Análise do envolvimento do fator de início de tradução de eucariotos 5A (eIF5A) na translocação de proteínas para o retículo endoplasmático em Saccharomyces cerevisiae

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Recriação conceitual: reflexões sobre a ressignificação da obra de arte como um fator criativo

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Influência da adição de fator de crescimento fibroblástico 10 (FGF10), durante a maturação oocitária, na produção in vitro de embriões bovinos

The Brazilian livestock stands out for having the world largest commercial herd of cattle and leads meat exportation and production of bovine embryos. The in vitro production (IVP) of embryos is considered an effective option to overcome problems such as infertility in cows with high economic value and also for genetic improvement of cattle. The in vitro oocyte maturation is an essential step to the success of IVP, but is still considered poor when compared to in vivo maturation. Recent studies have suggeested an important role of Fibroblast Growth Factor 10 (FGF10) on the in vitro maturation of oocytes, which favored the expression of genes related to oocyte maturation and cumulus cell expansion. Aware that maturity stage influences the final production of blastocysts, we aimed study to verify if the addition of FGF10 into the maturation medium is able to affect positively the IVP of bovine embryos. Hence, FGF10 was added to maturation in five different concentrations: 0.5 ng/mL (group 0.5), 2.5 ng/mL (group 2.5), 5 ng/mL (group 5), 10 ng/mL (group 10) and 50 ng/mL (group 50). Additionally, two other maturation groups were used, group BSA (Bovine Serum Albumin, 4 mg/mL) and group FCS (Fetal Calf Serum, 10%). The rates of cleavage, morula and blastocyst were analyzed by Analysis of Variance (ANOVA), differences of P<0.05 were considered significant. Cleavage rates did not differ between the seven groups. On the other hand, morula rate on FCS group was higher than groups BSA, 0.5, 10 and 50 (P<0.05), but did not differ among groups treated with intermediate doses of FGF10 (2.5 and 5). FCS group presented higher blastocyst rate compared to all other groups that were well below the FCS group (P<0.0001). Therefore, the use of FGF10 during oocyte maturation did not affect positively embryo development on the IVP of bovine embryos

Determinação de adenosina deaminase, fator de necrose tumoral-alfa e interleucina-1 em gestantes portadoras de pré-eclâmpsia

Preeclampsia is a specific disorder of pregnancy, characterized by arterial hypertension and proteinuria detected after 20 weeks of gestation. This pathology is associated with hyperuricemia, higher levels of pro-inflammatory cytokines, enhanced leukocyte activation and oxidative stress. Adenosine deaminase (ADA) is an enzyme present in all human tissues and, it is involved with the maturation of the immune system. Although its function is not fully understood, ADA is considered an indicator of cellular inflammation and, its increased serum concentration is observed in inflammatory diseases, such as tuberculosis and rheumatoid arthritis. This study aimed to assess serum ADA levels in preeclamptic patients (PE) compared with normotensive pregnant (NT) and non-pregnant women (NP), and to correlate these values with TNF-α and IL-1β production. Ninety pregnant women were included: 60 were pre-eclamptic and 30 were normotensive matched for gestational age. As control group 20 healthy non-pregnant women matched with pregnant for age were included. Peripheral blood mononuclear cells (PMMC) obtained from the three groups studied were cultured with or without lipopolysaccharide (LPS) for 18h at 37oC, and TNF-α and IL-1β production was assessed in the supernatant of cultures by enzyme immunoassay (ELISA). ADA plasmatic concentration was determined by colorimetric method. The results show that ADA plasma levels were significantly higher in PE group compared with NT and NP groups. A positive correlation between ADA and uric acid levels was detected in preeclamptic women. There was no significant difference in relation to ADA levels when PE patients were classified in early and late-onset PE. The endogenous production of IL-1β and TNF-α by PBMC was significantly higher in PE group than in NT and NP women, showing the activation state of these cells in PE. LPS induced...(Complete abstract click electronic access below)

Efeito da silibinina sobre o fator de transcrição nuclear-kB e produção de citocinas inflamatórias por células mononucleares de gestantes portadoras de pré-eclâmpsia

