Increased expression and activity of sodium channels in alveolar type II cells of hyperoxic rats.

We investigated the cellular and molecular events associated with the increase in sodium transport across the alveolar epithelium of rats exposed to hyperoxia (85% O2 for 7 days followed by 100% O2 for 4 days). Alveolar type II (ATII) cell RNA was isolated and probed with a cDNA for one of the rat colonic epithelial sodium channel subunits (alpha rENaC). The alpha rENaC mRNA (3.7-kb transcript) increased 3-fold in ATII cell RNA isolated from rats exposed to 85% O2 for 7 days and 6-fold after 4 days of subsequent exposure to 100% O2. In situ hybridization revealed increased expression of alpha rENaC mRNA transcripts in both airway and alveolar epithelial cells of hyperoxic rats. When immunostained with a polyclonal antibody to kidney sodium channel protein, ATII cells from hyperoxic rats exhibited a significant increase in the amount of immunogenic protein present in both the plasma membrane and the cytoplasm. When patched in the whole-cell mode, ATII cells from hyperoxic rats exhibited amiloride and 5-(N-ethyl-N-isopropyl)-2',4'-amiloride (EIPA)-sensitive currents that were 100% higher compared with those obtained from air-breathing rats. Single-channel sodium currents (mean conductance of 25 pS) were seen in ATII cells patched in both the inside-out and cell-attached modes. The number and open probability of these channels increased significantly during exposure to hyperoxia. Exposure to sublethal hyperoxia up-regulated both alpha rENaC mRNA and the functional expression of sodium channels in ATII cells.

Student notebook of Ebenezer Parkman, 1720

The small hardcover notebook contains a manuscript copy of Charles Morton's Natural Philosophy copied by student Ebenezer Parkman (Harvard Class of 1721) in 1720, as well as notes on Hebrew grammar. The flyleaf has a faded note, "[This copy] was probably made by Parkman H.U. 1721 afterward minister of Westboro." The title page of the volume includes the handwritten title "Phylosophia Natvralis: Naturall Philosophy, By the Reverd Mr. Charles Morton Pastor of a Church in Charles Town, Beegan [sic] to recite it December 11, 1720 Willm Brattle's Book 1720 ended January 30 Anno Domini 1720 [January 30, 1720/1721]." The final page of the transcription is signed and dated "June 18, 1720 Parkman." The last pages of the volume consist of notes on Hebrew Grammar titled "Instruction in Hebrew."

Annexe II: Analyse de la situation politique en Mediterranee et dans les pays du golfe el les risques sur les approvisionnements petroliers et gaziers 1) Algerie & Pays D'Afrique du Nord 2) Pays de L'Est Mediterraneen et du Golfe. OME, Draft du 18 April 1995 = Annex II: Analysis of the political situation in the Mediterranean and the Gulf countries and risks on petroleum and gas supplies 1) Pays D'Algerie & North Africa 2) Country of Eastern Mediterraneen and the Gulf. OME, Draft of 18 April 1995

None.