produtivo de tilápia do Nilo alimentada com níveis de colina na dieta

Growth performance of Nile tilapia fed graded choline levels in the diet. A 109-day feeding trial was undertaken aiming to evaluate the growth performance of Nile tilapia fed graded choline levels One hundred and ninety-two (initial-weight 4 0 [plus or minus] 0 15 g) fingerlings were distributed into 32 net cages (200 L each), four cages per treatment and six fish per cage, placed in eight 10001, aquaria in a closed recirculation system The treatments were assigned to the tanks comprising eight treatments and loin replications arranged in a completely randomized experimental design Diets were supplemented with choline chloride to provide 100, 200, 400, 600, 800, 1000, 1200 mg of choline per kg of feed and an unsupplemented diet No significant differences were observed in growth performance, survival, apparent feed conversion, liver and fillet ether extract, hepatosomatic index and plasma lipid concentration, among treatments Choline levels did not improve growth performance, possibly because the amount of choline in the diet had already met fish requirement

Morfologia do figado da paca (Cuniculus Paca, Linnaeus 1766)

The elements related to the morphology of the liver of paca (Cuniculus paca), the second largest rodent of the Brazilian fauna, were observed; this species present zootechnical potential. Eight animals from the animals sector of Faculdade de Ciencias Agrarias e Veterinarias - Campus of Jaboticabal - UNESP, which is duly certified by IBAMA as an experimental breeding institute, were used. Through a dissection procedure, it was found that the liver of the paca is located in the cranial portion of the abdomen, immediately after the diaphragm, to which it is connected by the triangular, coronary, and falciform ligaments, having its bigger part located right to the medium plan. The liver of this rodent presents the following lobation: right lateral lobe, right medial lobe, quadrate lobe, left medial lobe, and left lateral lobe, besides the caudate lobe formed by the papillary process of caudate lobe and the caudate process of caudate lobe. Gallbladder is located between the quadrate and right medial lobes. Fragments of this organ were collected, fixed, and histologically prepared, being the samples analyzed through light microscopy. It was microscopically observed that intralobular connective tissue is scarce, basically it consists polyhedral hepatocytes organized into cords interposed between sinusoids and the portal triads are found in the lobe, consisting of the portal vein, hepatic artery, and biliary duct.

Short-chain di-ureasil ormolytes doped with potassium triflate: Phase diagram and conductivity behavior

Di-urea cross-linked poly(oxyethylene)/siloxane hybrids, synthesized by the sol-gel process and containing a wide concentration range of potassium triflate, KCF3SO3, have been analyzed by x-ray diffraction and differential scanning calorimetry. The pseudo-phase diagram proposed has been taken into account in the interpretation of the complex impedance measurements. The xerogels prepared are obtained as transparent, thin monoliths. At room temperature the highest conductivity found was 2 × 10-6 Ω-1 cm-1.

Liposomal daunorubicin and dexamethasone as a treatment for multiple myeloma - The DD protocol

Context and Objective: Lipasomial daunorubicin has been used to treat hematological malignancies, including multiple myelomo (MM). The goal was to evaluate efficacy, side-effects and toxicity of liposomal daunorubicin and dexamethasone (DD Protocol). Design and Setting: Prospective study of Sírio-Libonês, São Camilo, Brasil and Alemão Oswaldo Cruz hospitals. Methods: Twenty consecutive patients with active MM received four cycles of liposomal daunorubicin intravenously for two hours (25-30 mg/m 2/day) on three consecutive days per month, with oral dexamethasone, (10 mg every six hours) on four consecutive days three times a month. Results: The male/female ratio was 1:1 and median age 60. Nine patients were stage IIA, ten IIIA and one IIIB. The median from diagnosis to starting DD was 13 months. All patients received four cycles, except one. Fifteen had already received chemotherapy before DD. Responses of > 50% reduction in serum monoclonal paraprotein were observed in six patients after first cycle (30%), six after second (30%) and four after third (20%), while four (20%) did not obtain this. Initially, 17 patients (85%) had anemia: 12 (70%) achieved correction. Progressive disease was observed in three patients (15%), while one had minimal response, four (20%) partial and 12 (60%) complete. Hemotologlical toxicity was acceptable: three patients (15%) had neutrophils < 1,000/mm 3; none had thrombocyfopenia. Gastrointestinal toxicity was mild: nausea (10%), anorexio (15%) and no vomiting. Conclusions: This treatment has mild toxicity and good response rate. It may therefore be feasible before autologous bone marraw transplantation.

Precisão de plantas planialtimétricas em função do posicionamento de pontos em irradiadas de campo

The present experiment aimed to study the influence of point positioning in an irradiated field to produce a planialtimetric plant. A planialtimetric evaluation was carried out in a 14-acre experimental area with well-defined topographic variations. Planialtimetric maps were designed using manual procedures, Datageosis and Topoesalq. Datageosis built all the curves after numerical surface modeling. Topoesalq provided only height reports and the drawing of curves was done manually. The third method was a manual procedure. Because there were planialtimetry representation differences, longitudinal profiles were used in the sites where there was a great divergence among plants. When obtained profiles and plants were compared, it was verified that the one produced by Datageosis represented the relief plant better. Later, only the irradiated field points were evaluated and each point presented positioned readings before and after each relief map variation. The processing through the three methods resulted in significant plants of the local planialtimetry, according to the control profiles. It was concluded that the planning of the field procedure should be suitable to the posterior treatment method of obtained data in order to make a planialtimetric plant to accord to the evaluated local topography.