Diseases and parasites of penaeid prawns of India: a short review

The diseases caused by bacteria, fungi, protozoa and metazoa as well as by other biotic and abiotic agents reported in the penaeid prawns of India are reviewed.

Studies on the fungal diseases in crustaceans. I. Lagenidum scyllae sp. nov. isolated from cultivated ova and larvae of the mangrove crab (Scylla serrata)

Lagenidium scyllae , a marine mastigomycete from the ova and larvae of the mangrove crab, is described and illustrated as new. The fungus grew at a temperature range of 16-42 C, with an optimum at 22.5-31.8 C. It grew well in peptone-yeast-glucose (PYG) broth containing 2-3% NaCl. In PYG-sea water medium, it grew at a pH range of 5-11.

Containing the diseases in aquaculture

A brief discussion is presented on epizootics and their containment in the aquaculture industry, in the form of a question-and-answer interview. Particular reference is made to activities carried out by the OIE (Office International des Epizooties), whose main activities include the provision of guidelines and standards for health protection applicable to international trade in live animals and their products. It has devised an early warning system to apprise its member countries of the occurence of disease outbreaks that would have serious repercussions on public health or the economics of animal products. URI

Parasitic fish diseases and their impact on potential fish production in East Africa

Protein deficient diets are a standard way of life in many parts of East Africa;this of course tends to result in shorter life expectancy and chronic ill-health. Population increase is sufficiently high to outdistance the economic gains that may be made in various fields. With recurrent shortages of basic commodities not only in East Africa, but in many parts of the world, it is becoming increasingly clear that agricultural production practices must be maximised rapidly in order to meet the world's constantly expanding need for food. Here in East Africa, while our food requirements can be met most of the time, our protein requirements are far from being met. Yields from traditional fishery resources, must therefore be increased. The farming of fish (aquaculture)adds a new dimension to food production in general and high quality protein production in particular, in that it can be incorporated into other agricultural production activities.

Gelatin nanofiber-reinforced alginate gel scaffolds for corneal tissue engineering.

A severe shortage of donor cornea is now an international crisis in public health. Substitutes for donor tissue need to be developed to meet the increasing demand for corneal transplantation. Current attempts in designing scaffolds for corneal tissue regeneration involve utilization of expensive materials. Yet, these corneal scaffolds still lack the highly-organized fibrous structure that functions as a load-bearing component in the native tissue. This work shows that transparent nanofiber-reinforced hydrogels could be developed from cheap, non-immunogenic and readily available natural polymers to mimic the cornea's microstructure. Electrospinning was employed to produce gelatin nanofibers, which were then infiltrated with alginate hydrogels. Introducing electrospun nanofibers into hydrogels improved their mechanical properties by nearly one order of magnitude, yielding mechanically robust composites. Such nanofiber-reinforced hydrogels could serve as alternatives to donor tissue for corneal transplantation.

Staphylococcus aureus lipoproteins trigger human corneal epithelial innate response through toll-like receptor-2

Bacterial lipoproteins (LP) are a family of cell wall components found in a wide variety of bacteria. In this study, we characterized the response of HUCL, a telomerase-immortalized human corneal epithelial cell (HCEC) line, to LP isolated from Staphylococcus (S) aureus. S. aureus LP (saLP) prepared by Triton X-114 extraction stimulated the activation of NF-kappa B, JNK, and P38 signaling pathways in HUCL cells. The extracts failed to stimulate NF-kappa B activation in HUCL cells after lipoprotein lipase treatment and in cell lines expressing TLR4 or TLR9, but not TLR2, indicating lipoprotein nature of the extracts. saLP induced the up-regulation of a variety of inflammatory cytokines and chemokines (IL-6, IL-8, ICAM-1). antimicrobial molecules (hBD-2, LL-37, and iNOS), and homeostasis genes (Mn-SOD) at both the mRNA level and protein level. Similar inflammatory response to saLP was also observed in primarily cultured HCECs using the production of IL-6 as readout. Moreover, TLR2 neutralizing antibody blocked the saLP-induced secretion of IL-6, IL-8 and hBD2 in HUCL cells. Our findings suggest that saLP activates TLR2 and triggers innate immune response in the cornea to S. aureus infection via production of proinflammatory cytokines and defense molecules. (C) 2007 Elsevier Ltd. All rights reserved.