Pre-eclampsia (PE) is a complication of human pregnancy characterized by hypertension and proteinuria after 20 weeks of gestation. Its incidence varies from 5% to 7% of pregnancies and is a major cause of morbidity and maternal and fetal mortality. This is a multisystemic disease, with focus on vascular dysfunction and is closely related to the exacerbated activation of the immune system. In addition to increased activation of monocytes and granulocytes, there is an elevated production of proinflammatory cytokines in pregnant women with PE. The nuclear transcription factor-kB (NF-kB) is present in the cells of the immune system and is responsible for transcription of genes related to inflammation. Whereas the PE is associated with intense inflammatory response, the use of substances modulating the activity of NF-kB factor could be useful in alleviating the inflammation present in these patients. Silibinin is the main component of silymarin, a polyphenolic extract obtained from fruits and seeds of Sylibum marianum with potent hepatoprotective, anti-inflammatory and anti-fibrotic activities. The silibinin mechanism of action includes the ability to inhibit NF-kB activation and, consequently, its migration to the nucleus. The objective of this study was to assess whether silibinin modulates the activity of NF-kB and the production of inflammatory cytokines in mononuclear cells of patients with PE. We evaluated 34 pregnant women with PE, 20 normotensive pregnant women (GN) and 15 non-pregnant women (NG). Peripheral blood mononuclear cells (PBMC) were obtained from those groups of women and cultured in the presence or absence of silibinin (5 uM or 50 uM) and stimulated or not with lipopolysaccharide (LPS) for 18 h to obtain supernatant for determination of tumor necrosis factor-alpha (TNF-α) and interleukin-1 (IL-1β) by enzyme immunoassay (ELISA). The cells were...(Complete abstract click electronic access below)

A comunicação interna como fator de inovação nas Micro e Pequenas Empresas

The work presents a literature review on the topic of internal communication as an innovation factor in Micro and Small Business. The objective is to discuss how internal communication can be an essential factor in the pursuit of innovation and the consequent competitive advantage in the Micro and Small Enterprises scenario. We present the concepts of integrated communication, organizational communication, internal communication, whether crafted by a philosophy of Public Relations, can lead these companies to gain competitive advantage and overcome the limitations and challenges imposed on them

Fator de condição e biologia alimentar de Pimelodus maculatus em três pontos do Rio Piracicaba (SP)

A number of 170 individuals of the species Pimelodus maculatus, a Neotropical fish known as mandi was collected in three distinct locations of the Piracicaba river (Piracicaba, Santa Maria da Serra and Americana – SP0 from April 2009 to February 2010. A common species of catfish whose individuals exist in many neotropical basins was observed to get more information about a possible influence of the water conditions in distant parts of a same river to its condition and also to verify whether the stomach contents of fish collected in these distinct places may distinguish as a possible consequence of food availability. The species were studied by collecting biological data such as standard length (SL) in cm, their total weights (TW ) in grams and sexes to see the composition of fish present in the samples. Length-weight relationships are important means to investigate facts related to the condition of the species in these systems. The analysis was made by using covariance to observe if individuals of similar lengths collected in distinct places may be compared to this purpose. Usual methods to investigate relationships of stomach food contents were applied. By analyzing the condition factor, one may conclude that the mandis collected in the region of Santa Maria da Serra presented similar characteristics to those obtained in Piracicaba, but they differ in this aspect for individuals collected in Americana. This fact is not conclusive about the reasons of this situation, but it may be a first step to other studies in order to get more information about. The degree of stomach repletion may be a first approach to this possible explanation, as individuals of Piracicaba presented usually full stomachs and differed in this way to individuals collected in the two other places

Otimização da extração alcalina com peróxido de hidrogênio em seqüência de branqueamento com aplicação de baixo fator kappa

The new market, focused on sustainability and other environmental concerns, refers to innovations that seek alternative forms of production. In pulp and paper bleaching alternative reagents are studied, for example, hydrogen peroxide, in partial substitution of chlorine dioxide in order to reduce the formation of organochlorines. In this context, this study examined the burden of hydrogen peroxide (H2O2) on alkaline extraction stage (stage Ep) required for the bleaching of pulp with eucalyptus kraft pulp, pre-oxygen delignified to obtain equivalent brightness at 90 ± 0.5% ISO, as well as its effect on quality of pulp produced. The pulp was bleached by the sequence D(Ep)DP, with the application of factor kappa of 0.14 and varying the concentration of hydrogen peroxide in Ep stage three, five, seven and nine kilograms of reagent per ton of pulp absolutely drought. The final P stage was optimized with the use of six, nine and twelve pounds of hydrogen peroxide per ton of absolutely dry pulp to achieve the required brightness. The quality of the pulp produced was analyzed based on the kappa number, the brightness and the viscosity. The methods were performed according to standards set by the standard TAPPI (Technical Association of the Pulp and Paper Industry). The best result was obtained using the following D0Ep(7)D1P(6), which showed a viscosity of 19.9 cP, 89.6% ISO brightness, consumption of 94.9 kg / t of reagents and reagent costs of US$ 28.15, because it showed better pulp quality for a lower cost compared to the others. It was found that the greater the amount of hydrogen peroxide in alkaline extraction, the lower the kappa number and increased the amount of residual hydrogen peroxide. The higher the charge of hydrogen peroxide in Ep stage, the lower the need for hydrogen peroxide in the final P stage, reducing the cost of bleaching

