Widespread expression and estrogen regulation of PPEIA-3′ nuclear RNA in the rat brain

We previously identified a novel nuclear RNA species derived from the preproenkephalin (PPE) gene. This transcript, which we have named PPEIA-3′ RNA, hybridizes with probes directed at a region of PPE intron A downstream of an alternative germ-cell transcription start site, but does not contain PPE protein coding sequences. We now report that estrogen treatment of ovariectomized rats increases the expression of conventional PPE heteronuclear RNA, and also induces the expression of PPEIA-3′ RNA, apparently in separate cell populations within the ventromedial nucleus of the hypothalamus. Further, we show that cells expressing PPEIA-3′ are found in several neuronal groups in the rat forebrain and brainstem, with a distinct topographical distribution. High densities of PPEIA-3′ containing cells are found in the reticular thalamic nucleus, the basal forebrain, the vestibular complex, the deep cerebellar nuclei, and the trapezoid body, a pattern that parallels the distribution of atypical nuclear RNAs described by other groups. These results suggest that this diverse neuronal population shares a common set of nuclear factors responsible for the expression and retention of this atypical RNA transcript. The implication of these results for cell-specific gene transcription and regulation in the brain and the possible relationship of PPEIA-3′ RNA and other atypical nuclear RNAs is discussed.

Induction and repression of DAN1 and the family of anaerobic mannoprotein genes in Saccharomyces cerevisiae occurs through a complex array of regulatory sites

The DAN/TIR mannoprotein genes of Saccharomyces cerevisiae (DAN1, DAN2, DAN3, DAN4, TIR1, TIR2, TIR3 and TIR4) are expressed in anaerobic cells while the predominant cell wall proteins Cwp1 and Cwp2 are down-regulated. Elements involved in activation and repression of the DAN/TIR genes were defined in this study, using the DAN1 promoter as a model. Nested deletions in a DAN1/lacZ reporter pinpointed regions carrying activation and repression elements. Inspection revealed two consensus sequences subsequently shown to be independent anaerobic response elements (AR1, consensus TCGTTYAG; AR2, consensus AAAAATTGTTGA). AR1 is found in all of the DAN/TIR promoters; AR2 is found in DAN1, DAN2 and DAN3. A 120 bp segment carrying two copies of AR1 preferentially activated transcription of lacZ under anaerobic conditions. A fusion of three synthetic copies of AR1 to MEL1 was also expressed anaerobically. Mutations in either AR1 site within the 120 bp segment caused a drastic loss of expression, indicating that both are necessary for activation and implying cooperativity between adjacent transcriptional activation complexes. A single AR2 site carried on a 46 bp fragment from the DAN1 promoter activated lacZ transcription under anaerobic conditions, as did a 26 bp synthetic AR2 fragment fused to MEL1. Nucleotide substitutions within the AR2 sequence eliminated the activity of the 46 bp segment. Ablation of the AR2 sequences in the full promoter caused a partial reduction of expression. The presence of the ATTGTT core (recognized by HMG proteins) in the AR2 sequence suggests that an HMG protein may activate through AR2. One region was implicated in aerobic repression of DAN1. It contains sites for the heme-induced Mot3 and Rox1 repressors.

Paris, France, 1367-1383 (Time Series Map 5 of 8) (Raster Image)

This layer is a georeferenced raster image from the historic paper map series entitled: Lutece ... plan de la ville de Paris ..., par M.L.C.D.L.M. ; A. Coquart, delineavit et sculp. It was published by Jean & Pierre Cot in 1705. Scale [ca. 1:10,000]. This image is of map 5 entitled: Cinquiême plan de la ville de Paris son accroissement et sa quatriême clôture commancée sous Charles V. l'an 1367 et finie sous Charles VI. l'an 1383: tiré des devis et marchez faits avec le ouvriers, des procez verbaux de toisez et receptions des ouvrages des comptes rendus par ceux qui en eurent la conduite, de la chronique m.s. de St. Denis et d'autres titres et manuscrits qui sont conservez en la Chambre des Comptes et dans Biblioteques. The map represents Paris, 1367 to 1383. Map in French.The image inside the map neatline is georeferenced to the surface of the earth and fit to the European Datum 1950, Universal Transverse Mercator (UTM) Zone 31N projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, towns and villages, roads, built-up areas and selected buildings, fortification, ground cover, and more. Relief shown by hachures. Includes index, text, and notes.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Die galvanoplastik für künstler, gewerbtreibende und freunde der numismatik ... Nebst ergänzenden zusätzen des uebersetzers.

Errors in paging.

Cost-effectiveness of countermeasures for utility pole accidents. Final report.

Federal Highway Administration, Office of Research and Development, Washington, D.C.

Traffic operations control for older drivers. Final report.

Federal Highway Administration, Office of Safety and Traffic Operations Research and Development, McLean, Va.

Chroniques de J. Froissart,

Vol. 8, pt. II, to vol. 11, edited by Gaston Raynaud; vol. 12- by Léon Mirot.

Safety effects of cross-section design for two-lane roads. Volume I - final report.

Federal Highway Administration, Safety Design Division, McLean, Va.

Safety cost-effectiveness of incremental changes in cross-section design - information guide.

Turner-Fairbank Highway Research Center, McLean, Va.

The operation of larger trucks on roads with restrictive geometry. Volume I. Final report.

Federal Highway Administration, Office of Safety and Traffic Operations Research and Development, McLean, Va.

Selection of cost-effective countermeasures for utility pole accidents - user's manual.

Federal Highway Administration, Office of Implementation, McLean, Va.

PEDSAFE : pedestrian safety guide and countermeasure selection system /

"September 2004."

The operation of larger trucks on roads with restrictive geometry. Volume II - appendixes.

Federal Highway Administration, Office of Safety and Traffic Operations Research and Development, McLean, Va.

Design risk analysis. Volume I: final report. Final report.

Federal Highway Administration, Office of Safety and Traffic Operations Research and Development, McLean, Va.