Estudo da relação entre o fator de início de tradução de eucariotos 5A (eIF5A) e a etapa de elongação da tradução

O fator de início de tradução 5A (eukaryotic translation iniciation factor 5A, eIF5A) é altamente conservado entre arqueas a eucariotos, sendo que as proteínas eIF5A de Saccharomyces cerevisiae e de mamíferos são 63% idênticas. eIF5A sofre uma modificação pós-traducional única na célula, a hipusinação de um resíduo de lisina. Essa proteína já foi relacionada ao início da tradução, transporte nucleocitoplasmático, decaimento de mRNA e proliferação celular, mas a função crítica de eIF5A ainda não foi esclarecida. A depleção deste fator em S. cerevisiae leva a uma diminuição (30%) da taxa de síntese protéica, sugerindo que eIF5A seja um fator envolvido na tradução de um grupo específico de mensageiros. Dados do laboratório demonstram interação física entre eIF5A e proteínas ribossomais bem como com o fator de elongação 2 da tradução (eEF2). A interação com eEF2, sugere que eIF5A atua na etapa de elongação da tradução, ao invés do início da tradução, como proposto inicialmente. Com o objetivo de avaliar a relação de eIF5A com a etapa de elongação, foram realizadas análises de interações genéticas entre o gene codificador de eIF5A (TIF51A) e diversos genes codificadores de proteínas envolvidas na tradução. Através de análises de interações genéticas, foi observado que o mutante estável de eIF5A, tif51AK56A, apresenta um defeito de crescimento quando o mutante de eEF2, eft2H699K, está expresso em alto número de cópias, enquanto que o mutante tif51AQ22H/L93F não apresenta defeitos nesta condição. Foi observado também que o mutante tif51AQ22H/L93F apresenta um defeito de crescimento mais severo quando ocorre superexpressão de EFT2, gene codificador de eEF2. Foi observado ainda que não há complementação alélica entre os mutantes estáveis de eIF5A e que a reversibilidade do fenótipo de sensibilidade a temperatura... (Resumo completo, clicar acesso eletrônico abaixo)

Reforma Agrária em questão: a propriedade privada como fator de desterritorialização camponesa no Assentamento Fazenda Primavera (Andradina-SP)

Pós-graduação em Geografia - FCT

Fator de crescimento do endotélio vascular e do transportador de glicose 1 em cães com ceratose actínica e carcinoma de células escamosas

Pós-graduação em Medicina Veterinária - FCAV

Estabilidade do fator de von Willebrand e fator VIII no crioprecipitado canino em diferentes protocolos de armazenamento

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Caracterização do fator de intensidade de tensão em corpos trincados através do método dos elementos finitos

The industry generally has sought materials with high mechanical resistance, low density, thermal stability and corrosion resistance. In the aerospace industry, for example, the use of aluminum alloys, such as Al 2024-T351 and Al 7075-T7351, have become essential. However, the use of these materials often do not resulted in a satisfactory performance of the component, since the presence of cracks can cause total rupture of the component, even with a tension below the yield stress of the material, unexpectedly. In this work, these aluminum alloys were analyzed and samples were modeled by the finite element method. Moreover, in the models were applied two different types of cracks, central and edge crack, a vertical force was applied to result in a tension 70% of the yield stress of the material analyzed. Through stress asymptotic distribution in the region near the crack tip were calculated the values of the stress intensity factors for each crack length, after the stress intensity factors characterized were compared graphically with the values of fracture toughness found in the available literature

Usabilidade: um fator crítico para da interatividade da televisão digital

Este artigo tem por objetivo fazer uma reflexão da usabilidade aplicada à interatividade do SBTVD (Sistema Brasileiro de Televisão Digital). Aborda as perspectivas de mercado no Brasil e na América Latina e cita alguns casos em que a usabilidade constituiu-se em fator decisivo para ampliar o ciclo de vida do produto. Explicita a interação e a engenharia de usabilidade com destaque para a garantia da consolidação da interatividade do SBTVD. Neste contexto, inclui o estabelecimento de redes de banda larga para todos, chamados de “cidade digital” condição decisiva no processo de possibilitar a democratização. Conclui que essa facilidade de usabilidade, aliada aos avanços tecnológicos digitais que propiciam uma qualidade e uma convergência tecnológica, que certamente romperá o modelo de telespectador passivo para um telespectador interativo gerando um novo contingente de telespectadores vindo da televisão digital e da televisão analógica.

Efeitos do ácido ascórbico intracameral sobre o endotélio corneal e sobre o fator de necrose tumoral alfa (TNF-a) em cães submetidos à facoemulsificação

Pós-graduação em Cirurgia Veterinária - FCAV